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Protein

Acetate kinase

Gene

ackA

Organism
Peptoclostridium difficile (strain 630) (Clostridium difficile)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the formation of acetyl phosphate from acetate and ATP. Can also catalyze the reverse reaction.UniRule annotation

Catalytic activityi

ATP + acetate = ADP + acetyl phosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation, Mn2+UniRule annotationNote: Mg2+. Can also accept Mn2+.UniRule annotation

Pathwayi: acetyl-CoA biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes acetyl-CoA from acetate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Acetate kinase (ackA)
  2. no protein annotated in this organism
This subpathway is part of the pathway acetyl-CoA biosynthesis, which is itself part of Metabolic intermediate biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes acetyl-CoA from acetate, the pathway acetyl-CoA biosynthesis and in Metabolic intermediate biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi7MagnesiumUniRule annotation1
Binding sitei14ATPUniRule annotation1
Binding sitei92SubstrateUniRule annotation1
Active sitei149Proton donor/acceptorUniRule annotation1
Sitei181Transition state stabilizerUniRule annotation1
Sitei242Transition state stabilizerUniRule annotation1
Metal bindingi385MagnesiumUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi209 – 213ATPUniRule annotation5
Nucleotide bindingi284 – 286ATPUniRule annotation3
Nucleotide bindingi332 – 336ATPUniRule annotation5

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciPDIF272563:G12WB-1305-MONOMER
UniPathwayi
UPA00340;UER00458

Names & Taxonomyi

Protein namesi
Recommended name:
Acetate kinaseUniRule annotation (EC:2.7.2.1UniRule annotation)
Alternative name(s):
AcetokinaseUniRule annotation
Gene namesi
Name:ackAUniRule annotation
Ordered Locus Names:CD630_11750
OrganismiPeptoclostridium difficile (strain 630) (Clostridium difficile)
Taxonomic identifieri272563 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesPeptostreptococcaceaeClostridioides
Proteomesi
  • UP000001978 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000022231 – 398Acetate kinaseAdd BLAST398

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi272563.CD1175

Structurei

3D structure databases

ProteinModelPortaliQ18B36
SMRiQ18B36
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the acetokinase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C6H Bacteria
COG0282 LUCA
HOGENOMiHOG000288398
KOiK00925
OMAiWKALTGT

Family and domain databases

HAMAPiMF_00020 Acetate_kinase, 1 hit
InterProiView protein in InterPro
IPR004372 Ac/propionate_kinase
IPR000890 Aliphatic_acid_kin_short-chain
IPR023865 Aliphatic_acid_kinase_CS
PANTHERiPTHR21060 PTHR21060, 1 hit
PfamiView protein in Pfam
PF00871 Acetate_kinase, 1 hit
PIRSFiPIRSF000722 Acetate_prop_kin, 1 hit
PRINTSiPR00471 ACETATEKNASE
TIGRFAMsiTIGR00016 ackA, 1 hit
PROSITEiView protein in PROSITE
PS01075 ACETATE_KINASE_1, 1 hit
PS01076 ACETATE_KINASE_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q18B36-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MKILVLNCGS SSLKYQLIDM NNEEVLCIGL VERIGIEGSI LKHEKAGRDD
60 70 80 90 100
KYVVEQPMKD HKDAIALVLE AVAHPEFGAV KEMKEIDAVG HRVVHAGEKF
110 120 130 140 150
ATSVVITPEV EEALKECIDL APLHNPANIM GIDACKAILP DVPMVGVFDT
160 170 180 190 200
AFHQTMPKSS YLYGLPHELY TKYGVRRYGF HGTSHNYVSQ RAAEILGKDI
210 220 230 240 250
KDLKIVTCHL GNGASIAAVD GGKCVDTSMG FTPLEGLIMG TRCGDIDPAI
260 270 280 290 300
LPFLMRKEGL DADGLDKLMN KESGVYGMTG ISSDFRDIED AAKNGDERAQ
310 320 330 340 350
ATLEAYVKKV QKYIGAYAAE MNGLDVVVFT AGVGENGKAI RADIASNMEF
360 370 380 390
LGMKLDKEAN DVRGKETVIS TADSKVKMLL IPTNEELMIA RDTLRLVK
Length:398
Mass (Da):43,320
Last modified:July 25, 2006 - v1
Checksum:i2C94037BFFF14BC4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM180355 Genomic DNA Translation: CAJ68028.1
RefSeqiWP_003438096.1, NZ_CP010905.2
YP_001087667.1, NC_009089.1

Genome annotation databases

EnsemblBacteriaiCAJ68028; CAJ68028; CD630_11750
GeneIDi31352752
4916377
KEGGicdf:CD630_11750
pdc:CDIF630_01323
PATRICifig|272563.120.peg.1225

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM180355 Genomic DNA Translation: CAJ68028.1
RefSeqiWP_003438096.1, NZ_CP010905.2
YP_001087667.1, NC_009089.1

3D structure databases

ProteinModelPortaliQ18B36
SMRiQ18B36
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272563.CD1175

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAJ68028; CAJ68028; CD630_11750
GeneIDi31352752
4916377
KEGGicdf:CD630_11750
pdc:CDIF630_01323
PATRICifig|272563.120.peg.1225

Phylogenomic databases

eggNOGiENOG4105C6H Bacteria
COG0282 LUCA
HOGENOMiHOG000288398
KOiK00925
OMAiWKALTGT

Enzyme and pathway databases

UniPathwayi
UPA00340;UER00458

BioCyciPDIF272563:G12WB-1305-MONOMER

Family and domain databases

HAMAPiMF_00020 Acetate_kinase, 1 hit
InterProiView protein in InterPro
IPR004372 Ac/propionate_kinase
IPR000890 Aliphatic_acid_kin_short-chain
IPR023865 Aliphatic_acid_kinase_CS
PANTHERiPTHR21060 PTHR21060, 1 hit
PfamiView protein in Pfam
PF00871 Acetate_kinase, 1 hit
PIRSFiPIRSF000722 Acetate_prop_kin, 1 hit
PRINTSiPR00471 ACETATEKNASE
TIGRFAMsiTIGR00016 ackA, 1 hit
PROSITEiView protein in PROSITE
PS01075 ACETATE_KINASE_1, 1 hit
PS01076 ACETATE_KINASE_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiACKA_PEPD6
AccessioniPrimary (citable) accession number: Q18B36
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: July 25, 2006
Last modified: October 10, 2018
This is version 75 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
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Main funding by: National Institutes of Health

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