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Protein

Cytochrome P450 2A13

Gene

CYP2A13

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Exhibits a coumarin 7-hydroxylase activity. Active in the metabolic activation of hexamethylphosphoramide, N,N-dimethylaniline, 2'-methoxyacetophenone, N-nitrosomethylphenylamine, and the tobacco-specific carcinogen, 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone. Possesses phenacetin O-deethylation activity.1 Publication

Catalytic activityi

RH + [reduced NADPH--hemoprotein reductase] + O2 = ROH + [oxidized NADPH--hemoprotein reductase] + H2O.

Cofactori

Kineticsi

  1. KM=10.7 µM for phenacetin1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Binding sitei297Substrate1
    Metal bindingi439Iron (heme axial ligand)1

    GO - Molecular functioni

    GO - Biological processi

    Keywordsi

    Molecular functionMonooxygenase, Oxidoreductase
    LigandHeme, Iron, Metal-binding

    Enzyme and pathway databases

    ReactomeiR-HSA-211935 Fatty acids
    R-HSA-211981 Xenobiotics
    R-HSA-211999 CYP2E1 reactions
    R-HSA-5423646 Aflatoxin activation and detoxification
    SABIO-RKiQ16696

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cytochrome P450 2A13 (EC:1.14.14.1)
    Alternative name(s):
    CYPIIA13
    Gene namesi
    Name:CYP2A13
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    Proteomesi
    • UP000005640 Componenti: Chromosome 19

    Organism-specific databases

    EuPathDBiHostDB:ENSG00000197838.4
    HGNCiHGNC:2608 CYP2A13
    MIMi608055 gene
    neXtProtiNX_Q16696

    Subcellular locationi

    Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane, Microsome

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Mutagenesisi110L → V: Decreases phenacetin O-deethylation activity 8 fold. 1 Publication1
    Mutagenesisi117A → V: Increases phenacetin O-deethylation activity 5 fold. 1 Publication1
    Mutagenesisi208S → I: Decreases phenacetin O-deethylation activity 10 fold. 1 Publication1
    Mutagenesisi213A → S: Decreases phenacetin O-deethylation activity 2 fold. 1 Publication1
    Mutagenesisi300F → I: Decreases phenacetin O-deethylation activity 40 fold. 1 Publication1
    Mutagenesisi301A → G: Decreases phenacetin O-deethylation activity 20 fold. 1 Publication1
    Mutagenesisi365M → V: Decreases phenacetin O-deethylation activity 7 fold. 1 Publication1
    Mutagenesisi366L → I: Increases phenacetin O-deethylation activity 3 fold. 1 Publication1
    Mutagenesisi369G → S: Decreases phenacetin O-deethylation activity 9 fold. 1 Publication1
    Mutagenesisi372H → R: Decreases phenacetin O-deethylation activity 3 fold. 1 Publication1

    Organism-specific databases

    DisGeNETi1553
    OpenTargetsiENSG00000197838
    PharmGKBiPA27101

    Chemistry databases

    ChEMBLiCHEMBL3542436
    DrugBankiDB00553 Methoxsalen
    DB00184 Nicotine
    DB03783 Phenacetin
    DB00624 Testosterone

    Polymorphism and mutation databases

    BioMutaiCYP2A13
    DMDMi77416854

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00000516761 – 494Cytochrome P450 2A13Add BLAST494

    Proteomic databases

    EPDiQ16696
    PaxDbiQ16696
    PeptideAtlasiQ16696
    PRIDEiQ16696
    ProteomicsDBi61036

    PTM databases

    iPTMnetiQ16696
    PhosphoSitePlusiQ16696

    Expressioni

    Tissue specificityi

    Expressed in liver and a number of extrahepatic tissues, including nasal mucosa, lung, trachea, brain, mammary gland, prostate, testis, and uterus, but not in heart, kidney, bone marrow, colon, small intestine, spleen, stomach, thymus, or skeletal muscle.1 Publication

    Gene expression databases

    BgeeiENSG00000197838 Expressed in 87 organ(s), highest expression level in nasal cavity epithelium
    CleanExiHS_CYP2A13
    GenevisibleiQ16696 HS

    Organism-specific databases

    HPAiHPA046713
    HPA047262

    Interactioni

    Protein-protein interaction databases

    BioGridi107931, 1 interactor
    STRINGi9606.ENSP00000332679

    Chemistry databases

    BindingDBiQ16696

    Structurei

    Secondary structure

    1494
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details

    3D structure databases

    ProteinModelPortaliQ16696
    SMRiQ16696
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ16696

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the cytochrome P450 family.Curated

    Phylogenomic databases

    eggNOGiKOG0156 Eukaryota
    COG2124 LUCA
    GeneTreeiENSGT00880000137861
    HOGENOMiHOG000036992
    HOVERGENiHBG015789
    InParanoidiQ16696
    KOiK17685
    OMAiQYLSFGC
    OrthoDBiEOG091G0BT8
    PhylomeDBiQ16696
    TreeFamiTF352043

    Family and domain databases

    Gene3Di1.10.630.10, 1 hit
    InterProiView protein in InterPro
    IPR001128 Cyt_P450
    IPR017972 Cyt_P450_CS
    IPR002401 Cyt_P450_E_grp-I
    IPR008067 Cyt_P450_E_grp-I_CYP2A-like
    IPR036396 Cyt_P450_sf
    PfamiView protein in Pfam
    PF00067 p450, 1 hit
    PRINTSiPR00463 EP450I
    PR01684 EP450ICYP2A
    PR00385 P450
    SUPFAMiSSF48264 SSF48264, 1 hit
    PROSITEiView protein in PROSITE
    PS00086 CYTOCHROME_P450, 1 hit

    Sequencei

    Sequence statusi: Complete.

    Q16696-1 [UniParc]FASTAAdd to basket
    « Hide
            10         20         30         40         50
    MLASGLLLVT LLACLTVMVL MSVWRQRKSR GKLPPGPTPL PFIGNYLQLN
    60 70 80 90 100
    TEQMYNSLMK ISERYGPVFT IHLGPRRVVV LCGHDAVKEA LVDQAEEFSG
    110 120 130 140 150
    RGEQATFDWL FKGYGVAFSN GERAKQLRRF SIATLRGFGV GKRGIEERIQ
    160 170 180 190 200
    EEAGFLIDAL RGTHGANIDP TFFLSRTVSN VISSIVFGDR FDYEDKEFLS
    210 220 230 240 250
    LLRMMLGSFQ FTATSTGQLY EMFSSVMKHL PGPQQQAFKE LQGLEDFIAK
    260 270 280 290 300
    KVEHNQRTLD PNSPRDFIDS FLIRMQEEEK NPNTEFYLKN LVMTTLNLFF
    310 320 330 340 350
    AGTETVSTTL RYGFLLLMKH PEVEAKVHEE IDRVIGKNRQ PKFEDRAKMP
    360 370 380 390 400
    YTEAVIHEIQ RFGDMLPMGL AHRVNKDTKF RDFFLPKGTE VFPMLGSVLR
    410 420 430 440 450
    DPRFFSNPRD FNPQHFLDKK GQFKKSDAFV PFSIGKRYCF GEGLARMELF
    460 470 480 490
    LFFTTIMQNF RFKSPQSPKD IDVSPKHVGF ATIPRNYTMS FLPR
    Length:494
    Mass (Da):56,688
    Last modified:October 11, 2005 - v3
    Checksum:iA39F18AD71C28821
    GO

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti208S → R in AAB40519 (PubMed:7668294).Curated1
    Sequence conflicti213A → G in AAB40519 (PubMed:7668294).Curated1
    Sequence conflicti398V → E in AAB40519 (PubMed:7668294).Curated1
    Sequence conflicti409 – 412RDFN → QDCS in AAB40519 (PubMed:7668294).Curated4
    Sequence conflicti419K → E in AAB40519 (PubMed:7668294).Curated1

    Polymorphismi

    The frequencies of the Cys-257 allele in white, black, Hispanic, and Asian individuals are 1.9%, 14.4%, 5.8%, and 7.7%, respectively. The Cys-257 variant is 37 to 56% less active than the wild-type Arg-257 protein toward all substrates tested.

    Natural variant

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Natural variantiVAR_01833425R → Q in allele CYP2A13*2. 2 PublicationsCorresponds to variant dbSNP:rs8192784Ensembl.1
    Natural variantiVAR_018335101R → Q in allele CYP2A13*4. 1 PublicationCorresponds to variant dbSNP:rs148044792Ensembl.1
    Natural variantiVAR_018336134T → TT in allele CYP2A13*3. 1 Publication1
    Natural variantiVAR_018337158D → E in allele CYP2A13*3 and allele CYP2A13*8. 2 PublicationsCorresponds to variant dbSNP:rs112337232Ensembl.1
    Natural variantiVAR_013835257R → C in allele CYP2A13*2. 2 PublicationsCorresponds to variant dbSNP:rs8192789Ensembl.1
    Natural variantiVAR_018356323V → L in allele CYP2A13*9. 1 Publication1
    Natural variantiVAR_018338453F → Y in allele CYP2A13*5. 1 PublicationCorresponds to variant dbSNP:rs72547590Ensembl.1
    Natural variantiVAR_018339494R → C in allele CYP2A13*6. 1 PublicationCorresponds to variant dbSNP:rs138870349Ensembl.1

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    U22028 Genomic DNA Translation: AAB40519.1
    AF209774 mRNA Translation: AAG35775.1
    AY513604 mRNA Translation: AAR90935.1
    AY513605 mRNA Translation: AAR90936.1
    AY513606 mRNA Translation: AAR90937.1
    AY513608 mRNA Translation: AAR90939.1
    AY513609 mRNA Translation: AAR90940.1
    CCDSiCCDS12571.1
    PIRiI38966
    RefSeqiNP_000757.2, NM_000766.4
    UniGeneiHs.567252

    Genome annotation databases

    EnsembliENST00000330436; ENSP00000332679; ENSG00000197838
    GeneIDi1553
    KEGGihsa:1553
    UCSCiuc002opt.5 human

    Keywords - Coding sequence diversityi

    Polymorphism

    Similar proteinsi

    Cross-referencesi

    Web resourcesi

    Cytochrome P450 Allele Nomenclature Committee

    CYP2A13 alleles

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    U22028 Genomic DNA Translation: AAB40519.1
    AF209774 mRNA Translation: AAG35775.1
    AY513604 mRNA Translation: AAR90935.1
    AY513605 mRNA Translation: AAR90936.1
    AY513606 mRNA Translation: AAR90937.1
    AY513608 mRNA Translation: AAR90939.1
    AY513609 mRNA Translation: AAR90940.1
    CCDSiCCDS12571.1
    PIRiI38966
    RefSeqiNP_000757.2, NM_000766.4
    UniGeneiHs.567252

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    2P85X-ray2.35A/B/C/D/E/F26-494[»]
    2PG5X-ray1.95A/B/C/D31-494[»]
    2PG6X-ray2.53A/B/C/D31-494[»]
    2PG7X-ray2.80A/B/C/D31-494[»]
    3T3SX-ray3.00A/B/C/D/E/F/G/H31-494[»]
    4EJGX-ray2.50A/B/C/D/E/F/G/H31-494[»]
    4EJHX-ray2.35A/B/C/D/E/F/G/H31-494[»]
    4EJIX-ray2.10A31-494[»]
    ProteinModelPortaliQ16696
    SMRiQ16696
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi107931, 1 interactor
    STRINGi9606.ENSP00000332679

    Chemistry databases

    BindingDBiQ16696
    ChEMBLiCHEMBL3542436
    DrugBankiDB00553 Methoxsalen
    DB00184 Nicotine
    DB03783 Phenacetin
    DB00624 Testosterone

    PTM databases

    iPTMnetiQ16696
    PhosphoSitePlusiQ16696

    Polymorphism and mutation databases

    BioMutaiCYP2A13
    DMDMi77416854

    Proteomic databases

    EPDiQ16696
    PaxDbiQ16696
    PeptideAtlasiQ16696
    PRIDEiQ16696
    ProteomicsDBi61036

    Protocols and materials databases

    DNASUi1553
    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsembliENST00000330436; ENSP00000332679; ENSG00000197838
    GeneIDi1553
    KEGGihsa:1553
    UCSCiuc002opt.5 human

    Organism-specific databases

    CTDi1553
    DisGeNETi1553
    EuPathDBiHostDB:ENSG00000197838.4
    GeneCardsiCYP2A13
    HGNCiHGNC:2608 CYP2A13
    HPAiHPA046713
    HPA047262
    MIMi608055 gene
    neXtProtiNX_Q16696
    OpenTargetsiENSG00000197838
    PharmGKBiPA27101
    GenAtlasiSearch...

    Phylogenomic databases

    eggNOGiKOG0156 Eukaryota
    COG2124 LUCA
    GeneTreeiENSGT00880000137861
    HOGENOMiHOG000036992
    HOVERGENiHBG015789
    InParanoidiQ16696
    KOiK17685
    OMAiQYLSFGC
    OrthoDBiEOG091G0BT8
    PhylomeDBiQ16696
    TreeFamiTF352043

    Enzyme and pathway databases

    ReactomeiR-HSA-211935 Fatty acids
    R-HSA-211981 Xenobiotics
    R-HSA-211999 CYP2E1 reactions
    R-HSA-5423646 Aflatoxin activation and detoxification
    SABIO-RKiQ16696

    Miscellaneous databases

    EvolutionaryTraceiQ16696
    GeneWikiiCYP2A13
    GenomeRNAii1553
    PROiPR:Q16696
    SOURCEiSearch...

    Gene expression databases

    BgeeiENSG00000197838 Expressed in 87 organ(s), highest expression level in nasal cavity epithelium
    CleanExiHS_CYP2A13
    GenevisibleiQ16696 HS

    Family and domain databases

    Gene3Di1.10.630.10, 1 hit
    InterProiView protein in InterPro
    IPR001128 Cyt_P450
    IPR017972 Cyt_P450_CS
    IPR002401 Cyt_P450_E_grp-I
    IPR008067 Cyt_P450_E_grp-I_CYP2A-like
    IPR036396 Cyt_P450_sf
    PfamiView protein in Pfam
    PF00067 p450, 1 hit
    PRINTSiPR00463 EP450I
    PR01684 EP450ICYP2A
    PR00385 P450
    SUPFAMiSSF48264 SSF48264, 1 hit
    PROSITEiView protein in PROSITE
    PS00086 CYTOCHROME_P450, 1 hit
    ProtoNetiSearch...

    Entry informationi

    Entry nameiCP2AD_HUMAN
    AccessioniPrimary (citable) accession number: Q16696
    Secondary accession number(s): Q53YR8
    , Q6R569, Q6R570, Q9H2X2
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 15, 1998
    Last sequence update: October 11, 2005
    Last modified: November 7, 2018
    This is version 174 of the entry and version 3 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. Human chromosome 19
      Human chromosome 19: entries, gene names and cross-references to MIM
    6. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    UniProt is an ELIXIR core data resource
    Main funding by: National Institutes of Health

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