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UniProtKB - Q18788 (MAN12_CAEEL)
Protein
Mannosyl-oligosaccharide 1,2-alpha-mannosidase C52E4.5
Gene
C52E4.5
Organism
Caenorhabditis elegans
Status
Functioni
Involved in the maturation of Asn-linked oligosaccharides. Progressively trim alpha-1,2-linked mannose residues from Man9GlcNAc2 to produce Man5GlcNAc2 (By similarity).
By similarityCatalytic activityi
- 4 H2O + N4-(α-D-Man-(1→2)-α-D-Man-(1→2)-α-D-Man-(1→3)-[α-D-Man-(1→2)-α-D-Man-(1→3)-[α-D-Man-(1→2)-α-D-Man-(1→6)]-α-D-Man-(1→6)]-β-D-Man-(1→4)-β-D-GlcNAc-(1→4)-α-D-GlcNAc)-L-asparaginyl-[protein] (N-glucan mannose isomer 9A1,2,3B1,2,3) = 4 β-D-mannose + N4-(α-D-Man-(1→3)-[α-D-Man-(1→3)-[α-D-Man-(1→6)]-α-D-Man-(1→6)]-β-D-Man-(1→4)-β-D-GlcNAc-(1→4)-β-D-GlcNAc)-L-asparaginyl-[protein] (N-glucan mannose isomer 5A1,2)By similarityEC:3.2.1.113By similarity
- 3 H2O + N4-(α-D-Man-(1→2)-α-D-Man-(1→2)-α-D-Man-(1→3)-[α-D-Man-(1→3)-[α-D-Man-(1→2)-α-D-Man-(1→6)]-α-D-Man-(1→6)]-β-D-Man-(1→4)-β-D-GlcNAc-(1→4)-α-D-GlcNAc)-L-asparaginyl-[protein] (N-glucan mannose isomer 8A1,2,3B1,3) = 3 β-D-mannose + N4-(α-D-Man-(1→3)-[α-D-Man-(1→3)-[α-D-Man-(1→6)]-α-D-Man-(1→6)]-β-D-Man-(1→4)-β-D-GlcNAc-(1→4)-β-D-GlcNAc)-L-asparaginyl-[protein] (N-glucan mannose isomer 5A1,2)By similarityEC:3.2.1.113By similarity
Cofactori
Ca2+By similarity
: protein glycosylation Pathwayi
This protein is involved in the pathway protein glycosylation, which is part of Protein modification.By similarityView all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 470 | Proton donorBy similarity | 1 | |
Metal bindingi | 580 | CalciumBy similarity | 1 |
GO - Molecular functioni
- calcium ion binding Source: InterPro
- mannosyl-oligosaccharide 1,2-alpha-mannosidase activity Source: GO_Central
GO - Biological processi
- carbohydrate metabolic process Source: InterPro
- N-glycan processing Source: GO_Central
- protein glycosylation Source: UniProtKB-UniPathway
Keywordsi
Molecular function | Glycosidase, Hydrolase |
Ligand | Calcium, Metal-binding |
Enzyme and pathway databases
Reactomei | R-CEL-964827, Progressive trimming of alpha-1,2-linked mannose residues from Man9/8/7GlcNAc2 to produce Man5GlcNAc2 |
UniPathwayi | UPA00378 |
Protein family/group databases
CAZyi | GH47, Glycoside Hydrolase Family 47 |
Names & Taxonomyi
Protein namesi | Recommended name: Mannosyl-oligosaccharide 1,2-alpha-mannosidase C52E4.5 (EC:3.2.1.113By similarity)Alternative name(s): Processing alpha-1,2-mannosidase C52E4.5 Short name: Alpha-1,2-mannosidase C52E4.5 |
Gene namesi | ORF Names:C52E4.5 |
Organismi | Caenorhabditis elegans |
Taxonomic identifieri | 6239 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Ecdysozoa › Nematoda › Chromadorea › Rhabditida › Rhabditina › Rhabditomorpha › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis |
Proteomesi |
|
Organism-specific databases
WormBasei | C52E4.5a ; CE08947 ; WBGene00008258 C52E4.5b ; CE45297 ; WBGene00008258 |
Subcellular locationi
Other locations
Endoplasmic reticulum
- endoplasmic reticulum Source: GO_Central
Golgi apparatus
- Golgi membrane Source: GO_Central
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 9 | CytoplasmicSequence analysis | 9 | |
Transmembranei | 10 – 30 | Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST | 21 | |
Topological domaini | 31 – 590 | LumenalSequence analysisAdd BLAST | 560 |
Keywords - Cellular componenti
MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000248514 | 1 – 590 | Mannosyl-oligosaccharide 1,2-alpha-mannosidase C52E4.5Add BLAST | 590 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 156 | N-linked (GlcNAc...) asparagine1 Publication | 1 | |
Glycosylationi | 212 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 373 | N-linked (GlcNAc...) asparagine2 Publications | 1 | |
Glycosylationi | 402 | N-linked (GlcNAc...) asparagine1 Publication | 1 | |
Disulfide bondi | 423 ↔ 456 | By similarity |
Keywords - PTMi
Disulfide bond, GlycoproteinProteomic databases
EPDi | Q18788 |
PaxDbi | Q18788 |
PeptideAtlasi | Q18788 |
PRIDEi | Q18788 |
PTM databases
iPTMneti | Q18788 |
Expressioni
Gene expression databases
Bgeei | WBGene00008258, Expressed in material anatomical entity and 4 other tissues |
Interactioni
Protein-protein interaction databases
BioGRIDi | 44666, 1 interactor |
IntActi | Q18788, 1 interactor |
MINTi | Q18788 |
STRINGi | 6239.C52E4.5b |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 88 – 112 | DisorderedSequence analysisAdd BLAST | 25 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 88 – 105 | Basic and acidic residuesSequence analysisAdd BLAST | 18 |
Sequence similaritiesi
Belongs to the glycosyl hydrolase 47 family.Curated
Keywords - Domaini
Signal-anchor, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG2204, Eukaryota |
GeneTreei | ENSGT00940000167910 |
HOGENOMi | CLU_003818_3_2_1 |
InParanoidi | Q18788 |
OMAi | EVVPCED |
OrthoDBi | 693882at2759 |
PhylomeDBi | Q18788 |
Family and domain databases
Gene3Di | 1.50.10.10, 1 hit |
InterProi | View protein in InterPro IPR012341, 6hp_glycosidase-like_sf IPR001382, Glyco_hydro_47 IPR036026, Seven-hairpin_glycosidases |
Pfami | View protein in Pfam PF01532, Glyco_hydro_47, 1 hit |
PRINTSi | PR00747, GLYHDRLASE47 |
SUPFAMi | SSF48225, SSF48225, 1 hit |
s (2)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketIsoform a (identifier: Q18788-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MKTVRFNKQA LAILAACFIF LLCVVCYFSA SSESHNAVVV GERARGHIAV
60 70 80 90 100
RDVENRHLAE EKHAVIHAKT VGKIERVVSQ EKVEILRPAR VESKPPGEKT
110 120 130 140 150
STEPEETGVG KAPIQTSEGL EKLIGKIHYE NKDEENDLRR QKVKEMMIHA
160 170 180 190 200
WEGYKNYSWG ANELRPMSKK PNSQNIFGGS QMPATIVDAA DTLFIMDLKD
210 220 230 240 250
KYKEARDYIE NNFSMAKSTS TLSVFETTIR FLGGLLSLYA LTQESFYIEK
260 270 280 290 300
AREVGEALLP AFNTPSGIPK SNLDVASKHA SNYGWANGGQ SILSEIGSLH
310 320 330 340 350
LEFLYLSRIS NAPIFEKKVK KVRDALEKAE KPNGLYSNYI NPDTGKFTGS
360 370 380 390 400
HMSLGALGDS FYEYLIKSYV QSNYTDTQAK NMYWDVSDAI QKHMIKVSKQ
410 420 430 440 450
SNLTYTVELN NGQAQHKMGH LACFVPGMFA LQAINEDTEE EKLRIMTLAE
460 470 480 490 500
ELAKTCHESY IRSETHIGPE MFYFNERDEA TSKHSENGYI QRPEVIEGWF
510 520 530 540 550
YLWRLTGKTM YRDWVWDAVQ AIEKYCRVDS GFTGLQNVYN PKAGREDVMQ
560 570 580 590
SFFLAEFLKY AYLTFADESL ISLDKWVFNT EAHPVPVLTN
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_044102 | 1 | M → MIDVSSSITNMNVTIVESFL DFFKILAYVSKFSSWKEPNK TTFPHAM in isoform b. Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z78012 Genomic DNA Translation: CAB01415.1 Z78012 Genomic DNA Translation: CBW48349.1 |
PIRi | T20153 |
RefSeqi | NP_001256388.1, NM_001269459.1 NP_001256389.1, NM_001269460.1 [Q18788-1] |
Genome annotation databases
EnsemblMetazoai | C52E4.5.1; C52E4.5.1; WBGene00008258 [Q18788-1] C52E4.5.2; C52E4.5.2; WBGene00008258 [Q18788-1] |
GeneIDi | 179642 |
KEGGi | cel:CELE_C52E4.5 |
UCSCi | C52E4.5, c. elegans [Q18788-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z78012 Genomic DNA Translation: CAB01415.1 Z78012 Genomic DNA Translation: CBW48349.1 |
PIRi | T20153 |
RefSeqi | NP_001256388.1, NM_001269459.1 NP_001256389.1, NM_001269460.1 [Q18788-1] |
3D structure databases
SMRi | Q18788 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 44666, 1 interactor |
IntActi | Q18788, 1 interactor |
MINTi | Q18788 |
STRINGi | 6239.C52E4.5b |
Protein family/group databases
CAZyi | GH47, Glycoside Hydrolase Family 47 |
PTM databases
iPTMneti | Q18788 |
Proteomic databases
EPDi | Q18788 |
PaxDbi | Q18788 |
PeptideAtlasi | Q18788 |
PRIDEi | Q18788 |
Genome annotation databases
EnsemblMetazoai | C52E4.5.1; C52E4.5.1; WBGene00008258 [Q18788-1] C52E4.5.2; C52E4.5.2; WBGene00008258 [Q18788-1] |
GeneIDi | 179642 |
KEGGi | cel:CELE_C52E4.5 |
UCSCi | C52E4.5, c. elegans [Q18788-1] |
Organism-specific databases
CTDi | 179642 |
WormBasei | C52E4.5a ; CE08947 ; WBGene00008258 C52E4.5b ; CE45297 ; WBGene00008258 |
Phylogenomic databases
eggNOGi | KOG2204, Eukaryota |
GeneTreei | ENSGT00940000167910 |
HOGENOMi | CLU_003818_3_2_1 |
InParanoidi | Q18788 |
OMAi | EVVPCED |
OrthoDBi | 693882at2759 |
PhylomeDBi | Q18788 |
Enzyme and pathway databases
UniPathwayi | UPA00378 |
Reactomei | R-CEL-964827, Progressive trimming of alpha-1,2-linked mannose residues from Man9/8/7GlcNAc2 to produce Man5GlcNAc2 |
Miscellaneous databases
PROi | PR:Q18788 |
Gene expression databases
Bgeei | WBGene00008258, Expressed in material anatomical entity and 4 other tissues |
Family and domain databases
Gene3Di | 1.50.10.10, 1 hit |
InterProi | View protein in InterPro IPR012341, 6hp_glycosidase-like_sf IPR001382, Glyco_hydro_47 IPR036026, Seven-hairpin_glycosidases |
Pfami | View protein in Pfam PF01532, Glyco_hydro_47, 1 hit |
PRINTSi | PR00747, GLYHDRLASE47 |
SUPFAMi | SSF48225, SSF48225, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | MAN12_CAEEL | |
Accessioni | Q18788Primary (citable) accession number: Q18788 Secondary accession number(s): E1B6T6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | September 5, 2006 |
Last sequence update: | November 1, 1996 | |
Last modified: | February 23, 2022 | |
This is version 148 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Caenorhabditis annotation project |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Glycosyl hydrolases
Classification of glycosyl hydrolase families and list of entries - Caenorhabditis elegans
Caenorhabditis elegans: entries, gene names and cross-references to WormBase - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families