UniProtKB - Q19286 (IFB2_CAEEL)
Protein
Intermediate filament protein ifb-2
Gene
ifb-2
Organism
Caenorhabditis elegans
Status
Functioni
Cytoplasmic intermediate filaments provide mechanical strength to cells. Not essential protein. Component of the terminal web (organelle-depleted, intermediate filament-rich layer of cytoplasm that underlies the apical microvilli of polarized epithelial cells) in embryonic through to adult gut cells. Correct localization of filaments requires let-413.2 Publications
Names & Taxonomyi
Protein namesi | Recommended name: Intermediate filament protein ifb-2Alternative name(s): Cel IF B2 Intermediate filament protein B2 Short name: IF-B2 |
Gene namesi | Name:ifb-2 ORF Names:F10C1.7 |
Organismi | Caenorhabditis elegans |
Taxonomic identifieri | 6239 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Ecdysozoa › Nematoda › Chromadorea › Rhabditida › Rhabditina › Rhabditomorpha › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis |
Proteomesi |
|
Organism-specific databases
WormBasei | F10C1.7a ; CE02622 ; WBGene00002054 ; ifb-2 F10C1.7c ; CE27941 ; WBGene00002054 ; ifb-2 |
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm 1 Publication
Cytoskeleton
- intermediate filament Source: UniProtKB-KW
- terminal web Source: WormBase
Other locations
- apical cortex Source: WormBase
- cell junction Source: UniProtKB
- cytoplasm Source: UniProtKB
Keywords - Cellular componenti
Cytoplasm, Intermediate filamentPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000063839 | 1 – 543 | Intermediate filament protein ifb-2Add BLAST | 543 |
Proteomic databases
EPDi | Q19286 |
PaxDbi | Q19286 |
PeptideAtlasi | Q19286 |
PRIDEi | Q19286 |
2D gel databases
World-2DPAGEi | 0011:Q19286 0020:Q19286 |
PTM databases
iPTMneti | Q19286 |
Expressioni
Tissue specificityi
Expression is restricted to a discrete circumferential subapical layer within the intestinal terminal web (known as the 'endotube'); this layer joins directly to the apical junction complexes that connect adjacent gut cells.1 Publication
Developmental stagei
Lima bean stage of embryogenesis into adulthood.1 Publication
Gene expression databases
Bgeei | WBGene00002054, Expressed in multi-cellular organism and 3 other tissues |
ExpressionAtlasi | Q19286, baseline |
Interactioni
Binary interactionsi
Q19286
With | #Exp. | IntAct |
---|---|---|
CELE_Y56A3A.7 [A7DTF5] | 4 | EBI-320062,EBI-2413939 |
Protein-protein interaction databases
BioGRIDi | 39315, 16 interactors |
DIPi | DIP-26763N |
IntActi | Q19286, 12 interactors |
MINTi | Q19286 |
STRINGi | 6239.F10C1.7e |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 39 – 388 | IF rodPROSITE-ProRule annotationAdd BLAST | 350 | |
Domaini | 420 – 538 | LTDPROSITE-ProRule annotationAdd BLAST | 119 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 42 | HeadAdd BLAST | 42 | |
Regioni | 43 – 74 | Coil 1AAdd BLAST | 32 | |
Regioni | 75 – 88 | Linker 1Add BLAST | 14 | |
Regioni | 89 – 223 | Coil 1BAdd BLAST | 135 | |
Regioni | 224 – 240 | Linker 12Add BLAST | 17 | |
Regioni | 241 – 387 | Coil 2Add BLAST | 147 | |
Regioni | 388 – 542 | TailAdd BLAST | 155 |
Sequence similaritiesi
Belongs to the intermediate filament family.PROSITE-ProRule annotation
Keywords - Domaini
Coiled coilPhylogenomic databases
eggNOGi | KOG0977, Eukaryota |
GeneTreei | ENSGT00970000196708 |
InParanoidi | Q19286 |
PhylomeDBi | Q19286 |
Family and domain databases
Gene3Di | 1.20.5.1160, 1 hit 2.60.40.1260, 1 hit |
InterProi | View protein in InterPro IPR039008, IF_rod_dom IPR042180, IF_rod_dom_coil1B IPR016451, Intermed_filament_ifa/ifb IPR001322, Lamin_tail_dom IPR036415, Lamin_tail_dom_sf |
Pfami | View protein in Pfam PF00038, Filament, 1 hit |
PIRSFi | PIRSF005546, Intermed_filamnt_Ifb-2, 1 hit |
SMARTi | View protein in SMART SM01391, Filament, 1 hit |
SUPFAMi | SSF74853, SSF74853, 1 hit |
PROSITEi | View protein in PROSITE PS51842, IF_ROD_2, 1 hit PS51841, LTD, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All
Isoform a (identifier: Q19286-1) [UniParc]FASTAAdd to basket
Also known as: b2-H
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MSAVSYSMHR TTTTTSSSSH GGVSAGHAAE EFVASAEREK QEMQQLNSRL
60 70 80 90 100
EVYISRVRQL EDRNKELVIE LDTLRGSLGN DIGQIKFKFN DSLVKVRREI
110 120 130 140 150
SEAHSGTIGV EVKVDRLRDD LNDYRHRYEE ARREVEREKT TWGGAISQAQ
160 170 180 190 200
AELDTNKSRY AAILDEEKRL YAEQDQLYLQ LAAAKDELDA AIVDRRRLQA
210 220 230 240 250
EEDDLKIELE FLGRIHSQEI TELRTLLAQA PADTREFFKN ELALAIREIK
260 270 280 290 300
AEYDKIIQTT RVDLETIFQS KISAVESSIV SKNEAAVFRQ EEIRKMNESI
310 320 330 340 350
TTLRAKLSEL EARNSALERE ANTLQIQLGE DQRAYESELH KRDNALRFMR
360 370 380 390 400
EDCQTLIAEL QALLNTKQTL DTEIAIYRKL VESEEGRFTH VGQGVVVAQQ
410 420 430 440 450
ETTRLVPVEQ DHWDSGEVQT RSSFKRHAKG NVSIVECDPQ GKYIILENTS
460 470 480 490 500
GSVAEDVSNF EIRRVIDGVQ AFVFRLPSHL VIQQHGHLKI YGRNSGGINS
510 520 530 540
PPDSIVMESH PSWGQGGQVE TFLYNSHGIE KASHIQTTVA SSR
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketM1Z854 | M1Z854_CAEEL | Intermediate filament protein ifb-2 | ifb-2 CELE_F10C1.7, F10C1.7 | 577 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 532 | A → R in CAA55296 (PubMed:8013462).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_010148 | 388 – 404 | Missing in isoform c. CuratedAdd BLAST | 17 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X78553, X78554 Genomic DNA Translation: CAA55296.1 FO080600 Genomic DNA Translation: CCD65033.1 FO080600 Genomic DNA Translation: CCD65034.1 |
PIRi | T16015 |
RefSeqi | NP_495133.1, NM_062732.5 [Q19286-1] NP_495134.1, NM_062733.4 [Q19286-2] |
Genome annotation databases
EnsemblMetazoai | F10C1.7a.1; F10C1.7a.1; WBGene00002054 [Q19286-1] F10C1.7c.1; F10C1.7c.1; WBGene00002054 [Q19286-2] |
GeneIDi | 173973 |
UCSCi | F10C1.7c.1, c. elegans [Q19286-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X78553, X78554 Genomic DNA Translation: CAA55296.1 FO080600 Genomic DNA Translation: CCD65033.1 FO080600 Genomic DNA Translation: CCD65034.1 |
PIRi | T16015 |
RefSeqi | NP_495133.1, NM_062732.5 [Q19286-1] NP_495134.1, NM_062733.4 [Q19286-2] |
3D structure databases
SMRi | Q19286 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 39315, 16 interactors |
DIPi | DIP-26763N |
IntActi | Q19286, 12 interactors |
MINTi | Q19286 |
STRINGi | 6239.F10C1.7e |
PTM databases
iPTMneti | Q19286 |
2D gel databases
World-2DPAGEi | 0011:Q19286 0020:Q19286 |
Proteomic databases
EPDi | Q19286 |
PaxDbi | Q19286 |
PeptideAtlasi | Q19286 |
PRIDEi | Q19286 |
Genome annotation databases
EnsemblMetazoai | F10C1.7a.1; F10C1.7a.1; WBGene00002054 [Q19286-1] F10C1.7c.1; F10C1.7c.1; WBGene00002054 [Q19286-2] |
GeneIDi | 173973 |
UCSCi | F10C1.7c.1, c. elegans [Q19286-1] |
Organism-specific databases
CTDi | 173973 |
WormBasei | F10C1.7a ; CE02622 ; WBGene00002054 ; ifb-2 F10C1.7c ; CE27941 ; WBGene00002054 ; ifb-2 |
Phylogenomic databases
eggNOGi | KOG0977, Eukaryota |
GeneTreei | ENSGT00970000196708 |
InParanoidi | Q19286 |
PhylomeDBi | Q19286 |
Miscellaneous databases
PROi | PR:Q19286 |
Gene expression databases
Bgeei | WBGene00002054, Expressed in multi-cellular organism and 3 other tissues |
ExpressionAtlasi | Q19286, baseline |
Family and domain databases
Gene3Di | 1.20.5.1160, 1 hit 2.60.40.1260, 1 hit |
InterProi | View protein in InterPro IPR039008, IF_rod_dom IPR042180, IF_rod_dom_coil1B IPR016451, Intermed_filament_ifa/ifb IPR001322, Lamin_tail_dom IPR036415, Lamin_tail_dom_sf |
Pfami | View protein in Pfam PF00038, Filament, 1 hit |
PIRSFi | PIRSF005546, Intermed_filamnt_Ifb-2, 1 hit |
SMARTi | View protein in SMART SM01391, Filament, 1 hit |
SUPFAMi | SSF74853, SSF74853, 1 hit |
PROSITEi | View protein in PROSITE PS51842, IF_ROD_2, 1 hit PS51841, LTD, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | IFB2_CAEEL | |
Accessioni | Q19286Primary (citable) accession number: Q19286 Secondary accession number(s): Q21064, Q95QM2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 26, 2004 |
Last sequence update: | November 1, 1996 | |
Last modified: | April 7, 2021 | |
This is version 154 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Caenorhabditis annotation project |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Caenorhabditis elegans
Caenorhabditis elegans: entries, gene names and cross-references to WormBase - SIMILARITY comments
Index of protein domains and families