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Protein

tRNA (cytosine(34)-C(5))-methyltransferase

Gene

Nsun2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

RNA methyltransferase that methylates tRNAs, and possibly RNA polymerase III transcripts. Methylates cytosine to 5-methylcytosine (m5C) at positions 34 and 48 of intron-containing tRNA(Leu)(CAA) precursors, and at positions 48, 49 and 50 of tRNA(Gly)(GCC) precursors (By similarity). May act downstream of Myc to regulate epidermal cell growth and proliferation. Required for proper spindle assembly and chromosome segregation, independently of its methyltransferase activity.By similarity2 Publications

Catalytic activityi

S-adenosyl-L-methionine + cytosine(34) in tRNA precursor = S-adenosyl-L-homocysteine + 5-methylcytosine(34) in tRNA precursor.

Activity regulationi

Inhibited by magnesium ions.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei215S-adenosyl-L-methioninePROSITE-ProRule annotation1
Binding sitei242S-adenosyl-L-methioninePROSITE-ProRule annotation1
Binding sitei268S-adenosyl-L-methioninePROSITE-ProRule annotation1
Active sitei321NucleophilePROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

  • cell division Source: UniProtKB-KW
  • hair follicle maturation Source: MGI
  • meiotic cell cycle checkpoint Source: CACAO
  • spermatid development Source: CACAO
  • tRNA methylation Source: MGI

Keywordsi

Molecular functionMethyltransferase, RNA-binding, Transferase, tRNA-binding
Biological processCell cycle, Cell division, Mitosis, tRNA processing
LigandS-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (cytosine(34)-C(5))-methyltransferase (EC:2.1.1.203)
Alternative name(s):
Myc-induced SUN domain-containing protein
Short name:
Misu
NOL1/NOP2/Sun domain family member 2
Gene namesi
Name:Nsun2
Synonyms:D13Wsu123e, Misu
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 13

Organism-specific databases

MGIiMGI:107252 Nsun2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002892241 – 757tRNA (cytosine(34)-C(5))-methyltransferaseAdd BLAST757

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei23PhosphoserineCombined sources1
Cross-linki46Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei139Phosphoserine; by AURKBBy similarity1
Modified residuei456PhosphoserineBy similarity1
Modified residuei473PhosphoserineBy similarity1
Cross-linki510Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki515Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei585N6-acetyllysine; alternateCombined sources1
Modified residuei585N6-malonyllysine; alternateBy similarity1
Cross-linki585Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity
Modified residuei592PhosphoserineBy similarity1
Cross-linki639Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki653Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki659Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei717PhosphothreonineCombined sources1
Modified residuei723PhosphoserineCombined sources1

Post-translational modificationi

Phosphorylated at Ser-139 by AURKB during mitosis, leading to abolish methyltransferase activity and the interaction with NPM1.By similarity

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ1HFZ0
MaxQBiQ1HFZ0
PaxDbiQ1HFZ0
PeptideAtlasiQ1HFZ0
PRIDEiQ1HFZ0

PTM databases

iPTMnetiQ1HFZ0
PhosphoSitePlusiQ1HFZ0
SwissPalmiQ1HFZ0

Expressioni

Tissue specificityi

Ubiquitously expressed at low level. Up-regulated in tumors.1 Publication

Developmental stagei

In G1, it is predominantly found in the nucleol. During S phase, it is present at its highest level and is distributed more uniformly throughout the nucleus (at protein level).1 Publication

Inductioni

By Myc (at protein level).1 Publication

Gene expression databases

BgeeiENSMUSG00000021595 Expressed in 318 organ(s), highest expression level in morula
CleanExiMM_NSUN2
ExpressionAtlasiQ1HFZ0 baseline and differential
GenevisibleiQ1HFZ0 MM

Interactioni

Subunit structurei

Interacts with NPM1 and NCL during interphase; interaction is disrupted following phosphorylation at Ser-139.By similarity

Protein-protein interaction databases

BioGridi205771, 5 interactors
IntActiQ1HFZ0, 1 interactor
STRINGi10090.ENSMUSP00000105321

Structurei

3D structure databases

ProteinModelPortaliQ1HFZ0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni184 – 190S-adenosyl-L-methionine bindingPROSITE-ProRule annotation7

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. RsmB/NOP family. TRM4 subfamily.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG2198 Eukaryota
COG0144 LUCA
GeneTreeiENSGT00660000095589
HOGENOMiHOG000205147
HOVERGENiHBG106711
InParanoidiQ1HFZ0
KOiK15335
OMAiTFVRCEN
OrthoDBiEOG091G04FO
PhylomeDBiQ1HFZ0
TreeFamiTF300702

Family and domain databases

InterProiView protein in InterPro
IPR001678 MeTrfase_RsmB/NOP2
IPR023267 RCMT
IPR023270 RCMT_NCL1
IPR029063 SAM-dependent_MTases
PfamiView protein in Pfam
PF01189 Methyltr_RsmB-F, 1 hit
PRINTSiPR02008 RCMTFAMILY
PR02011 RCMTNCL1
SUPFAMiSSF53335 SSF53335, 2 hits
PROSITEiView protein in PROSITE
PS51686 SAM_MT_RSMB_NOP, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q1HFZ0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MGRRARGRRF QQPPQPEGEE DASDGGRKRG QAGWEGGYPE IVKENKLFEH
60 70 80 90 100
YYQELKIVPE GEWDQFMESL REPLPATLRI TGYKSHAKEI LHCLKNKYFK
110 120 130 140 150
ELEDLEVDGQ KVEVPQPLSW YPEELAWHTN LSRKILRKSP LLAKFHQFLV
160 170 180 190 200
SETESGNISR QEAVSMIPPL LLNVEPHHKI LDMCAAPGSK TTQLIEMLHA
210 220 230 240 250
DMSVPFPEGF VIANDVDNKR CYLLVHQAKR LSSPCIMVVN HDASSIPRLT
260 270 280 290 300
VDVDGRKEIL FYDRILCDVP CSGDGTMRKN IDVWKKWTTL NSLQLHGLQL
310 320 330 340 350
RIATRGAEQL AEGGRMVYST CSLNPVEDEA VIAALLEKSE GALELADVSA
360 370 380 390 400
ELPGLKWMPG VSQWKVMTRD GQWFADWHEV PQGRHTQIRP TMFPPTDLEK
410 420 430 440 450
LQAMHLERCL RILPHHQNTG GFFVAVLVKK APMPWNKRQP KVQNKSAEAR
460 470 480 490 500
EPRVSSHVAA TEGNPSDQSE LESQMITGAG DSETAHNTEN TESNEKKDGV
510 520 530 540 550
CGPPPSKKMK LFGFKEDPFV FIPEDDPLFP PIEKFYALDP SFPRMNLLTR
560 570 580 590 600
TTEGKKRQLY MVSKELRNVL LNNSEKMKVI NTGIKVWCRN NSGEEFDCAF
610 620 630 640 650
RLAQEGIYTL YPFINSRIIT VSMEDVKTLL TQENPFFRKL SSEAYSQVKD
660 670 680 690 700
LAKGSVVLKY EPDSANPDTL QCPIVLCGWR GKASIRTFVP KNERLHYLRM
710 720 730 740 750
MGLEVLGEKK KEGVILTNEN AASPEQPGDE DAKQTAQDPC VPDSVPGCDA

AAAEPSR
Length:757
Mass (Da):85,452
Last modified:May 29, 2007 - v2
Checksum:i73B06947DEC50298
GO
Isoform 2 (identifier: Q1HFZ0-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-66: Missing.

Note: No experimental confirmation available.
Show »
Length:691
Mass (Da):77,747
Checksum:iCA8B554730D496E6
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
H3BKN0H3BKN0_MOUSE
tRNA (cytosine(34)-C(5))-methyltran...
Nsun2
722Annotation score:
H3BJF3H3BJF3_MOUSE
tRNA (cytosine(34)-C(5))-methyltran...
Nsun2
72Annotation score:

Sequence cautioni

The sequence AAH13625 differs from that shown. Reason: Erroneous initiation.Curated
The sequence BAC36110 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti206F → L in BAE30795 (PubMed:16141072).Curated1
Sequence conflicti206F → L in BAE29720 (PubMed:16141072).Curated1
Sequence conflicti253V → L in BAE30795 (PubMed:16141072).Curated1
Sequence conflicti253V → L in BAE29720 (PubMed:16141072).Curated1
Sequence conflicti482S → L in ABF29536 (PubMed:16713953).Curated1
Sequence conflicti482S → L in AAH25549 (PubMed:15489334).Curated1
Sequence conflicti490N → I in ABF29536 (PubMed:16713953).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0259691 – 66Missing in isoform 2. 1 PublicationAdd BLAST66

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ490066 mRNA Translation: ABF29536.1
AK030124 mRNA Translation: BAC26795.1
AK075999 mRNA Translation: BAC36110.1 Different initiation.
AK150631 mRNA Translation: BAE29720.1
AK151917 mRNA Translation: BAE30795.1
BC013625 mRNA Translation: AAH13625.1 Different initiation.
BC025549 mRNA Translation: AAH25549.1 Different termination.
CCDSiCCDS36722.2 [Q1HFZ0-1]
RefSeqiNP_663329.3, NM_145354.5 [Q1HFZ0-1]
UniGeneiMm.260009

Genome annotation databases

EnsembliENSMUST00000022087; ENSMUSP00000022087; ENSMUSG00000021595 [Q1HFZ0-2]
ENSMUST00000109699; ENSMUSP00000105321; ENSMUSG00000021595 [Q1HFZ0-1]
GeneIDi28114
KEGGimmu:28114
UCSCiuc007rcm.3 mouse [Q1HFZ0-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ490066 mRNA Translation: ABF29536.1
AK030124 mRNA Translation: BAC26795.1
AK075999 mRNA Translation: BAC36110.1 Different initiation.
AK150631 mRNA Translation: BAE29720.1
AK151917 mRNA Translation: BAE30795.1
BC013625 mRNA Translation: AAH13625.1 Different initiation.
BC025549 mRNA Translation: AAH25549.1 Different termination.
CCDSiCCDS36722.2 [Q1HFZ0-1]
RefSeqiNP_663329.3, NM_145354.5 [Q1HFZ0-1]
UniGeneiMm.260009

3D structure databases

ProteinModelPortaliQ1HFZ0
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi205771, 5 interactors
IntActiQ1HFZ0, 1 interactor
STRINGi10090.ENSMUSP00000105321

PTM databases

iPTMnetiQ1HFZ0
PhosphoSitePlusiQ1HFZ0
SwissPalmiQ1HFZ0

Proteomic databases

EPDiQ1HFZ0
MaxQBiQ1HFZ0
PaxDbiQ1HFZ0
PeptideAtlasiQ1HFZ0
PRIDEiQ1HFZ0

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000022087; ENSMUSP00000022087; ENSMUSG00000021595 [Q1HFZ0-2]
ENSMUST00000109699; ENSMUSP00000105321; ENSMUSG00000021595 [Q1HFZ0-1]
GeneIDi28114
KEGGimmu:28114
UCSCiuc007rcm.3 mouse [Q1HFZ0-1]

Organism-specific databases

CTDi54888
MGIiMGI:107252 Nsun2

Phylogenomic databases

eggNOGiKOG2198 Eukaryota
COG0144 LUCA
GeneTreeiENSGT00660000095589
HOGENOMiHOG000205147
HOVERGENiHBG106711
InParanoidiQ1HFZ0
KOiK15335
OMAiTFVRCEN
OrthoDBiEOG091G04FO
PhylomeDBiQ1HFZ0
TreeFamiTF300702

Miscellaneous databases

ChiTaRSiNsun2 mouse
PROiPR:Q1HFZ0
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000021595 Expressed in 318 organ(s), highest expression level in morula
CleanExiMM_NSUN2
ExpressionAtlasiQ1HFZ0 baseline and differential
GenevisibleiQ1HFZ0 MM

Family and domain databases

InterProiView protein in InterPro
IPR001678 MeTrfase_RsmB/NOP2
IPR023267 RCMT
IPR023270 RCMT_NCL1
IPR029063 SAM-dependent_MTases
PfamiView protein in Pfam
PF01189 Methyltr_RsmB-F, 1 hit
PRINTSiPR02008 RCMTFAMILY
PR02011 RCMTNCL1
SUPFAMiSSF53335 SSF53335, 2 hits
PROSITEiView protein in PROSITE
PS51686 SAM_MT_RSMB_NOP, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiNSUN2_MOUSE
AccessioniPrimary (citable) accession number: Q1HFZ0
Secondary accession number(s): A0PJD6
, Q3U972, Q8BPG9, Q8CDF9, Q91YX9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: May 29, 2007
Last modified: October 10, 2018
This is version 108 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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