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UniProtKB - Q16763 (UBE2S_HUMAN)

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Protein

Ubiquitin-conjugating enzyme E2 S

Gene

UBE2S

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins (PubMed:22496338). Catalyzes 'Lys-11'-linked polyubiquitination. Acts as an essential factor of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated ubiquitin ligase that controls progression through mitosis. Acts by specifically elongating 'Lys-11'-linked polyubiquitin chains initiated by the E2 enzyme UBE2C/UBCH10 on APC/C substrates, enhancing the degradation of APC/C substrates by the proteasome and promoting mitotic exit (PubMed:19820702, PubMed:19822757, PubMed:27259151). Also acts by elongating ubiquitin chains initiated by the E2 enzyme UBE2D1/UBCH5 in vitro; it is however unclear whether UBE2D1/UBCH5 acts as an E2 enzyme for the APC/C in vivo. Also involved in ubiquitination and subsequent degradation of VHL, resulting in an accumulation of HIF1A (PubMed:16819549). In vitro able to promote polyubiquitination using all 7 ubiquitin Lys residues, except 'Lys-48'-linked polyubiquitination (PubMed:20061386, PubMed:20622874).7 Publications

Catalytic activityi

S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine.PROSITE-ProRule annotation3 Publications

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.PROSITE-ProRule annotation2 Publications
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei95Glycyl thioester intermediate1

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • ubiquitin conjugating enzyme activity Source: MGI
  • ubiquitin protein ligase activity Source: GO_Central
  • ubiquitin protein ligase binding Source: GO_Central
  • ubiquitin-protein transferase activity Source: UniProtKB
View the complete GO annotation on QuickGO ...

GO - Biological processi

  • anaphase-promoting complex-dependent catabolic process Source: UniProtKB
  • cell division Source: UniProtKB-KW
  • cellular protein modification process Source: ProtInc
  • exit from mitosis Source: UniProtKB
  • free ubiquitin chain polymerization Source: UniProtKB
  • positive regulation of ubiquitin protein ligase activity Source: UniProtKB
  • protein K11-linked ubiquitination Source: UniProtKB
  • protein K27-linked ubiquitination Source: UniProtKB
  • protein K29-linked ubiquitination Source: UniProtKB
  • protein K63-linked ubiquitination Source: UniProtKB
  • protein K6-linked ubiquitination Source: UniProtKB
  • protein ubiquitination Source: Reactome
View the complete GO annotation on QuickGO ...

Keywordsi

Molecular functionTransferase
Biological processCell cycle, Cell division, Ubl conjugation pathway
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.3.2.B6 2681
ReactomeiR-HSA-8866652 Synthesis of active ubiquitin: roles of E1 and E2 enzymes
R-HSA-983168 Antigen processing: Ubiquitination & Proteasome degradation
SignaLinkiQ16763
UniPathwayiUPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-conjugating enzyme E2 S (EC:2.3.2.233 Publications)
Alternative name(s):
E2 ubiquitin-conjugating enzyme S
E2-EPF
Ubiquitin carrier protein S
Ubiquitin-conjugating enzyme E2-24 kDa
Ubiquitin-conjugating enzyme E2-EPF5
Ubiquitin-protein ligase S
Gene namesi
Name:UBE2S
Synonyms:E2EPF
ORF Names:OK/SW-cl.73
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo
Proteomesi
  • UP000005640 Componenti: Chromosome 19

Organism-specific databases

EuPathDBiHostDB:ENSG00000108106.13
HGNCiHGNC:17895 UBE2S
MIMi610309 gene
neXtProtiNX_Q16763

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi95C → S: Loss of function. 2 Publications1
Mutagenesisi117K → A: Reduced ubiquitination activity. 1 Publication1
Mutagenesisi222L → A: Impairs polyubiquitination in the presence of APC/C complex, decreasing affinity for substrate. 1 Publication1

Organism-specific databases

DisGeNETi27338
OpenTargetsiENSG00000108106
PharmGKBiPA134904441

Polymorphism and mutation databases

BioMutaiUBE2S
DMDMi23829978

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000825071 – 222Ubiquitin-conjugating enzyme E2 SAdd BLAST222

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineCombined sources1
Modified residuei173PhosphoserineCombined sources1

Post-translational modificationi

Autoubiquitinated by the APC/C complex during G1, leading to its degradation by the proteasome.1 Publication

Keywords - PTMi

Acetylation, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ16763
MaxQBiQ16763
PaxDbiQ16763
PeptideAtlasiQ16763
PRIDEiQ16763
ProteomicsDBi61055

PTM databases

iPTMnetiQ16763
PhosphoSitePlusiQ16763

Expressioni

Gene expression databases

BgeeiENSG00000108106
CleanExiHS_UBE2S
ExpressionAtlasiQ16763 baseline and differential
GenevisibleiQ16763 HS

Organism-specific databases

HPAiCAB015228
HPA057150

Interactioni

Subunit structurei

Component of the APC/C complex, composed of at least 14 distinct subunits that assemble into a complex of at least 19 chains with a combined molecular mass of around 1.2 MDa. Within this complex, directly interacts with ANAPC2 and ANAPC4 (PubMed:27259151). Interacts with CDC20, FZR1/CDH1 and VHL (PubMed:16819549, PubMed:19822757).3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
ANAPC2Q9UJX64EBI-2339823,EBI-396211

GO - Molecular functioni

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

BioGridi118150, 86 interactors
DIPiDIP-52784N
IntActiQ16763, 15 interactors
MINTiQ16763
STRINGi9606.ENSP00000264552

Structurei

Secondary structure

1222
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi10 – 25Combined sources16
Beta strandi31 – 35Combined sources5
Beta strandi42 – 48Combined sources7
Turni54 – 57Combined sources4
Beta strandi59 – 65Combined sources7
Turni68 – 72Combined sources5
Beta strandi76 – 81Combined sources6
Beta strandi92 – 94Combined sources3
Helixi96 – 99Combined sources4
Turni100 – 102Combined sources3
Helixi109 – 121Combined sources13
Helixi125 – 127Combined sources3
Helixi131 – 139Combined sources9
Helixi141 – 155Combined sources15

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1ZDNX-ray1.93A/B1-156[»]
5BNBX-ray2.49A/B/C/D1-156[»]
5L9Telectron microscopy6.40T1-222[»]
ProteinModelPortaliQ16763
SMRiQ16763
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ16763

Family & Domainsi

Sequence similaritiesi

Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0423 Eukaryota
COG5078 LUCA
GeneTreeiENSGT00910000143990
HOGENOMiHOG000233455
HOVERGENiHBG063308
InParanoidiQ16763
KOiK10583
OrthoDBiEOG091G0USF
PhylomeDBiQ16763
TreeFamiTF101120

Family and domain databases

CDDicd00195 UBCc, 1 hit
Gene3Di3.10.110.10, 1 hit
InterProiView protein in InterPro
IPR000608 UBQ-conjugat_E2
IPR023313 UBQ-conjugating_AS
IPR016135 UBQ-conjugating_enzyme/RWD
PfamiView protein in Pfam
PF00179 UQ_con, 1 hit
SUPFAMiSSF54495 SSF54495, 1 hit
PROSITEiView protein in PROSITE
PS00183 UBIQUITIN_CONJUGAT_1, 1 hit
PS50127 UBIQUITIN_CONJUGAT_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q16763-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNSNVENLPP HIIRLVYKEV TTLTADPPDG IKVFPNEEDL TDLQVTIEGP 
        60         70         80         90        100
EGTPYAGGLF RMKLLLGKDF PASPPKGYFL TKIFHPNVGA NGEICVNVLK 
       110        120        130        140        150
RDWTAELGIR HVLLTIKCLL IHPNPESALN EEAGRLLLEN YEEYAARARL 
       160        170        180        190        200
LTEIHGGAGG PSGRAEAGRA LASGTEASST DPGAPGGPGG AEGPMAKKHA 
       210        220 
GERDKKLAAK KKTDKKRALR RL
Length:222
Mass (Da):23,845
Last modified:October 10, 2002 - v2
Checksum:i2842DC3DCD2AFCB5
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti216K → KRAL in AAA58446 (PubMed:1379239).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M91670 mRNA Translation: AAA58446.1
AB062397 mRNA Translation: BAB93484.1
AC020922 Genomic DNA No translation available.
BC004236 mRNA Translation: AAH04236.1
BC007554 mRNA Translation: AAH07554.1
BC065364 mRNA Translation: AAH65364.1
CCDSiCCDS33114.1
RefSeqiNP_055316.2, NM_014501.2
UniGeneiHs.396393

Genome annotation databases

EnsembliENST00000264552; ENSP00000264552; ENSG00000108106
GeneIDi27338
KEGGihsa:27338
UCSCiuc002qkx.2 human

Similar proteinsi

Entry informationi

Entry nameiUBE2S_HUMAN
AccessioniPrimary (citable) accession number: Q16763
Secondary accession number(s): Q9BTC1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: October 10, 2002
Last modified: June 20, 2018
This is version 175 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  4. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  5. SIMILARITY comments
    Index of protein domains and families

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