SMAD1

Functionⁱ

Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD1 is a receptor-regulated SMAD (R-SMAD). May act synergistically with SMAD4 and YY1 in bone morphogenetic protein (BMP)-mediated cardiac-specific gene expression.By similarity

Sites

Feature key	Position(s)	DescriptionActions	Length
Metal bindingⁱ	64	ZincBy similarity	1
Metal bindingⁱ	109	ZincBy similarity	1
Metal bindingⁱ	121	ZincBy similarity	1
Metal bindingⁱ	126	ZincBy similarity	1

GO - Molecular functionⁱ

co-SMAD binding Source: Ensembl
DEAD/H-box RNA helicase binding Source: Ensembl
identical protein binding Source: Ensembl
I-SMAD binding Source: Ensembl
metal ion binding Source: UniProtKB-KW
primary miRNA binding Source: Ensembl
protein kinase binding Source: Ensembl
RNA polymerase II proximal promoter sequence-specific DNA binding Source: Ensembl
transcriptional activator activity, RNA polymerase II proximal promoter sequence-specific DNA binding Source: Ensembl

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

BMP signaling pathway Source: Ensembl
positive regulation of sprouting angiogenesis Source: Ensembl
SMAD protein complex assembly Source: Ensembl
transforming growth factor beta receptor signaling pathway Source: InterPro

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	DNA-binding
Biological process	Transcription, Transcription regulation
Ligand	Metal-binding, Zinc

Enzyme and pathway databases

Reactomeⁱ	R-BTA-201451 Signaling by BMP R-BTA-5689880 Ub-specific processing proteases R-BTA-8941326 RUNX2 regulates bone development

Reactomeⁱ

R-BTA-201451 Signaling by BMP
R-BTA-5689880 Ub-specific processing proteases
R-BTA-8941326 RUNX2 regulates bone development

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Mothers against decapentaplegic homolog 1 Short name: MAD homolog 1 Short name: Mothers against DPP homolog 1 Alternative name(s): SMAD family member 1 Short name: SMAD 1 Short name: Smad1
Gene namesⁱ	Name:SMAD1
Organismⁱ	Bos taurus (Bovine)
Taxonomic identifierⁱ	9913 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos
Proteomesⁱ	UP000009136 Componentⁱ: Chromosome 17

Organism-specific databases

VGNCⁱ	VGNC:34974 SMAD1

VGNCⁱ

VGNC:34974 SMAD1

Keywords - Cellular componentⁱ

Cytoplasm, Nucleus

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000260256	1 – 465	Mothers against decapentaplegic homolog 1Add BLAST		465

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Length
Modified residueⁱ	1	N-acetylmethionineBy similarity	1
Modified residueⁱ	322	Phosphothreonine; by MINK1, TNIK and MAP4K4By similarity	1
Modified residueⁱ	463	PhosphoserinePROSITE-ProRule annotationBy similarity	1
Modified residueⁱ	465	PhosphoserinePROSITE-ProRule annotationBy similarity	1

Post-translational modificationⁱ

Phosphorylation of the C-terminal SVS motif by BMP type 1 receptor kinase activates SMAD1 by promoting dissociation from the receptor and trimerization with SMAD4 (By similarity). Dephosphorylation, probably by PPM1A, induces its export from the nucleus to the cytoplasm (By similarity).By similarity

Ubiquitinated by SMAD-specific E3 ubiquitin ligase SMURF1, leading to its degradation. Monoubiquitinated, leading to prevent DNA-binding. Deubiquitination by USP15 alleviates inhibition and promotes activation of TGF-beta target genes (By similarity).By similarity

Keywords - PTMⁱ

Acetylation, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbⁱ	Q1JQA2
PRIDEⁱ	Q1JQA2

Expressionⁱ

Gene expression databases

Bgeeⁱ	ENSBTAG00000002835 Expressed in 10 organ(s), highest expression level in colon

Bgeeⁱ

ENSBTAG00000002835 Expressed in 10 organ(s), highest expression level in colon

Interactionⁱ

Subunit structureⁱ

Interacts with HGS, NANOG and ZCCHC12. Upon C-terminus phosphorylation: forms trimers with another SMAD1 and the co-SMAD SMAD4. Interacts with PEBP2-alpha subunit, CREB-binding protein (CBP), p300, SMURF1, SMURF2, USP15 and HOXC8. Associates with ZNF423 or ZNF521 in response to BMP2 leading to activate transcription of BMP target genes. Interacts with SKOR1. Interacts (via MH2 domain) with LEMD3. Binding to LEMD3 results in at least a partial reduction of receptor-mediated phosphorylation. Found in a complex with SMAD4 and YY1. Found in a macromolecular complex with FAM83G. Interacts (via MH2 domain) with FAM83G (via MH2 domain); in a SMAD4-independent manner. Interacts with ZC3H3. Interacts with TMEM119. Interacts (via MH1 and MH2 domains) with ZNF8 (By similarity). Interacts with RANBP3L; the interaction increases when SMAD1 is not phosphorylated and mediates SMAD1 nuclear export (By similarity).By similarity

GO - Molecular functionⁱ

co-SMAD binding Source: Ensembl
DEAD/H-box RNA helicase binding Source: Ensembl
identical protein binding Source: Ensembl
I-SMAD binding Source: Ensembl
protein kinase binding Source: Ensembl

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

STRINGⁱ	9913.ENSBTAP00000003668

STRINGⁱ

9913.ENSBTAP00000003668

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	Q1JQA2
SMRⁱ	Q1JQA2
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Domainⁱ	12 – 136	MH1PROSITE-ProRule annotationAdd BLAST		125
Domainⁱ	271 – 465	MH2PROSITE-ProRule annotationAdd BLAST		195

Region

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Regionⁱ	418 – 428	L3 loopBy similarityAdd BLAST		11

Compositional bias

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Compositional biasⁱ	39 – 45	Poly-Lys		7
Compositional biasⁱ	198 – 201	Poly-Ser		4

Domainⁱ

The MH2 domain mediates phosphorylation-dependent trimerization through L3 loop binding of phosphoserines in the adjacent subunit.By similarity

Sequence similaritiesⁱ

Belongs to the dwarfin/SMAD family.Curated

Phylogenomic databases

eggNOGⁱ	KOG3701 Eukaryota ENOG410XQKU LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00760000119091
HOGENOMⁱ	HOG000286019
HOVERGENⁱ	HBG053353
InParanoidⁱ	Q1JQA2
KEGG Orthology (KO) More...KOⁱ	K04676
OMAⁱ	GTPSKCV
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G082C
TreeFamⁱ	TF314923

Family and domain databases

Gene3Dⁱ	2.60.200.10, 1 hit 3.90.520.10, 1 hit
InterProⁱ	View protein in InterPro IPR013790 Dwarfin IPR003619 MAD_homology1_Dwarfin-type IPR013019 MAD_homology_MH1 IPR017855 SMAD-like_dom_sf IPR001132 SMAD_dom_Dwarfin-type IPR008984 SMAD_FHA_dom_sf IPR036578 SMAD_MH1_sf
PANTHERⁱ	PTHR13703 PTHR13703, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF03165 MH1, 1 hit PF03166 MH2, 1 hit
SMARTⁱ	View protein in SMART SM00523 DWA, 1 hit SM00524 DWB, 1 hit
SUPFAMⁱ	SSF49879 SSF49879, 1 hit SSF56366 SSF56366, 1 hit
PROSITEⁱ	View protein in PROSITE PS51075 MH1, 1 hit PS51076 MH2, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

Q1JQA2-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MNVTSLFSFT SPAVKRLLGW KQGDEEEKWA EKAVDALVKK LKKKKGAMEE 
        60         70         80         90        100
LEKALSCPGQ PSNCVTIPRS LDGRLQVSHR KGLPHVIYCR VWRWPDLQSH 
       110        120        130        140        150
HELKPLECCE FPFGSKQKEV CINPYHYKRV ESPVLPPVLV PRHSEYNPQH 
       160        170        180        190        200
SLLAQFRNLG QNEPHMPLNA TFPDSFQQPN SHPFPHSPNS SYPNSPGSSS 
       210        220        230        240        250
STYPHSPTSS DPGSPFQMPA DTPPPAYLPP EDPMTQDGSQ PMDTNMMAPS 
       260        270        280        290        300
LPSEINRGDV QAVAYEEPKH WCSIVYYELN NRVGEAFHAS STSVLVDGFT 
       310        320        330        340        350
DPSNNKNRFC LGLLSNVNRN STIENTRRHI GKGVHLYYVG GEVYAECLSD 
       360        370        380        390        400
SSIFVQSRNC NYHHGFHPTT VCKIPSGCSL KIFNNQEFAQ LLAQSVNHGF 
       410        420        430        440        450
ETVYELTKMC TIRMSFVKGW GAEYHRQDVT STPCWIEIHL HGPLQWLDKV 
       460 
LTQMGSPHNP ISSVS

Length:465

Mass (Da):52,250

Last modified:June 13, 2006 - v1

Checksum:ⁱ7C2E679F72B15C4F

GO

Experimental Info

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Sequence conflictⁱ	62	S → G in ABI96185 (Ref. 1) Curated		1

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	DQ922947 mRNA Translation: ABI96185.1 BC116117 mRNA Translation: AAI16118.1
NCBI Reference Sequences More...RefSeqⁱ	NP_001069691.2, NM_001076223.2 XP_005217566.1, XM_005217509.2 XP_005217568.1, XM_005217511.2 XP_010812017.1, XM_010813715.2 XP_010812018.1, XM_010813716.2
UniGeneⁱ	Bt.37151

Genome annotation databases

Ensemblⁱ	ENSBTAT00000003668; ENSBTAP00000003668; ENSBTAG00000002835
GeneIDⁱ	540488
KEGGⁱ	bta:540488

Similar proteinsⁱ

100% Identity
90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
Q1JQA2	Mothers against decapentaplegic homolog (Fragment)		wild yak		466	UniRef100_Q1JQA2
Mothers against decapentaplegic homolog		SHEEP		465
Mothers against decapentaplegic homolog		BUBBU		465
Mothers against decapentaplegic homolog		ICTTR		465

Protein	Similar proteins		Species	Score	Length	Source
Q1JQA2	Mothers against decapentaplegic homolog 1	)	HUMAN		465	UniRef90_Q15797
Mothers against decapentaplegic homolog 1		DANRE		472
Mothers against decapentaplegic homolog 1	)	MOUSE		465
Mothers against decapentaplegic homolog		MACMU		465
Mothers against decapentaplegic homolog		Cebus capucinus imitator		465
+132

Protein	Similar proteins		Species	Score	Length	Source
Q1JQA2	Mothers against decapentaplegic homolog 1	)	HUMAN		465	UniRef50_Q15797
Mothers against decapentaplegic homolog 1		DANRE		472
Mothers against decapentaplegic homolog 1	)	MOUSE		465
Mothers against decapentaplegic homolog 5		PONAB		465
Mothers against decapentaplegic homolog 5		CHICK		465
+515

Entry informationⁱ

Entry nameⁱ	SMAD1_BOVIN
Accessionⁱ	Q1JQA2Primary (citable) accession number: Q1JQA2 Secondary accession number(s): Q06AL6
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	November 28, 2006
	Last sequence update:	June 13, 2006
	Last modified:	September 12, 2018
	This is version 101 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

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UniParc

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UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - Q1JQA2 (SMAD1_BOVIN)

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Mothers against decapentaplegic homolog 1

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<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Sites

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Nucleus

Other locations

Nucleus

Other locations

Keywords - Cellular componenti

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Keywords - PTMi

Proteomic databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

GO - Molecular functioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Region

Compositional bias

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

Experimental Info

Sequence databases

Genome annotation databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Expressionⁱ

Interactionⁱ

GO - Molecular functionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ