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UniProtKB - Q17EN4 (HOT_AEDAE)
Protein
Probable hydroxyacid-oxoacid transhydrogenase, mitochondrial
Gene
AAEL003729
Organism
Aedes aegypti (Yellowfever mosquito) (Culex aegypti)
Status
Functioni
Catalyzes the cofactor-independent reversible oxidation of gamma-hydroxybutyrate (GHB) to succinic semialdehyde (SSA) coupled to reduction of 2-ketoglutarate (2-KG) to D-2-hydroxyglutarate (D-2-HG). L-3-hydroxybutyrate (L-3-OHB) is also a substrate for HOT when using 2-KG as hydrogen acceptor, resulting in the formation of D-2-HG (By similarity).
By similarityCatalytic activityi
GO - Molecular functioni
- alcohol dehydrogenase (NAD+) activity Source: InterPro
- hydroxyacid-oxoacid transhydrogenase activity Source: UniProtKB
- metal ion binding Source: InterPro
GO - Biological processi
- glutamate catabolic process via 2-oxoglutarate Source: UniProtKB
Keywordsi
Molecular function | Oxidoreductase |
Names & Taxonomyi
Protein namesi | Recommended name: Probable hydroxyacid-oxoacid transhydrogenase, mitochondrial (EC:1.1.99.24)Short name: HOT |
Gene namesi | ORF Names:AAEL003729 |
Organismi | Aedes aegypti (Yellowfever mosquito) (Culex aegypti) |
Taxonomic identifieri | 7159 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Ecdysozoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Nematocera › Culicoidea › Culicidae › Culicinae › Aedini › Aedes › Stegomyia |
Proteomesi |
|
Organism-specific databases
VEuPathDBi | VectorBase:AAEL003729 |
Subcellular locationi
Mitochondrion
- Mitochondrion By similarity
Mitochondrion
- mitochondrion Source: UniProtKB
Keywords - Cellular componenti
MitochondrionPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transit peptidei | 1 – ? | MitochondrionSequence analysis | ||
ChainiPRO_0000323003 | ? – 462 | Probable hydroxyacid-oxoacid transhydrogenase, mitochondrial |
Proteomic databases
PRIDEi | Q17EN4 |
Family & Domainsi
Sequence similaritiesi
Belongs to the iron-containing alcohol dehydrogenase family. Hydroxyacid-oxoacid transhydrogenase subfamily.Curated
Keywords - Domaini
Transit peptidePhylogenomic databases
eggNOGi | KOG3857, Eukaryota |
InParanoidi | Q17EN4 |
OMAi | IRFGPGC |
OrthoDBi | 776004at2759 |
PhylomeDBi | Q17EN4 |
Family and domain databases
CDDi | cd08190, HOT, 1 hit |
InterProi | View protein in InterPro IPR001670, ADH_Fe/GldA IPR039697, Alcohol_dehydrogenase_Fe IPR042157, HOT |
PANTHERi | PTHR11496, PTHR11496, 1 hit |
Pfami | View protein in Pfam PF00465, Fe-ADH, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
Q17EN4-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MMSKARISSL MKVISSGSCS CPAHSRSSTG GLNVSSENEY AFEMSSSTIR
60 70 80 90 100
YGPGVSKELG HDLQNLNAKN VCIVTDRNVA KLNSVKVAFD SLTRCGIQYQ
110 120 130 140 150
VYDETRVEPT DQSLLHAAEY ARQNKFDSFV AIGGGSVIDT CKVANLFSAD
160 170 180 190 200
REAEFLDYVN APIGKAKEVN VKLKPLIAVP TTAGTGSETT GVVIFDYKPL
210 220 230 240 250
HAKTGISSKY LRPQLGLIDP LHTLSQPEKV AAYCGFDVFC HALESFTAIP
260 270 280 290 300
YTERGSAPMN PNLRPPYQGS NPISDVWARF ALKIIRENFI SAVFNQDDLK
310 320 330 340 350
ARSNMHLAST MAGVGFGNAG VHLCHGLSYP ISGHVKKFVP DGYSGDHPII
360 370 380 390 400
PHGLSVVMTA PAVFKFTAAS CPERHLEAAE LLGANVSKAN RNDAGSILSD
410 420 430 440 450
VVRQYMYKLK IENGLDSLGF TRDDIQSLVK GTLPQERITK LAPRAQTEED
460
LASLFENSMT VY
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A6I8T8C0 | A0A6I8T8C0_AEDAE | Hydroxyacid-oxoacid transhydrogenas... | 5578877 | 462 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CH477280 Genomic DNA Translation: EAT44958.1 |
RefSeqi | XP_001664064.1, XM_001664014.1 |
Genome annotation databases
GeneIDi | 5578877 |
KEGGi | aag:5578877 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CH477280 Genomic DNA Translation: EAT44958.1 |
RefSeqi | XP_001664064.1, XM_001664014.1 |
3D structure databases
AlphaFoldDBi | Q17EN4 |
SMRi | Q17EN4 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 7159.AAEL003729-PB |
Proteomic databases
PRIDEi | Q17EN4 |
Genome annotation databases
GeneIDi | 5578877 |
KEGGi | aag:5578877 |
Organism-specific databases
VEuPathDBi | VectorBase:AAEL003729 |
Phylogenomic databases
eggNOGi | KOG3857, Eukaryota |
InParanoidi | Q17EN4 |
OMAi | IRFGPGC |
OrthoDBi | 776004at2759 |
PhylomeDBi | Q17EN4 |
Family and domain databases
CDDi | cd08190, HOT, 1 hit |
InterProi | View protein in InterPro IPR001670, ADH_Fe/GldA IPR039697, Alcohol_dehydrogenase_Fe IPR042157, HOT |
PANTHERi | PTHR11496, PTHR11496, 1 hit |
Pfami | View protein in Pfam PF00465, Fe-ADH, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | HOT_AEDAE | |
Accessioni | Q17EN4Primary (citable) accession number: Q17EN4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 18, 2008 |
Last sequence update: | July 25, 2006 | |
Last modified: | May 25, 2022 | |
This is version 92 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families