UniProtKB - Q1RP79 (PA2B1_VIPBN)
Basic phospholipase A2 chain HDP-1P
Functioni
Heterodimer: shows the same activities as the monomer, but with a lower potency.
Monomer: snake venom phospholipase A2 (PLA2) that shows presynaptic neurotoxicity, anticoagulant activity and that weakly inhibits ADP-induced platelet aggregation (PubMed:18083205).
Inhibits exocytosis in pancreatic beta cells, confirming it can act presynaptically in inhibiting the exocytosis of neurotransmitters in neurons (PubMed:19500614).
PLA2 catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides.
2 PublicationsCatalytic activityi
- a 1,2-diacyl-sn-glycero-3-phosphocholine + H2O = a 1-acyl-sn-glycero-3-phosphocholine + a fatty acid + H+PROSITE-ProRule annotationEC:3.1.1.4PROSITE-ProRule annotation
Cofactori
Activity regulationi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 43 | Calcium; via carbonyl oxygenBy similarity | 1 | |
Metal bindingi | 45 | Calcium; via carbonyl oxygenBy similarity | 1 | |
Metal bindingi | 47 | Calcium; via carbonyl oxygenBy similarity | 1 | |
Active sitei | 63 | By similarity | 1 | |
Metal bindingi | 64 | CalciumBy similarity | 1 | |
Active sitei | 105 | By similarity | 1 |
GO - Molecular functioni
- calcium ion binding Source: InterPro
- phospholipase A2 activity Source: UniProtKB-EC
- toxin activity Source: UniProtKB-KW
GO - Biological processi
- arachidonic acid secretion Source: InterPro
- lipid catabolic process Source: InterPro
- phospholipid metabolic process Source: InterPro
Keywordsi
Names & Taxonomyi
Protein namesi | Recommended name: Basic phospholipase A2 chain HDP-1P (EC:3.1.1.4)Short name: svPLA2 Alternative name(s): Heterodimeric neurotoxic phospholipases A2 basic subunit 1 Phosphatidylcholine 2-acylhydrolase |
Organismi | Vipera berus nikolskii (Nikolsky's adder) (Vipera nikolskii) |
Taxonomic identifieri | 1808362 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Lepidosauria › Squamata › Bifurcata › Unidentata › Episquamata › Toxicofera › Serpentes › Colubroidea › Viperidae › Viperinae › Vipera › |
Subcellular locationi
Extracellular region or secreted
Extracellular region or secreted
- extracellular region Source: UniProtKB-SubCell
Keywords - Cellular componenti
SecretedPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 16 | 1 PublicationAdd BLAST | 16 | |
ChainiPRO_5000079752 | 17 – 138 | Basic phospholipase A2 chain HDP-1PAdd BLAST | 122 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 42 ↔ 131 | 1 Publication | ||
Disulfide bondi | 44 ↔ 60 | 1 Publication | ||
Disulfide bondi | 59 ↔ 111 | 1 Publication | ||
Disulfide bondi | 65 ↔ 138 | 1 Publication | ||
Disulfide bondi | 66 ↔ 104 | 1 Publication | ||
Disulfide bondi | 73 ↔ 97 | 1 Publication | ||
Disulfide bondi | 91 ↔ 102 | 1 Publication |
Keywords - PTMi
Disulfide bondExpressioni
Tissue specificityi
Interactioni
Subunit structurei
Heterodimer; non-covalently linked. The toxic basic protein has phospholipase A2 activity (chain HDP-1P) and the non-toxic acidic protein functions as its inhibitor (chain HPD-1I (AC A4VBF0)).
Structurei
Secondary structure
3D structure databases
SMRi | Q1RP79 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q1RP79 |
Family & Domainsi
Sequence similaritiesi
Keywords - Domaini
SignalFamily and domain databases
CDDi | cd00125, PLA2c, 1 hit |
Gene3Di | 1.20.90.10, 1 hit |
InterProi | View protein in InterPro IPR001211, PLipase_A2 IPR033112, PLipase_A2_Asp_AS IPR016090, PLipase_A2_dom IPR036444, PLipase_A2_dom_sf IPR033113, PLipase_A2_His_AS |
PANTHERi | PTHR11716, PTHR11716, 1 hit |
Pfami | View protein in Pfam PF00068, Phospholip_A2_1, 1 hit |
PRINTSi | PR00389, PHPHLIPASEA2 |
SMARTi | View protein in SMART SM00085, PA2c, 1 hit |
SUPFAMi | SSF48619, SSF48619, 1 hit |
PROSITEi | View protein in PROSITE PS00119, PA2_ASP, 1 hit PS00118, PA2_HIS, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
10 20 30 40 50
MRILWIVAVC LIGVEGNLFQ FAKMINGKLG AFSVWNYISY GCYCGWGGQG
60 70 80 90 100
TPKDATDRCC FVHDCCYGRV RGCNPKLAIY AYSFKKGNIV CGKNNGCLRD
110 120 130
ICECDRVAAN CFHQNQNTYN KNYKFLSSSR CRQTSEQC
Mass spectrometryi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AM238698 mRNA Translation: CAJ87658.1 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AM238698 mRNA Translation: CAJ87658.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2I0U | X-ray | 2.20 | A/E | 17-138 | [»] | |
SMRi | Q1RP79 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q1RP79 |
Family and domain databases
CDDi | cd00125, PLA2c, 1 hit |
Gene3Di | 1.20.90.10, 1 hit |
InterProi | View protein in InterPro IPR001211, PLipase_A2 IPR033112, PLipase_A2_Asp_AS IPR016090, PLipase_A2_dom IPR036444, PLipase_A2_dom_sf IPR033113, PLipase_A2_His_AS |
PANTHERi | PTHR11716, PTHR11716, 1 hit |
Pfami | View protein in Pfam PF00068, Phospholip_A2_1, 1 hit |
PRINTSi | PR00389, PHPHLIPASEA2 |
SMARTi | View protein in SMART SM00085, PA2c, 1 hit |
SUPFAMi | SSF48619, SSF48619, 1 hit |
PROSITEi | View protein in PROSITE PS00119, PA2_ASP, 1 hit PS00118, PA2_HIS, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | PA2B1_VIPBN | |
Accessioni | Q1RP79Primary (citable) accession number: Q1RP79 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 5, 2009 |
Last sequence update: | May 16, 2006 | |
Last modified: | February 23, 2022 | |
This is version 75 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Animal Toxin Annotation Program | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencingDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families