UniProtKB - Q16790 (CAH9_HUMAN)
Protein
Carbonic anhydrase 9
Gene
CA9
Organism
Homo sapiens (Human)
Status
Functioni
Reversible hydration of carbon dioxide. Participates in pH regulation. May be involved in the control of cell proliferation and transformation. Appears to be a novel specific biomarker for a cervical neoplasia.1 Publication
Catalytic activityi
- EC:4.2.1.1
Cofactori
Zn2+1 Publication
Activity regulationi
Inhibited by coumarins, saccharin, sulfonamide derivatives such as acetazolamide (AZA) and Foscarnet (phosphonoformate trisodium salt).4 Publications
pH dependencei
Optimum pH is 6.5.1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 200 | Proton acceptorBy similarity | 1 | |
Metal bindingi | 226 | Zinc; catalytic1 Publication | 1 | |
Metal bindingi | 228 | Zinc; catalytic1 Publication | 1 | |
Metal bindingi | 251 | Zinc; catalytic1 Publication | 1 |
GO - Molecular functioni
- carbonate dehydratase activity Source: Reactome
- zinc ion binding Source: InterPro
GO - Biological processi
- bicarbonate transport Source: Reactome
- morphogenesis of an epithelium Source: Ensembl
- regulation of transcription from RNA polymerase II promoter in response to hypoxia Source: Reactome
- response to drug Source: Ensembl
- response to testosterone Source: Ensembl
- secretion Source: Ensembl
Keywordsi
Molecular function | Lyase |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
BRENDAi | 4.2.1.1 2681 |
Reactomei | R-HSA-1234158 Regulation of gene expression by Hypoxia-inducible Factor R-HSA-1475029 Reversible hydration of carbon dioxide |
SIGNORi | Q16790 |
Names & Taxonomyi
Protein namesi | Recommended name: Carbonic anhydrase 9 (EC:4.2.1.1)Alternative name(s): Carbonate dehydratase IX Carbonic anhydrase IX Short name: CA-IX Short name: CAIX Membrane antigen MN P54/58N Renal cell carcinoma-associated antigen G250 Short name: RCC-associated antigen G250 pMW1 |
Gene namesi | Name:CA9 Synonyms:G250, MN |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
EuPathDBi | HostDB:ENSG00000107159.12 |
HGNCi | HGNC:1383 CA9 |
MIMi | 603179 gene |
neXtProti | NX_Q16790 |
Subcellular locationi
Nucleus
Plasma membrane
- Cell membrane 1 Publication; Single-pass type I membrane protein 1 Publication
- microvillus membrane 1 Publication; Single-pass type I membrane protein 1 Publication
Note: Found on the surface microvilli and in the nucleus, particularly in nucleolus.
Nucleus
- nucleolus Source: UniProtKB-SubCell
Plasma Membrane
- basolateral plasma membrane Source: Ensembl
- microvillus membrane Source: UniProtKB-SubCell
- plasma membrane Source: UniProtKB
Other locations
- integral component of membrane Source: ProtInc
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 38 – 414 | ExtracellularAdd BLAST | 377 | |
Transmembranei | 415 – 435 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 436 – 459 | CytoplasmicAdd BLAST | 24 |
Keywords - Cellular componenti
Cell membrane, Cell projection, Membrane, NucleusPathology & Biotechi
Organism-specific databases
DisGeNETi | 768 |
OpenTargetsi | ENSG00000107159 |
PharmGKBi | PA25998 |
Chemistry databases
ChEMBLi | CHEMBL3594 |
DrugBanki | DB00562 Benzthiazide DB08846 Ellagic Acid DB00999 Hydrochlorothiazide DB00774 Hydroflumethiazide DB09460 Sodium carbonate DB05304 WX-G250 DB00909 Zonisamide |
Polymorphism and mutation databases
BioMutai | CA9 |
DMDMi | 83300925 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 37 | 1 PublicationAdd BLAST | 37 | |
ChainiPRO_0000004243 | 38 – 459 | Carbonic anhydrase 9Add BLAST | 422 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 115 | O-linked (GlcNAc...) threonine1 Publication | 1 | |
Disulfide bondi | 156 ↔ 336 | 1 Publication | ||
Disulfide bondi | 174 | Interchain1 Publication | ||
Glycosylationi | 346 | N-linked (GlcNAc...) asparagine2 Publications | 1 | |
Modified residuei | 449 | Phosphotyrosine1 Publication | 1 |
Post-translational modificationi
Asn-346 bears high-mannose type glycan structures.
Keywords - PTMi
Disulfide bond, Glycoprotein, PhosphoproteinProteomic databases
EPDi | Q16790 |
jPOSTi | Q16790 |
MaxQBi | Q16790 |
PaxDbi | Q16790 |
PeptideAtlasi | Q16790 |
PRIDEi | Q16790 |
ProteomicsDBi | 61071 |
PTM databases
iPTMneti | Q16790 |
PhosphoSitePlusi | Q16790 |
Expressioni
Tissue specificityi
Expressed primarily in carcinoma cells lines. Expression is restricted to very few normal tissues and the most abundant expression is found in the epithelial cells of gastric mucosa.
Inductioni
By hypoxia.1 Publication
Gene expression databases
Bgeei | ENSG00000107159 Expressed in 107 organ(s), highest expression level in body of stomach |
ExpressionAtlasi | Q16790 baseline and differential |
Genevisiblei | Q16790 HS |
Organism-specific databases
HPAi | CAB005100 CAB017107 HPA055207 |
Interactioni
Subunit structurei
Forms oligomers linked by disulfide bonds.3 Publications
Binary interactionsi
With | Entry | #Exp. | IntAct | Notes |
---|---|---|---|---|
GLRX3 | O76003 | 6 | EBI-12259996,EBI-374781 |
Protein-protein interaction databases
BioGridi | 107223, 33 interactors |
DIPi | DIP-48973N |
IntActi | Q16790, 2 interactors |
STRINGi | 9606.ENSP00000367608 |
Chemistry databases
BindingDBi | Q16790 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2HKF | X-ray | 2.01 | P | 83-91 | [»] | |
3IAI | X-ray | 2.20 | A/B/C/D | 137-391 | [»] | |
5DVX | X-ray | 1.60 | A/B | 140-399 | [»] | |
5FL4 | X-ray | 1.82 | A/B/C/D | 137-391 | [»] | |
5FL5 | X-ray | 2.05 | A/B/C/D | 137-391 | [»] | |
5FL6 | X-ray | 1.95 | A/B/C/D | 137-391 | [»] | |
6FE0 | X-ray | 1.91 | A/B/C/D | 137-391 | [»] | |
6FE1 | X-ray | 1.95 | A/B/C/D | 137-391 | [»] | |
6FE2 | X-ray | 1.87 | A/B/C/D | 137-391 | [»] | |
6G98 | X-ray | 2.47 | A/B/C/D | 135-391 | [»] | |
6G9U | X-ray | 1.75 | A/B/C/D | 137-391 | [»] | |
ProteinModelPortali | Q16790 | |||||
SMRi | Q16790 | |||||
ModBasei | Search... | |||||
MobiDBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q16790 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 139 – 390 | Alpha-carbonic anhydrasePROSITE-ProRule annotationAdd BLAST | 252 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 38 – 112 | Proteoglycan-like (PG)Add BLAST | 75 | |
Regioni | 113 – 414 | CatalyticAdd BLAST | 302 | |
Regioni | 332 – 333 | Substrate bindingBy similarity | 2 |
Sequence similaritiesi
Belongs to the alpha-carbonic anhydrase family.Curated
Keywords - Domaini
Signal, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG0382 Eukaryota COG3338 LUCA |
GeneTreei | ENSGT00940000161646 |
HOGENOMi | HOG000112637 |
HOVERGENi | HBG002837 |
InParanoidi | Q16790 |
KOi | K01672 |
OMAi | SRYFRYE |
OrthoDBi | 1377476at2759 |
PhylomeDBi | Q16790 |
TreeFami | TF316425 |
Family and domain databases
Gene3Di | 3.10.200.10, 1 hit |
InterProi | View protein in InterPro IPR018429 CA9 IPR001148 CA_dom IPR036398 CA_dom_sf IPR023561 Carbonic_anhydrase_a-class IPR018338 Carbonic_anhydrase_a-class_CS |
PANTHERi | PTHR18952 PTHR18952, 1 hit PTHR18952:SF18 PTHR18952:SF18, 1 hit |
Pfami | View protein in Pfam PF00194 Carb_anhydrase, 1 hit |
SMARTi | View protein in SMART SM01057 Carb_anhydrase, 1 hit |
SUPFAMi | SSF51069 SSF51069, 1 hit |
PROSITEi | View protein in PROSITE PS00162 ALPHA_CA_1, 1 hit PS51144 ALPHA_CA_2, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
Q16790-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAPLCPSPWL PLLIPAPAPG LTVQLLLSLL LLVPVHPQRL PRMQEDSPLG
60 70 80 90 100
GGSSGEDDPL GEEDLPSEED SPREEDPPGE EDLPGEEDLP GEEDLPEVKP
110 120 130 140 150
KSEEEGSLKL EDLPTVEAPG DPQEPQNNAH RDKEGDDQSH WRYGGDPPWP
160 170 180 190 200
RVSPACAGRF QSPVDIRPQL AAFCPALRPL ELLGFQLPPL PELRLRNNGH
210 220 230 240 250
SVQLTLPPGL EMALGPGREY RALQLHLHWG AAGRPGSEHT VEGHRFPAEI
260 270 280 290 300
HVVHLSTAFA RVDEALGRPG GLAVLAAFLE EGPEENSAYE QLLSRLEEIA
310 320 330 340 350
EEGSETQVPG LDISALLPSD FSRYFQYEGS LTTPPCAQGV IWTVFNQTVM
360 370 380 390 400
LSAKQLHTLS DTLWGPGDSR LQLNFRATQP LNGRVIEASF PAGVDSSPRA
410 420 430 440 450
AEPVQLNSCL AAGDILALVF GLLFAVTSVA FLVQMRRQHR RGTKGGVSYR
PAEVAETGA
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A087WYS4 | A0A087WYS4_HUMAN | Carbonic anhydrase 9 | CA9 | 356 | Annotation score: |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_010787 | 33 | V → M2 PublicationsCorresponds to variant dbSNP:rs2071676Ensembl. | 1 | |
Natural variantiVAR_020049 | 326 | Q → R. Corresponds to variant dbSNP:rs3829078Ensembl. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X66839 mRNA Translation: CAA47315.1 AJ010588 mRNA Translation: CAB82444.1 AL133410 Genomic DNA No translation available. AL357874 Genomic DNA No translation available. CH471071 Genomic DNA Translation: EAW58359.1 BC014950 mRNA Translation: AAH14950.1 |
CCDSi | CCDS6585.1 |
PIRi | I38013 |
RefSeqi | NP_001207.2, NM_001216.2 |
UniGenei | Hs.63287 |
Genome annotation databases
Ensembli | ENST00000378357; ENSP00000367608; ENSG00000107159 |
GeneIDi | 768 |
KEGGi | hsa:768 |
UCSCi | uc003zxo.5 human |
Keywords - Coding sequence diversityi
PolymorphismSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X66839 mRNA Translation: CAA47315.1 AJ010588 mRNA Translation: CAB82444.1 AL133410 Genomic DNA No translation available. AL357874 Genomic DNA No translation available. CH471071 Genomic DNA Translation: EAW58359.1 BC014950 mRNA Translation: AAH14950.1 |
CCDSi | CCDS6585.1 |
PIRi | I38013 |
RefSeqi | NP_001207.2, NM_001216.2 |
UniGenei | Hs.63287 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2HKF | X-ray | 2.01 | P | 83-91 | [»] | |
3IAI | X-ray | 2.20 | A/B/C/D | 137-391 | [»] | |
5DVX | X-ray | 1.60 | A/B | 140-399 | [»] | |
5FL4 | X-ray | 1.82 | A/B/C/D | 137-391 | [»] | |
5FL5 | X-ray | 2.05 | A/B/C/D | 137-391 | [»] | |
5FL6 | X-ray | 1.95 | A/B/C/D | 137-391 | [»] | |
6FE0 | X-ray | 1.91 | A/B/C/D | 137-391 | [»] | |
6FE1 | X-ray | 1.95 | A/B/C/D | 137-391 | [»] | |
6FE2 | X-ray | 1.87 | A/B/C/D | 137-391 | [»] | |
6G98 | X-ray | 2.47 | A/B/C/D | 135-391 | [»] | |
6G9U | X-ray | 1.75 | A/B/C/D | 137-391 | [»] | |
ProteinModelPortali | Q16790 | |||||
SMRi | Q16790 | |||||
ModBasei | Search... | |||||
MobiDBi | Search... |
Protein-protein interaction databases
BioGridi | 107223, 33 interactors |
DIPi | DIP-48973N |
IntActi | Q16790, 2 interactors |
STRINGi | 9606.ENSP00000367608 |
Chemistry databases
BindingDBi | Q16790 |
ChEMBLi | CHEMBL3594 |
DrugBanki | DB00562 Benzthiazide DB08846 Ellagic Acid DB00999 Hydrochlorothiazide DB00774 Hydroflumethiazide DB09460 Sodium carbonate DB05304 WX-G250 DB00909 Zonisamide |
PTM databases
iPTMneti | Q16790 |
PhosphoSitePlusi | Q16790 |
Polymorphism and mutation databases
BioMutai | CA9 |
DMDMi | 83300925 |
Proteomic databases
EPDi | Q16790 |
jPOSTi | Q16790 |
MaxQBi | Q16790 |
PaxDbi | Q16790 |
PeptideAtlasi | Q16790 |
PRIDEi | Q16790 |
ProteomicsDBi | 61071 |
Protocols and materials databases
DNASUi | 768 |
Structural Biology Knowledgebase | Search... |
Genome annotation databases
Ensembli | ENST00000378357; ENSP00000367608; ENSG00000107159 |
GeneIDi | 768 |
KEGGi | hsa:768 |
UCSCi | uc003zxo.5 human |
Organism-specific databases
CTDi | 768 |
DisGeNETi | 768 |
EuPathDBi | HostDB:ENSG00000107159.12 |
GeneCardsi | CA9 |
H-InvDBi | HIX0008019 |
HGNCi | HGNC:1383 CA9 |
HPAi | CAB005100 CAB017107 HPA055207 |
MIMi | 603179 gene |
neXtProti | NX_Q16790 |
OpenTargetsi | ENSG00000107159 |
PharmGKBi | PA25998 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG0382 Eukaryota COG3338 LUCA |
GeneTreei | ENSGT00940000161646 |
HOGENOMi | HOG000112637 |
HOVERGENi | HBG002837 |
InParanoidi | Q16790 |
KOi | K01672 |
OMAi | SRYFRYE |
OrthoDBi | 1377476at2759 |
PhylomeDBi | Q16790 |
TreeFami | TF316425 |
Enzyme and pathway databases
BRENDAi | 4.2.1.1 2681 |
Reactomei | R-HSA-1234158 Regulation of gene expression by Hypoxia-inducible Factor R-HSA-1475029 Reversible hydration of carbon dioxide |
SIGNORi | Q16790 |
Miscellaneous databases
ChiTaRSi | CA9 human |
EvolutionaryTracei | Q16790 |
GeneWikii | Carbonic_anhydrase_9 |
GenomeRNAii | 768 |
PROi | PR:Q16790 |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000107159 Expressed in 107 organ(s), highest expression level in body of stomach |
ExpressionAtlasi | Q16790 baseline and differential |
Genevisiblei | Q16790 HS |
Family and domain databases
Gene3Di | 3.10.200.10, 1 hit |
InterProi | View protein in InterPro IPR018429 CA9 IPR001148 CA_dom IPR036398 CA_dom_sf IPR023561 Carbonic_anhydrase_a-class IPR018338 Carbonic_anhydrase_a-class_CS |
PANTHERi | PTHR18952 PTHR18952, 1 hit PTHR18952:SF18 PTHR18952:SF18, 1 hit |
Pfami | View protein in Pfam PF00194 Carb_anhydrase, 1 hit |
SMARTi | View protein in SMART SM01057 Carb_anhydrase, 1 hit |
SUPFAMi | SSF51069 SSF51069, 1 hit |
PROSITEi | View protein in PROSITE PS00162 ALPHA_CA_1, 1 hit PS51144 ALPHA_CA_2, 1 hit |
ProtoNeti | Search... |
Entry informationi
Entry namei | CAH9_HUMAN | |
Accessioni | Q16790Primary (citable) accession number: Q16790 Secondary accession number(s): Q5T4R1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 15, 1999 |
Last sequence update: | December 6, 2005 | |
Last modified: | February 13, 2019 | |
This is version 179 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Complete proteome, Direct protein sequencing, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - Human chromosome 9
Human chromosome 9: entries, gene names and cross-references to MIM - Human entries with polymorphisms or disease mutations
List of human entries with polymorphisms or disease mutations - Human polymorphisms and disease mutations
Index of human polymorphisms and disease mutations