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Protein

Prostasin

Gene

PRSS8

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Possesses a trypsin-like cleavage specificity with a preference for poly-basic substrates. Stimulates epithelial sodium channel (ENaC) activity through activating cleavage of the gamma subunits (SCNN1G).2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei85Charge relay system1
Active sitei134Charge relay system1
Active sitei238Charge relay system1

GO - Molecular functioni

  • endopeptidase activity Source: Reactome
  • serine-type endopeptidase activity Source: InterPro
  • serine-type peptidase activity Source: ProtInc
  • sodium channel regulator activity Source: GO_Central

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Protease, Serine protease

Enzyme and pathway databases

BRENDAi3.4.21.B6 2681
ReactomeiR-HSA-6809371 Formation of the cornified envelope

Protein family/group databases

MEROPSiS01.159

Names & Taxonomyi

Protein namesi
Recommended name:
Prostasin (EC:3.4.21.-)
Alternative name(s):
Channel-activating protease 1
Short name:
CAP1
Serine protease 8
Cleaved into the following 2 chains:
Gene namesi
Name:PRSS8
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 16

Organism-specific databases

EuPathDBiHostDB:ENSG00000052344.15
HGNCiHGNC:9491 PRSS8
MIMi600823 gene
neXtProtiNX_Q16651

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei320 – 340HelicalSequence analysisAdd BLAST21

Keywords - Cellular componenti

Cell membrane, Membrane, Secreted

Pathology & Biotechi

Organism-specific databases

DisGeNETi5652
OpenTargetsiENSG00000052344
PharmGKBiPA33840

Chemistry databases

ChEMBLiCHEMBL5610
DrugBankiDB06900 1-[4-(hydroxymethyl)phenyl]guanidine
GuidetoPHARMACOLOGYi2400

Polymorphism and mutation databases

BioMutaiPRSS8
DMDMi2833277

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 29Sequence analysisAdd BLAST29
PropeptideiPRO_000002802730 – 32Activation peptide3
ChainiPRO_000024051133 – 343ProstasinAdd BLAST311
ChainiPRO_000002802833 – 44Prostasin light chainAdd BLAST12
ChainiPRO_000002802945 – 322Prostasin heavy chainAdd BLAST278
PropeptideiPRO_0000028030323 – 343Add BLAST21

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi37 ↔ 154Interchain (between light and heavy chains)PROSITE-ProRule annotation
Disulfide bondi70 ↔ 86PROSITE-ProRule annotation1 Publication
Glycosylationi159N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi168 ↔ 244PROSITE-ProRule annotation1 Publication
Disulfide bondi201 ↔ 223PROSITE-ProRule annotation1 Publication
Disulfide bondi234 ↔ 262PROSITE-ProRule annotation1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

EPDiQ16651
MaxQBiQ16651
PaxDbiQ16651
PeptideAtlasiQ16651
PRIDEiQ16651
ProteomicsDBi61002

Expressioni

Tissue specificityi

Found in prostate, liver, salivary gland, kidney, lung, pancreas, colon, bronchus and renal proximal tubular cells. In the prostate gland it may be synthesized in epithelial cells, secreted into the ducts, and excreted into the seminal fluid.

Gene expression databases

BgeeiENSG00000052344 Expressed in 145 organ(s), highest expression level in body of stomach
CleanExiHS_PRSS8
ExpressionAtlasiQ16651 baseline and differential
GenevisibleiQ16651 HS

Organism-specific databases

HPAiHPA030436
HPA063533

Interactioni

Subunit structurei

Heterodimer of two chains, light and heavy, held by a disulfide bond.1 Publication

Protein-protein interaction databases

IntActiQ16651, 2 interactors
MINTiQ16651
STRINGi9606.ENSP00000319730

Chemistry databases

BindingDBiQ16651

Structurei

Secondary structure

1343
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ16651
SMRiQ16651
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ16651

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini45 – 286Peptidase S1PROSITE-ProRule annotationAdd BLAST242

Sequence similaritiesi

Belongs to the peptidase S1 family.PROSITE-ProRule annotation

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3627 Eukaryota
COG5640 LUCA
GeneTreeiENSGT00900000140825
HOGENOMiHOG000251820
HOVERGENiHBG013304
InParanoidiQ16651
KOiK08664
OMAiSYASWIH
OrthoDBiEOG091G0AH5
PhylomeDBiQ16651
TreeFamiTF351676

Family and domain databases

CDDicd00190 Tryp_SPc, 1 hit
InterProiView protein in InterPro
IPR009003 Peptidase_S1_PA
IPR001314 Peptidase_S1A
IPR001254 Trypsin_dom
IPR018114 TRYPSIN_HIS
IPR033116 TRYPSIN_SER
PfamiView protein in Pfam
PF00089 Trypsin, 1 hit
PRINTSiPR00722 CHYMOTRYPSIN
SMARTiView protein in SMART
SM00020 Tryp_SPc, 1 hit
SUPFAMiSSF50494 SSF50494, 1 hit
PROSITEiView protein in PROSITE
PS50240 TRYPSIN_DOM, 1 hit
PS00134 TRYPSIN_HIS, 1 hit
PS00135 TRYPSIN_SER, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q16651-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MAQKGVLGPG QLGAVAILLY LGLLRSGTGA EGAEAPCGVA PQARITGGSS
60 70 80 90 100
AVAGQWPWQV SITYEGVHVC GGSLVSEQWV LSAAHCFPSE HHKEAYEVKL
110 120 130 140 150
GAHQLDSYSE DAKVSTLKDI IPHPSYLQEG SQGDIALLQL SRPITFSRYI
160 170 180 190 200
RPICLPAANA SFPNGLHCTV TGWGHVAPSV SLLTPKPLQQ LEVPLISRET
210 220 230 240 250
CNCLYNIDAK PEEPHFVQED MVCAGYVEGG KDACQGDSGG PLSCPVEGLW
260 270 280 290 300
YLTGIVSWGD ACGARNRPGV YTLASSYASW IQSKVTELQP RVVPQTQESQ
310 320 330 340
PDSNLCGSHL AFSSAPAQGL LRPILFLPLG LALGLLSPWL SEH
Length:343
Mass (Da):36,431
Last modified:November 1, 1996 - v1
Checksum:i98DD6447F5A8C1B2
GO
Isoform 2 (identifier: Q16651-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     60-113: Missing.

Note: No experimental confirmation available.
Show »
Length:289
Mass (Da):30,519
Checksum:i4A85CFA9DE10BCDD
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
H3BUJ8H3BUJ8_HUMAN
Prostasin
PRSS8
195Annotation score:
H3BVC8H3BVC8_HUMAN
Prostasin
PRSS8
107Annotation score:

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_05663260 – 113Missing in isoform 2. 1 PublicationAdd BLAST54

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L41351 mRNA Translation: AAC41759.1
U33446 Genomic DNA Translation: AAB19071.1
AK301619 mRNA Translation: BAG63104.1
AC009088 Genomic DNA No translation available.
BC001462 mRNA Translation: AAH01462.1
CCDSiCCDS45469.1 [Q16651-1]
PIRiA57014
RefSeqiNP_002764.1, NM_002773.4 [Q16651-1]
UniGeneiHs.75799

Genome annotation databases

EnsembliENST00000317508; ENSP00000319730; ENSG00000052344 [Q16651-1]
ENST00000568261; ENSP00000457750; ENSG00000052344 [Q16651-2]
GeneIDi5652
KEGGihsa:5652
UCSCiuc002ebc.5 human [Q16651-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Web resourcesi

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L41351 mRNA Translation: AAC41759.1
U33446 Genomic DNA Translation: AAB19071.1
AK301619 mRNA Translation: BAG63104.1
AC009088 Genomic DNA No translation available.
BC001462 mRNA Translation: AAH01462.1
CCDSiCCDS45469.1 [Q16651-1]
PIRiA57014
RefSeqiNP_002764.1, NM_002773.4 [Q16651-1]
UniGeneiHs.75799

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3DFJX-ray1.45A45-289[»]
3DFLX-ray2.00A45-289[»]
3E0NX-ray1.70B45-305[»]
3E0PX-ray1.70B45-305[»]
3E16X-ray1.60B45-305[»]
3E1XX-ray1.70B45-305[»]
3FVFX-ray1.60B45-305[»]
3GYLX-ray1.30B45-305[»]
3GYMX-ray2.80A/B45-305[»]
ProteinModelPortaliQ16651
SMRiQ16651
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ16651, 2 interactors
MINTiQ16651
STRINGi9606.ENSP00000319730

Chemistry databases

BindingDBiQ16651
ChEMBLiCHEMBL5610
DrugBankiDB06900 1-[4-(hydroxymethyl)phenyl]guanidine
GuidetoPHARMACOLOGYi2400

Protein family/group databases

MEROPSiS01.159

Polymorphism and mutation databases

BioMutaiPRSS8
DMDMi2833277

Proteomic databases

EPDiQ16651
MaxQBiQ16651
PaxDbiQ16651
PeptideAtlasiQ16651
PRIDEiQ16651
ProteomicsDBi61002

Protocols and materials databases

DNASUi5652
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000317508; ENSP00000319730; ENSG00000052344 [Q16651-1]
ENST00000568261; ENSP00000457750; ENSG00000052344 [Q16651-2]
GeneIDi5652
KEGGihsa:5652
UCSCiuc002ebc.5 human [Q16651-1]

Organism-specific databases

CTDi5652
DisGeNETi5652
EuPathDBiHostDB:ENSG00000052344.15
GeneCardsiPRSS8
HGNCiHGNC:9491 PRSS8
HPAiHPA030436
HPA063533
MIMi600823 gene
neXtProtiNX_Q16651
OpenTargetsiENSG00000052344
PharmGKBiPA33840
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3627 Eukaryota
COG5640 LUCA
GeneTreeiENSGT00900000140825
HOGENOMiHOG000251820
HOVERGENiHBG013304
InParanoidiQ16651
KOiK08664
OMAiSYASWIH
OrthoDBiEOG091G0AH5
PhylomeDBiQ16651
TreeFamiTF351676

Enzyme and pathway databases

BRENDAi3.4.21.B6 2681
ReactomeiR-HSA-6809371 Formation of the cornified envelope

Miscellaneous databases

ChiTaRSiPRSS8 human
EvolutionaryTraceiQ16651
GeneWikiiPRSS8
GenomeRNAii5652
PROiPR:Q16651
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000052344 Expressed in 145 organ(s), highest expression level in body of stomach
CleanExiHS_PRSS8
ExpressionAtlasiQ16651 baseline and differential
GenevisibleiQ16651 HS

Family and domain databases

CDDicd00190 Tryp_SPc, 1 hit
InterProiView protein in InterPro
IPR009003 Peptidase_S1_PA
IPR001314 Peptidase_S1A
IPR001254 Trypsin_dom
IPR018114 TRYPSIN_HIS
IPR033116 TRYPSIN_SER
PfamiView protein in Pfam
PF00089 Trypsin, 1 hit
PRINTSiPR00722 CHYMOTRYPSIN
SMARTiView protein in SMART
SM00020 Tryp_SPc, 1 hit
SUPFAMiSSF50494 SSF50494, 1 hit
PROSITEiView protein in PROSITE
PS50240 TRYPSIN_DOM, 1 hit
PS00134 TRYPSIN_HIS, 1 hit
PS00135 TRYPSIN_SER, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiPRSS8_HUMAN
AccessioniPrimary (citable) accession number: Q16651
Secondary accession number(s): B4DWP2, Q9UCA3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: September 12, 2018
This is version 159 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human chromosome 16
    Human chromosome 16: entries, gene names and cross-references to MIM
  3. Peptidase families
    Classification of peptidase families and list of entries
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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Main funding by: National Institutes of Health

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