UniProtKB - Q1MX18 (INSC_HUMAN)
Protein inscuteable homolog
INSC
Functioni
May function as an adapter linking the Par3 complex to the GPSM1/GPSM2 complex (PubMed:16458856).
Involved in spindle orientation during mitosis. May regulate cell proliferation and differentiation in the developing nervous system. May play a role in the asymmetric division of fibroblasts and participate in the process of stratification of the squamous epithelium (By similarity).
By similarity1 PublicationGO - Molecular functioni
- cytoskeletal anchor activity Source: GO_Central
- protein domain specific binding Source: UniProtKB
- protein-macromolecule adaptor activity Source: UniProtKB
GO - Biological processi
- apical protein localization Source: GO_Central
- asymmetric cell division Source: InterPro
- cell differentiation Source: UniProtKB-KW
- establishment of mitotic spindle orientation Source: GO_Central
- nervous system development Source: UniProtKB-KW
- regulation of asymmetric cell division Source: GO_Central
- regulation of protein stability Source: UniProtKB
Keywordsi
Molecular function | Developmental protein |
Biological process | Differentiation, Neurogenesis |
Enzyme and pathway databases
PathwayCommonsi | Q1MX18 |
SignaLinki | Q1MX18 |
Names & Taxonomyi
Protein namesi | Recommended name: Protein inscuteable homolog |
Gene namesi | Name:INSC |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:33116, INSC |
MIMi | 610668, gene |
neXtProti | NX_Q1MX18 |
VEuPathDBi | HostDB:ENSG00000188487 |
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm 1 Publication
Other locations
- cell cortex 1 Publication
Note: Uniformly distributed in the cytoplasm during interphase. During metaphase, detected in the cell cortex, adjacent to the mitotic spindle poles.1 Publication
Plasma Membrane
- plasma membrane Source: UniProtKB
Other locations
- apical cortex Source: GO_Central
- protein-containing complex Source: UniProtKB
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 78 | W → A: Abolishes interaction with GPSM2. 1 Publication | 1 | |
Mutagenesisi | 89 | E → R: Strongly reduces interaction with GPSM2. Abolishes interaction with GPSM2 and GPSM1; when associated with D-97. 1 Publication | 1 | |
Mutagenesisi | 97 | K → D: Abolishes interaction with GPSM2 and GPSM1; when associated with R-89. 1 Publication | 1 |
Organism-specific databases
OpenTargetsi | ENSG00000188487 |
PharmGKBi | PA162392191 |
Miscellaneous databases
Pharosi | Q1MX18, Tbio |
Genetic variation databases
BioMutai | INSC |
DMDMi | 116248172 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000252405 | 1 – 579 | Protein inscuteable homologAdd BLAST | 579 |
Proteomic databases
EPDi | Q1MX18 |
MassIVEi | Q1MX18 |
PaxDbi | Q1MX18 |
PeptideAtlasi | Q1MX18 |
PRIDEi | Q1MX18 |
ProteomicsDBi | 61231 [Q1MX18-1] 61232 [Q1MX18-2] 61235 [Q1MX18-5] 69 |
PTM databases
iPTMneti | Q1MX18 |
PhosphoSitePlusi | Q1MX18 |
Expressioni
Tissue specificityi
Developmental stagei
Gene expression databases
Bgeei | ENSG00000188487, Expressed in tibia and 119 other tissues |
ExpressionAtlasi | Q1MX18, baseline and differential |
Genevisiblei | Q1MX18, HS |
Organism-specific databases
HPAi | ENSG00000188487, Tissue enriched (parathyroid) |
Interactioni
Subunit structurei
Binary interactionsi
Q1MX18
GO - Molecular functioni
- protein domain specific binding Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 132426, 37 interactors |
CORUMi | Q1MX18 |
IntActi | Q1MX18, 13 interactors |
STRINGi | 9606.ENSP00000368872 |
Miscellaneous databases
RNActi | Q1MX18, protein |
Structurei
Secondary structure
3D structure databases
AlphaFoldDBi | Q1MX18 |
SMRi | Q1MX18 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 74 – 89 | Important for interaction with GPSM21 PublicationAdd BLAST | 16 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 576 – 579 | PDZ-binding | 4 |
Phylogenomic databases
eggNOGi | ENOG502QRY2, Eukaryota |
GeneTreei | ENSGT00390000001511 |
InParanoidi | Q1MX18 |
OrthoDBi | 569133at2759 |
PhylomeDBi | Q1MX18 |
TreeFami | TF323559 |
Family and domain databases
Gene3Di | 1.25.10.10, 1 hit 6.20.200.10, 1 hit |
IDEALi | IID00323 |
InterProi | View protein in InterPro IPR011989, ARM-like IPR016024, ARM-type_fold IPR000225, Armadillo IPR045789, Insc_C IPR031938, INSC_LBD IPR038205, INSC_LBD_sf IPR039921, Inscuteable |
PANTHERi | PTHR21386, PTHR21386, 1 hit |
Pfami | View protein in Pfam PF19427, Insc_C, 1 hit PF16748, INSC_LBD, 1 hit |
SMARTi | View protein in SMART SM00185, ARM, 3 hits |
SUPFAMi | SSF48371, SSF48371, 1 hit |
s (6+)i Sequence
Sequence statusi: Complete.
This entry describes 6 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 6 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MRRPPGNGEA ASEGPGGWGL WGVQESRRLC CAGHDRCKQA LLQIGINMMA
60 70 80 90 100
LPGGRHLDSV TLPGQRLHLM QVDSVQRWME DLKLMTECEC MCVLQAKPIS
110 120 130 140 150
LEEDAQGDLI LAGGPGPGDP LQLLLKRGWV ISTELRRIGQ KLAQDRWARV
160 170 180 190 200
HSMSVRLTCH ARSMVSEYSA VSRNSLKEMG EIEKLLMEKC SELSAVTERC
210 220 230 240 250
LQVENEHVLK SMKACVSETL SMLGQHFGQL LELALTREVQ ALVRKIDASD
260 270 280 290 300
NIYTTESTTG NLFSLTQEGA PLCRIIAKEG GVVALFKVCR QDSFRCLYPQ
310 320 330 340 350
ALRTLASICC VEEGVHQLEK VDGVLCLADI LTDNSHSEAT RAEAAAVVAQ
360 370 380 390 400
VTSPHLPVTQ HLSSFLESME EIVTALVKLC QEASSGEVFL LASAALANIT
410 420 430 440 450
FFDTMACEML LQLNAIRVLL EACSDKQRVD TPYTRDQIVT ILANMSVLEQ
460 470 480 490 500
CASDIIQENG VQLIMGMLSE KPRSGTPAEV AACERVQQKA AVTLARLSRD
510 520 530 540 550
PDVAREAVRL SCMSRLIELC RSPSERNSSD AVLVACLAAL RRLAGVCPEG
560 570
LQDSDFQQLV QPRLVDSFLL CSNMEESFV
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A0A0MSI1 | A0A0A0MSI1_HUMAN | Protein inscuteable homolog | INSC | 566 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 212 | M → T in BAE17134 (Ref. 2) Curated | 1 | |
Sequence conflicti | 216 | V → M in BAE17135 (Ref. 2) Curated | 1 | |
Sequence conflicti | 226 | H → R in BAE17133 (Ref. 2) Curated | 1 | |
Sequence conflicti | 251 | N → D in BAE17134 (Ref. 2) Curated | 1 | |
Sequence conflicti | 513 | M → T in BAE17135 (Ref. 2) Curated | 1 | |
Sequence conflicti | 577 – 578 | SF → GS in BAE17136 (Ref. 2) Curated | 2 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_051073 | 333 | D → N. Corresponds to variant dbSNP:rs17507577Ensembl. | 1 | |
Natural variantiVAR_027852 | 450 | Q → R. Corresponds to variant dbSNP:rs7123855Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_020946 | 1 – 203 | Missing in isoform 3. 1 PublicationAdd BLAST | 203 | |
Alternative sequenceiVSP_020947 | 1 – 47 | Missing in isoform 2, isoform 4, isoform 5 and isoform 6. 3 PublicationsAdd BLAST | 47 | |
Alternative sequenceiVSP_020948 | 204 – 238 | ENEHV…ALTRE → MRREWEARMETHSPIWQNLL MRLDNCLALRLQSPL in isoform 3. 1 PublicationAdd BLAST | 35 | |
Alternative sequenceiVSP_020949 | 239 | V → VQVESHSVTRLECSGAISAQ CNLRLPGSSDSPASAS in isoform 5. 1 Publication | 1 | |
Alternative sequenceiVSP_020950 | 278 – 319 | Missing in isoform 3 and isoform 4. 1 PublicationAdd BLAST | 42 | |
Alternative sequenceiVSP_054159 | 539 – 579 | ALRRL…EESFV → MGIQLQVKKTNISALLLWFL FWHIQGCSA in isoform 6. 1 PublicationAdd BLAST | 41 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB236158 mRNA Translation: BAE93463.1 AB236159 mRNA Translation: BAE93464.1 AB231744 mRNA Translation: BAE46891.1 AB231745 mRNA Translation: BAE17133.1 AB231746 mRNA Translation: BAE17134.1 AB231747 mRNA Translation: BAE17135.1 AB231748 mRNA Translation: BAE17136.1 AC090744 Genomic DNA No translation available. BC127700 mRNA Translation: AAI27701.1 |
CCDSi | CCDS41621.1 [Q1MX18-1] CCDS41622.1 [Q1MX18-2] CCDS60735.1 [Q1MX18-6] CCDS60736.1 [Q1MX18-4] |
RefSeqi | NP_001027024.3, NM_001031853.4 [Q1MX18-1] NP_001036001.1, NM_001042536.2 [Q1MX18-2] NP_001265242.1, NM_001278313.1 [Q1MX18-2] NP_001265243.1, NM_001278314.1 NP_001265244.1, NM_001278315.1 [Q1MX18-6] NP_001265245.1, NM_001278316.1 [Q1MX18-4] XP_006718290.1, XM_006718227.3 [Q1MX18-2] XP_011518388.1, XM_011520086.1 [Q1MX18-6] XP_016873186.1, XM_017017697.1 [Q1MX18-2] |
Genome annotation databases
Ensembli | ENST00000379554.7; ENSP00000368872.3; ENSG00000188487.12 ENST00000379556.8; ENSP00000368874.3; ENSG00000188487.12 [Q1MX18-2] ENST00000525218.1; ENSP00000436113.1; ENSG00000188487.12 [Q1MX18-4] ENST00000528567.5; ENSP00000435022.1; ENSG00000188487.12 [Q1MX18-6] ENST00000530161.5; ENSP00000436194.1; ENSG00000188487.12 [Q1MX18-2] |
GeneIDi | 387755 |
KEGGi | hsa:387755 |
MANE-Selecti | ENST00000379556.8; ENSP00000368874.3; NM_001042536.3; NP_001036001.1 [Q1MX18-2] |
UCSCi | uc001mly.5, human [Q1MX18-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB236158 mRNA Translation: BAE93463.1 AB236159 mRNA Translation: BAE93464.1 AB231744 mRNA Translation: BAE46891.1 AB231745 mRNA Translation: BAE17133.1 AB231746 mRNA Translation: BAE17134.1 AB231747 mRNA Translation: BAE17135.1 AB231748 mRNA Translation: BAE17136.1 AC090744 Genomic DNA No translation available. BC127700 mRNA Translation: AAI27701.1 |
CCDSi | CCDS41621.1 [Q1MX18-1] CCDS41622.1 [Q1MX18-2] CCDS60735.1 [Q1MX18-6] CCDS60736.1 [Q1MX18-4] |
RefSeqi | NP_001027024.3, NM_001031853.4 [Q1MX18-1] NP_001036001.1, NM_001042536.2 [Q1MX18-2] NP_001265242.1, NM_001278313.1 [Q1MX18-2] NP_001265243.1, NM_001278314.1 NP_001265244.1, NM_001278315.1 [Q1MX18-6] NP_001265245.1, NM_001278316.1 [Q1MX18-4] XP_006718290.1, XM_006718227.3 [Q1MX18-2] XP_011518388.1, XM_011520086.1 [Q1MX18-6] XP_016873186.1, XM_017017697.1 [Q1MX18-2] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3SF4 | X-ray | 2.60 | D/E/F | 70-116 | [»] | |
AlphaFoldDBi | Q1MX18 | |||||
SMRi | Q1MX18 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 132426, 37 interactors |
CORUMi | Q1MX18 |
IntActi | Q1MX18, 13 interactors |
STRINGi | 9606.ENSP00000368872 |
PTM databases
iPTMneti | Q1MX18 |
PhosphoSitePlusi | Q1MX18 |
Genetic variation databases
BioMutai | INSC |
DMDMi | 116248172 |
Proteomic databases
EPDi | Q1MX18 |
MassIVEi | Q1MX18 |
PaxDbi | Q1MX18 |
PeptideAtlasi | Q1MX18 |
PRIDEi | Q1MX18 |
ProteomicsDBi | 61231 [Q1MX18-1] 61232 [Q1MX18-2] 61235 [Q1MX18-5] 69 |
Protocols and materials databases
Antibodypediai | 48935, 189 antibodies from 23 providers |
DNASUi | 387755 |
Genome annotation databases
Ensembli | ENST00000379554.7; ENSP00000368872.3; ENSG00000188487.12 ENST00000379556.8; ENSP00000368874.3; ENSG00000188487.12 [Q1MX18-2] ENST00000525218.1; ENSP00000436113.1; ENSG00000188487.12 [Q1MX18-4] ENST00000528567.5; ENSP00000435022.1; ENSG00000188487.12 [Q1MX18-6] ENST00000530161.5; ENSP00000436194.1; ENSG00000188487.12 [Q1MX18-2] |
GeneIDi | 387755 |
KEGGi | hsa:387755 |
MANE-Selecti | ENST00000379556.8; ENSP00000368874.3; NM_001042536.3; NP_001036001.1 [Q1MX18-2] |
UCSCi | uc001mly.5, human [Q1MX18-1] |
Organism-specific databases
CTDi | 387755 |
GeneCardsi | INSC |
HGNCi | HGNC:33116, INSC |
HPAi | ENSG00000188487, Tissue enriched (parathyroid) |
MIMi | 610668, gene |
neXtProti | NX_Q1MX18 |
OpenTargetsi | ENSG00000188487 |
PharmGKBi | PA162392191 |
VEuPathDBi | HostDB:ENSG00000188487 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | ENOG502QRY2, Eukaryota |
GeneTreei | ENSGT00390000001511 |
InParanoidi | Q1MX18 |
OrthoDBi | 569133at2759 |
PhylomeDBi | Q1MX18 |
TreeFami | TF323559 |
Enzyme and pathway databases
PathwayCommonsi | Q1MX18 |
SignaLinki | Q1MX18 |
Miscellaneous databases
BioGRID-ORCSi | 387755, 8 hits in 1070 CRISPR screens |
ChiTaRSi | INSC, human |
GenomeRNAii | 387755 |
Pharosi | Q1MX18, Tbio |
PROi | PR:Q1MX18 |
RNActi | Q1MX18, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000188487, Expressed in tibia and 119 other tissues |
ExpressionAtlasi | Q1MX18, baseline and differential |
Genevisiblei | Q1MX18, HS |
Family and domain databases
Gene3Di | 1.25.10.10, 1 hit 6.20.200.10, 1 hit |
IDEALi | IID00323 |
InterProi | View protein in InterPro IPR011989, ARM-like IPR016024, ARM-type_fold IPR000225, Armadillo IPR045789, Insc_C IPR031938, INSC_LBD IPR038205, INSC_LBD_sf IPR039921, Inscuteable |
PANTHERi | PTHR21386, PTHR21386, 1 hit |
Pfami | View protein in Pfam PF19427, Insc_C, 1 hit PF16748, INSC_LBD, 1 hit |
SMARTi | View protein in SMART SM00185, ARM, 3 hits |
SUPFAMi | SSF48371, SSF48371, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | INSC_HUMAN | |
Accessioni | Q1MX18Primary (citable) accession number: Q1MX18 Secondary accession number(s): A0PJX5 Q4AC98 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 17, 2006 |
Last sequence update: | May 30, 2006 | |
Last modified: | May 25, 2022 | |
This is version 123 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 11
Human chromosome 11: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references