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Protein

Mitogen-activated protein kinase pmk-1

Gene

pmk-1

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Serine/threonine kinase which responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating downstream targets. Phosphorylates snk-1 which probably regulates skn-1 nuclear translocation in response to oxidative stress (PubMed:16166371). Probably by activating skn-1, involved in the up-regulation of gcs-1 and glutathione-S-transferase gst-4 expression upon bacteria infection (PubMed:22216003). Up-regulates expression of gcs-1 in intestinal cells upon arsenite treatment (PubMed:16166371). Functions downstream of the MAPKK sek-1 and the MAPKKK nsy-1 as the MAP kinase which regulates pathogen resistance and responses to oxidative stress (PubMed:11703092, PubMed:12142542, PubMed:18394898, PubMed:16166371). Required for expression of antimicrobial peptide nlp-29 in response to fungal infection or physical injury (PubMed:18394898). Involved in resistance to the nematotoxic C.cinerea galectin Cgl2 (PubMed:20062796). May play a redundant role with other MAP kinases in susceptibility to anoxia, downstream of tir-1/nsy-1 (PubMed:21212236). Phosphorylates transcription factor rnt-1 during oxidative stress which results in rnt-1 stabilization in the intestine (PubMed:22308034). Probably downstream of nsy-1 and sek-1, involved in germline apoptosis induced by heavy metals, such as Cu2+ (PubMed:19497412).9 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.3 Publications

Cofactori

Mg2+2 Publications, Mn2+1 PublicationNote: Divalent cations such as magnesium or manganese.1 Publication

Enzyme regulationi

Activated by phosphorylation on threonine and tyrosine. Inhibited by pyridinyl-imidazole related compounds.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei64ATPPROSITE-ProRule annotation1
Active sitei179Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi41 – 49ATPPROSITE-ProRule annotation9

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • MAP kinase activity Source: UniProtKB
  • metal ion binding Source: UniProtKB-KW
  • protein kinase activity Source: WormBase
  • protein serine/threonine kinase activity Source: WormBase
  • transcription factor binding Source: WormBase

GO - Biological processi

  • behavioral response to nicotine Source: UniProtKB
  • defense response to fungus Source: UniProtKB
  • defense response to Gram-negative bacterium Source: UniProtKB
  • defense response to Gram-positive bacterium Source: UniProtKB
  • determination of adult lifespan Source: UniProtKB
  • hyperosmotic response Source: WormBase
  • innate immune response Source: UniProtKB
  • intracellular signal transduction Source: UniProtKB
  • MAPK cascade Source: WormBase
  • negative regulation of neuron death Source: ParkinsonsUK-UCL
  • p38MAPK cascade Source: WormBase
  • peptidyl-serine phosphorylation Source: CACAO
  • positive regulation of antimicrobial peptide production Source: UniProtKB
  • positive regulation of defense response to bacterium Source: UniProtKB
  • positive regulation of protein localization to nucleus Source: WormBase
  • positive regulation of transcription by RNA polymerase II Source: WormBase
  • positive regulation of transcription from RNA polymerase II promoter involved in defense response to fungus Source: UniProtKB
  • positive regulation of transcription from RNA polymerase II promoter involved in defense response to Gram-negative bacterium Source: WormBase
  • programmed cell death Source: WormBase
  • protein phosphorylation Source: WormBase
  • regulation of defense response to fungus Source: UniProtKB
  • response to nematicide Source: UniProtKB
  • response to osmotic stress Source: UniProtKB
  • response to reactive oxygen species Source: WormBase
  • response to superoxide Source: WormBase

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
Biological processStress response
LigandATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-CEL-168638 NOD1/2 Signaling Pathway
R-CEL-171007 p38MAPK events
R-CEL-198753 ERK/MAPK targets
R-CEL-2559580 Oxidative Stress Induced Senescence
R-CEL-375170 CDO in myogenesis
R-CEL-418592 ADP signalling through P2Y purinoceptor 1
R-CEL-432142 Platelet sensitization by LDL
R-CEL-4420097 VEGFA-VEGFR2 Pathway
R-CEL-450302 activated TAK1 mediates p38 MAPK activation
R-CEL-6798695 Neutrophil degranulation
SignaLinkiQ17446

Names & Taxonomyi

Protein namesi
Recommended name:
Mitogen-activated protein kinase pmk-1 (EC:2.7.11.243 Publications)
Alternative name(s):
Stress-activated protein kinase pmk-1
p38 MAP kinase 1
Gene namesi
Name:pmk-1
ORF Names:B0218.3
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome IV

Organism-specific databases

WormBaseiB0218.3 ; CE06686 ; WBGene00004055 ; pmk-1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Pathology & Biotechi

Disruption phenotypei

Upon infection by P.aeruginosa or E.faecalis, RNAi-mediated knockdown results in a decrease in survival rate and in a reduced up-regulation of gst-4 and gcs-1 expression (PubMed:12142542, PubMed:22216003). Causes a severe reduction in rnt-1 accumulation in the intestine during oxidative stress mediated by paraquat (PubMed:22308034). Upon exposure to C.cinerea galectin Cgl2, adults show reduced survival and larvae do not develop (PubMed:20062796). Larval development is partially restored in a bre-1, fut-8, gly-13, galt-1 or ger-1 mutant background (PubMed:20062796).4 Publications

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi64K → R: Loss of kinase activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001863031 – 377Mitogen-activated protein kinase pmk-1Add BLAST377

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei191Phosphothreonine2 Publications1
Modified residuei193Phosphotyrosine2 Publications1

Post-translational modificationi

Dually phosphorylated on Thr-191 and Tyr-193, probably by sek-1, which activates the enzyme.3 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ17446
PaxDbiQ17446
PeptideAtlasiQ17446
PRIDEiQ17446

PTM databases

iPTMnetiQ17446

Expressioni

Gene expression databases

BgeeiWBGene00004055

Interactioni

GO - Molecular functioni

  • transcription factor binding Source: WormBase

Protein-protein interaction databases

BioGridi56238, 22 interactors
DIPiDIP-26892N
IntActiQ17446, 22 interactors
STRINGi6239.B0218.3

Structurei

3D structure databases

ProteinModelPortaliQ17446
SMRiQ17446
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini35 – 319Protein kinasePROSITE-ProRule annotationAdd BLAST285

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi191 – 193TXY3

Domaini

The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0660 Eukaryota
ENOG410XNY0 LUCA
GeneTreeiENSGT00550000074271
HOGENOMiHOG000233024
InParanoidiQ17446
KOiK04441
OMAiDVIDTIC
OrthoDBiEOG091G08QL
PhylomeDBiQ17446

Family and domain databases

InterProiView protein in InterPro
IPR011009 Kinase-like_dom_sf
IPR003527 MAP_kinase_CS
IPR008352 MAPK_p38-like
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
PfamiView protein in Pfam
PF00069 Pkinase, 1 hit
PRINTSiPR01773 P38MAPKINASE
SMARTiView protein in SMART
SM00220 S_TKc, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS01351 MAPK, 1 hit
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit

Sequencei

Sequence statusi: Complete.

Q17446-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFPQTTMDHI LHPTPREGYY VVELNRSVWV VPNYYINLTP IGTGAYGTVC
60 70 80 90 100
AAECTRSGTR VAIKKFNRPF QSIIHARRTY RELRLLRCMC HENIIDLLDV
110 120 130 140 150
FTPNENVNDI EDVYFVSMLM GADLSNILKI QRLNDDHIQF LVYQILRGLK
160 170 180 190 200
YIHSADIIHR DLKPSNIAVN EDCELKILDF GLARQTDSEM TGYVATRWYR
210 220 230 240 250
APEIMLNWMH YTQTVDVWSV GCILAELITG KTLFPGSDHI DQLTRIMSVT
260 270 280 290 300
GTPDEEFLKK ISSEEARNYI RNLPKMTRRD FKRLFAQATP QAIDLLEKML
310 320 330 340 350
HLDPDRRPTA KEAMEHEYLA AYHDETDEPI AEEMDLNDDV RADTIDEWKK
360 370
IIWEEISDFQ KNVAFADEEE DEEKMES
Length:377
Mass (Da):43,919
Last modified:November 1, 1996 - v1
Checksum:iF20BC395B38EFD03
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FO080124 Genomic DNA Translation: CCD61386.1
PIRiT29750
RefSeqiNP_501365.1, NM_068964.3
UniGeneiCel.12534

Genome annotation databases

EnsemblMetazoaiB0218.3; B0218.3; WBGene00004055
GeneIDi191743
KEGGicel:CELE_B0218.3
UCSCiB0218.3 c. elegans

Similar proteinsi

Entry informationi

Entry nameiPMK1_CAEEL
AccessioniPrimary (citable) accession number: Q17446
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 30, 2003
Last sequence update: November 1, 1996
Last modified: July 18, 2018
This is version 159 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families

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