Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Folate synthesis bifunctional protein

Gene

CytHPPK/DHPS

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the first two consecutive steps of tetrahydrofolate biosynthesis (PubMed:17289662). Plays a role in seed stress response and survival (PubMed:17289662, PubMed:21996493).2 Publications

Catalytic activityi

ATP + 6-hydroxymethyl-7,8-dihydropterin = AMP + 6-hydroxymethyl-7,8-dihydropterin diphosphate.1 Publication
6-hydroxymethyl-7,8-dihydropterin diphosphate + 4-aminobenzoate = diphosphate + dihydropteroate.1 Publication

Cofactori

Mg2+By similarity

Enzyme regulationi

Inhibited by sulfanilamide.1 Publication

Kineticsi

  1. KM=30 µM for ATP1 Publication

    Pathwayi: tetrahydrofolate biosynthesis

    This protein is involved in step 4 of the subpathway that synthesizes 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate from 7,8-dihydroneopterin triphosphate.Curated
    Proteins known to be involved in the 4 steps of the subpathway in this organism are:
    1. no protein annotated in this organism
    2. no protein annotated in this organism
    3. Dihydroneopterin aldolase 2 (FOLB2), 7,8-dihydroneopterin aldolase (AXX17_At5g62530), 7,8-dihydroneopterin aldolase (AXX17_At3g23310), 7,8-dihydroneopterin aldolase (AXX17_At3g11690), Probable dihydroneopterin aldolase 3 (FOLB3), Dihydroneopterin aldolase 1 (FOLB1), 7,8-dihydroneopterin aldolase (AXX17_At3g11690)
    4. Folate synthesis bifunctional protein (CytHPPK/DHPS), Folate synthesis bifunctional protein, mitochondrial (MitHPPK/DHPS)
    This subpathway is part of the pathway tetrahydrofolate biosynthesis, which is itself part of Cofactor biosynthesis.
    View all proteins of this organism that are known to be involved in the subpathway that synthesizes 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate from 7,8-dihydroneopterin triphosphate, the pathway tetrahydrofolate biosynthesis and in Cofactor biosynthesis.

    Pathwayi: tetrahydrofolate biosynthesis

    This protein is involved in step 1 of the subpathway that synthesizes 7,8-dihydrofolate from 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate and 4-aminobenzoate.Curated
    Proteins known to be involved in the 2 steps of the subpathway in this organism are:
    1. Folate synthesis bifunctional protein (CytHPPK/DHPS), Folate synthesis bifunctional protein, mitochondrial (MitHPPK/DHPS)
    2. no protein annotated in this organism
    This subpathway is part of the pathway tetrahydrofolate biosynthesis, which is itself part of Cofactor biosynthesis.
    View all proteins of this organism that are known to be involved in the subpathway that synthesizes 7,8-dihydrofolate from 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate and 4-aminobenzoate, the pathway tetrahydrofolate biosynthesis and in Cofactor biosynthesis.

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Metal bindingi209MagnesiumBy similarity1
    Binding sitei2496-hydroxymethyl-7,8-dihydropterin diphosphateBy similarity1
    Binding sitei2866-hydroxymethyl-7,8-dihydropterin diphosphateBy similarity1
    Binding sitei3056-hydroxymethyl-7,8-dihydropterin diphosphateBy similarity1
    Binding sitei3786-hydroxymethyl-7,8-dihydropterin diphosphateBy similarity1
    Binding sitei4236-hydroxymethyl-7,8-dihydropterin diphosphateBy similarity1

    GO - Molecular functioni

    • 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine diphosphokinase activity Source: TAIR
    • ATP binding Source: UniProtKB-KW
    • dihydropteroate synthase activity Source: TAIR
    • kinase activity Source: UniProtKB-KW
    • metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    • folic acid biosynthetic process Source: UniProtKB-KW
    • response to oxidative stress Source: TAIR
    • tetrahydrofolate biosynthetic process Source: TAIR

    Keywordsi

    Molecular functionKinase, Multifunctional enzyme, Transferase
    Biological processFolate biosynthesis
    LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciARA:AT1G69190-MONOMER.
    BRENDAi2.5.1.15. 302.
    UniPathwayiUPA00077; UER00155.
    UPA00077; UER00156.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Folate synthesis bifunctional protein1 Publication
    Including the following 2 domains:
    6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase1 Publication (EC:2.7.6.31 Publication)
    Short name:
    HPPK1 Publication
    Alternative name(s):
    2-amino-4-hydroxy-6-hydroxymethyldihydropteridine diphosphokinase
    7,8-dihydro-6-hydroxymethylpterin-pyrophosphokinase
    Short name:
    PPPK
    Dihydropteroate synthase1 Publication (EC:2.5.1.151 Publication)
    Short name:
    DHPS1 Publication
    Gene namesi
    Name:CytHPPK/DHPS1 Publication
    Ordered Locus Names:At1g69190Imported
    ORF Names:F23O10.22Imported, F4N2.15Imported
    OrganismiArabidopsis thaliana (Mouse-ear cress)Imported
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    Proteomesi
    • UP000006548 Componenti: Chromosome 1

    Organism-specific databases

    AraportiAT1G69190.
    TAIRilocus:2026471. AT1G69190.

    Subcellular locationi

    Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Disruption phenotypei

    No visible phenotype (PubMed:17289662). Increased sensitivity to oxidative stress (PubMed:21996493).2 Publications

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00004328671 – 484Folate synthesis bifunctional proteinAdd BLAST484

    Proteomic databases

    PaxDbiQ1ENB6.

    PTM databases

    iPTMnetiQ1ENB6.

    Expressioni

    Tissue specificityi

    Expressed exclusively in reproductive tissues.1 Publication

    Developmental stagei

    Expressed in early stages of seed development, until the late globular stage of embryo. Not detected at the heart stage of embryo development.1 Publication

    Inductioni

    Up-regulated by slat stress (PubMed:17289662). Up-regulated by oxidative stress (PubMed:21996493).2 Publications

    Gene expression databases

    ExpressionAtlasiQ1ENB6. baseline and differential.
    GenevisibleiQ1ENB6. AT.

    Interactioni

    Protein-protein interaction databases

    STRINGi3702.AT1G69190.1.

    Structurei

    3D structure databases

    ProteinModelPortaliQ1ENB6.
    SMRiQ1ENB6.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Domaini202 – 470Pterin-bindingPROSITE-ProRule annotationAdd BLAST269

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Regioni15 – 141HPPKCuratedAdd BLAST127
    Regioni204 – 484DHPSAdd BLAST281
    Regioni458 – 4606-hydroxymethyl-7,8-dihydropterin diphosphate bindingBy similarity3

    Sequence similaritiesi

    In the N-terminal section; belongs to the HPPK family.Curated
    In the C-terminal section; belongs to the DHPS family.Curated

    Phylogenomic databases

    eggNOGiKOG2544. Eukaryota.
    COG0294. LUCA.
    COG0801. LUCA.
    HOGENOMiHOG000217511.
    KOiK13941.
    OMAiMELPKIR.
    OrthoDBiEOG093607KB.
    PhylomeDBiQ1ENB6.

    Family and domain databases

    CDDicd00739. DHPS. 1 hit.
    cd00483. HPPK. 1 hit.
    Gene3Di3.20.20.20. 1 hit.
    3.30.70.560. 1 hit.
    InterProiView protein in InterPro
    IPR006390. DHP_synth.
    IPR011005. Dihydropteroate_synth-like.
    IPR000550. Hppk.
    IPR035907. Hppk_sf.
    IPR000489. Pterin-binding_dom.
    PfamiView protein in Pfam
    PF01288. HPPK. 1 hit.
    PF00809. Pterin_bind. 1 hit.
    SUPFAMiSSF51717. SSF51717. 1 hit.
    SSF55083. SSF55083. 1 hit.
    TIGRFAMsiTIGR01496. DHPS. 1 hit.
    TIGR01498. folK. 1 hit.
    PROSITEiView protein in PROSITE
    PS00792. DHPS_1. 1 hit.
    PS00793. DHPS_2. 1 hit.
    PS00794. HPPK. 1 hit.
    PS50972. PTERIN_BINDING. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q1ENB6-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MDFTSLETTT FEEVVIALGS NVGNRMNNFK EALRLMKDYG ISVTRHSCLY
    60 70 80 90 100
    ETEPVHVTDQ PRFLNAAIRG VTKLKPHELL NVLKKIEKEM GREENGLRYG
    110 120 130 140 150
    PRPLDLDILF YGKHKIISDK LIIPHERIWE RPFVLAPLVD LLGTEDIDND
    160 170 180 190 200
    KIVAYWHSLS MHSGGIFQAW ERLGGESLLG KDGIIQRVIP IGDHLWDFSK
    210 220 230 240 250
    KTYVMGILNL TPDSFSDGGK FQSVDTAVSR VRSMISEGVD IIDIGAQSTR
    260 270 280 290 300
    PMASRISSQE EIDRLIPVLK VVRGMAEMKG KLISVDTFNS EVALEAIRNG
    310 320 330 340 350
    ADILNDVSGG SLDENMHKVV ADSDVPYMIM HMRGDPCTMQ NKENLEYNEI
    360 370 380 390 400
    CKDVATELYE RVREAELSGI PAWRIMIDPG IGFSKGIDHN LDIVMELPKI
    410 420 430 440 450
    REEMAKKSIG LSHAPILIGP SRKRFLGDIC GRPEASERDA ATVACVTAGI
    460 470 480
    LKGANIIRVH NVRDNVDAAR LCDAMMTKRF KNVD
    Length:484
    Mass (Da):54,110
    Last modified:July 11, 2006 - v1
    Checksum:i3F9985DBA8655369
    GO

    Sequence cautioni

    The sequence AAF27067 differs from that shown. Reason: Erroneous gene model prediction. Translation N-terminally extended.Curated
    The sequence AAG52504 differs from that shown. Reason: Erroneous gene model prediction. Translation N-terminally extended.Curated

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AJ866732 mRNA. Translation: CAI29255.1.
    AC008262 Genomic DNA. Translation: AAF27067.1. Sequence problems.
    AC018364 Genomic DNA. Translation: AAG52504.1. Sequence problems.
    CP002684 Genomic DNA. Translation: AEE34892.1.
    PIRiH96715.
    RefSeqiNP_001319354.1. NM_001334402.1.
    UniGeneiAt.70808.

    Genome annotation databases

    EnsemblPlantsiAT1G69190.1; AT1G69190.1; AT1G69190.
    GeneIDi843250.
    GrameneiAT1G69190.1; AT1G69190.1; AT1G69190.
    KEGGiath:AT1G69190.

    Similar proteinsi

    Entry informationi

    Entry nameiFOLC_ARATH
    AccessioniPrimary (citable) accession number: Q1ENB6
    Secondary accession number(s): Q9LE77
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 29, 2015
    Last sequence update: July 11, 2006
    Last modified: March 28, 2018
    This is version 97 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome