UniProtKB - Q1PDC4 (L_MABVR)
RNA-directed RNA polymerase L
L
Functioni
RNA-directed RNA polymerase that catalyzes the transcription of viral mRNAs, their capping and polyadenylation. The template is composed of the viral RNA tightly encapsidated by the nucleoprotein (N). The viral polymerase binds to the genomic RNA at the 3' leader promoter, and transcribes subsequently all viral mRNAs with a decreasing efficiency. The first gene is the most transcribed, and the last the least transcribed. The viral phosphoprotein acts as a processivity factor. Capping is concommitant with initiation of mRNA transcription. Indeed, a GDP polyribonucleotidyl transferase (PRNTase) adds the cap structure when the nascent RNA chain length has reached few nucleotides. Ribose 2'-O methylation of viral mRNA cap precedes and facilitates subsequent guanine-N-7 methylation, both activities being carried by the viral polymerase. Polyadenylation of mRNAs occur by a stuttering mechanism at a slipery stop site present at the end viral genes. After finishing transcription of a mRNA, the polymerase can resume transcription of the downstream gene.
By similarityRNA-directed RNA polymerase that catalyzes the replication of viral genomic RNA. The template is composed of the viral RNA tightly encapsidated by the nucleoprotein (N). The replicase mode is dependent on intracellular N protein concentration. In this mode, the polymerase replicates the whole viral genome without recognizing transcriptional signals, and the replicated genome is not caped or polyadenylated.
By similarityCatalytic activityi
- EC:2.7.7.48PROSITE-ProRule annotation
- a 5'-end (5'-triphosphoguanosine)-adenylyl-adenylyl-cytidylyl-adenosine in mRNA + 2 S-adenosyl-L-methionine = a 5'-end (N7-methyl 5'-triphosphoguanosine)-(2'-O-methyladenylyl)-adenylyl-cytidylyl-adenosine in mRNA + H+ + 2 S-adenosyl-L-homocysteineBy similarityEC:2.1.1.375By similarity
- a 5'-end triphospho-adenylyl-adenylyl-cytidylyl-adenosine in mRNA + GDP + H+ = a 5'-end (5'-triphosphoguanosine)-adenylyl-adenylyl-cytidylyl-adenosine in mRNA + diphosphateBy similarityEC:2.7.7.88By similarity
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- GTPase activity Source: RHEA
- mRNA (guanine-N7-)-methyltransferase activity Source: InterPro
- RNA-directed 5'-3' RNA polymerase activity Source: UniProtKB-KW
GO - Biological processi
- negative stranded viral RNA replication Source: UniProtKB
- negative stranded viral RNA transcription Source: UniProtKB
Keywordsi
Molecular function | Hydrolase, Methyltransferase, Multifunctional enzyme, Nucleotidyltransferase, RNA-directed RNA polymerase, Transferase |
Biological process | mRNA capping, mRNA processing, Viral RNA replication |
Ligand | ATP-binding, Nucleotide-binding, S-adenosyl-L-methionine |
Names & Taxonomyi
Protein namesi | Recommended name: RNA-directed RNA polymerase LShort name: Protein L Alternative name(s): Large structural protein Replicase Transcriptase Including the following 4 domains: RNA-directed RNA polymerase (EC:2.7.7.48By similarity) Alternative name(s): PRNTaseCurated Alternative name(s): mRNA (guanine-N(7)-)-methyltransferaseBy similarity Short name: G-N7-MTaseBy similarity mRNA (nucleoside-2'-O-)-methyltransferaseBy similarity Short name: N1-2'-O-MTaseBy similarity |
Gene namesi | Name:L |
Organismi | Lake Victoria marburgvirus (strain Ravn-87) (MARV) (Marburg virus (strain Kenya/Ravn/1987)) |
Taxonomic identifieri | 378809 [NCBI] |
Taxonomic lineagei | Viruses › Riboviria › Orthornavirae › Negarnaviricota › Haploviricotina › Monjiviricetes › Mononegavirales › Filoviridae › Marburgvirus › |
Virus hosti | Chlorocebus aethiops (Green monkey) (Cercopithecus aethiops) [TaxID: 9534] Homo sapiens (Human) [TaxID: 9606] Rousettus aegyptiacus (Egyptian rousette) (Egyptian fruit bat) [TaxID: 9407] |
Proteomesi |
|
Subcellular locationi
- Host cytoplasm
- Virion By similarity
Keywords - Cellular componenti
Host cytoplasm, VirionPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000314972 | 1 – 2327 | RNA-directed RNA polymerase LAdd BLAST | 2327 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 628 – 812 | RdRp catalyticPROSITE-ProRule annotationAdd BLAST | 185 | |
Domaini | 1921 – 2118 | Mononegavirus-type SAM-dependent 2'-O-MTasePROSITE-ProRule annotationAdd BLAST | 198 |
Family and domain databases
InterProi | View protein in InterPro IPR039736, L_poly_C IPR026890, Mononeg_mRNAcap IPR014023, Mononeg_RNA_pol_cat IPR025786, Mononega_L_MeTrfase IPR017235, RNA-dir_pol_L_filovirus |
Pfami | View protein in Pfam PF14318, Mononeg_mRNAcap, 1 hit PF00946, Mononeg_RNA_pol, 1 hit |
PIRSFi | PIRSF037548, RNA_pol_Filoviridae, 1 hit |
TIGRFAMsi | TIGR04198, paramyx_RNAcap, 1 hit |
PROSITEi | View protein in PROSITE PS50526, RDRP_SSRNA_NEG_NONSEG, 1 hit PS51590, SAM_MT_MNV_L, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MQHPTQYPDA RLSSPIILDQ CDLLTRSLGL YSHYSHNPKL RNCRIPYHIY
60 70 80 90 100
RLRNSTALKT FLQNCSILTV PFHSIWDHII TSIQHDAINH INDFKYLLPS
110 120 130 140 150
ELIKYANWDN EFLRVFLNKI LRLDHAFTNS AKLQCEDFSP KENPYYWGML
160 170 180 190 200
LLVHLSQLAR RIKGQRGSLR SNWKFIGVDL ELFGIADFVI FKVPIKAIIR
210 220 230 240 250
NATSLQASKP GLKTWYRDQN LTPYLCDDEF VVSIASYECF IMIKDVFIER
260 270 280 290 300
YNTWEICARA WVEDNEEADY PPLGILRDLY NQGDQIITMY LEDGFKLIKH
310 320 330 340 350
LEPLCVSCIQ TYGIFTPRKY WFQSQMIKSY YDELQSLNLK LQIPDNRTEC
360 370 380 390 400
AQNFIKTIIQ AKLTPQQYCE LFSLQKHWGH PVLYNDVALD KVKKHAQSTK
410 420 430 440 450
ILKPKVMFET FCVFKFIVAK NHYHSQGSWY KTTHDLHLTP YLRQHIVSNS
460 470 480 490 500
FPSQAEIYQH LWEWYFVEHE PLFSTKIISD LSIFIKDRAT AVNRECWDSV
510 520 530 540 550
FDRSVLGYNP PVRFQSKRVP EQFLGQADFS LNQILDFAEK LEYLAPSYRN
560 570 580 590 600
FSFSLKEKEL NIGRTFGKLP YRVRNVQTLA EALLADGLAK AFPSNMMVVT
610 620 630 640 650
EREQKEALLH QASWHHNSAS IGENAIVRGA SFVTDLEKYN LAFRYEFTRH
660 670 680 690 700
FIDYCNRCYG VKNLFDWMHF LIPLCYMHVS DFYSPPHCVT ENNRNNPPDC
710 720 730 740 750
ANAYHYHLGG IEGLQQKLWT CISCAQITLV ELKTKLKLKS SVMGDNQCIT
760 770 780 790 800
TLSLFPIDAP NDYQENEAEL NAARVAVELA ITTGYSGIFL KPEETFVHSG
810 820 830 840 850
FIYFGKKQYL NGVQLPQSLK TMARCGPLSD SIFDDLQGSL ASIGTSFERG
860 870 880 890 900
ASETRHIFPS RWIAAFHSML AVNLLNQNHL GFPLGFSIDV SCFKKPLTFS
910 920 930 940 950
EKLIALITPQ VLGGLSFLNP EKLFYRNISD PLTSGLFQLR NALEFLRKEE
960 970 980 990 1000
LFYILIAKKP GLADASDFVM NPLGLNVPGS REIITFLRQT VRENITITSQ
1010 1020 1030 1040 1050
NRIINSLFHI GSDLEDQRVC EWLLSSNPVM SRFAADIFSR TPSGKRLQVL
1060 1070 1080 1090 1100
GYLEGTRTLL ASRTISLTTE GTMLMRLREL TKSRWKSWFS YIDALDDDLS
1110 1120 1130 1140 1150
ESLEKFICTV DVANFLRAYS WSDVLKGKRL IGATLPCLLE QFNVKWVNLS
1160 1170 1180 1190 1200
EDLKEQFKLS SDLGSPTDLL QYDCNGLHSK GADNAELNYV SCALDRKIVQ
1210 1220 1230 1240 1250
KHPSDNRLAW TIGNRAPYIG SRTEDKIGYP PLRVNCPSAA LKEAIEMVSR
1260 1270 1280 1290 1300
LLWVTQGTAD REKLLIPLLN SRVNLDYQTV LNFLPTHYSG NIVHRYNDQY
1310 1320 1330 1340 1350
GQHSFMANRM SNTSTRAIIS TNTLGKYAGG GQAAVDSNII FQNTINLGVA
1360 1370 1380 1390 1400
VLDITLSLSK LSSTSNVSFR LMLSKCCTRH VPSEYLFFDK PLDVDLNKYM
1410 1420 1430 1440 1450
DNELVYDNDP LCSGIKGRLG RVSRSTLSLS LNVSDIGSYD FPTIAAWTLG
1460 1470 1480 1490 1500
ETIIGSIFSD ESSQSTDPIS SGCTKTFVTH FLVYPVESIF YAFGANLIVE
1510 1520 1530 1540 1550
SLSLSRINSI KSLSDLTFLI SSTIRNLSHR SLRILQSTFR HELVLTRLAH
1560 1570 1580 1590 1600
HIPLISLMLG GSAGEKSSSD AVRLFLTASY QNFINNFSCL MRKNQSPLPV
1610 1620 1630 1640 1650
WLYFPSEGQQ LKPILKILQR LSCLLTTKKV QNHRPVADTC FLTDNFWVYP
1660 1670 1680 1690 1700
SKSTRTNHYY ASLNYWRDKA NKIKNTSFSH LINYSFSEPS LHASSISSSQ
1710 1720 1730 1740 1750
EVVNLKHTSR LDETPNMSER AQSTNHEPTA LQEVCTEIPY SEQDPAKSYL
1760 1770 1780 1790 1800
LLENTRFRDD QKILRHDQKA ERGEPLSLQV SSRGCLQALT CPHHPSPSQT
1810 1820 1830 1840 1850
TTEPLSMLRN CDAIKAALRS ETNDPRLMSS ILDMRSLKTP MRIESRNTSL
1860 1870 1880 1890 1900
LQPSECLSTS KGKSVLSREQ ASYLYVDCSN ISSISLDSGF RNMSDRNQVQ
1910 1920 1930 1940 1950
MLINTYKRDL YTCFDSNQFC RFTGVVSSMH YKLYDLLPAG KLGKAICLAE
1960 1970 1980 1990 2000
GEGSGARLLL KWKETDYLFF NTLATDSQQE AEILSGRVIP RMLYNIDKLS
2010 2020 2030 2040 2050
VLLESRKLIL NNLTIQITDI TNPLWLDSVI QYLPEDSDIL TMDAETTKEE
2060 2070 2080 2090 2100
TREQLYKTII NIWARTSPNI PKTSIIKVFL LDYGGTLFLM KNAIQYYGQV
2110 2120 2130 2140 2150
QLKKPYSSNA KNSEWYLCCG KRRVQRLRVD FPDQVGIFLI CKAMSRQRQA
2160 2170 2180 2190 2200
IPYWLKHIEK NYPASLHEFF ITLGFPSLES SFCHRYTIPF TEGTALFHKV
2210 2220 2230 2240 2250
QSYVRQGRQH LHSLMLDYEN NSPLLDLRNH FICSLRGKIT KYYNDILKLN
2260 2270 2280 2290 2300
LVVRAVERGK NWSQLVESLP NMHSVCITHV DHECIGCERR LLLKLDFVRN
2310 2320
TKIAEQKLLN RVIGYILFFP FGFSRPK
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | DQ447649 Genomic RNA Translation: ABE27074.1 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | DQ447649 Genomic RNA Translation: ABE27074.1 |
3D structure databases
SMRi | Q1PDC4 |
ModBasei | Search... |
Family and domain databases
InterProi | View protein in InterPro IPR039736, L_poly_C IPR026890, Mononeg_mRNAcap IPR014023, Mononeg_RNA_pol_cat IPR025786, Mononega_L_MeTrfase IPR017235, RNA-dir_pol_L_filovirus |
Pfami | View protein in Pfam PF14318, Mononeg_mRNAcap, 1 hit PF00946, Mononeg_RNA_pol, 1 hit |
PIRSFi | PIRSF037548, RNA_pol_Filoviridae, 1 hit |
TIGRFAMsi | TIGR04198, paramyx_RNAcap, 1 hit |
PROSITEi | View protein in PROSITE PS50526, RDRP_SSRNA_NEG_NONSEG, 1 hit PS51590, SAM_MT_MNV_L, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | L_MABVR | |
Accessioni | Q1PDC4Primary (citable) accession number: Q1PDC4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 15, 2008 |
Last sequence update: | May 16, 2006 | |
Last modified: | February 23, 2022 | |
This is version 78 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Viral Protein Annotation Program |