UniProtKB - Q1ENI8 (PXDN1_CAEEL)
Peroxidasin homolog pxn-1
pxn-1
Functioni
Catalyzes the two-electron oxidation of bromide by hydrogen peroxide and generates hypobromite as a reactive intermediate which mediates the formation of sulfilimine cross-links between methionine and hydroxylysine residues within an uncross-linked collagen IV NC1 hexamer (By similarity).
Plays a role in the attachment of tissues and in axonal guidance during early developmental stages (PubMed:25475546).
May functionally antagonize the peroxidasin pxn-2 to maintain neuronal development (PubMed:20876652, PubMed:26194821).
By similarity3 PublicationsCatalytic activityi
- H2O2 + L-lysyl-[collagen] + L-methionyl-[collagen] = [collagen]-L-lysyl-N-S-L-methionyl-[collagen] + H+ + 2 H2OBy similarityThis reaction proceeds in the forwardBy similarity direction.
- This reaction proceeds in the forwardBy similarity direction.
- hypobromite + L-lysyl-[collagen] + L-methionyl-[collagen] = [collagen]-L-lysyl-N-S-L-methionyl-[collagen] + bromide + H+ + H2OBy similarityThis reaction proceeds in the forwardBy similarity direction.
- This reaction proceeds in the forwardBy similarity direction.
- This reaction proceeds in the forwardBy similarity direction.
Cofactori
Protein has several cofactor binding sites:- Ca2+PROSITE-ProRule annotationNote: Binds 1 Ca2+ ion per subunit.PROSITE-ProRule annotation
- heme bPROSITE-ProRule annotationNote: Binds 1 heme b (iron(II)-protoporphyrin IX) group covalently per subunit.PROSITE-ProRule annotation
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 719 | Heme b; covalent, via 2 linksPROSITE-ProRule annotation | 1 | |
Active sitei | 720 | Proton acceptorPROSITE-ProRule annotation | 1 | |
Metal bindingi | 721 | CalciumPROSITE-ProRule annotation | 1 | |
Metal bindingi | 803 | CalciumPROSITE-ProRule annotation | 1 | |
Metal bindingi | 805 | Calcium; via carbonyl oxygenPROSITE-ProRule annotation | 1 | |
Metal bindingi | 807 | CalciumPROSITE-ProRule annotation | 1 | |
Metal bindingi | 809 | CalciumPROSITE-ProRule annotation | 1 | |
Sitei | 874 | Transition state stabilizerPROSITE-ProRule annotation | 1 | |
Binding sitei | 877 | Heme b; covalent, via 2 linksPROSITE-ProRule annotation | 1 | |
Metal bindingi | 973 | Iron (heme b axial ligand)PROSITE-ProRule annotation | 1 |
GO - Molecular functioni
- heme binding Source: InterPro
- metal ion binding Source: UniProtKB-KW
- peroxidase activity Source: GO_Central
GO - Biological processi
- embryonic body morphogenesis Source: InterPro
- hydrogen peroxide catabolic process Source: UniProtKB-KW
- muscle attachment Source: UniProtKB
- positive regulation of axon guidance Source: UniProtKB
- regulation of axon regeneration Source: InterPro
- response to oxidative stress Source: InterPro
Keywordsi
Molecular function | Oxidoreductase, Peroxidase |
Biological process | Hydrogen peroxide |
Ligand | Calcium, Heme, Iron, Metal-binding |
Enzyme and pathway databases
Reactomei | R-CEL-209968, Thyroxine biosynthesis R-CEL-2243919, Crosslinking of collagen fibrils R-CEL-6798695, Neutrophil degranulation R-CEL-8941413, Events associated with phagocytolytic activity of PMN cells |
Protein family/group databases
PeroxiBasei | 3359, CelPxd01 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:pxn-1Imported ORF Names:ZK994.3Imported |
Organismi | Caenorhabditis elegans |
Taxonomic identifieri | 6239 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Ecdysozoa › Nematoda › Chromadorea › Rhabditida › Rhabditina › Rhabditomorpha › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis |
Proteomesi |
|
Organism-specific databases
WormBasei | ZK994.3 ; CE40298 ; WBGene00004256 ; pxn-1 |
Subcellular locationi
Extracellular region or secreted
- extracellular matrix 1 Publication
Note: Localizes to the extracellular space in between body wall muscle cells.1 Publication
Extracellular region or secreted
- extracellular space Source: GO_Central
Other locations
- basement membrane Source: WormBase
- extracellular matrix Source: UniProtKB
Keywords - Cellular componenti
Extracellular matrix, SecretedPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 20 | Sequence analysisAdd BLAST | 20 | |
ChainiPRO_0000319623 | 21 – 1285 | Peroxidasin homolog pxn-1Add BLAST | 1265 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 49 | N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation | 1 | |
Glycosylationi | 248 | N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation | 1 | |
Disulfide bondi | 336 ↔ 385 | PROSITE-ProRule annotation | ||
Disulfide bondi | 429 ↔ 479 | PROSITE-ProRule annotation | ||
Glycosylationi | 595 | N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation | 1 | |
Disulfide bondi | 625 ↔ 641 | PROSITE-ProRule annotation | ||
Disulfide bondi | 740 ↔ 750 | PROSITE-ProRule annotation | ||
Glycosylationi | 741 | N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation1 Publication | 1 | |
Disulfide bondi | 744 ↔ 771 | PROSITE-ProRule annotation | ||
Glycosylationi | 858 | N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation1 Publication | 1 | |
Disulfide bondi | 1076 ↔ 1133 | PROSITE-ProRule annotation | ||
Disulfide bondi | 1174 ↔ 1201 | PROSITE-ProRule annotation |
Keywords - PTMi
Disulfide bond, GlycoproteinProteomic databases
EPDi | Q1ENI8 |
PaxDbi | Q1ENI8 |
PeptideAtlasi | Q1ENI8 |
PTM databases
iPTMneti | Q1ENI8 |
Expressioni
Tissue specificityi
Developmental stagei
Gene expression databases
Bgeei | WBGene00004256, Expressed in material anatomical entity and 4 other tissues |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 21 – 53 | LRRNTAdd BLAST | 33 | |
Repeati | 27 – 49 | LRR 1Sequence analysisAdd BLAST | 23 | |
Repeati | 50 – 72 | LRR 2Sequence analysisAdd BLAST | 23 | |
Repeati | 73 – 96 | LRR 3Sequence analysisAdd BLAST | 24 | |
Repeati | 97 – 120 | LRR 4Sequence analysisAdd BLAST | 24 | |
Repeati | 122 – 143 | LRR 5Sequence analysisAdd BLAST | 22 | |
Repeati | 145 – 168 | LRR 6Sequence analysisAdd BLAST | 24 | |
Domaini | 180 – 228 | LRRCTSequence analysisAdd BLAST | 49 | |
Repeati | 204 – 227 | LRR 7Sequence analysisAdd BLAST | 24 | |
Domaini | 315 – 401 | Ig-like C2-type 1PROSITE-ProRule annotationAdd BLAST | 87 | |
Domaini | 408 – 495 | Ig-like C2-type 2PROSITE-ProRule annotationAdd BLAST | 88 | |
Repeati | 998 – 1022 | LRR 10Sequence analysisAdd BLAST | 25 | |
Repeati | 1049 – 1073 | LRR 11Sequence analysisAdd BLAST | 25 | |
Repeati | 1168 – 1189 | LRR 12Sequence analysisAdd BLAST | 22 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 305 – 324 | DisorderedSequence analysisAdd BLAST | 20 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 508 – 550 | Sequence analysisAdd BLAST | 43 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 308 – 323 | Polar residuesSequence analysisAdd BLAST | 16 |
Sequence similaritiesi
Keywords - Domaini
Coiled coil, Immunoglobulin domain, Leucine-rich repeat, Repeat, SignalPhylogenomic databases
eggNOGi | KOG0619, Eukaryota KOG2408, Eukaryota |
GeneTreei | ENSGT00940000168557 |
HOGENOMi | CLU_006087_0_0_1 |
InParanoidi | Q1ENI8 |
OMAi | FHRKYRA |
OrthoDBi | 276568at2759 |
PhylomeDBi | Q1ENI8 |
Family and domain databases
CDDi | cd09826, peroxidasin_like, 1 hit |
Gene3Di | 1.10.640.10, 1 hit 2.60.40.10, 2 hits 3.80.10.10, 2 hits |
InterProi | View protein in InterPro IPR000483, Cys-rich_flank_reg_C IPR019791, Haem_peroxidase_animal IPR010255, Haem_peroxidase_sf IPR037120, Haem_peroxidase_sf_animal IPR007110, Ig-like_dom IPR036179, Ig-like_dom_sf IPR013783, Ig-like_fold IPR013098, Ig_I-set IPR003599, Ig_sub IPR003598, Ig_sub2 IPR001611, Leu-rich_rpt IPR003591, Leu-rich_rpt_typical-subtyp IPR032675, LRR_dom_sf IPR000372, LRRNT IPR034824, Peroxidasin_peroxidase IPR034826, Peroxidasin_PXN-1_nema |
PANTHERi | PTHR11475:SF74, PTHR11475:SF74, 1 hit |
Pfami | View protein in Pfam PF03098, An_peroxidase, 1 hit PF07679, I-set, 2 hits PF13855, LRR_8, 2 hits PF01462, LRRNT, 1 hit |
PRINTSi | PR00457, ANPEROXIDASE |
SMARTi | View protein in SMART SM00409, IG, 2 hits SM00408, IGc2, 2 hits SM00369, LRR_TYP, 3 hits SM00082, LRRCT, 1 hit SM00013, LRRNT, 1 hit |
SUPFAMi | SSF48113, SSF48113, 1 hit SSF48726, SSF48726, 2 hits |
PROSITEi | View protein in PROSITE PS50835, IG_LIKE, 2 hits PS50292, PEROXIDASE_3, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
10 20 30 40 50
MNLYLLLLVI ATSSWQFVAG LECPVECTCD KKGLVVDCSS SGLTRIPKNI
60 70 80 90 100
SRNVRSLVIR NNRIHKLKRS DLEGFNQLET LVLTHNKIKI IEENVLDHLP
110 120 130 140 150
ELKRLSLAHN ELVYIPPLCS DSRPLASLNL KRNHIQFIDE QVLRYFPDLT
160 170 180 190 200
QLDFSHNRIQ SLRTKLFDNL PALSHAHLHS NPWHCDCRAS KVKALLQKVK
210 220 230 240 250
WEKKVYCTNP VELRHQALDE VDDSALTCAR PAEESWTGEE IKLTCAKNSS
260 270 280 290 300
SKLVVWMYEN VEVDSSSLDG YEIHDTVITV PRKTNVNFMT CTYDFDHIPH
310 320 330 340 350
HRRLRQSQHQ GNGSPQFTYK PRDNSFREGS EVKVNCEVMG NPKPTINWYH
360 370 380 390 400
NGKRFISSRK RQLGLSNNVL RIYPFLEEDS GRYTCEAVNS VGKVRHAFSL
410 420 430 440 450
DLISSVPPNI YEGPQSVSQN LGGSVVFVCK ANGNPVPDYT WSFDGSTIGH
460 470 480 490 500
IKGRFMVSDD GTELRISNIE KKDEGYYSCM AGNPVGAMSA DAKLTVIGGE
510 520 530 540 550
TRKAAAPQID EELLRAIAQK ARQNVENAVE KTRKQLTQDK VTNTNDLKRL
560 570 580 590 600
FRFSTPKQAV ELSKAREIYE ESVRLVREHV EKGLILNVDE LHPKNVSYES
610 620 630 640 650
VLHVTHVQAL MGLSGCHTGQ YKNPCTDTCF HHRYRSFDGQ CNNKNKPMTG
660 670 680 690 700
VSLMPLRRLL KPVYENGFNT PVGWEKGRLY NGYPLPNVRE VSRQLVATEN
710 720 730 740 750
ITPHSKLSSM VMQWGQFVDH DLTHTVTALS RHSYATGAFC NRTCENLDPC
760 770 780 790 800
FNIPLSPNDP RVKSGSAKYP CIEFERSAAV CGSGETSLVF NRVTYREQMN
810 820 830 840 850
ALTSFLDASN VYGSNEVQAQ ELRDTYNNNG MLRFDITSEA GKEYLPFEKD
860 870 880 890 900
SNMDCRRNFS EENPIRCFLA GDLRANEQLA LAATHTIFIR EHNRIAKKLK
910 920 930 940 950
SMNGNWDGEI IYHETRKIVG AMMQHITYKH WMPIIFGGQA QMNKFVGTYQ
960 970 980 990 1000
GYDPDVDASV TNAFATAAFR FGHTIINPSL FRLGNDFMPI KEGHIALHKA
1010 1020 1030 1040 1050
FFTPELVLTQ GGVDPLLRGL FASPLKHPMP TQLLNMELIE KLFMKGHEVS
1060 1070 1080 1090 1100
LDLAVMNIQR SRDHGLPSYT EYRKFCNLPV PVQWEDMKGY IKDDMIIQKL
1110 1120 1130 1140 1150
RGLYGVPQNI DLWVGGIVEE KLENGLFGPT FACIIGEQFR KIRDGDRFWY
1160 1170 1180 1190 1200
EKDGVFTPEQ LREIKKITLA RLFCDNGDNI DRIQKDVFMY PGMDKENYGT
1210 1220 1230 1240 1250
CQETEMMNLR AWSKCCDNVC PTMLDRILRS RHRGSRLHGC NQNGIWRPEG
1260 1270 1280
AKWIPQNEIC TECVCQGSRV WCSTKEDCSD NRSPF
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BX284605 Genomic DNA Translation: CCD71290.1 |
RefSeqi | NP_505188.3, NM_072787.3 |
Genome annotation databases
EnsemblMetazoai | ZK994.3.1; ZK994.3.1; WBGene00004256 |
GeneIDi | 191484 |
KEGGi | cel:CELE_ZK994.3 |
UCSCi | ZK994.3, c. elegans |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BX284605 Genomic DNA Translation: CCD71290.1 |
RefSeqi | NP_505188.3, NM_072787.3 |
3D structure databases
AlphaFoldDBi | Q1ENI8 |
SMRi | Q1ENI8 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 56017, 3 interactors |
STRINGi | 6239.ZK994.3 |
Protein family/group databases
PeroxiBasei | 3359, CelPxd01 |
PTM databases
iPTMneti | Q1ENI8 |
Proteomic databases
EPDi | Q1ENI8 |
PaxDbi | Q1ENI8 |
PeptideAtlasi | Q1ENI8 |
Genome annotation databases
EnsemblMetazoai | ZK994.3.1; ZK994.3.1; WBGene00004256 |
GeneIDi | 191484 |
KEGGi | cel:CELE_ZK994.3 |
UCSCi | ZK994.3, c. elegans |
Organism-specific databases
CTDi | 191484 |
WormBasei | ZK994.3 ; CE40298 ; WBGene00004256 ; pxn-1 |
Phylogenomic databases
eggNOGi | KOG0619, Eukaryota KOG2408, Eukaryota |
GeneTreei | ENSGT00940000168557 |
HOGENOMi | CLU_006087_0_0_1 |
InParanoidi | Q1ENI8 |
OMAi | FHRKYRA |
OrthoDBi | 276568at2759 |
PhylomeDBi | Q1ENI8 |
Enzyme and pathway databases
Reactomei | R-CEL-209968, Thyroxine biosynthesis R-CEL-2243919, Crosslinking of collagen fibrils R-CEL-6798695, Neutrophil degranulation R-CEL-8941413, Events associated with phagocytolytic activity of PMN cells |
Miscellaneous databases
PROi | PR:Q1ENI8 |
Gene expression databases
Bgeei | WBGene00004256, Expressed in material anatomical entity and 4 other tissues |
Family and domain databases
CDDi | cd09826, peroxidasin_like, 1 hit |
Gene3Di | 1.10.640.10, 1 hit 2.60.40.10, 2 hits 3.80.10.10, 2 hits |
InterProi | View protein in InterPro IPR000483, Cys-rich_flank_reg_C IPR019791, Haem_peroxidase_animal IPR010255, Haem_peroxidase_sf IPR037120, Haem_peroxidase_sf_animal IPR007110, Ig-like_dom IPR036179, Ig-like_dom_sf IPR013783, Ig-like_fold IPR013098, Ig_I-set IPR003599, Ig_sub IPR003598, Ig_sub2 IPR001611, Leu-rich_rpt IPR003591, Leu-rich_rpt_typical-subtyp IPR032675, LRR_dom_sf IPR000372, LRRNT IPR034824, Peroxidasin_peroxidase IPR034826, Peroxidasin_PXN-1_nema |
PANTHERi | PTHR11475:SF74, PTHR11475:SF74, 1 hit |
Pfami | View protein in Pfam PF03098, An_peroxidase, 1 hit PF07679, I-set, 2 hits PF13855, LRR_8, 2 hits PF01462, LRRNT, 1 hit |
PRINTSi | PR00457, ANPEROXIDASE |
SMARTi | View protein in SMART SM00409, IG, 2 hits SM00408, IGc2, 2 hits SM00369, LRR_TYP, 3 hits SM00082, LRRCT, 1 hit SM00013, LRRNT, 1 hit |
SUPFAMi | SSF48113, SSF48113, 1 hit SSF48726, SSF48726, 2 hits |
PROSITEi | View protein in PROSITE PS50835, IG_LIKE, 2 hits PS50292, PEROXIDASE_3, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | PXDN1_CAEEL | |
Accessioni | Q1ENI8Primary (citable) accession number: Q1ENI8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 26, 2008 |
Last sequence update: | July 11, 2006 | |
Last modified: | May 25, 2022 | |
This is version 120 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Caenorhabditis annotation project |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Caenorhabditis elegans
Caenorhabditis elegans: entries, gene names and cross-references to WormBase - SIMILARITY comments
Index of protein domains and families