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UniProtKB - Q18014 (OFUT1_CAEEL)
Protein
GDP-fucose protein O-fucosyltransferase 1
Gene
C15C7.7
Organism
Caenorhabditis elegans
Status
Functioni
Catalyzes the reaction that attaches fucose through an O-glycosidic linkage to a conserved serine or threonine residue in EGF domains.
1 PublicationMiscellaneous
Manganese is bound to the substrate GDP-fucose, but is not required for enzyme activity.1 Publication
Catalytic activityi
- Transfers an alpha-L-fucosyl residue from GDP-beta-L-fucose to the serine hydroxy group of a protein acceptor.1 Publication EC:2.4.1.221
: protein glycosylation Pathwayi
This protein is involved in the pathway protein glycosylation, which is part of Protein modification.View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.
GO - Molecular functioni
- fucosyltransferase activity Source: UniProtKB
- peptide-O-fucosyltransferase activity Source: WormBase
GO - Biological processi
- fucose metabolic process Source: UniProtKB-KW
- Notch signaling pathway Source: UniProtKB-KW
- protein O-linked fucosylation Source: WormBase
- protein O-linked glycosylation Source: UniProtKB
- regulation of Notch signaling pathway Source: GO_Central
Keywordsi
Molecular function | Glycosyltransferase, Transferase |
Biological process | Carbohydrate metabolism, Fucose metabolism, Notch signaling pathway |
Ligand | Manganese |
Enzyme and pathway databases
BRENDAi | 2.4.1.221, 1045 |
UniPathwayi | UPA00378 |
Protein family/group databases
CAZyi | GT65, Glycosyltransferase Family 65 |
Names & Taxonomyi
Protein namesi | Recommended name: GDP-fucose protein O-fucosyltransferase 1 (EC:2.4.1.221)Alternative name(s): Peptide-O-fucosyltransferase 1 Short name: O-FucT-1 |
Gene namesi | ORF Names:C15C7.7 |
Organismi | Caenorhabditis elegans |
Taxonomic identifieri | 6239 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Ecdysozoa › Nematoda › Chromadorea › Rhabditida › Rhabditina › Rhabditomorpha › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis |
Proteomesi |
|
Organism-specific databases
WormBasei | C15C7.7 ; CE36786 ; WBGene00015793 |
Subcellular locationi
Endoplasmic reticulum
- Endoplasmic reticulum By similarity
Endoplasmic reticulum
- endoplasmic reticulum Source: WormBase
Keywords - Cellular componenti
Endoplasmic reticulumPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 43 | N → A: Reduces enzyme activity by over 90%. 1 Publication | 1 | |
Mutagenesisi | 240 | R → A or K: Abolishes enzyme activity. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 21 | Sequence analysisAdd BLAST | 21 | |
ChainiPRO_0000012152 | 22 – 389 | GDP-fucose protein O-fucosyltransferase 1Add BLAST | 368 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 24 | N-linked (GlcNAc...) asparagineCurated | 1 | |
Disulfide bondi | 35 ↔ 37 | 1 Publication | ||
Disulfide bondi | 119 ↔ 135 | 1 Publication | ||
Disulfide bondi | 249 ↔ 281 | 1 Publication | ||
Disulfide bondi | 266 ↔ 353 | 1 Publication |
Keywords - PTMi
Disulfide bond, GlycoproteinProteomic databases
EPDi | Q18014 |
PaxDbi | Q18014 |
PeptideAtlasi | Q18014 |
Expressioni
Gene expression databases
Bgeei | WBGene00015793, Expressed in material anatomical entity and 5 other tissues |
Interactioni
Subunit structurei
Monomer.
1 PublicationProtein-protein interaction databases
BioGRIDi | 45548, 1 interactor |
STRINGi | 6239.C15C7.7 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q18014 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q18014 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 40 – 43 | Substrate bindingCombined sources | 4 | |
Regioni | 238 – 240 | Substrate bindingCombined sources | 3 | |
Regioni | 356 – 357 | Substrate bindingCombined sources1 Publication | 2 |
Sequence similaritiesi
Belongs to the glycosyltransferase 65 family.Curated
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | KOG3202, Eukaryota KOG3849, Eukaryota |
GeneTreei | ENSGT00390000015634 |
HOGENOMi | CLU_039551_0_0_1 |
InParanoidi | Q18014 |
OMAi | YPELCMP |
OrthoDBi | 1127619at2759 |
PhylomeDBi | Q18014 |
Family and domain databases
CDDi | cd11302, O-FucT-1, 1 hit |
InterProi | View protein in InterPro IPR019378, GDP-Fuc_O-FucTrfase IPR039922, POFUT1 |
PANTHERi | PTHR21420, PTHR21420, 1 hit |
Pfami | View protein in Pfam PF10250, O-FucT, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
Q18014-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MRVSKVLTLA SFISVCSYSE AKSNETDPNG YIVFCPCMGR FGNQVDQFLG
60 70 80 90 100
VLAFAKALDR TLVLPNFIEF KHPETKMIPF EFLFQVGTVA KYTRVVTMQE
110 120 130 140 150
FTKKIMPTVW PPEKRKAFCW TPRQAIYDKS AEPGCHSKEG NPFGPYWDQI
160 170 180 190 200
DVSFVGDEYF GDIPGGFDLN QMGSRKKWLE KFPSEEYPVL AFSSAPAPFP
210 220 230 240 250
SKGKVWSIQK YLRWSSRITE QAKKFISANL AKPFVAVHLR NDADWVRVCE
260 270 280 290 300
HIDTTTNRPL FASEQCLGEG HHLGTLTKEI CSPSKQQILE QIVEKVGSIG
310 320 330 340 350
AKSVFVASDK DHMIDEINEA LKPYEIEAHR QEPDDMYTSL AIMGRADLFV
360 370 380
GNCVSTFSHI VKRERDHAGQ SPRPSAFFGI RAVKRHIDL
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | FO080550 Genomic DNA Translation: CCD64591.1 |
RefSeqi | NP_741744.2, NM_171649.6 |
Genome annotation databases
EnsemblMetazoai | C15C7.7.1; C15C7.7.1; WBGene00015793 |
GeneIDi | 180607 |
KEGGi | cel:CELE_C15C7.7 |
UCSCi | C15C7.7, c. elegans |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | FO080550 Genomic DNA Translation: CCD64591.1 |
RefSeqi | NP_741744.2, NM_171649.6 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3ZY2 | X-ray | 1.54 | A | 26-383 | [»] | |
3ZY3 | X-ray | 1.86 | A/B | 26-383 | [»] | |
3ZY4 | X-ray | 1.74 | A | 26-383 | [»] | |
3ZY5 | X-ray | 1.96 | A | 26-383 | [»] | |
3ZY6 | X-ray | 1.91 | A | 26-383 | [»] | |
SMRi | Q18014 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 45548, 1 interactor |
STRINGi | 6239.C15C7.7 |
Protein family/group databases
CAZyi | GT65, Glycosyltransferase Family 65 |
Proteomic databases
EPDi | Q18014 |
PaxDbi | Q18014 |
PeptideAtlasi | Q18014 |
Genome annotation databases
EnsemblMetazoai | C15C7.7.1; C15C7.7.1; WBGene00015793 |
GeneIDi | 180607 |
KEGGi | cel:CELE_C15C7.7 |
UCSCi | C15C7.7, c. elegans |
Organism-specific databases
CTDi | 180607 |
WormBasei | C15C7.7 ; CE36786 ; WBGene00015793 |
Phylogenomic databases
eggNOGi | KOG3202, Eukaryota KOG3849, Eukaryota |
GeneTreei | ENSGT00390000015634 |
HOGENOMi | CLU_039551_0_0_1 |
InParanoidi | Q18014 |
OMAi | YPELCMP |
OrthoDBi | 1127619at2759 |
PhylomeDBi | Q18014 |
Enzyme and pathway databases
UniPathwayi | UPA00378 |
BRENDAi | 2.4.1.221, 1045 |
Miscellaneous databases
EvolutionaryTracei | Q18014 |
PROi | PR:Q18014 |
Gene expression databases
Bgeei | WBGene00015793, Expressed in material anatomical entity and 5 other tissues |
Family and domain databases
CDDi | cd11302, O-FucT-1, 1 hit |
InterProi | View protein in InterPro IPR019378, GDP-Fuc_O-FucTrfase IPR039922, POFUT1 |
PANTHERi | PTHR21420, PTHR21420, 1 hit |
Pfami | View protein in Pfam PF10250, O-FucT, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | OFUT1_CAEEL | |
Accessioni | Q18014Primary (citable) accession number: Q18014 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 19, 2002 |
Last sequence update: | February 1, 2005 | |
Last modified: | February 23, 2022 | |
This is version 128 of the entry and version 5 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Caenorhabditis annotation project |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Caenorhabditis elegans
Caenorhabditis elegans: entries, gene names and cross-references to WormBase - PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families