UniProtKB - Q16656 (NRF1_HUMAN)
Protein
Nuclear respiratory factor 1
Gene
NRF1
Organism
Homo sapiens (Human)
Status
Functioni
Transcription factor that activates the expression of the EIF2S1 (EIF2-alpha) gene. Links the transcriptional modulation of key metabolic genes to cellular growth and development. Implicated in the control of nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication.
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
DNA bindingi | 109 – 305 | Add BLAST | 197 |
GO - Molecular functioni
- core promoter sequence-specific DNA binding Source: AgBase
- DNA-binding transcription activator activity, RNA polymerase II-specific Source: NTNU_SB
- protein homodimerization activity Source: AgBase
- RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: NTNU_SB
- RNA polymerase II core promoter sequence-specific DNA binding Source: ARUK-UCL
GO - Biological processi
- generation of precursor metabolites and energy Source: ProtInc
- mitochondrion organization Source: Reactome
- positive regulation of transcription by RNA polymerase II Source: NTNU_SB
- regulation of transcription by RNA polymerase II Source: GO_Central
Keywordsi
Molecular function | Activator, DNA-binding |
Biological process | Transcription, Transcription regulation |
Enzyme and pathway databases
PathwayCommonsi | Q16656 |
Reactomei | R-HSA-1989781, PPARA activates gene expression R-HSA-2151201, Transcriptional activation of mitochondrial biogenesis |
SignaLinki | Q16656 |
SIGNORi | Q16656 |
Names & Taxonomyi
Protein namesi | Recommended name: Nuclear respiratory factor 1Short name: NRF-1 Alternative name(s): Alpha palindromic-binding protein Short name: Alpha-pal |
Gene namesi | Name:NRF1 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
EuPathDBi | HostDB:ENSG00000106459.14 |
HGNCi | HGNC:7996, NRF1 |
MIMi | 600879, gene |
neXtProti | NX_Q16656 |
Subcellular locationi
Nucleus
Cytosol
- cytosol Source: HPA
Extracellular region or secreted
- extracellular exosome Source: UniProtKB
Nucleus
- nucleoplasm Source: HPA
- nucleus Source: AgBase
Keywords - Cellular componenti
NucleusPathology & Biotechi
Organism-specific databases
DisGeNETi | 4899 |
OpenTargetsi | ENSG00000106459 |
PharmGKBi | PA31775 |
Miscellaneous databases
Pharosi | Q16656, Tbio |
Polymorphism and mutation databases
BioMutai | NRF1 |
DMDMi | 12643732 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000100208 | 1 – 503 | Nuclear respiratory factor 1Add BLAST | 503 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 39 | Phosphoserine; by CK21 Publication | 1 | |
Modified residuei | 44 | Phosphoserine; by CK21 Publication | 1 | |
Modified residuei | 46 | Phosphoserine; by CK21 Publication | 1 | |
Modified residuei | 47 | Phosphoserine; by CK21 Publication | 1 | |
Modified residuei | 52 | Phosphoserine; by CK21 Publication | 1 | |
Cross-linki | 139 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources |
Post-translational modificationi
Phosphorylation enhances DNA binding.1 Publication
Keywords - PTMi
Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
EPDi | Q16656 |
jPOSTi | Q16656 |
MassIVEi | Q16656 |
MaxQBi | Q16656 |
PaxDbi | Q16656 |
PeptideAtlasi | Q16656 |
PRIDEi | Q16656 |
ProteomicsDBi | 3895 61015 [Q16656-1] 61016 [Q16656-2] 75975 |
PTM databases
GlyGeni | Q16656, 1 site, 1 O-linked glycan (1 site) |
iPTMneti | Q16656 |
PhosphoSitePlusi | Q16656 |
Expressioni
Tissue specificityi
Ubiquitously expressed with strongest expression in skeletal muscle.
Gene expression databases
Bgeei | ENSG00000106459, Expressed in corpus callosum and 194 other tissues |
ExpressionAtlasi | Q16656, baseline and differential |
Genevisiblei | Q16656, HS |
Organism-specific databases
HPAi | ENSG00000106459, Low tissue specificity |
Interactioni
Subunit structurei
Homodimer. Binds DNA as a dimer.
Interacts with PPRC1.
2 PublicationsBinary interactionsi
Hide detailsQ16656
Isoform 4 [Q16656-4]
GO - Molecular functioni
- protein homodimerization activity Source: AgBase
Protein-protein interaction databases
BioGRIDi | 110955, 61 interactors |
DIPi | DIP-56958N |
IntActi | Q16656, 51 interactors |
MINTi | Q16656 |
STRINGi | 9606.ENSP00000376924 |
Miscellaneous databases
RNActi | Q16656, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 78 | DimerizationAdd BLAST | 78 | |
Regioni | 301 – 476 | Required for transcriptional activationAdd BLAST | 176 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 88 – 116 | Nuclear localization signalAdd BLAST | 29 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 41 – 66 | Asp/Glu-rich (acidic)Add BLAST | 26 | |
Compositional biasi | 80 – 86 | Poly-Ala | 7 |
Sequence similaritiesi
Belongs to the NRF1/Ewg family.Curated
Phylogenomic databases
eggNOGi | ENOG502QTK1, Eukaryota |
GeneTreei | ENSGT00390000006835 |
HOGENOMi | CLU_018156_3_1_1 |
InParanoidi | Q16656 |
OMAi | DHTVHQT |
PhylomeDBi | Q16656 |
TreeFami | TF105308 |
Family and domain databases
InterProi | View protein in InterPro IPR039142, NRF1/Ewg IPR019526, Nrf1_activation-bd IPR019525, Nrf1_NLS/DNA-bd_dimer |
PANTHERi | PTHR20338, PTHR20338, 1 hit |
Pfami | View protein in Pfam PF10492, Nrf1_activ_bdg, 1 hit PF10491, Nrf1_DNA-bind, 1 hit |
s (4+)i Sequence
Sequence statusi: Complete.
This entry describes 4 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 4 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All
Isoform Long (identifier: Q16656-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MEEHGVTQTE HMATIEAHAV AQQVQQVHVA TYTEHSMLSA DEDSPSSPED
60 70 80 90 100
TSYDDSDILN STAADEVTAH LAAAGPVGMA AAAAVATGKK RKRPHVFESN
110 120 130 140 150
PSIRKRQQTR LLRKLRATLD EYTTRVGQQA IVLCISPSKP NPVFKVFGAA
160 170 180 190 200
PLENVVRKYK SMILEDLESA LAEHAPAPQE VNSELPPLTI DGIPVSVDKM
210 220 230 240 250
TQAQLRAFIP EMLKYSTGRG KPGWGKESCK PIWWPEDIPW ANVRSDVRTE
260 270 280 290 300
EQKQRVSWTQ ALRTIVKNCY KQHGREDLLY AFEDQQTQTQ ATATHSIAHL
310 320 330 340 350
VPSQTVVQTF SNPDGTVSLI QVGTGATVAT LADASELPTT VTVAQVNYSA
360 370 380 390 400
VADGEVEQNW ATLQGGEMTI QTTQASEATQ AVASLAEAAV AASQEMQQGA
410 420 430 440 450
TVTMALNSEA AAHAVATLAE ATLQGGGQIV LSGETAAAVG ALTGVQDANG
460 470 480 490 500
LVQIPVSMYQ TVVTSLAQGN GPVQVAMAPV TTRISDSAVT MDGQAVEVVT
LEQ
Isoform Short (identifier: Q16656-2) [UniParc]FASTAAdd to basket
The sequence of this isoform differs from the canonical sequence as follows:
256-321: Missing.
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketC9JP85 | C9JP85_HUMAN | Nuclear respiratory factor 1 | NRF1 | 209 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 322 | V → VV in AAA79013 (PubMed:7629110).Curated | 1 | |
Sequence conflicti | 403 | T → A in BAG56867 (PubMed:14702039).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_054330 | 1 – 161 | Missing in isoform 3. 1 PublicationAdd BLAST | 161 | |
Alternative sequenceiVSP_003598 | 256 – 321 | Missing in isoform Short. CuratedAdd BLAST | 66 | |
Alternative sequenceiVSP_054331 | 449 | N → NGLFMADRAGRKWILTDKAT in isoform 4. 2 Publications | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L22454 mRNA Translation: AAA16918.1 U02683 mRNA Translation: AAA21647.1 U18383 , U18375, U18376, U18377, U18378, U18379, U18380, U18381, U18382 Genomic DNA Translation: AAA79013.1 EU159452 mRNA Translation: ABV90872.1 AK290891 mRNA Translation: BAF83580.1 AK293351 mRNA Translation: BAG56867.1 AC078846 Genomic DNA No translation available. AC084864 Genomic DNA No translation available. CH236950 Genomic DNA Translation: EAL24100.1 CH471070 Genomic DNA Translation: EAW83728.1 BC016925 mRNA Translation: AAH16925.1 |
CCDSi | CCDS5813.2 [Q16656-1] CCDS78273.1 [Q16656-4] |
PIRi | A54868 |
RefSeqi | NP_001035199.1, NM_001040110.1 [Q16656-1] NP_001280092.1, NM_001293163.1 [Q16656-4] NP_001280093.1, NM_001293164.1 [Q16656-3] NP_005002.3, NM_005011.4 [Q16656-1] |
Genome annotation databases
Ensembli | ENST00000223190; ENSP00000223190; ENSG00000106459 [Q16656-1] ENST00000311967; ENSP00000309826; ENSG00000106459 [Q16656-4] ENST00000353868; ENSP00000342351; ENSG00000106459 [Q16656-4] ENST00000393230; ENSP00000376922; ENSG00000106459 [Q16656-1] ENST00000393232; ENSP00000376924; ENSG00000106459 [Q16656-1] |
GeneIDi | 4899 |
KEGGi | hsa:4899 |
UCSCi | uc003voz.4, human [Q16656-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Web resourcesi
Atlas of Genetics and Cytogenetics in Oncology and Haematology |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L22454 mRNA Translation: AAA16918.1 U02683 mRNA Translation: AAA21647.1 U18383 , U18375, U18376, U18377, U18378, U18379, U18380, U18381, U18382 Genomic DNA Translation: AAA79013.1 EU159452 mRNA Translation: ABV90872.1 AK290891 mRNA Translation: BAF83580.1 AK293351 mRNA Translation: BAG56867.1 AC078846 Genomic DNA No translation available. AC084864 Genomic DNA No translation available. CH236950 Genomic DNA Translation: EAL24100.1 CH471070 Genomic DNA Translation: EAW83728.1 BC016925 mRNA Translation: AAH16925.1 |
CCDSi | CCDS5813.2 [Q16656-1] CCDS78273.1 [Q16656-4] |
PIRi | A54868 |
RefSeqi | NP_001035199.1, NM_001040110.1 [Q16656-1] NP_001280092.1, NM_001293163.1 [Q16656-4] NP_001280093.1, NM_001293164.1 [Q16656-3] NP_005002.3, NM_005011.4 [Q16656-1] |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 110955, 61 interactors |
DIPi | DIP-56958N |
IntActi | Q16656, 51 interactors |
MINTi | Q16656 |
STRINGi | 9606.ENSP00000376924 |
PTM databases
GlyGeni | Q16656, 1 site, 1 O-linked glycan (1 site) |
iPTMneti | Q16656 |
PhosphoSitePlusi | Q16656 |
Polymorphism and mutation databases
BioMutai | NRF1 |
DMDMi | 12643732 |
Proteomic databases
EPDi | Q16656 |
jPOSTi | Q16656 |
MassIVEi | Q16656 |
MaxQBi | Q16656 |
PaxDbi | Q16656 |
PeptideAtlasi | Q16656 |
PRIDEi | Q16656 |
ProteomicsDBi | 3895 61015 [Q16656-1] 61016 [Q16656-2] 75975 |
Protocols and materials databases
Antibodypediai | 17908, 443 antibodies |
DNASUi | 4899 |
Genome annotation databases
Ensembli | ENST00000223190; ENSP00000223190; ENSG00000106459 [Q16656-1] ENST00000311967; ENSP00000309826; ENSG00000106459 [Q16656-4] ENST00000353868; ENSP00000342351; ENSG00000106459 [Q16656-4] ENST00000393230; ENSP00000376922; ENSG00000106459 [Q16656-1] ENST00000393232; ENSP00000376924; ENSG00000106459 [Q16656-1] |
GeneIDi | 4899 |
KEGGi | hsa:4899 |
UCSCi | uc003voz.4, human [Q16656-1] |
Organism-specific databases
CTDi | 4899 |
DisGeNETi | 4899 |
EuPathDBi | HostDB:ENSG00000106459.14 |
GeneCardsi | NRF1 |
HGNCi | HGNC:7996, NRF1 |
HPAi | ENSG00000106459, Low tissue specificity |
MIMi | 600879, gene |
neXtProti | NX_Q16656 |
OpenTargetsi | ENSG00000106459 |
PharmGKBi | PA31775 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | ENOG502QTK1, Eukaryota |
GeneTreei | ENSGT00390000006835 |
HOGENOMi | CLU_018156_3_1_1 |
InParanoidi | Q16656 |
OMAi | DHTVHQT |
PhylomeDBi | Q16656 |
TreeFami | TF105308 |
Enzyme and pathway databases
PathwayCommonsi | Q16656 |
Reactomei | R-HSA-1989781, PPARA activates gene expression R-HSA-2151201, Transcriptional activation of mitochondrial biogenesis |
SignaLinki | Q16656 |
SIGNORi | Q16656 |
Miscellaneous databases
BioGRID-ORCSi | 4899, 755 hits in 872 CRISPR screens |
ChiTaRSi | NRF1, human |
GeneWikii | NRF1 |
GenomeRNAii | 4899 |
Pharosi | Q16656, Tbio |
PROi | PR:Q16656 |
RNActi | Q16656, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000106459, Expressed in corpus callosum and 194 other tissues |
ExpressionAtlasi | Q16656, baseline and differential |
Genevisiblei | Q16656, HS |
Family and domain databases
InterProi | View protein in InterPro IPR039142, NRF1/Ewg IPR019526, Nrf1_activation-bd IPR019525, Nrf1_NLS/DNA-bd_dimer |
PANTHERi | PTHR20338, PTHR20338, 1 hit |
Pfami | View protein in Pfam PF10492, Nrf1_activ_bdg, 1 hit PF10491, Nrf1_DNA-bind, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | NRF1_HUMAN | |
Accessioni | Q16656Primary (citable) accession number: Q16656 Secondary accession number(s): A8K4C6 Q96AN2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 1, 2000 |
Last sequence update: | November 1, 1996 | |
Last modified: | December 2, 2020 | |
This is version 176 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- Human chromosome 7
Human chromosome 7: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families