Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Guanylate kinase

Gene

GUK1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Essential for recycling GMP and indirectly, cGMP.

Miscellaneous

In addition to phosphorylating GMP, antiviral prodrugs such as acyclovir, ganciclovir, and carbovir and anticancer prodrugs such as the thiopurines are dependent on GMPK for their activation.

Catalytic activityi

ATP + GMP = ADP + GDP.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei44By similarity1
Active sitei137By similarity1
Active sitei148By similarity1
Binding sitei171ATPBy similarity1
Binding sitei172ATP; via carbonyl oxygenBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi11 – 18ATPPROSITE-ProRule annotation8

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • guanylate kinase activity Source: UniProtKB

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMetaCyc:HS07104-MONOMER
BRENDAi2.7.4.8 2681
ReactomeiR-HSA-2161541 Abacavir metabolism
R-HSA-499943 Interconversion of nucleotide di- and triphosphates
SABIO-RKiQ16774

Names & Taxonomyi

Protein namesi
Recommended name:
Guanylate kinase (EC:2.7.4.8)
Alternative name(s):
GMP kinase
Gene namesi
Name:GUK1
Synonyms:GMK
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

EuPathDBiHostDB:ENSG00000143774.16
HGNCiHGNC:4693 GUK1
MIMi139270 gene
neXtProtiNX_Q16774

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Pathology & Biotechi

Organism-specific databases

DisGeNETi2987
OpenTargetsiENSG00000143774
PharmGKBiPA29072

Chemistry databases

ChEMBLiCHEMBL4989
DrugBankiDB01972 Guanosine-5'-Monophosphate

Polymorphism and mutation databases

BioMutaiGUK1
DMDMi2497498

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00001706512 – 197Guanylate kinaseAdd BLAST196

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

EPDiQ16774
PaxDbiQ16774
PeptideAtlasiQ16774
PRIDEiQ16774
ProteomicsDBi61062
61063 [Q16774-2]

PTM databases

iPTMnetiQ16774
PhosphoSitePlusiQ16774

Expressioni

Gene expression databases

BgeeiENSG00000143774 Expressed in 229 organ(s), highest expression level in dorsolateral prefrontal cortex
CleanExiHS_GUK1
ExpressionAtlasiQ16774 baseline and differential
GenevisibleiQ16774 HS

Organism-specific databases

HPAiHPA048587

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

BioGridi109242, 14 interactors
IntActiQ16774, 5 interactors
STRINGi9606.ENSP00000355689

Structurei

3D structure databases

ProteinModelPortaliQ16774
SMRiQ16774
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini4 – 186Guanylate kinase-likePROSITE-ProRule annotationAdd BLAST183

Sequence similaritiesi

Belongs to the guanylate kinase family.Curated

Phylogenomic databases

eggNOGiKOG0707 Eukaryota
COG0194 LUCA
GeneTreeiENSGT00760000118866
HOGENOMiHOG000037640
HOVERGENiHBG003344
InParanoidiQ16774
KOiK00942
PhylomeDBiQ16774
TreeFamiTF314473

Family and domain databases

InterProiView protein in InterPro
IPR008145 GK/Ca_channel_bsu
IPR008144 Guanylate_kin-like_dom
IPR017665 Guanylate_kinase
IPR020590 Guanylate_kinase_CS
IPR027417 P-loop_NTPase
PfamiView protein in Pfam
PF00625 Guanylate_kin, 1 hit
SMARTiView protein in SMART
SM00072 GuKc, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR03263 guanyl_kin, 1 hit
PROSITEiView protein in PROSITE
PS00856 GUANYLATE_KINASE_1, 1 hit
PS50052 GUANYLATE_KINASE_2, 1 hit

Sequences (3+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 3 described isoforms and 6 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q16774-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MSGPRPVVLS GPSGAGKSTL LKRLLQEHSG IFGFSVSHTT RNPRPGEENG
60 70 80 90 100
KDYYFVTREV MQRDIAAGDF IEHAEFSGNL YGTSKVAVQA VQAMNRICVL
110 120 130 140 150
DVDLQGVRNI KATDLRPIYI SVQPPSLHVL EQRLRQRNTE TEESLVKRLA
160 170 180 190
AAQADMESSK EPGLFDVVII NDSLDQAYAE LKEALSEEIK KAQRTGA
Length:197
Mass (Da):21,726
Last modified:January 23, 2007 - v2
Checksum:iC49573212A0DA825
GO
Isoform 2 (identifier: Q16774-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MLRRPLAGLAAAALGRAPPDGM

Show »
Length:218
Mass (Da):23,782
Checksum:i6972858284006BB2
GO
Isoform 3 (identifier: Q16774-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MLRRPLAGLAAAALGRAPPDGM
     159-197: SKEPGLFDVV...EIKKAQRTGA → RNQESSKDRR...CCPCGWNILG

Note: Gene prediction based on EST data.
Show »
Length:241
Mass (Da):26,596
Checksum:i418604D9B391F864
GO

Computationally mapped potential isoform sequencesi

There are 6 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
B1ANH0B1ANH0_HUMAN
Guanylate kinase
GUK1
217Annotation score:
B1ANG9B1ANG9_HUMAN
Guanylate kinase
GUK1
235Annotation score:
B1ANH5B1ANH5_HUMAN
Guanylate kinase
GUK1
199Annotation score:
B1ANH2B1ANH2_HUMAN
Guanylate kinase
GUK1
253Annotation score:
B1ANH6B1ANH6_HUMAN
Guanylate kinase
GUK1
242Annotation score:
B1ANH3B1ANH3_HUMAN
Guanylate kinase
GUK1
272Annotation score:

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0437781M → MLRRPLAGLAAAALGRAPPD GM in isoform 2 and isoform 3. 1 Publication1
Alternative sequenceiVSP_047372159 – 197SKEPG…QRTGA → RNQESSKDRRLRLAVCSRHP GPIQDQGSSIEPPPWQAIRQ LCALGQHVEWRRCCPCGWNI LG in isoform 3. CuratedAdd BLAST39

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L76200 mRNA Translation: AAC37598.1
U66895 mRNA Translation: AAC50659.1
AK303845 mRNA Translation: BAG64788.1
AL359510 Genomic DNA No translation available.
CH471098 Genomic DNA Translation: EAW69856.1
BC006249 mRNA Translation: AAH06249.1
BC009914 mRNA Translation: AAH09914.1
CCDSiCCDS1568.1 [Q16774-1]
CCDS53481.1 [Q16774-2]
CCDS55689.1 [Q16774-3]
PIRiS68864
RefSeqiNP_000849.1, NM_000858.5 [Q16774-1]
NP_001152862.1, NM_001159390.1 [Q16774-2]
NP_001152863.1, NM_001159391.1 [Q16774-1]
NP_001229768.1, NM_001242839.1 [Q16774-1]
NP_001229769.1, NM_001242840.1 [Q16774-3]
UniGeneiHs.376933

Genome annotation databases

EnsembliENST00000312726; ENSP00000317659; ENSG00000143774 [Q16774-1]
ENST00000366716; ENSP00000355677; ENSG00000143774 [Q16774-1]
ENST00000366718; ENSP00000355679; ENSG00000143774 [Q16774-1]
ENST00000366726; ENSP00000355687; ENSG00000143774 [Q16774-1]
ENST00000366728; ENSP00000355689; ENSG00000143774 [Q16774-3]
ENST00000366730; ENSP00000355691; ENSG00000143774 [Q16774-1]
ENST00000391865; ENSP00000375738; ENSG00000143774 [Q16774-2]
GeneIDi2987
KEGGihsa:2987
UCSCiuc001hsi.4 human [Q16774-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L76200 mRNA Translation: AAC37598.1
U66895 mRNA Translation: AAC50659.1
AK303845 mRNA Translation: BAG64788.1
AL359510 Genomic DNA No translation available.
CH471098 Genomic DNA Translation: EAW69856.1
BC006249 mRNA Translation: AAH06249.1
BC009914 mRNA Translation: AAH09914.1
CCDSiCCDS1568.1 [Q16774-1]
CCDS53481.1 [Q16774-2]
CCDS55689.1 [Q16774-3]
PIRiS68864
RefSeqiNP_000849.1, NM_000858.5 [Q16774-1]
NP_001152862.1, NM_001159390.1 [Q16774-2]
NP_001152863.1, NM_001159391.1 [Q16774-1]
NP_001229768.1, NM_001242839.1 [Q16774-1]
NP_001229769.1, NM_001242840.1 [Q16774-3]
UniGeneiHs.376933

3D structure databases

ProteinModelPortaliQ16774
SMRiQ16774
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi109242, 14 interactors
IntActiQ16774, 5 interactors
STRINGi9606.ENSP00000355689

Chemistry databases

ChEMBLiCHEMBL4989
DrugBankiDB01972 Guanosine-5'-Monophosphate

PTM databases

iPTMnetiQ16774
PhosphoSitePlusiQ16774

Polymorphism and mutation databases

BioMutaiGUK1
DMDMi2497498

Proteomic databases

EPDiQ16774
PaxDbiQ16774
PeptideAtlasiQ16774
PRIDEiQ16774
ProteomicsDBi61062
61063 [Q16774-2]

Protocols and materials databases

DNASUi2987
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000312726; ENSP00000317659; ENSG00000143774 [Q16774-1]
ENST00000366716; ENSP00000355677; ENSG00000143774 [Q16774-1]
ENST00000366718; ENSP00000355679; ENSG00000143774 [Q16774-1]
ENST00000366726; ENSP00000355687; ENSG00000143774 [Q16774-1]
ENST00000366728; ENSP00000355689; ENSG00000143774 [Q16774-3]
ENST00000366730; ENSP00000355691; ENSG00000143774 [Q16774-1]
ENST00000391865; ENSP00000375738; ENSG00000143774 [Q16774-2]
GeneIDi2987
KEGGihsa:2987
UCSCiuc001hsi.4 human [Q16774-1]

Organism-specific databases

CTDi2987
DisGeNETi2987
EuPathDBiHostDB:ENSG00000143774.16
GeneCardsiGUK1
HGNCiHGNC:4693 GUK1
HPAiHPA048587
MIMi139270 gene
neXtProtiNX_Q16774
OpenTargetsiENSG00000143774
PharmGKBiPA29072
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0707 Eukaryota
COG0194 LUCA
GeneTreeiENSGT00760000118866
HOGENOMiHOG000037640
HOVERGENiHBG003344
InParanoidiQ16774
KOiK00942
PhylomeDBiQ16774
TreeFamiTF314473

Enzyme and pathway databases

BioCyciMetaCyc:HS07104-MONOMER
BRENDAi2.7.4.8 2681
ReactomeiR-HSA-2161541 Abacavir metabolism
R-HSA-499943 Interconversion of nucleotide di- and triphosphates
SABIO-RKiQ16774

Miscellaneous databases

ChiTaRSiGUK1 human
GeneWikiiGUK1
GenomeRNAii2987
PROiPR:Q16774
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000143774 Expressed in 229 organ(s), highest expression level in dorsolateral prefrontal cortex
CleanExiHS_GUK1
ExpressionAtlasiQ16774 baseline and differential
GenevisibleiQ16774 HS

Family and domain databases

InterProiView protein in InterPro
IPR008145 GK/Ca_channel_bsu
IPR008144 Guanylate_kin-like_dom
IPR017665 Guanylate_kinase
IPR020590 Guanylate_kinase_CS
IPR027417 P-loop_NTPase
PfamiView protein in Pfam
PF00625 Guanylate_kin, 1 hit
SMARTiView protein in SMART
SM00072 GuKc, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR03263 guanyl_kin, 1 hit
PROSITEiView protein in PROSITE
PS00856 GUANYLATE_KINASE_1, 1 hit
PS50052 GUANYLATE_KINASE_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiKGUA_HUMAN
AccessioniPrimary (citable) accession number: Q16774
Secondary accession number(s): B1ANH1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: January 23, 2007
Last modified: October 10, 2018
This is version 159 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  3. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again