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Protein

Thioredoxin reductase 1

Gene

trxr-1

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Together with glutathione reductase gsr-1, required for the reduction of disulfide groups in the cuticle during molting.1 Publication

Miscellaneous

The active site is a redox-active disulfide bond.

Catalytic activityi

Thioredoxin + NADP+ = thioredoxin disulfide + NADPH.1 Publication

Cofactori

FADBy similarityNote: Binds 1 FAD per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei639Proton acceptorBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi202 – 219FADBy similarityAdd BLAST18

GO - Molecular functioni

GO - Biological processi

  • cell redox homeostasis Source: GO_Central
  • ecdysis, collagen and cuticulin-based cuticle Source: WormBase
  • response to oxygen radical Source: GO_Central

Keywordsi

Molecular functionOxidoreductase
LigandFAD, Flavoprotein, NADP

Enzyme and pathway databases

ReactomeiR-CEL-5263617 Metabolism of ingested MeSeO2H into MeSeH

Names & Taxonomyi

Protein namesi
Recommended name:
Thioredoxin reductase 1 (EC:1.8.1.91 Publication)
Short name:
TR-Se
Short name:
TRR
Gene namesi
Name:trxr-1
ORF Names:C06G3.7
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome IV

Organism-specific databases

WormBaseiC06G3.7a ; CE34250 ; WBGene00015553 ; trxr-1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

No obvious phenotype. RNAi-mediated knockdown of gsr-1 in mutants lacking trxr-1 causes an arrest during larval molting characterized by a partial detachment of the old cuticle and an impaired ability to reduce cuticle components. Also results in growth arrest during the postdauer molt.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi666U → C: Loss of activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000679801 – 667Thioredoxin reductase 1Add BLAST667

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi219 ↔ 224Redox-activeBy similarity
Cross-linki665 ↔ 666Cysteinyl-selenocysteine (Cys-Sec)By similarity

Post-translational modificationi

Contains a selenide-sulfide bond between Cys-665 and Sec-666. This bond is speculated to serve as redox-active pair (By similarity).By similarity

Keywords - PTMi

Disulfide bond

Proteomic databases

EPDiQ17745
PaxDbiQ17745
PeptideAtlasiQ17745
PRIDEiQ17745

PTM databases

iPTMnetiQ17745

Expressioni

Tissue specificityi

Expressed in intestine, pharynx, seam cells, hypodermis, rectal epithelial cells, neurons in the nerve ring and amphid sheath cells.1 Publication

Gene expression databases

BgeeiWBGene00015553 Expressed in 3 organ(s), highest expression level in adult organism

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi6239.C06G3.7

Structurei

3D structure databases

ProteinModelPortaliQ17745
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Redox-active center

Phylogenomic databases

eggNOGiKOG0405 Eukaryota
COG1249 LUCA
InParanoidiQ17745
KOiK22182
OMAiNTCYAKI
OrthoDBiEOG091G03IU
PhylomeDBiQ17745

Family and domain databases

Gene3Di3.30.390.30, 1 hit
3.50.50.60, 3 hits
InterProiView protein in InterPro
IPR036188 FAD/NAD-bd_sf
IPR023753 FAD/NAD-binding_dom
IPR016156 FAD/NAD-linked_Rdtase_dimer_sf
IPR004099 Pyr_nucl-diS_OxRdtase_dimer
IPR012999 Pyr_OxRdtase_I_AS
IPR006338 Thioredoxin/glutathione_Rdtase
PfamiView protein in Pfam
PF07992 Pyr_redox_2, 1 hit
PF02852 Pyr_redox_dim, 1 hit
SUPFAMiSSF51905 SSF51905, 1 hit
SSF55424 SSF55424, 1 hit
TIGRFAMsiTIGR01438 TGR, 1 hit
PROSITEiView protein in PROSITE
PS00076 PYRIDINE_REDOX_1, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

Q17745-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MKSLTELFGC FKRQPRQQEA SSPANPHVSD TLSMGVAASG MPPPKRPAPA
60 70 80 90 100
ESPTLPGETL VDAPGIPLKE ALKEAANSKI VIFYNSSDEE KQLVEFETYL
110 120 130 140 150
NSLKEPADAE KPLEIPEIKK LQVSRASQKV IQYLTLHTSW PLMYIKGNAV
160 170 180 190 200
GGLKELKALK QDYLKEWLRD HTYDLIVIGG GSGGLAAAKE ASRLGKKVAC
210 220 230 240 250
LDFVKPSPQG TSWGLGGTCV NVGCIPKKLM HQASLLGHSI HDAKKYGWKL
260 270 280 290 300
PEGKVEHQWN HLRDSVQDHI ASLNWGYRVQ LREKTVTYIN SYGEFTGPFE
310 320 330 340 350
ISATNKKKKV EKLTADRFLI STGLRPKYPE IPGVKEYTIT SDDLFQLPYS
360 370 380 390 400
PGKTLCVGAS YVSLECAGFL HGFGFDVTVM VRSILLRGFD QDMAERIRKH
410 420 430 440 450
MIAYGMKFEA GVPTRIEQID EKTDEKAGKY RVFWPKKNEE TGEMQEVSEE
460 470 480 490 500
YNTILMAIGR EAVTDDVGLT TIGVERAKSK KVLGRREQST TIPWVYAIGD
510 520 530 540 550
VLEGTPELTP VAIQAGRVLM RRIFDGANEL TEYDQIPTTV FTPLEYGCCG
560 570 580 590 600
LSEEDAMMKY GKDNIIIYHN VFNPLEYTIS ERMDKDHCYL KMICLRNEEE
610 620 630 640 650
KVVGFHILTP NAGEVTQGFG IALKLAAKKA DFDRLIGIHP TVAENFTTLT
660
LEKKEGDEEL QASGCUG
Length:667
Mass (Da):74,263
Last modified:February 26, 2008 - v3
Checksum:i58B86D4274E4FA77
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
V6CLN2V6CLN2_CAEEL
Thioredoxin reductase 1
trxr-1 C06G3.7, CELE_C06G3.7
634Annotation score:

Non-standard residue

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Non-standard residuei666Selenocysteine1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF148217 mRNA Translation: AAD41826.1
FO080396 Genomic DNA Translation: CCD83555.1
AF162693 mRNA Translation: AAD46625.1
PIRiT30091
RefSeqiNP_001294239.1, NM_001307310.1
UniGeneiCel.18219

Genome annotation databases

EnsemblMetazoaiC06G3.7a; C06G3.7a; WBGene00015553
GeneIDi177466
KEGGicel:CELE_C06G3.7
UCSCiC06G3.7 c. elegans

Keywords - Coding sequence diversityi

Selenocysteine

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF148217 mRNA Translation: AAD41826.1
FO080396 Genomic DNA Translation: CCD83555.1
AF162693 mRNA Translation: AAD46625.1
PIRiT30091
RefSeqiNP_001294239.1, NM_001307310.1
UniGeneiCel.18219

3D structure databases

ProteinModelPortaliQ17745
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi6239.C06G3.7

PTM databases

iPTMnetiQ17745

Proteomic databases

EPDiQ17745
PaxDbiQ17745
PeptideAtlasiQ17745
PRIDEiQ17745

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiC06G3.7a; C06G3.7a; WBGene00015553
GeneIDi177466
KEGGicel:CELE_C06G3.7
UCSCiC06G3.7 c. elegans

Organism-specific databases

CTDi31760
WormBaseiC06G3.7a ; CE34250 ; WBGene00015553 ; trxr-1

Phylogenomic databases

eggNOGiKOG0405 Eukaryota
COG1249 LUCA
InParanoidiQ17745
KOiK22182
OMAiNTCYAKI
OrthoDBiEOG091G03IU
PhylomeDBiQ17745

Enzyme and pathway databases

ReactomeiR-CEL-5263617 Metabolism of ingested MeSeO2H into MeSeH

Miscellaneous databases

PROiPR:Q17745

Gene expression databases

BgeeiWBGene00015553 Expressed in 3 organ(s), highest expression level in adult organism

Family and domain databases

Gene3Di3.30.390.30, 1 hit
3.50.50.60, 3 hits
InterProiView protein in InterPro
IPR036188 FAD/NAD-bd_sf
IPR023753 FAD/NAD-binding_dom
IPR016156 FAD/NAD-linked_Rdtase_dimer_sf
IPR004099 Pyr_nucl-diS_OxRdtase_dimer
IPR012999 Pyr_OxRdtase_I_AS
IPR006338 Thioredoxin/glutathione_Rdtase
PfamiView protein in Pfam
PF07992 Pyr_redox_2, 1 hit
PF02852 Pyr_redox_dim, 1 hit
SUPFAMiSSF51905 SSF51905, 1 hit
SSF55424 SSF55424, 1 hit
TIGRFAMsiTIGR01438 TGR, 1 hit
PROSITEiView protein in PROSITE
PS00076 PYRIDINE_REDOX_1, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiTRXR1_CAEEL
AccessioniPrimary (citable) accession number: Q17745
Secondary accession number(s): Q9N2K1, Q9NJH3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 26, 2008
Last modified: October 10, 2018
This is version 166 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
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