UniProtKB - Q1PER6 (APX2_ARATH)
Protein
L-ascorbate peroxidase 2, cytosolic
Gene
APX2
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Functioni
Plays a key role in hydrogen peroxide removal.By similarity
Miscellaneous
Binds one cation per subunit; probably K+, but might also be Ca2+.By similarity
Catalytic activityi
- EC:1.11.1.11
Cofactori
heme bNote: Binds 1 heme b (iron(II)-protoporphyrin IX) group per subunit.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 39 | Transition state stabilizerPROSITE-ProRule annotation | 1 | |
Active sitei | 43 | Proton acceptorPROSITE-ProRule annotation | 1 | |
Metal bindingi | 163 | Iron (heme axial ligand)PROSITE-ProRule annotation | 1 | |
Metal bindingi | 164 | Potassium or calciumBy similarity | 1 | |
Metal bindingi | 180 | Potassium or calciumBy similarity | 1 | |
Metal bindingi | 182 | Potassium or calciumBy similarity | 1 | |
Metal bindingi | 185 | Potassium or calcium; via carbonyl oxygenBy similarity | 1 | |
Metal bindingi | 187 | Potassium or calciumBy similarity | 1 |
GO - Molecular functioni
- heme binding Source: InterPro
- L-ascorbate peroxidase activity Source: TAIR
- metal ion binding Source: UniProtKB-KW
- peroxidase activity Source: GO_Central
GO - Biological processi
- cellular response to oxidative stress Source: GO_Central
- hydrogen peroxide catabolic process Source: GO_Central
- response to oxidative stress Source: TAIR
- response to reactive oxygen species Source: GO_Central
Keywordsi
Molecular function | Oxidoreductase, Peroxidase |
Biological process | Hydrogen peroxide |
Ligand | Calcium, Heme, Iron, Metal-binding, Potassium |
Enzyme and pathway databases
BioCyci | ARA:AT3G09640-MONOMER |
Protein family/group databases
PeroxiBasei | 1888, AtAPx02 |
Names & Taxonomyi
Protein namesi | Recommended name: L-ascorbate peroxidase 2, cytosolic (EC:1.11.1.11)Alternative name(s): L-ascorbate peroxidase 1b Short name: APX1b Short name: AtAPx02 |
Gene namesi | Name:APX2 Synonyms:APX1B Ordered Locus Names:At3g09640 ORF Names:F11F8_23 |
Organismi | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic identifieri | 3702 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Proteomesi |
|
Organism-specific databases
Araporti | AT3G09640 |
TAIRi | locus:2074914, AT3G09640 |
Subcellular locationi
Other locations
- Cytoplasm By similarity
Chloroplast
- chloroplast Source: GO_Central
Cytosol
- cytosol Source: TAIR
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000261322 | 1 – 251 | L-ascorbate peroxidase 2, cytosolicAdd BLAST | 251 |
Proteomic databases
PaxDbi | Q1PER6 |
PRIDEi | Q1PER6 |
ProteomicsDBi | 246597 |
Expressioni
Tissue specificityi
Detected in bundle sheath cells, the photosynthetic cells that surround the phloem and xylem.1 Publication
Inductioni
By excess light treatment, by wounding and by heat-shock stress.4 Publications
Gene expression databases
ExpressionAtlasi | Q1PER6, baseline and differential |
Genevisiblei | Q1PER6, AT |
Family & Domainsi
Sequence similaritiesi
Phylogenomic databases
eggNOGi | ENOG502QR1E, Eukaryota |
HOGENOMi | CLU_036959_3_0_1 |
InParanoidi | Q1PER6 |
OMAi | HDANNGL |
OrthoDBi | 1228462at2759 |
PhylomeDBi | Q1PER6 |
Family and domain databases
InterProi | View protein in InterPro IPR002016, Haem_peroxidase IPR010255, Haem_peroxidase_sf IPR002207, Peroxidase_I IPR019794, Peroxidases_AS IPR019793, Peroxidases_heam-ligand_BS |
Pfami | View protein in Pfam PF00141, peroxidase, 1 hit |
PRINTSi | PR00459, ASPEROXIDASE PR00458, PEROXIDASE |
SUPFAMi | SSF48113, SSF48113, 1 hit |
PROSITEi | View protein in PROSITE PS00435, PEROXIDASE_1, 1 hit PS00436, PEROXIDASE_2, 1 hit PS50873, PEROXIDASE_4, 1 hit |
i Sequence
Sequence statusi: Complete.
Q1PER6-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MVKKSYPEVK EEYKKAVQRC KRKLRGLIAE KHCAPIVLRL AWHSAGTFDV
60 70 80 90 100
KTKTGGPFGT IRHPQELAHD ANNGLDIAVR LLDPIKELFP ILSYADFYQL
110 120 130 140 150
AGVVAVEITG GPEIPFHPGR LDKVEPPPEG RLPQATKGVD HLRDVFGRMG
160 170 180 190 200
LNDKDIVALS GGHTLGRCHK ERSGFEGAWT PNPLIFDNSY FKEILSGEKE
210 220 230 240 250
GLLQLPTDKA LLDDPLFLPF VEKYAADEDA FFEDYTEAHL KLSELGFADK
E
Sequence cautioni
The sequence AAF23294 differs from that shown. Reason: Erroneous gene model prediction.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 6 | Y → F in ABE65932 (PubMed:17147637).Curated | 1 | |
Sequence conflicti | 231 | F → S in CAA66925 (PubMed:8580771).Curated | 1 | |
Sequence conflicti | 231 | F → S in CAA56340 (PubMed:9144965).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X80036 Genomic DNA Translation: CAA56340.1 X98275 mRNA Translation: CAA66925.1 AC016661 Genomic DNA Translation: AAF23294.1 Sequence problems. CP002686 Genomic DNA Translation: AEE74791.1 CP002686 Genomic DNA Translation: AEE74792.1 AK176821 mRNA Translation: BAD44584.1 AK176908 mRNA Translation: BAD44671.1 DQ446651 mRNA Translation: ABE65932.1 |
RefSeqi | NP_001030664.1, NM_001035587.3 NP_187575.2, NM_111798.4 |
Genome annotation databases
EnsemblPlantsi | AT3G09640.1; AT3G09640.1; AT3G09640 AT3G09640.2; AT3G09640.2; AT3G09640 |
GeneIDi | 820121 |
Gramenei | AT3G09640.1; AT3G09640.1; AT3G09640 AT3G09640.2; AT3G09640.2; AT3G09640 |
KEGGi | ath:AT3G09640 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X80036 Genomic DNA Translation: CAA56340.1 X98275 mRNA Translation: CAA66925.1 AC016661 Genomic DNA Translation: AAF23294.1 Sequence problems. CP002686 Genomic DNA Translation: AEE74791.1 CP002686 Genomic DNA Translation: AEE74792.1 AK176821 mRNA Translation: BAD44584.1 AK176908 mRNA Translation: BAD44671.1 DQ446651 mRNA Translation: ABE65932.1 |
RefSeqi | NP_001030664.1, NM_001035587.3 NP_187575.2, NM_111798.4 |
3D structure databases
SMRi | Q1PER6 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 3702.AT3G09640.1 |
Protein family/group databases
PeroxiBasei | 1888, AtAPx02 |
Proteomic databases
PaxDbi | Q1PER6 |
PRIDEi | Q1PER6 |
ProteomicsDBi | 246597 |
Genome annotation databases
EnsemblPlantsi | AT3G09640.1; AT3G09640.1; AT3G09640 AT3G09640.2; AT3G09640.2; AT3G09640 |
GeneIDi | 820121 |
Gramenei | AT3G09640.1; AT3G09640.1; AT3G09640 AT3G09640.2; AT3G09640.2; AT3G09640 |
KEGGi | ath:AT3G09640 |
Organism-specific databases
Araporti | AT3G09640 |
TAIRi | locus:2074914, AT3G09640 |
Phylogenomic databases
eggNOGi | ENOG502QR1E, Eukaryota |
HOGENOMi | CLU_036959_3_0_1 |
InParanoidi | Q1PER6 |
OMAi | HDANNGL |
OrthoDBi | 1228462at2759 |
PhylomeDBi | Q1PER6 |
Enzyme and pathway databases
BioCyci | ARA:AT3G09640-MONOMER |
Miscellaneous databases
PROi | PR:Q1PER6 |
Gene expression databases
ExpressionAtlasi | Q1PER6, baseline and differential |
Genevisiblei | Q1PER6, AT |
Family and domain databases
InterProi | View protein in InterPro IPR002016, Haem_peroxidase IPR010255, Haem_peroxidase_sf IPR002207, Peroxidase_I IPR019794, Peroxidases_AS IPR019793, Peroxidases_heam-ligand_BS |
Pfami | View protein in Pfam PF00141, peroxidase, 1 hit |
PRINTSi | PR00459, ASPEROXIDASE PR00458, PEROXIDASE |
SUPFAMi | SSF48113, SSF48113, 1 hit |
PROSITEi | View protein in PROSITE PS00435, PEROXIDASE_1, 1 hit PS00436, PEROXIDASE_2, 1 hit PS50873, PEROXIDASE_4, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | APX2_ARATH | |
Accessioni | Q1PER6Primary (citable) accession number: Q1PER6 Secondary accession number(s): Q39006, Q67XB1, Q9SF39 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 28, 2006 |
Last sequence update: | January 23, 2007 | |
Last modified: | December 2, 2020 | |
This is version 99 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Arabidopsis thaliana
Arabidopsis thaliana: entries and gene names - SIMILARITY comments
Index of protein domains and families