UniProtKB - Q18014 (OFUT1_CAEEL)

BLAST

>sp|Q18014|OFUT1_CAEEL GDP-fucose protein O-fucosyltransferase 1 OS=Caenorhabditis elegans OX=6239 GN=C15C7.7 PE=1 SV=5
MRVSKVLTLASFISVCSYSEAKSNETDPNGYIVFCPCMGRFGNQVDQFLGVLAFAKALDR
TLVLPNFIEFKHPETKMIPFEFLFQVGTVAKYTRVVTMQEFTKKIMPTVWPPEKRKAFCW
TPRQAIYDKSAEPGCHSKEGNPFGPYWDQIDVSFVGDEYFGDIPGGFDLNQMGSRKKWLE
KFPSEEYPVLAFSSAPAPFPSKGKVWSIQKYLRWSSRITEQAKKFISANLAKPFVAVHLR
NDADWVRVCEHIDTTTNRPLFASEQCLGEGHHLGTLTKEICSPSKQQILEQIVEKVGSIG
AKSVFVASDKDHMIDEINEALKPYEIEAHRQEPDDMYTSLAIMGRADLFVGNCVSTFSHI
VKRERDHAGQSPRPSAFFGIRAVKRHIDL

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History
Entry version 113 (07 Nov 2018)
Sequence version 5 (01 Feb 2005)
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Protein

GDP-fucose protein O-fucosyltransferase 1

Gene

C15C7.7

Organism

Caenorhabditis elegans

Status

Reviewed-Annotation score: -Experimental evidence at protein levelⁱ

Functionⁱ

Catalyzes the reaction that attaches fucose through an O-glycosidic linkage to a conserved serine or threonine residue in EGF domains.1 Publication

Miscellaneous

Manganese is bound to the substrate GDP-fucose, but is not required for enzyme activity.1 Publication

Catalytic activityⁱ

Transfers an alpha-L-fucosyl residue from GDP-beta-L-fucose to the serine hydroxy group of a protein acceptor.1 Publication

Pathwayⁱ: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

GO - Molecular functionⁱ

fucosyltransferase activity
Source: UniProtKB
Inferred from sequence or structural similarityⁱ
- 11524432
peptide-O-fucosyltransferase activity
Source: WormBase
Inferred from direct assayⁱ
- 21966509

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

fucose metabolic process Source: UniProtKB-KW
Notch signaling pathway Source: UniProtKB-KW
protein O-linked fucosylation
Source: WormBase
Inferred from direct assayⁱ
- 21966509
protein O-linked glycosylation
Source: UniProtKB
Inferred from sequence or structural similarityⁱ
- 11524432

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Glycosyltransferase, Transferase
Biological process	Carbohydrate metabolism, Fucose metabolism, Notch signaling pathway
Ligand	Manganese

Enzyme and pathway databases

BRENDAⁱ	2.4.1.221 1045
UniPathwayⁱ	UPA00378

Protein family/group databases

Carbohydrate-Active enZymes More...CAZyⁱ	GT65 Glycosyltransferase Family 65

CAZyⁱ

GT65 Glycosyltransferase Family 65

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: GDP-fucose protein O-fucosyltransferase 1 (EC:2.4.1.221) Alternative name(s): Peptide-O-fucosyltransferase 1 Short name: O-FucT-1
Gene namesⁱ	ORF Names:C15C7.7
Organismⁱ	Caenorhabditis elegans
Taxonomic identifierⁱ	6239 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Nematoda › Chromadorea › Rhabditida › Rhabditina › Rhabditomorpha › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis
Proteomesⁱ	UP000001940 Componentⁱ: Chromosome X

Organism-specific databases

WormBase More...WormBaseⁱ	C15C7.7 ; CE36786 ; WBGene00015793

WormBaseⁱ

C15C7.7 ; CE36786 ; WBGene00015793

Keywords - Cellular componentⁱ

Endoplasmic reticulum

Pathology & Biotechⁱ

Mutagenesis

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Mutagenesisⁱ	43	N → A: Reduces enzyme activity by over 90%. 1 Publication		1
Mutagenesisⁱ	240	R → A or K: Abolishes enzyme activity. 1 Publication		1

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Signal peptideⁱ	1 – 21	Sequence analysisAdd BLAST		21
ChainⁱPRO_0000012152	22 – 389	GDP-fucose protein O-fucosyltransferase 1Add BLAST		368

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Length
Glycosylationⁱ	24	N-linked (GlcNAc...) asparagineCurated	1
Disulfide bondⁱ	35 ↔ 37	1 Publication
Disulfide bondⁱ	119 ↔ 135	1 Publication
Disulfide bondⁱ	249 ↔ 281	1 Publication
Disulfide bondⁱ	266 ↔ 353	1 Publication

Keywords - PTMⁱ

Disulfide bond, Glycoprotein

Proteomic databases

EPDⁱ	Q18014
PaxDbⁱ	Q18014
PeptideAtlas More...PeptideAtlasⁱ	Q18014
PRIDEⁱ	Q18014

Expressionⁱ

Gene expression databases

Bgeeⁱ	WBGene00015793 Expressed in 5 organ(s), highest expression level in material anatomical entity

Bgeeⁱ

WBGene00015793 Expressed in 5 organ(s), highest expression level in material anatomical entity

Interactionⁱ

Subunit structureⁱ

Monomer.1 Publication

Protein-protein interaction databases

STRINGⁱ	6239.C15C7.7

STRINGⁱ

6239.C15C7.7

Structureⁱ

Secondary structure

Legend: HelixTurnBeta strandPDB Structure known for this area

Feature key	Position(s)	DescriptionActions	Length
Beta strandⁱ	31 – 34	Combined sources	4
Beta strandⁱ	38 – 40	Combined sources	3
Helixⁱ	41 – 58	Combined sources	18
Beta strandⁱ	61 – 63	Combined sources	3
Beta strandⁱ	67 – 69	Combined sources	3
Beta strandⁱ	71 – 74	Combined sources	4
Beta strandⁱ	76 – 78	Combined sources	3
Helixⁱ	80 – 83	Combined sources	4
Helixⁱ	86 – 89	Combined sources	4
Turnⁱ	90 – 92	Combined sources	3
Helixⁱ	98 – 104	Combined sources	7
Helixⁱ	106 – 109	Combined sources	4
Helixⁱ	112 – 114	Combined sources	3
Beta strandⁱ	116 – 121	Combined sources	6
Helixⁱ	143 – 149	Combined sources	7
Beta strandⁱ	156 – 159	Combined sources	4
Turnⁱ	164 – 167	Combined sources	4
Beta strandⁱ	170 – 172	Combined sources	3
Helixⁱ	178 – 181	Combined sources	4
Turnⁱ	184 – 186	Combined sources	3
Beta strandⁱ	188 – 194	Combined sources	7
Helixⁱ	203 – 211	Combined sources	9
Helixⁱ	216 – 229	Combined sources	14
Beta strandⁱ	232 – 239	Combined sources	8
Helixⁱ	243 – 250	Combined sources	8
Turnⁱ	254 – 256	Combined sources	3
Turnⁱ	261 – 263	Combined sources	3
Helixⁱ	264 – 267	Combined sources	4
Helixⁱ	269 – 271	Combined sources	3
Helixⁱ	278 – 281	Combined sources	4
Helixⁱ	285 – 299	Combined sources	15
Beta strandⁱ	302 – 310	Combined sources	9
Helixⁱ	314 – 321	Combined sources	8
Helixⁱ	322 – 324	Combined sources	3
Helixⁱ	336 – 345	Combined sources	10
Beta strandⁱ	346 – 351	Combined sources	6
Helixⁱ	356 – 367	Combined sources	12
Beta strandⁱ	368 – 371	Combined sources	4
Beta strandⁱ	375 – 377	Combined sources	3

Show more details

3D structure databases

ProteinModelPortalⁱ	Q18014
SMRⁱ	Q18014
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Miscellaneous databases

EvolutionaryTraceⁱ

Q18014

Family & Domainsⁱ

Region

Feature key	Position(s)	DescriptionActions	Length
Regionⁱ	40 – 43	Substrate bindingCombined sources	4
Regionⁱ	238 – 240	Substrate bindingCombined sources	3
Regionⁱ	356 – 357	Substrate bindingCombined sources1 Publication	2

Sequence similaritiesⁱ

Belongs to the glycosyltransferase 65 family.Curated

Keywords - Domainⁱ

Signal

Phylogenomic databases

eggNOGⁱ	KOG3202 Eukaryota KOG3849 Eukaryota ENOG410Y3JQ LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00390000015634
HOGENOMⁱ	HOG000231651
InParanoidⁱ	Q18014
KEGG Orthology (KO) More...KOⁱ	K03691
OMAⁱ	GQSNHFI
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G048B
PhylomeDBⁱ	Q18014

Family and domain databases

InterProⁱ	View protein in InterPro IPR019378 GDP-Fuc_O-FucTrfase IPR039922 POFUT1
PANTHERⁱ	PTHR21420 PTHR21420, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF10250 O-FucT, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

Sequence processingⁱ: The displayed sequence is further processed into a mature form.

Q18014-1 [UniParc]FASTA Add to basket

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        10         20         30         40         50
MRVSKVLTLA SFISVCSYSE AKSNETDPNG YIVFCPCMGR FGNQVDQFLG 
        60         70         80         90        100
VLAFAKALDR TLVLPNFIEF KHPETKMIPF EFLFQVGTVA KYTRVVTMQE 
       110        120        130        140        150
FTKKIMPTVW PPEKRKAFCW TPRQAIYDKS AEPGCHSKEG NPFGPYWDQI 
       160        170        180        190        200
DVSFVGDEYF GDIPGGFDLN QMGSRKKWLE KFPSEEYPVL AFSSAPAPFP 
       210        220        230        240        250
SKGKVWSIQK YLRWSSRITE QAKKFISANL AKPFVAVHLR NDADWVRVCE 
       260        270        280        290        300
HIDTTTNRPL FASEQCLGEG HHLGTLTKEI CSPSKQQILE QIVEKVGSIG 
       310        320        330        340        350
AKSVFVASDK DHMIDEINEA LKPYEIEAHR QEPDDMYTSL AIMGRADLFV 
       360        370        380 
GNCVSTFSHI VKRERDHAGQ SPRPSAFFGI RAVKRHIDL

Length:389

Mass (Da):44,051

Last modified:February 1, 2005 - v5

Checksum:ⁱ0C84F202AA184554

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	FO080550 Genomic DNA Translation: CCD64591.1
NCBI Reference Sequences More...RefSeqⁱ	NP_741744.2, NM_171649.6
UniGeneⁱ	Cel.30602

Genome annotation databases

EnsemblMetazoaⁱ	C15C7.7; C15C7.7; WBGene00015793
GeneIDⁱ	180607
KEGGⁱ	cel:CELE_C15C7.7
UCSC genome browser More...UCSCⁱ	C15C7.7 c. elegans

Similar proteinsⁱ

90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
Q18014	Uncharacterized protein pofut1		CAEEL		381	UniRef90_Q18014
UPI000225A88E		CAEEL		362

Protein	Similar proteins		Species	Score	Length	Source
Q18014	Uncharacterized protein pofut1		CAEEL		381	UniRef50_Q18014
Uncharacterized protein		Caenorhabditis tropicalis		345
Uncharacterized protein		Ancylostoma ceylanicum		381
Uncharacterized protein		PARTI		345
Uncharacterized protein		HAEPC		385
+3

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	FO080550 Genomic DNA Translation: CCD64591.1
RefSeqⁱ	NP_741744.2, NM_171649.6
UniGeneⁱ	Cel.30602

3D structure databases

Select the link destinations: Protein Data Bank Europe More...PDBeⁱ Protein Data Bank RCSB More...RCSB PDBⁱ Protein Data Bank Japan More...PDBjⁱ Links Updated	PDB entry	Method	Resolution (Å)	Chain	Positions	PDBsum
	3ZY2	X-ray	1.54	A	26-383	[»]
	3ZY3	X-ray	1.86	A/B	26-383	[»]
	3ZY4	X-ray	1.74	A	26-383	[»]
	3ZY5	X-ray	1.96	A	26-383	[»]
	3ZY6	X-ray	1.91	A	26-383	[»]
ProteinModelPortalⁱ	Q18014
SMRⁱ	Q18014
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

STRINGⁱ	6239.C15C7.7

STRINGⁱ

6239.C15C7.7

Protein family/group databases

CAZyⁱ	GT65 Glycosyltransferase Family 65

CAZyⁱ

GT65 Glycosyltransferase Family 65

Proteomic databases

EPDⁱ	Q18014
PaxDbⁱ	Q18014
PeptideAtlasⁱ	Q18014
PRIDEⁱ	Q18014

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

EnsemblMetazoaⁱ	C15C7.7; C15C7.7; WBGene00015793
GeneIDⁱ	180607
KEGGⁱ	cel:CELE_C15C7.7
UCSCⁱ	C15C7.7 c. elegans

Organism-specific databases

CTDⁱ	180607
WormBaseⁱ	C15C7.7 ; CE36786 ; WBGene00015793

Phylogenomic databases

eggNOGⁱ	KOG3202 Eukaryota KOG3849 Eukaryota ENOG410Y3JQ LUCA
GeneTreeⁱ	ENSGT00390000015634
HOGENOMⁱ	HOG000231651
InParanoidⁱ	Q18014
KOⁱ	K03691
OMAⁱ	GQSNHFI
OrthoDBⁱ	EOG091G048B
PhylomeDBⁱ	Q18014

Enzyme and pathway databases

UniPathwayⁱ	UPA00378
BRENDAⁱ	2.4.1.221 1045

Miscellaneous databases

EvolutionaryTraceⁱ	Q18014
Protein Ontology More...PROⁱ	PR:Q18014

Gene expression databases

Bgeeⁱ	WBGene00015793 Expressed in 5 organ(s), highest expression level in material anatomical entity

Bgeeⁱ

WBGene00015793 Expressed in 5 organ(s), highest expression level in material anatomical entity

Family and domain databases

InterProⁱ	View protein in InterPro IPR019378 GDP-Fuc_O-FucTrfase IPR039922 POFUT1
PANTHERⁱ	PTHR21420 PTHR21420, 1 hit
Pfamⁱ	View protein in Pfam PF10250 O-FucT, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	OFUT1_CAEEL
Accessionⁱ	Q18014Primary (citable) accession number: Q18014
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	October 19, 2002
	Last sequence update:	February 1, 2005
	Last modified:	November 7, 2018
	This is version 113 of the entry and version 5 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Caenorhabditis annotation project

Miscellaneousⁱ

Keywords - Technical termⁱ

3D-structure, Complete proteome, Reference proteome

Documents

SIMILARITY comments
Index of protein domains and families
Caenorhabditis elegans
Caenorhabditis elegans: entries, gene names and cross-references to WormBase
PATHWAY comments
Index of metabolic and biosynthesis pathways
PDB cross-references
Index of Protein Data Bank (PDB) cross-references

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - Q18014 (OFUT1_CAEEL)

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GDP-fucose protein O-fucosyltransferase 1

C15C7.7

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Miscellaneous

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: protein glycosylation

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

Protein family/group databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Endoplasmic reticulum

Endoplasmic reticulum

Keywords - Cellular componenti

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Keywords - PTMi

Proteomic databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

3D structure databases

Miscellaneous databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Region

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Keywords - Domaini

Phylogenomic databases

Family and domain databases

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Documents

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Functionⁱ

Pathwayⁱ: protein glycosylation

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Expressionⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Keywords - Domainⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ