UniProtKB - Q1RMS5 (NXF1_BOVIN)
Nuclear RNA export factor 1
NXF1
Functioni
GO - Molecular functioni
- RNA binding Source: GO_Central
GO - Biological processi
- mRNA export from nucleus Source: UniProtKB
- poly(A)+ mRNA export from nucleus Source: GO_Central
Keywordsi
Molecular function | RNA-binding |
Biological process | mRNA transport, Transport |
Names & Taxonomyi
Protein namesi | Recommended name: Nuclear RNA export factor 1 |
Gene namesi | Name:NXF1 |
Organismi | Bos taurus (Bovine) |
Taxonomic identifieri | 9913 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Artiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos |
Proteomesi |
|
Organism-specific databases
VGNCi | VGNC:32375, NXF1 |
Subcellular locationi
Nucleus
- nucleoplasm By similarity
- Nucleus speckle By similarity
- Nucleus By similarity
Other locations
- Cytoplasm By similarity
Note: Localized predominantly in the nucleoplasm and at both the nucleoplasmic and cytoplasmic faces of the nuclear pore complex. Shuttles between the nucleus and the cytoplasm. Travels to the cytoplasm as part of the exon junction complex (EJC) bound to mRNA. The association with the TREX complex seems to occur in regions surrounding nuclear speckles known as perispeckles. Nucleus; nuclear rim.By similarity
Nucleus
- nuclear speck Source: UniProtKB
- nucleus Source: UniProtKB
Other locations
- cytoplasm Source: UniProtKB
Keywords - Cellular componenti
Cytoplasm, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedBy similarity | |||
ChainiPRO_0000270916 | 2 – 620 | Nuclear RNA export factor 1Add BLAST | 619 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylalanineBy similarity | 1 | |
Modified residuei | 41 | Asymmetric dimethylarginine; alternateBy similarity | 1 | |
Modified residuei | 41 | Omega-N-methylarginine; alternateBy similarity | 1 | |
Modified residuei | 127 | 3'-nitrotyrosineBy similarity | 1 |
Keywords - PTMi
Acetylation, Methylation, NitrationProteomic databases
PaxDbi | Q1RMS5 |
PRIDEi | Q1RMS5 |
Expressioni
Gene expression databases
Bgeei | ENSBTAG00000009488, Expressed in spleen and 18 other tissues |
ExpressionAtlasi | Q1RMS5, baseline |
Interactioni
Subunit structurei
Heterodimer (via NTF2 domain) with NXT1. The formation of NXF1-NXT1 heterodimers is required for the NXF1-mediated nuclear mRNA export.
Forms a complex with RANBP2/NUP358, NXT1 and RANGAP1. Associates with the exon junction complex (EJC) and with the transcription/export (TREX) complex.
Found in a mRNA complex with UPF3A and UPF3B.
Found in a post-splicing complex with RBM8A, UPF1, UPF2, UPF3A, UPF3B and RNPS1.
Interacts (via N-terminus) with DHX9 (via N-terminus); this interaction is direct and negatively regulates NXF1-mediated nuclear export of constitutive transport element (CTE)-containing cellular mRNAs.
Interacts with ALYREF/THOC4.
Interacts with FYTTD1/UIF.
Interacts with EIF4A3.
Interacts with NUPL2.
Interacts with THOC5.
Interacts with CHTOP.
Interacts with FRG1 (via N-terminus).
Interacts with LUZP4.
Interacts with FMR1; the interaction occurs in a mRNA-dependent and polyribosomes-independent manner in the nucleus.
Interacts with CPSF6 (via N-terminus); this interaction is direct.
Interacts with RBM15.
Interacts with RBM15B.
Interacts with MCM3AP; this interaction is not mediated by RNA (By similarity).
By similarityProtein-protein interaction databases
STRINGi | 9913.ENSBTAP00000012486 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 120 – 199 | RRMAdd BLAST | 80 | |
Repeati | 267 – 292 | LRR 1Add BLAST | 26 | |
Repeati | 293 – 316 | LRR 2Add BLAST | 24 | |
Repeati | 317 – 344 | LRR 3Add BLAST | 28 | |
Repeati | 345 – 372 | LRR 4Add BLAST | 28 | |
Domaini | 387 – 537 | NTF2PROSITE-ProRule annotationAdd BLAST | 151 | |
Domaini | 566 – 620 | TAP-CPROSITE-ProRule annotationAdd BLAST | 55 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 2 – 199 | Interaction with ALYREF/THOC4 and LUZP4By similarityAdd BLAST | 198 | |
Regioni | 2 – 119 | RNA-binding (RBD)By similarityAdd BLAST | 118 | |
Regioni | 2 – 59 | Minor non-specific RNA-bindingBy similarityAdd BLAST | 58 | |
Regioni | 60 – 119 | Major non-specific RNA-bindingBy similarityAdd BLAST | 60 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 66 – 101 | Nuclear localization signalBy similarityAdd BLAST | 36 | |
Motifi | 82 – 111 | Nuclear export signalBy similarityAdd BLAST | 30 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 552 – 562 | Pro-richAdd BLAST | 11 |
Domaini
Sequence similaritiesi
Keywords - Domaini
Leucine-rich repeat, RepeatPhylogenomic databases
eggNOGi | KOG3763, Eukaryota |
GeneTreei | ENSGT00390000007539 |
HOGENOMi | CLU_011280_2_0_1 |
InParanoidi | Q1RMS5 |
OMAi | IMDCHPL |
OrthoDBi | 1051093at2759 |
TreeFami | TF314566 |
Family and domain databases
CDDi | cd00780, NTF2, 1 hit cd14342, UBA_TAP-C, 1 hit |
Gene3Di | 3.30.70.330, 1 hit 3.80.10.10, 1 hit |
InterProi | View protein in InterPro IPR001611, Leu-rich_rpt IPR032675, LRR_dom_sf IPR002075, NTF2 IPR032710, NTF2-like_dom_sf IPR018222, Nuclear_transport_factor_2_euk IPR012677, Nucleotide-bd_a/b_plait_sf IPR030217, NXF_fam IPR035979, RBD_domain_sf IPR005637, TAP_C_dom IPR015245, Tap_RNA-bd IPR009060, UBA-like_sf |
PANTHERi | PTHR10662, PTHR10662, 1 hit |
Pfami | View protein in Pfam PF02136, NTF2, 1 hit PF09162, Tap-RNA_bind, 1 hit PF03943, TAP_C, 1 hit |
SMARTi | View protein in SMART SM00804, TAP_C, 1 hit |
SUPFAMi | SSF46934, SSF46934, 1 hit SSF54427, SSF54427, 1 hit SSF54928, SSF54928, 1 hit |
PROSITEi | View protein in PROSITE PS51450, LRR, 3 hits PS50177, NTF2_DOMAIN, 1 hit PS51281, TAP_C, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
10 20 30 40 50
MADEGKSYNE HDDRVSFPQR RKKGRGPFRW KSGEGNRRSG RGGSSVRSSR
60 70 80 90 100
LEDDDRDVAM SDVQDAPRVR YTPYAARPNR RGDNWNERTR IHVTLPLRRD
110 120 130 140 150
RSAAERGGAG TSQDGTSKNW FKITIPYGRK YDKSWLLNVI QSKCSVPFTP
160 170 180 190 200
IEFHYENTRA QFFVEDASTA SALKAVNYKI LDRENRRISI IINSSSPPHS
210 220 230 240 250
VQNELKPEQV EQLKLIMSKR YDGSQQALDL KGLRSDPDLV AQNIDVVLNR
260 270 280 290 300
RSCMAATLRI IEENIPELLS LNLSNNKLYR LDDLSSIVQK APNLKILNLS
310 320 330 340 350
GNELKSEREL DKIKGLKLEE LWLDGNTLCD TFRDQSTYIS AVRERFPKLL
360 370 380 390 400
RLDGHELPPP IAFDVEAPTT LPPCKGSYFG TETLKNLVLH FLQQYYAVYD
410 420 430 440 450
SGDRQRLLDA YHDGACCSLS IPFTPQNPAR SNLAEYFKDS RNVKKLKDPT
460 470 480 490 500
LRFRLLKHTR LNVVAFLNEL PKTQHDINSF VVDISAQTST LLCFSVNGVF
510 520 530 540 550
KEVDGKSRDS LRAFTRTFVA VPASNSGLCI VNDELFVRNA SADEIQRAFA
560 570 580 590 600
MPAPTPSSSP VPTLSPEQQE MLQAFSTQSG MNLEWSQKCL QDNNWDYTRS
610 620
AQAFTHLKAK GEIPEVAFMK
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A452DIA3 | A0A452DIA3_BOVIN | Nuclear RNA export factor 1 | NXF1 | 626 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC114739 mRNA Translation: AAI14740.1 |
RefSeqi | NP_001069023.1, NM_001075555.1 XP_015316721.1, XM_015461235.1 XP_015316722.1, XM_015461236.1 |
Genome annotation databases
Ensembli | ENSBTAT00000077484; ENSBTAP00000056842; ENSBTAG00000009488 |
GeneIDi | 512136 |
KEGGi | bta:512136 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC114739 mRNA Translation: AAI14740.1 |
RefSeqi | NP_001069023.1, NM_001075555.1 XP_015316721.1, XM_015461235.1 XP_015316722.1, XM_015461236.1 |
3D structure databases
BMRBi | Q1RMS5 |
SMRi | Q1RMS5 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 9913.ENSBTAP00000012486 |
Proteomic databases
PaxDbi | Q1RMS5 |
PRIDEi | Q1RMS5 |
Genome annotation databases
Ensembli | ENSBTAT00000077484; ENSBTAP00000056842; ENSBTAG00000009488 |
GeneIDi | 512136 |
KEGGi | bta:512136 |
Organism-specific databases
CTDi | 10482 |
VGNCi | VGNC:32375, NXF1 |
Phylogenomic databases
eggNOGi | KOG3763, Eukaryota |
GeneTreei | ENSGT00390000007539 |
HOGENOMi | CLU_011280_2_0_1 |
InParanoidi | Q1RMS5 |
OMAi | IMDCHPL |
OrthoDBi | 1051093at2759 |
TreeFami | TF314566 |
Gene expression databases
Bgeei | ENSBTAG00000009488, Expressed in spleen and 18 other tissues |
ExpressionAtlasi | Q1RMS5, baseline |
Family and domain databases
CDDi | cd00780, NTF2, 1 hit cd14342, UBA_TAP-C, 1 hit |
Gene3Di | 3.30.70.330, 1 hit 3.80.10.10, 1 hit |
InterProi | View protein in InterPro IPR001611, Leu-rich_rpt IPR032675, LRR_dom_sf IPR002075, NTF2 IPR032710, NTF2-like_dom_sf IPR018222, Nuclear_transport_factor_2_euk IPR012677, Nucleotide-bd_a/b_plait_sf IPR030217, NXF_fam IPR035979, RBD_domain_sf IPR005637, TAP_C_dom IPR015245, Tap_RNA-bd IPR009060, UBA-like_sf |
PANTHERi | PTHR10662, PTHR10662, 1 hit |
Pfami | View protein in Pfam PF02136, NTF2, 1 hit PF09162, Tap-RNA_bind, 1 hit PF03943, TAP_C, 1 hit |
SMARTi | View protein in SMART SM00804, TAP_C, 1 hit |
SUPFAMi | SSF46934, SSF46934, 1 hit SSF54427, SSF54427, 1 hit SSF54928, SSF54928, 1 hit |
PROSITEi | View protein in PROSITE PS51450, LRR, 3 hits PS50177, NTF2_DOMAIN, 1 hit PS51281, TAP_C, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | NXF1_BOVIN | |
Accessioni | Q1RMS5Primary (citable) accession number: Q1RMS5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 9, 2007 |
Last sequence update: | May 16, 2006 | |
Last modified: | February 10, 2021 | |
This is version 116 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families