UniProtKB - Q16763 (UBE2S_HUMAN)
Ubiquitin-conjugating enzyme E2 S
UBE2S
Functioni
Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins (PubMed:22496338).
Catalyzes 'Lys-11'-linked polyubiquitination. Acts as an essential factor of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated ubiquitin ligase that controls progression through mitosis. Acts by specifically elongating 'Lys-11'-linked polyubiquitin chains initiated by the E2 enzyme UBE2C/UBCH10 on APC/C substrates, enhancing the degradation of APC/C substrates by the proteasome and promoting mitotic exit (PubMed:19820702, PubMed:19822757, PubMed:27259151).
Also acts by elongating ubiquitin chains initiated by the E2 enzyme UBE2D1/UBCH5 in vitro; it is however unclear whether UBE2D1/UBCH5 acts as an E2 enzyme for the APC/C in vivo. Also involved in ubiquitination and subsequent degradation of VHL, resulting in an accumulation of HIF1A (PubMed:16819549).
In vitro able to promote polyubiquitination using all 7 ubiquitin Lys residues, except 'Lys-48'-linked polyubiquitination (PubMed:20061386, PubMed:20622874).
7 PublicationsCatalytic activityi
- S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine.PROSITE-ProRule annotation3 Publications EC:2.3.2.23
: protein ubiquitination Pathwayi
This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.PROSITE-ProRule annotation2 PublicationsView all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 95 | Glycyl thioester intermediatePROSITE-ProRule annotation | 1 |
GO - Molecular functioni
- anaphase-promoting complex binding Source: UniProtKB
- ATP binding Source: UniProtKB-KW
- ubiquitin conjugating enzyme activity Source: MGI
- ubiquitin-protein transferase activity Source: UniProtKB
GO - Biological processi
- anaphase-promoting complex-dependent catabolic process Source: UniProtKB
- cell division Source: UniProtKB-KW
- cellular protein modification process Source: ProtInc
- exit from mitosis Source: UniProtKB
- free ubiquitin chain polymerization Source: UniProtKB
- positive regulation of ubiquitin protein ligase activity Source: UniProtKB
- protein K11-linked ubiquitination Source: UniProtKB
- protein K27-linked ubiquitination Source: UniProtKB
- protein K29-linked ubiquitination Source: UniProtKB
- protein K63-linked ubiquitination Source: UniProtKB
- protein K6-linked ubiquitination Source: UniProtKB
- protein polyubiquitination Source: GO_Central
- ubiquitin-dependent protein catabolic process Source: GO_Central
Keywordsi
Molecular function | Transferase |
Biological process | Cell cycle, Cell division, Ubl conjugation pathway |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BRENDAi | 2.3.2.24, 2681 |
PathwayCommonsi | Q16763 |
Reactomei | R-HSA-141430, Inactivation of APC/C via direct inhibition of the APC/C complex R-HSA-174048, APC/C:Cdc20 mediated degradation of Cyclin B R-HSA-174084, Autodegradation of Cdh1 by Cdh1:APC/C R-HSA-174154, APC/C:Cdc20 mediated degradation of Securin R-HSA-174178, APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1 R-HSA-174184, Cdc20:Phospho-APC/C mediated degradation of Cyclin A R-HSA-176407, Conversion from APC/C:Cdc20 to APC/C:Cdh1 in late anaphase R-HSA-176408, Regulation of APC/C activators between G1/S and early anaphase R-HSA-176409, APC/C:Cdc20 mediated degradation of mitotic proteins R-HSA-176412, Phosphorylation of the APC/C R-HSA-179409, APC-Cdc20 mediated degradation of Nek2A R-HSA-2467813, Separation of Sister Chromatids R-HSA-2559582, Senescence-Associated Secretory Phenotype (SASP) R-HSA-69017, CDK-mediated phosphorylation and removal of Cdc6 R-HSA-8853884, Transcriptional Regulation by VENTX R-HSA-8866652, Synthesis of active ubiquitin: roles of E1 and E2 enzymes R-HSA-9687136, Aberrant regulation of mitotic exit in cancer due to RB1 defects R-HSA-983168, Antigen processing: Ubiquitination & Proteasome degradation |
SignaLinki | Q16763 |
SIGNORi | Q16763 |
UniPathwayi | UPA00143 |
Names & Taxonomyi
Protein namesi | Recommended name: Ubiquitin-conjugating enzyme E2 S (EC:2.3.2.233 Publications)Alternative name(s): E2 ubiquitin-conjugating enzyme S E2-EPF Ubiquitin carrier protein S Ubiquitin-conjugating enzyme E2-24 kDa Ubiquitin-conjugating enzyme E2-EPF5 Ubiquitin-protein ligase S |
Gene namesi | Name:UBE2S Synonyms:E2EPF ORF Names:OK/SW-cl.73 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:17895, UBE2S |
MIMi | 610309, gene |
neXtProti | NX_Q16763 |
VEuPathDBi | HostDB:ENSG00000108106 |
Subcellular locationi
Cytosol
- cytosol Source: Reactome
Nucleus
- anaphase-promoting complex Source: UniProtKB
- nucleoplasm Source: Reactome
- nucleus Source: GO_Central
Pathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 95 | C → S: Loss of function. 2 Publications | 1 | |
Mutagenesisi | 117 | K → A: Reduced ubiquitination activity. 1 Publication | 1 | |
Mutagenesisi | 222 | L → A: Impairs polyubiquitination in the presence of APC/C complex, decreasing affinity for substrate. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 27338 |
OpenTargetsi | ENSG00000108106 |
PharmGKBi | PA134904441 |
Miscellaneous databases
Pharosi | Q16763, Tbio |
Genetic variation databases
BioMutai | UBE2S |
DMDMi | 23829978 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000082507 | 1 – 222 | Ubiquitin-conjugating enzyme E2 SAdd BLAST | 222 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 1 | N-acetylmethionineCombined sources | 1 | |
Modified residuei | 173 | PhosphoserineCombined sources | 1 |
Post-translational modificationi
Keywords - PTMi
Acetylation, Phosphoprotein, Ubl conjugationProteomic databases
EPDi | Q16763 |
jPOSTi | Q16763 |
MassIVEi | Q16763 |
MaxQBi | Q16763 |
PaxDbi | Q16763 |
PeptideAtlasi | Q16763 |
PRIDEi | Q16763 |
ProteomicsDBi | 61055 |
PTM databases
iPTMneti | Q16763 |
MetOSitei | Q16763 |
PhosphoSitePlusi | Q16763 |
SwissPalmi | Q16763 |
Expressioni
Gene expression databases
Bgeei | ENSG00000108106, Expressed in testis and 115 other tissues |
ExpressionAtlasi | Q16763, baseline and differential |
Genevisiblei | Q16763, HS |
Organism-specific databases
HPAi | ENSG00000108106, Tissue enhanced (bone marrow, testis) |
Interactioni
Subunit structurei
Component of the APC/C complex, composed of at least 14 distinct subunits that assemble into a complex of at least 19 chains with a combined molecular mass of around 1.2 MDa. Within this complex, directly interacts with ANAPC2 and ANAPC4 (PubMed:27259151).
Interacts with CDC20, FZR1/CDH1 and VHL (PubMed:16819549, PubMed:19822757).
3 PublicationsBinary interactionsi
Q16763
With | #Exp. | IntAct |
---|---|---|
ANAPC2 [Q9UJX6] | 4 | EBI-2339823,EBI-396211 |
GNB2 [P62879] | 3 | EBI-2339823,EBI-356942 |
VHL - isoform 2 [P40337-2] | 3 | EBI-2339823,EBI-12157263 |
Protein-protein interaction databases
BioGRIDi | 118150, 125 interactors |
DIPi | DIP-52784N |
IntActi | Q16763, 23 interactors |
MINTi | Q16763 |
STRINGi | 9606.ENSP00000264552 |
Miscellaneous databases
RNActi | Q16763, protein |
Structurei
Secondary structure
3D structure databases
AlphaFoldDBi | Q16763 |
BMRBi | Q16763 |
SMRi | Q16763 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q16763 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 11 – 157 | UBC corePROSITE-ProRule annotationAdd BLAST | 147 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 156 – 222 | DisorderedSequence analysisAdd BLAST | 67 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0423, Eukaryota |
GeneTreei | ENSGT00940000157149 |
InParanoidi | Q16763 |
OMAi | QPAKCGA |
OrthoDBi | 1412570at2759 |
PhylomeDBi | Q16763 |
TreeFami | TF101120 |
Family and domain databases
CDDi | cd00195, UBCc, 1 hit |
Gene3Di | 3.10.110.10, 1 hit |
InterProi | View protein in InterPro IPR000608, UBQ-conjugat_E2 IPR023313, UBQ-conjugating_AS IPR016135, UBQ-conjugating_enzyme/RWD |
Pfami | View protein in Pfam PF00179, UQ_con, 1 hit |
SUPFAMi | SSF54495, SSF54495, 1 hit |
PROSITEi | View protein in PROSITE PS00183, UBC_1, 1 hit PS50127, UBC_2, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
10 20 30 40 50
MNSNVENLPP HIIRLVYKEV TTLTADPPDG IKVFPNEEDL TDLQVTIEGP
60 70 80 90 100
EGTPYAGGLF RMKLLLGKDF PASPPKGYFL TKIFHPNVGA NGEICVNVLK
110 120 130 140 150
RDWTAELGIR HVLLTIKCLL IHPNPESALN EEAGRLLLEN YEEYAARARL
160 170 180 190 200
LTEIHGGAGG PSGRAEAGRA LASGTEASST DPGAPGGPGG AEGPMAKKHA
210 220
GERDKKLAAK KKTDKKRALR RL
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketK7EPJ1 | K7EPJ1_HUMAN | Ubiquitin-conjugating enzyme E2 S | UBE2S | 204 | Annotation score: | ||
K7EM75 | K7EM75_HUMAN | Ubiquitin-conjugating enzyme E2 S | UBE2S | 79 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 216 | K → KRAL in AAA58446 (PubMed:1379239).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M91670 mRNA Translation: AAA58446.1 AB062397 mRNA Translation: BAB93484.1 AC020922 Genomic DNA No translation available. BC004236 mRNA Translation: AAH04236.1 BC007554 mRNA Translation: AAH07554.1 BC065364 mRNA Translation: AAH65364.1 |
CCDSi | CCDS33114.1 |
RefSeqi | NP_055316.2, NM_014501.2 |
Genome annotation databases
Ensembli | ENST00000264552.14; ENSP00000264552.8; ENSG00000108106.14 |
GeneIDi | 27338 |
KEGGi | hsa:27338 |
MANE-Selecti | ENST00000264552.14; ENSP00000264552.8; NM_014501.3; NP_055316.2 |
UCSCi | uc002qkx.2, human |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M91670 mRNA Translation: AAA58446.1 AB062397 mRNA Translation: BAB93484.1 AC020922 Genomic DNA No translation available. BC004236 mRNA Translation: AAH04236.1 BC007554 mRNA Translation: AAH07554.1 BC065364 mRNA Translation: AAH65364.1 |
CCDSi | CCDS33114.1 |
RefSeqi | NP_055316.2, NM_014501.2 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1ZDN | X-ray | 1.93 | A/B | 1-156 | [»] | |
5BNB | X-ray | 2.49 | A/B/C/D | 1-156 | [»] | |
5L9T | electron microscopy | 6.40 | T | 1-222 | [»] | |
6QH3 | X-ray | 2.90 | A/B | 1-156 | [»] | |
6QHK | X-ray | 1.96 | A/B | 1-156 | [»] | |
6S96 | X-ray | 2.18 | A/B | 1-156 | [»] | |
6S98 | X-ray | 1.55 | A/B | 1-156 | [»] | |
7AHF | X-ray | 2.15 | A/B | 1-156 | [»] | |
AlphaFoldDBi | Q16763 | |||||
BMRBi | Q16763 | |||||
SMRi | Q16763 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 118150, 125 interactors |
DIPi | DIP-52784N |
IntActi | Q16763, 23 interactors |
MINTi | Q16763 |
STRINGi | 9606.ENSP00000264552 |
PTM databases
iPTMneti | Q16763 |
MetOSitei | Q16763 |
PhosphoSitePlusi | Q16763 |
SwissPalmi | Q16763 |
Genetic variation databases
BioMutai | UBE2S |
DMDMi | 23829978 |
Proteomic databases
EPDi | Q16763 |
jPOSTi | Q16763 |
MassIVEi | Q16763 |
MaxQBi | Q16763 |
PaxDbi | Q16763 |
PeptideAtlasi | Q16763 |
PRIDEi | Q16763 |
ProteomicsDBi | 61055 |
Protocols and materials databases
Antibodypediai | 33096, 546 antibodies from 31 providers |
DNASUi | 27338 |
Genome annotation databases
Ensembli | ENST00000264552.14; ENSP00000264552.8; ENSG00000108106.14 |
GeneIDi | 27338 |
KEGGi | hsa:27338 |
MANE-Selecti | ENST00000264552.14; ENSP00000264552.8; NM_014501.3; NP_055316.2 |
UCSCi | uc002qkx.2, human |
Organism-specific databases
CTDi | 27338 |
DisGeNETi | 27338 |
GeneCardsi | UBE2S |
HGNCi | HGNC:17895, UBE2S |
HPAi | ENSG00000108106, Tissue enhanced (bone marrow, testis) |
MIMi | 610309, gene |
neXtProti | NX_Q16763 |
OpenTargetsi | ENSG00000108106 |
PharmGKBi | PA134904441 |
VEuPathDBi | HostDB:ENSG00000108106 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG0423, Eukaryota |
GeneTreei | ENSGT00940000157149 |
InParanoidi | Q16763 |
OMAi | QPAKCGA |
OrthoDBi | 1412570at2759 |
PhylomeDBi | Q16763 |
TreeFami | TF101120 |
Enzyme and pathway databases
UniPathwayi | UPA00143 |
BRENDAi | 2.3.2.24, 2681 |
PathwayCommonsi | Q16763 |
Reactomei | R-HSA-141430, Inactivation of APC/C via direct inhibition of the APC/C complex R-HSA-174048, APC/C:Cdc20 mediated degradation of Cyclin B R-HSA-174084, Autodegradation of Cdh1 by Cdh1:APC/C R-HSA-174154, APC/C:Cdc20 mediated degradation of Securin R-HSA-174178, APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1 R-HSA-174184, Cdc20:Phospho-APC/C mediated degradation of Cyclin A R-HSA-176407, Conversion from APC/C:Cdc20 to APC/C:Cdh1 in late anaphase R-HSA-176408, Regulation of APC/C activators between G1/S and early anaphase R-HSA-176409, APC/C:Cdc20 mediated degradation of mitotic proteins R-HSA-176412, Phosphorylation of the APC/C R-HSA-179409, APC-Cdc20 mediated degradation of Nek2A R-HSA-2467813, Separation of Sister Chromatids R-HSA-2559582, Senescence-Associated Secretory Phenotype (SASP) R-HSA-69017, CDK-mediated phosphorylation and removal of Cdc6 R-HSA-8853884, Transcriptional Regulation by VENTX R-HSA-8866652, Synthesis of active ubiquitin: roles of E1 and E2 enzymes R-HSA-9687136, Aberrant regulation of mitotic exit in cancer due to RB1 defects R-HSA-983168, Antigen processing: Ubiquitination & Proteasome degradation |
SignaLinki | Q16763 |
SIGNORi | Q16763 |
Miscellaneous databases
BioGRID-ORCSi | 27338, 264 hits in 1060 CRISPR screens |
ChiTaRSi | UBE2S, human |
EvolutionaryTracei | Q16763 |
GenomeRNAii | 27338 |
Pharosi | Q16763, Tbio |
PROi | PR:Q16763 |
RNActi | Q16763, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000108106, Expressed in testis and 115 other tissues |
ExpressionAtlasi | Q16763, baseline and differential |
Genevisiblei | Q16763, HS |
Family and domain databases
CDDi | cd00195, UBCc, 1 hit |
Gene3Di | 3.10.110.10, 1 hit |
InterProi | View protein in InterPro IPR000608, UBQ-conjugat_E2 IPR023313, UBQ-conjugating_AS IPR016135, UBQ-conjugating_enzyme/RWD |
Pfami | View protein in Pfam PF00179, UQ_con, 1 hit |
SUPFAMi | SSF54495, SSF54495, 1 hit |
PROSITEi | View protein in PROSITE PS00183, UBC_1, 1 hit PS50127, UBC_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | UBE2S_HUMAN | |
Accessioni | Q16763Primary (citable) accession number: Q16763 Secondary accession number(s): Q9BTC1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | October 10, 2002 | |
Last modified: | May 25, 2022 | |
This is version 199 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 19
Human chromosome 19: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families