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Protein

Protein Dok-7

Gene

Dok7

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Probable muscle-intrinsic activator of MUSK that plays an essential role in neuromuscular synaptogenesis. Acts in aneural activation of MUSK and subsequent acetylcholine receptor (AchR) clustering in myotubes. Induces autophosphorylation of MUSK.2 Publications

GO - Molecular functioni

  • phosphatidylinositol binding Source: UniProtKB
  • protein kinase binding Source: UniProtKB

GO - Biological processi

Keywordsi

LigandLipid-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Protein Dok-7
Alternative name(s):
Downstream of tyrosine kinase 7
Gene namesi
Name:Dok7
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:3584043 Dok7

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Synapse

Pathology & Biotechi

Disruption phenotypei

Mice are immobile at birth and die shortly thereafter. They do not form neither acetylcholine receptor clusters nor neuromuscular synapses.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi158 – 159RR → AA: Abolishes interaction with MUSK and function; when associated with A-174. 1 Publication2
Mutagenesisi174R → A: Abolishes interaction with MUSK and function; when associated with A-158 and A-159. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002503721 – 504Protein Dok-7Add BLAST504

Proteomic databases

PaxDbiQ18PE0
PRIDEiQ18PE0

PTM databases

iPTMnetiQ18PE0
PhosphoSitePlusiQ18PE0

Expressioni

Developmental stagei

Expressed in the central region encompassing the endplate area of the diaphragm muscles at day 14.5 of embryonic development (E14.5), when AChRs cluster in a nerve- and agrin-independent manner.1 Publication

Gene expression databases

BgeeiENSMUSG00000044716 Expressed in 171 organ(s), highest expression level in quadriceps femoris
CleanExiMM_DOK7
ExpressionAtlasiQ18PE0 baseline and differential
GenevisibleiQ18PE0 MM

Interactioni

Subunit structurei

Homodimer. Forms a heterotetramer composed of 2 DOK7 and 2 MUSK molecules which facilitates MUSK trans-autophosphorylation on tyrosine residue and activation. Interacts (via IRS-type PTB domain) with MUSK (via cytoplasmic part); requires MUSK phosphorylation.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
MuskQ610063EBI-3989091,EBI-3989087

GO - Molecular functioni

Protein-protein interaction databases

ELMiQ18PE0
IntActiQ18PE0, 2 interactors
STRINGi10090.ENSMUSP00000059538

Structurei

Secondary structure

1504
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ18PE0
SMRiQ18PE0
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ18PE0

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini4 – 109PHPROSITE-ProRule annotationAdd BLAST106
Domaini105 – 210IRS-type PTBPROSITE-ProRule annotationAdd BLAST106

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi246 – 358Ser-richAdd BLAST113

Domaini

The PH domain mediated binding to phospholipids with phosphoinositol headgroups. Affinity is highest for phosphatidyl 3,4,5-trisphosphate, followed by phosphatidylinositol 3,4-bisphosphate and phosphatidylinositol 4,5-bisphosphate.1 Publication

Phylogenomic databases

eggNOGiENOG410IHI2 Eukaryota
ENOG4111MP1 LUCA
GeneTreeiENSGT00390000015386
HOGENOMiHOG000230953
HOVERGENiHBG080009
InParanoidiQ18PE0
OMAiEYQVPSS
PhylomeDBiQ18PE0
TreeFamiTF332288

Family and domain databases

CDDicd14677 PH_DOK7, 1 hit
cd13165 PTB_DOK7, 1 hit
Gene3Di2.30.29.30, 2 hits
InterProiView protein in InterPro
IPR037746 Dok-7
IPR037747 Dok-7_PH
IPR037748 Dok-7_PTB
IPR002404 IRS_PTB
IPR011993 PH-like_dom_sf
IPR001849 PH_domain
PANTHERiPTHR21636 PTHR21636, 1 hit
PfamiView protein in Pfam
PF02174 IRS, 1 hit
SMARTiView protein in SMART
SM00233 PH, 1 hit
PROSITEiView protein in PROSITE
PS51064 IRS_PTB, 1 hit
PS50003 PH_DOMAIN, 1 hit

Sequences (3+)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 3 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q18PE0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MTEAALVEGQ VKLRDGKKWK SRWLVLRKPS PVADCLLMLV YKDKCERSKG
60 70 80 90 100
LRERSSLTLE DICGLEPALP YEGLAHTLAI ICLSQAVMLG FDSHEAMCAW
110 120 130 140 150
DTRIRYALGE VHRFHVTVAP GTKLESGPAT LHLCNDILVL ARDIPPTVMG
160 170 180 190 200
QWKLSDLRRY GAVPNGFIFE GGTRCGYWAG VFFLSSAEGE QMSFLFDCIV
210 220 230 240 250
RGISPTKGPF GLRPVLPDPS SGGPSASEER VAQEALEALQ LEKRLSLLSH
260 270 280 290 300
SGRPGSGGDD RSLSSSSSEA SHSDISASSR LTAWPEQSSS SAGTSQEGPG
310 320 330 340 350
LVAAQGPGEA MLGASRPPLK PLRPRQLQEV GRQSSSDSGI ATGSHSSYSG
360 370 380 390 400
SFSSYAGSNL DVWRAGEEFG SLLSLPPGAS APEPRLCACP PGAAEYQVPT
410 420 430 440 450
SLRHHYDTPR SLRQAPRDPS PASQGSSDHG SATDLGGQAP TGCPSSWLGA
460 470 480 490 500
RRRGQATEGP GSDAALPSPS PGESWEAGSP HAGPPPAFFL SCSICGGLKV

KPPP
Length:504
Mass (Da):53,177
Last modified:July 25, 2006 - v1
Checksum:iAB0FC717F6C1A5B2
GO
Isoform 2 (identifier: Q18PE0-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     34-178: DCLLMLVYKD...FEGGTRCGYW → G

Note: No experimental confirmation available.
Show »
Length:360
Mass (Da):37,098
Checksum:iF8E9ABE394D5F37E
GO
Isoform 3 (identifier: Q18PE0-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     501-504: KPPP → MATSSPGFTV...PELEQRKKGP

Note: No experimental confirmation available.
Show »
Length:607
Mass (Da):64,260
Checksum:iA481EB490F5F9F96
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A0R4J0R2A0A0R4J0R2_MOUSE
Protein Dok-7
Dok7 A930013K19Rik, mCG_2539
570Annotation score:
D3Z7E3D3Z7E3_MOUSE
Protein Dok-7
Dok7
114Annotation score:

Sequence cautioni

The sequence AAH89590 differs from that shown. Reason: Erroneous initiation.Curated
The sequence BAC31923 differs from that shown. Reason: Erroneous initiation.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_02063634 – 178DCLLM…RCGYW → G in isoform 2. 1 PublicationAdd BLAST145
Alternative sequenceiVSP_020637501 – 504KPPP → MATSSPGFTVTHPGSPGRVA ADSPGPERPHSEMPTYVNIP ISPISRPQLHYMDLELPGAS AGVRGASTSRYAQIDIAATE TAHRVGVRHAQTREERLPEL EQRKKGP in isoform 3. 1 Publication4

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB220919 mRNA Translation: BAE96740.1
AK044445 mRNA Translation: BAC31923.1 Different initiation.
AK170454 mRNA Translation: BAE41809.1
BC089590 mRNA Translation: AAH89590.1 Different initiation.
RefSeqiNP_001335203.1, NM_001348274.1 [Q18PE0-1]
NP_001335204.1, NM_001348275.1
NP_001335205.1, NM_001348276.1 [Q18PE0-2]
NP_001335407.1, NM_001348478.1 [Q18PE0-3]
XP_006503961.1, XM_006503898.3 [Q18PE0-3]
XP_006503963.1, XM_006503900.2
UniGeneiMm.205483

Genome annotation databases

EnsembliENSMUST00000101298; ENSMUSP00000098856; ENSMUSG00000044716 [Q18PE0-2]
ENSMUST00000114270; ENSMUSP00000109909; ENSMUSG00000044716 [Q18PE0-1]
GeneIDi231134
KEGGimmu:231134
UCSCiuc008xdk.1 mouse [Q18PE0-1]
uc008xdl.1 mouse [Q18PE0-2]
uc008xdm.1 mouse [Q18PE0-3]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB220919 mRNA Translation: BAE96740.1
AK044445 mRNA Translation: BAC31923.1 Different initiation.
AK170454 mRNA Translation: BAE41809.1
BC089590 mRNA Translation: AAH89590.1 Different initiation.
RefSeqiNP_001335203.1, NM_001348274.1 [Q18PE0-1]
NP_001335204.1, NM_001348275.1
NP_001335205.1, NM_001348276.1 [Q18PE0-2]
NP_001335407.1, NM_001348478.1 [Q18PE0-3]
XP_006503961.1, XM_006503898.3 [Q18PE0-3]
XP_006503963.1, XM_006503900.2
UniGeneiMm.205483

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3ML4X-ray2.60A/B/C/D1-220[»]
ProteinModelPortaliQ18PE0
SMRiQ18PE0
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

ELMiQ18PE0
IntActiQ18PE0, 2 interactors
STRINGi10090.ENSMUSP00000059538

PTM databases

iPTMnetiQ18PE0
PhosphoSitePlusiQ18PE0

Proteomic databases

PaxDbiQ18PE0
PRIDEiQ18PE0

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000101298; ENSMUSP00000098856; ENSMUSG00000044716 [Q18PE0-2]
ENSMUST00000114270; ENSMUSP00000109909; ENSMUSG00000044716 [Q18PE0-1]
GeneIDi231134
KEGGimmu:231134
UCSCiuc008xdk.1 mouse [Q18PE0-1]
uc008xdl.1 mouse [Q18PE0-2]
uc008xdm.1 mouse [Q18PE0-3]

Organism-specific databases

CTDi285489
MGIiMGI:3584043 Dok7

Phylogenomic databases

eggNOGiENOG410IHI2 Eukaryota
ENOG4111MP1 LUCA
GeneTreeiENSGT00390000015386
HOGENOMiHOG000230953
HOVERGENiHBG080009
InParanoidiQ18PE0
OMAiEYQVPSS
PhylomeDBiQ18PE0
TreeFamiTF332288

Miscellaneous databases

EvolutionaryTraceiQ18PE0
PROiPR:Q18PE0
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000044716 Expressed in 171 organ(s), highest expression level in quadriceps femoris
CleanExiMM_DOK7
ExpressionAtlasiQ18PE0 baseline and differential
GenevisibleiQ18PE0 MM

Family and domain databases

CDDicd14677 PH_DOK7, 1 hit
cd13165 PTB_DOK7, 1 hit
Gene3Di2.30.29.30, 2 hits
InterProiView protein in InterPro
IPR037746 Dok-7
IPR037747 Dok-7_PH
IPR037748 Dok-7_PTB
IPR002404 IRS_PTB
IPR011993 PH-like_dom_sf
IPR001849 PH_domain
PANTHERiPTHR21636 PTHR21636, 1 hit
PfamiView protein in Pfam
PF02174 IRS, 1 hit
SMARTiView protein in SMART
SM00233 PH, 1 hit
PROSITEiView protein in PROSITE
PS51064 IRS_PTB, 1 hit
PS50003 PH_DOMAIN, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiDOK7_MOUSE
AccessioniPrimary (citable) accession number: Q18PE0
Secondary accession number(s): Q3TCZ6, Q5FW70, Q8C8U7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 19, 2006
Last sequence update: July 25, 2006
Last modified: November 7, 2018
This is version 99 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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