UniProtKB - Q16696 (CP2AD_HUMAN)
Protein
Cytochrome P450 2A13
Gene
CYP2A13
Organism
Homo sapiens (Human)
Status
Functioni
Exhibits a coumarin 7-hydroxylase activity. Active in the metabolic activation of hexamethylphosphoramide, N,N-dimethylaniline, 2'-methoxyacetophenone, N-nitrosomethylphenylamine, and the tobacco-specific carcinogen, 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone. Possesses phenacetin O-deethylation activity.1 Publication
Catalytic activityi
- an organic molecule + O2 + reduced [NADPH—hemoprotein reductase] = an alcohol + H+ + H2O + oxidized [NADPH—hemoprotein reductase]EC:1.14.14.1
Cofactori
Kineticsi
- KM=10.7 µM for phenacetin1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 297 | Substrate | 1 | |
Metal bindingi | 439 | Iron (heme axial ligand) | 1 |
GO - Molecular functioni
- arachidonic acid epoxygenase activity Source: GO_Central
- aromatase activity Source: UniProtKB-EC
- heme binding Source: UniProtKB
- iron ion binding Source: InterPro
- monooxygenase activity Source: Reactome
- oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen Source: GO_Central
- steroid hydroxylase activity Source: GO_Central
GO - Biological processi
- coumarin metabolic process Source: GO_Central
- epoxygenase P450 pathway Source: GO_Central
- exogenous drug catabolic process Source: GO_Central
- organic acid metabolic process Source: GO_Central
- oxidation-reduction process Source: GO_Central
- xenobiotic metabolic process Source: GO_Central
Keywordsi
Molecular function | Monooxygenase, Oxidoreductase |
Ligand | Heme, Iron, Metal-binding |
Enzyme and pathway databases
PathwayCommonsi | Q16696 |
Reactomei | R-HSA-211935, Fatty acids R-HSA-211981, Xenobiotics R-HSA-211999, CYP2E1 reactions R-HSA-5423646, Aflatoxin activation and detoxification |
SABIO-RKi | Q16696 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:CYP2A13 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
EuPathDBi | HostDB:ENSG00000197838.4 |
HGNCi | HGNC:2608, CYP2A13 |
MIMi | 608055, gene |
neXtProti | NX_Q16696 |
Subcellular locationi
Endoplasmic reticulum
Endoplasmic reticulum
- endoplasmic reticulum membrane Source: Reactome
Other locations
- cytoplasm Source: GO_Central
- intracellular membrane-bounded organelle Source: GO_Central
Keywords - Cellular componenti
Endoplasmic reticulum, Membrane, MicrosomePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 110 | L → V: Decreases phenacetin O-deethylation activity 8 fold. 1 Publication | 1 | |
Mutagenesisi | 117 | A → V: Increases phenacetin O-deethylation activity 5 fold. 1 Publication | 1 | |
Mutagenesisi | 208 | S → I: Decreases phenacetin O-deethylation activity 10 fold. 1 Publication | 1 | |
Mutagenesisi | 213 | A → S: Decreases phenacetin O-deethylation activity 2 fold. 1 Publication | 1 | |
Mutagenesisi | 300 | F → I: Decreases phenacetin O-deethylation activity 40 fold. 1 Publication | 1 | |
Mutagenesisi | 301 | A → G: Decreases phenacetin O-deethylation activity 20 fold. 1 Publication | 1 | |
Mutagenesisi | 365 | M → V: Decreases phenacetin O-deethylation activity 7 fold. 1 Publication | 1 | |
Mutagenesisi | 366 | L → I: Increases phenacetin O-deethylation activity 3 fold. 1 Publication | 1 | |
Mutagenesisi | 369 | G → S: Decreases phenacetin O-deethylation activity 9 fold. 1 Publication | 1 | |
Mutagenesisi | 372 | H → R: Decreases phenacetin O-deethylation activity 3 fold. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 1553 |
OpenTargetsi | ENSG00000197838 |
PharmGKBi | PA27101 |
Miscellaneous databases
Pharosi | Q16696, Tbio |
Chemistry databases
ChEMBLi | CHEMBL3542436 |
DrugBanki | DB00553, Methoxsalen DB00184, Nicotine DB03783, Phenacetin DB00624, Testosterone DB13943, Testosterone cypionate DB13944, Testosterone enanthate DB13946, Testosterone undecanoate |
Polymorphism and mutation databases
BioMutai | CYP2A13 |
DMDMi | 77416854 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000051676 | 1 – 494 | Cytochrome P450 2A13Add BLAST | 494 |
Proteomic databases
EPDi | Q16696 |
MassIVEi | Q16696 |
PaxDbi | Q16696 |
PeptideAtlasi | Q16696 |
PRIDEi | Q16696 |
ProteomicsDBi | 61036 |
PTM databases
iPTMneti | Q16696 |
PhosphoSitePlusi | Q16696 |
Expressioni
Tissue specificityi
Expressed in liver and a number of extrahepatic tissues, including nasal mucosa, lung, trachea, brain, mammary gland, prostate, testis, and uterus, but not in heart, kidney, bone marrow, colon, small intestine, spleen, stomach, thymus, or skeletal muscle.1 Publication
Gene expression databases
Bgeei | ENSG00000197838, Expressed in nasal cavity epithelium and 90 other tissues |
Genevisiblei | Q16696, HS |
Organism-specific databases
HPAi | ENSG00000197838, Tissue enriched (liver) |
Interactioni
Protein-protein interaction databases
BioGRIDi | 107931, 1 interactor |
IntActi | Q16696, 1 interactor |
STRINGi | 9606.ENSP00000332679 |
Chemistry databases
BindingDBi | Q16696 |
Miscellaneous databases
RNActi | Q16696, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q16696 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q16696 |
Family & Domainsi
Sequence similaritiesi
Belongs to the cytochrome P450 family.Curated
Phylogenomic databases
eggNOGi | KOG0156, Eukaryota |
GeneTreei | ENSGT00940000154117 |
HOGENOMi | CLU_001570_22_3_1 |
InParanoidi | Q16696 |
OMAi | TQQMYNS |
OrthoDBi | 702827at2759 |
PhylomeDBi | Q16696 |
TreeFami | TF352043 |
Family and domain databases
Gene3Di | 1.10.630.10, 1 hit |
InterProi | View protein in InterPro IPR001128, Cyt_P450 IPR017972, Cyt_P450_CS IPR002401, Cyt_P450_E_grp-I IPR008067, Cyt_P450_E_grp-I_CYP2A-like IPR036396, Cyt_P450_sf |
Pfami | View protein in Pfam PF00067, p450, 1 hit |
PRINTSi | PR00463, EP450I PR01684, EP450ICYP2A PR00385, P450 |
SUPFAMi | SSF48264, SSF48264, 1 hit |
PROSITEi | View protein in PROSITE PS00086, CYTOCHROME_P450, 1 hit |
i Sequence
Sequence statusi: Complete.
Q16696-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MLASGLLLVT LLACLTVMVL MSVWRQRKSR GKLPPGPTPL PFIGNYLQLN
60 70 80 90 100
TEQMYNSLMK ISERYGPVFT IHLGPRRVVV LCGHDAVKEA LVDQAEEFSG
110 120 130 140 150
RGEQATFDWL FKGYGVAFSN GERAKQLRRF SIATLRGFGV GKRGIEERIQ
160 170 180 190 200
EEAGFLIDAL RGTHGANIDP TFFLSRTVSN VISSIVFGDR FDYEDKEFLS
210 220 230 240 250
LLRMMLGSFQ FTATSTGQLY EMFSSVMKHL PGPQQQAFKE LQGLEDFIAK
260 270 280 290 300
KVEHNQRTLD PNSPRDFIDS FLIRMQEEEK NPNTEFYLKN LVMTTLNLFF
310 320 330 340 350
AGTETVSTTL RYGFLLLMKH PEVEAKVHEE IDRVIGKNRQ PKFEDRAKMP
360 370 380 390 400
YTEAVIHEIQ RFGDMLPMGL AHRVNKDTKF RDFFLPKGTE VFPMLGSVLR
410 420 430 440 450
DPRFFSNPRD FNPQHFLDKK GQFKKSDAFV PFSIGKRYCF GEGLARMELF
460 470 480 490
LFFTTIMQNF RFKSPQSPKD IDVSPKHVGF ATIPRNYTMS FLPR
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 208 | S → R in AAB40519 (PubMed:7668294).Curated | 1 | |
Sequence conflicti | 213 | A → G in AAB40519 (PubMed:7668294).Curated | 1 | |
Sequence conflicti | 398 | V → E in AAB40519 (PubMed:7668294).Curated | 1 | |
Sequence conflicti | 409 – 412 | RDFN → QDCS in AAB40519 (PubMed:7668294).Curated | 4 | |
Sequence conflicti | 419 | K → E in AAB40519 (PubMed:7668294).Curated | 1 |
Polymorphismi
The frequencies of the Cys-257 allele in white, black, Hispanic, and Asian individuals are 1.9%, 14.4%, 5.8%, and 7.7%, respectively. The Cys-257 variant is 37 to 56% less active than the wild-type Arg-257 protein toward all substrates tested.
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_018334 | 25 | R → Q in allele CYP2A13*2. 2 PublicationsCorresponds to variant dbSNP:rs8192784Ensembl. | 1 | |
Natural variantiVAR_018335 | 101 | R → Q in allele CYP2A13*4. 1 PublicationCorresponds to variant dbSNP:rs148044792Ensembl. | 1 | |
Natural variantiVAR_018336 | 134 | T → TT in allele CYP2A13*3. 1 Publication | 1 | |
Natural variantiVAR_018337 | 158 | D → E in allele CYP2A13*3 and allele CYP2A13*8. 2 PublicationsCorresponds to variant dbSNP:rs112337232Ensembl. | 1 | |
Natural variantiVAR_013835 | 257 | R → C in allele CYP2A13*2. 2 PublicationsCorresponds to variant dbSNP:rs8192789Ensembl. | 1 | |
Natural variantiVAR_018356 | 323 | V → L in allele CYP2A13*9. 1 Publication | 1 | |
Natural variantiVAR_018338 | 453 | F → Y in allele CYP2A13*5. 1 PublicationCorresponds to variant dbSNP:rs72547590Ensembl. | 1 | |
Natural variantiVAR_018339 | 494 | R → C in allele CYP2A13*6. 1 PublicationCorresponds to variant dbSNP:rs138870349Ensembl. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U22028 Genomic DNA Translation: AAB40519.1 AF209774 mRNA Translation: AAG35775.1 AY513604 mRNA Translation: AAR90935.1 AY513605 mRNA Translation: AAR90936.1 AY513606 mRNA Translation: AAR90937.1 AY513608 mRNA Translation: AAR90939.1 AY513609 mRNA Translation: AAR90940.1 |
CCDSi | CCDS12571.1 |
PIRi | I38966 |
RefSeqi | NP_000757.2, NM_000766.4 |
Genome annotation databases
Ensembli | ENST00000330436; ENSP00000332679; ENSG00000197838 |
GeneIDi | 1553 |
KEGGi | hsa:1553 |
UCSCi | uc002opt.5, human |
Keywords - Coding sequence diversityi
PolymorphismSimilar proteinsi
Cross-referencesi
Web resourcesi
Cytochrome P450 Allele Nomenclature Committee CYP2A13 alleles |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U22028 Genomic DNA Translation: AAB40519.1 AF209774 mRNA Translation: AAG35775.1 AY513604 mRNA Translation: AAR90935.1 AY513605 mRNA Translation: AAR90936.1 AY513606 mRNA Translation: AAR90937.1 AY513608 mRNA Translation: AAR90939.1 AY513609 mRNA Translation: AAR90940.1 |
CCDSi | CCDS12571.1 |
PIRi | I38966 |
RefSeqi | NP_000757.2, NM_000766.4 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2P85 | X-ray | 2.35 | A/B/C/D/E/F | 26-494 | [»] | |
2PG5 | X-ray | 1.95 | A/B/C/D | 31-494 | [»] | |
2PG6 | X-ray | 2.53 | A/B/C/D | 31-494 | [»] | |
2PG7 | X-ray | 2.80 | A/B/C/D | 31-494 | [»] | |
3T3S | X-ray | 3.00 | A/B/C/D/E/F/G/H | 31-494 | [»] | |
4EJG | X-ray | 2.50 | A/B/C/D/E/F/G/H | 31-494 | [»] | |
4EJH | X-ray | 2.35 | A/B/C/D/E/F/G/H | 31-494 | [»] | |
4EJI | X-ray | 2.10 | A | 31-494 | [»] | |
SMRi | Q16696 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 107931, 1 interactor |
IntActi | Q16696, 1 interactor |
STRINGi | 9606.ENSP00000332679 |
Chemistry databases
BindingDBi | Q16696 |
ChEMBLi | CHEMBL3542436 |
DrugBanki | DB00553, Methoxsalen DB00184, Nicotine DB03783, Phenacetin DB00624, Testosterone DB13943, Testosterone cypionate DB13944, Testosterone enanthate DB13946, Testosterone undecanoate |
PTM databases
iPTMneti | Q16696 |
PhosphoSitePlusi | Q16696 |
Polymorphism and mutation databases
BioMutai | CYP2A13 |
DMDMi | 77416854 |
Proteomic databases
EPDi | Q16696 |
MassIVEi | Q16696 |
PaxDbi | Q16696 |
PeptideAtlasi | Q16696 |
PRIDEi | Q16696 |
ProteomicsDBi | 61036 |
Protocols and materials databases
Antibodypediai | 17121, 163 antibodies |
DNASUi | 1553 |
Genome annotation databases
Ensembli | ENST00000330436; ENSP00000332679; ENSG00000197838 |
GeneIDi | 1553 |
KEGGi | hsa:1553 |
UCSCi | uc002opt.5, human |
Organism-specific databases
CTDi | 1553 |
DisGeNETi | 1553 |
EuPathDBi | HostDB:ENSG00000197838.4 |
GeneCardsi | CYP2A13 |
HGNCi | HGNC:2608, CYP2A13 |
HPAi | ENSG00000197838, Tissue enriched (liver) |
MIMi | 608055, gene |
neXtProti | NX_Q16696 |
OpenTargetsi | ENSG00000197838 |
PharmGKBi | PA27101 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG0156, Eukaryota |
GeneTreei | ENSGT00940000154117 |
HOGENOMi | CLU_001570_22_3_1 |
InParanoidi | Q16696 |
OMAi | TQQMYNS |
OrthoDBi | 702827at2759 |
PhylomeDBi | Q16696 |
TreeFami | TF352043 |
Enzyme and pathway databases
PathwayCommonsi | Q16696 |
Reactomei | R-HSA-211935, Fatty acids R-HSA-211981, Xenobiotics R-HSA-211999, CYP2E1 reactions R-HSA-5423646, Aflatoxin activation and detoxification |
SABIO-RKi | Q16696 |
Miscellaneous databases
BioGRID-ORCSi | 1553, 153 hits in 841 CRISPR screens |
EvolutionaryTracei | Q16696 |
GeneWikii | CYP2A13 |
GenomeRNAii | 1553 |
Pharosi | Q16696, Tbio |
PROi | PR:Q16696 |
RNActi | Q16696, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000197838, Expressed in nasal cavity epithelium and 90 other tissues |
Genevisiblei | Q16696, HS |
Family and domain databases
Gene3Di | 1.10.630.10, 1 hit |
InterProi | View protein in InterPro IPR001128, Cyt_P450 IPR017972, Cyt_P450_CS IPR002401, Cyt_P450_E_grp-I IPR008067, Cyt_P450_E_grp-I_CYP2A-like IPR036396, Cyt_P450_sf |
Pfami | View protein in Pfam PF00067, p450, 1 hit |
PRINTSi | PR00463, EP450I PR01684, EP450ICYP2A PR00385, P450 |
SUPFAMi | SSF48264, SSF48264, 1 hit |
PROSITEi | View protein in PROSITE PS00086, CYTOCHROME_P450, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CP2AD_HUMAN | |
Accessioni | Q16696Primary (citable) accession number: Q16696 Secondary accession number(s): Q53YR8 Q9H2X2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 15, 1998 |
Last sequence update: | October 11, 2005 | |
Last modified: | December 2, 2020 | |
This is version 187 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human polymorphisms and disease mutations
Index of human polymorphisms and disease mutations - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families - Human chromosome 19
Human chromosome 19: entries, gene names and cross-references to MIM - Human entries with polymorphisms or disease mutations
List of human entries with polymorphisms or disease mutations