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Protein

GDP-fucose protein O-fucosyltransferase 1

Gene

C15C7.7

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the reaction that attaches fucose through an O-glycosidic linkage to a conserved serine or threonine residue in EGF domains.1 Publication

Miscellaneous

Manganese is bound to the substrate GDP-fucose, but is not required for enzyme activity.1 Publication

Catalytic activityi

Transfers an alpha-L-fucosyl residue from GDP-beta-L-fucose to the serine hydroxy group of a protein acceptor.1 Publication

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

GO - Molecular functioni

  • fucosyltransferase activity Source: UniProtKB
  • peptide-O-fucosyltransferase activity Source: WormBase

GO - Biological processi

Keywordsi

Molecular functionGlycosyltransferase, Transferase
Biological processCarbohydrate metabolism, Fucose metabolism, Notch signaling pathway
LigandManganese

Enzyme and pathway databases

BRENDAi2.4.1.221 1045
UniPathwayi
UPA00378

Protein family/group databases

CAZyiGT65 Glycosyltransferase Family 65

Names & Taxonomyi

Protein namesi
Recommended name:
GDP-fucose protein O-fucosyltransferase 1 (EC:2.4.1.221)
Alternative name(s):
Peptide-O-fucosyltransferase 1
Short name:
O-FucT-1
Gene namesi
ORF Names:C15C7.7
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditinaRhabditomorphaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome X

Organism-specific databases

WormBaseiC15C7.7 ; CE36786 ; WBGene00015793

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Endoplasmic reticulum

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi43N → A: Reduces enzyme activity by over 90%. 1 Publication1
Mutagenesisi240R → A or K: Abolishes enzyme activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 21Sequence analysisAdd BLAST21
ChainiPRO_000001215222 – 389GDP-fucose protein O-fucosyltransferase 1Add BLAST368

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi24N-linked (GlcNAc...) asparagineCurated1
Disulfide bondi35 ↔ 371 Publication
Disulfide bondi119 ↔ 1351 Publication
Disulfide bondi249 ↔ 2811 Publication
Disulfide bondi266 ↔ 3531 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

EPDiQ18014
PaxDbiQ18014
PeptideAtlasiQ18014
PRIDEiQ18014

Expressioni

Gene expression databases

BgeeiWBGene00015793 Expressed in 5 organ(s), highest expression level in material anatomical entity

Interactioni

Subunit structurei

Monomer.1 Publication

Protein-protein interaction databases

STRINGi6239.C15C7.7

Structurei

Secondary structure

1389
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ18014
SMRiQ18014
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ18014

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni40 – 43Substrate bindingCombined sources4
Regioni238 – 240Substrate bindingCombined sources3
Regioni356 – 357Substrate bindingCombined sources1 Publication2

Sequence similaritiesi

Belongs to the glycosyltransferase 65 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG3202 Eukaryota
KOG3849 Eukaryota
ENOG410Y3JQ LUCA
GeneTreeiENSGT00390000015634
HOGENOMiHOG000231651
InParanoidiQ18014
KOiK03691
OMAiGQSNHFI
OrthoDBiEOG091G048B
PhylomeDBiQ18014

Family and domain databases

InterProiView protein in InterPro
IPR019378 GDP-Fuc_O-FucTrfase
IPR039922 POFUT1
PANTHERiPTHR21420 PTHR21420, 1 hit
PfamiView protein in Pfam
PF10250 O-FucT, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q18014-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MRVSKVLTLA SFISVCSYSE AKSNETDPNG YIVFCPCMGR FGNQVDQFLG
60 70 80 90 100
VLAFAKALDR TLVLPNFIEF KHPETKMIPF EFLFQVGTVA KYTRVVTMQE
110 120 130 140 150
FTKKIMPTVW PPEKRKAFCW TPRQAIYDKS AEPGCHSKEG NPFGPYWDQI
160 170 180 190 200
DVSFVGDEYF GDIPGGFDLN QMGSRKKWLE KFPSEEYPVL AFSSAPAPFP
210 220 230 240 250
SKGKVWSIQK YLRWSSRITE QAKKFISANL AKPFVAVHLR NDADWVRVCE
260 270 280 290 300
HIDTTTNRPL FASEQCLGEG HHLGTLTKEI CSPSKQQILE QIVEKVGSIG
310 320 330 340 350
AKSVFVASDK DHMIDEINEA LKPYEIEAHR QEPDDMYTSL AIMGRADLFV
360 370 380
GNCVSTFSHI VKRERDHAGQ SPRPSAFFGI RAVKRHIDL
Length:389
Mass (Da):44,051
Last modified:February 1, 2005 - v5
Checksum:i0C84F202AA184554
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FO080550 Genomic DNA Translation: CCD64591.1
RefSeqiNP_741744.2, NM_171649.6
UniGeneiCel.30602

Genome annotation databases

EnsemblMetazoaiC15C7.7; C15C7.7; WBGene00015793
GeneIDi180607
KEGGicel:CELE_C15C7.7
UCSCiC15C7.7 c. elegans

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FO080550 Genomic DNA Translation: CCD64591.1
RefSeqiNP_741744.2, NM_171649.6
UniGeneiCel.30602

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3ZY2X-ray1.54A26-383[»]
3ZY3X-ray1.86A/B26-383[»]
3ZY4X-ray1.74A26-383[»]
3ZY5X-ray1.96A26-383[»]
3ZY6X-ray1.91A26-383[»]
ProteinModelPortaliQ18014
SMRiQ18014
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi6239.C15C7.7

Protein family/group databases

CAZyiGT65 Glycosyltransferase Family 65

Proteomic databases

EPDiQ18014
PaxDbiQ18014
PeptideAtlasiQ18014
PRIDEiQ18014

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiC15C7.7; C15C7.7; WBGene00015793
GeneIDi180607
KEGGicel:CELE_C15C7.7
UCSCiC15C7.7 c. elegans

Organism-specific databases

CTDi180607
WormBaseiC15C7.7 ; CE36786 ; WBGene00015793

Phylogenomic databases

eggNOGiKOG3202 Eukaryota
KOG3849 Eukaryota
ENOG410Y3JQ LUCA
GeneTreeiENSGT00390000015634
HOGENOMiHOG000231651
InParanoidiQ18014
KOiK03691
OMAiGQSNHFI
OrthoDBiEOG091G048B
PhylomeDBiQ18014

Enzyme and pathway databases

UniPathwayi
UPA00378

BRENDAi2.4.1.221 1045

Miscellaneous databases

EvolutionaryTraceiQ18014
PROiPR:Q18014

Gene expression databases

BgeeiWBGene00015793 Expressed in 5 organ(s), highest expression level in material anatomical entity

Family and domain databases

InterProiView protein in InterPro
IPR019378 GDP-Fuc_O-FucTrfase
IPR039922 POFUT1
PANTHERiPTHR21420 PTHR21420, 1 hit
PfamiView protein in Pfam
PF10250 O-FucT, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiOFUT1_CAEEL
AccessioniPrimary (citable) accession number: Q18014
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 19, 2002
Last sequence update: February 1, 2005
Last modified: November 7, 2018
This is version 113 of the entry and version 5 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  4. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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