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Protein

POU domain class 2-associating factor 1

Gene

POU2AF1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Transcriptional coactivator that specifically associates with either OCT1 or OCT2. It boosts the OCT1 mediated promoter activity and to a lesser extent, that of OCT2. It has no intrinsic DNA-binding activity. It recognizes the POU domains of OCT1 and OCT2. It is essential for the response of B-cells to antigens and required for the formation of germinal centers.

GO - Molecular functioni

  • RNA polymerase II core promoter sequence-specific DNA binding Source: CAFA
  • RNA polymerase II proximal promoter sequence-specific DNA binding Source: CAFA
  • transcription coactivator activity Source: CAFA
  • transcription coregulator activity Source: ProtInc

GO - Biological processi

  • humoral immune response Source: ProtInc
  • positive regulation of transcription by RNA polymerase II Source: CAFA
  • transcription by RNA polymerase II Source: ProtInc

Keywordsi

Biological processTranscription, Transcription regulation

Enzyme and pathway databases

SIGNORiQ16633

Names & Taxonomyi

Protein namesi
Recommended name:
POU domain class 2-associating factor 1
Alternative name(s):
B-cell-specific coactivator OBF-1
BOB-1
OCA-B
OCT-binding factor 1
Gene namesi
Name:POU2AF1
Synonyms:OBF1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 11

Organism-specific databases

EuPathDBiHostDB:ENSG00000110777.11
HGNCiHGNC:9211 POU2AF1
MIMi601206 gene
neXtProtiNX_Q16633

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Involvement in diseasei

A chromosomal aberration involving POU2AF1/OBF1 may be a cause of a form of B-cell leukemia. Translocation t(3;11)(q27;q23) with BCL6.1 Publication

Keywords - Diseasei

Proto-oncogene

Organism-specific databases

DisGeNETi5450
MalaCardsiPOU2AF1
OpenTargetsiENSG00000110777
Orphaneti186 Primary biliary cholangitis
PharmGKBiPA33535

Polymorphism and mutation databases

BioMutaiPOU2AF1
DMDMi2833276

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000580181 – 256POU domain class 2-associating factor 1Add BLAST256

Post-translational modificationi

Ubiquitinated; mediated by SIAH1 or SIAH2 and leading to its subsequent proteasomal degradation.Curated

Keywords - PTMi

Ubl conjugation

Proteomic databases

MaxQBiQ16633
PaxDbiQ16633
PeptideAtlasiQ16633
PRIDEiQ16633
ProteomicsDBi60982

PTM databases

iPTMnetiQ16633
PhosphoSitePlusiQ16633

Expressioni

Tissue specificityi

B-cell specific.

Gene expression databases

BgeeiENSG00000110777 Expressed in 160 organ(s), highest expression level in vermiform appendix
CleanExiHS_POU2AF1
ExpressionAtlasiQ16633 baseline and differential
GenevisibleiQ16633 HS

Organism-specific databases

HPAiCAB011193

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
SIAH1Q8IUQ42EBI-943588,EBI-747107

Protein-protein interaction databases

BioGridi111446, 14 interactors
CORUMiQ16633
ELMiQ16633
IntActiQ16633, 41 interactors
STRINGi9606.ENSP00000376786

Structurei

Secondary structure

1256
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

DisProtiDP00172
ProteinModelPortaliQ16633
SMRiQ16633
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ16633

Family & Domainsi

Sequence similaritiesi

Belongs to the POU2AF1 family.Curated

Phylogenomic databases

eggNOGiENOG410IJXZ Eukaryota
ENOG41123CN LUCA
GeneTreeiENSGT00390000017499
HOGENOMiHOG000059584
HOVERGENiHBG007859
InParanoidiQ16633
OMAiDPRRAMN
OrthoDBiEOG091G0EUL
PhylomeDBiQ16633
TreeFamiTF332565

Family and domain databases

InterProiView protein in InterPro
IPR015389 PD-C2-AF1
PfamiView protein in Pfam
PF09310 PD-C2-AF1, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

Q16633-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MLWQKPTAPE QAPAPARPYQ GVRVKEPVKE LLRRKRGHAS SGAAPAPTAV
60 70 80 90 100
VLPHQPLATY TTVGPSCLDM EGSVSAVTEE AALCAGWLSQ PTPATLQPLA
110 120 130 140 150
PWTPYTEYVP HEAVSCPYSA DMYVQPVCPS YTVVGPSSVL TYASPPLITN
160 170 180 190 200
VTTRSSATPA VGPPLEGPEH QAPLTYFPWP QPLSTLPTST LQYQPPAPAL
210 220 230 240 250
PGPQFVQLPI SIPEPVLQDM EDPRRAASSL TIDKLLLEEE DSDAYALNHT

LSVEGF
Length:256
Mass (Da):27,436
Last modified:November 1, 1996 - v1
Checksum:i2C46F1796774D614
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
E9PKH4E9PKH4_HUMAN
POU domain class 2-associating fact...
POU2AF1
127Annotation score:

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_005521141T → A. Corresponds to variant dbSNP:rs1042750Ensembl.1
Natural variantiVAR_005522194Q → R. Corresponds to variant dbSNP:rs1042751Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z47550 mRNA Translation: CAA87630.1
X83504 mRNA Translation: CAA58494.1
Z49194 mRNA Translation: CAA89053.1
AK313573 mRNA Translation: BAG36346.1
CH471065 Genomic DNA Translation: EAW67137.1
BC032549 mRNA Translation: AAH32549.1
CCDSiCCDS31675.1
PIRiA55652
RefSeqiNP_006226.2, NM_006235.2
UniGeneiHs.654525

Genome annotation databases

EnsembliENST00000393067; ENSP00000376786; ENSG00000110777
GeneIDi5450
KEGGihsa:5450
UCSCiuc001plg.5 human

Keywords - Coding sequence diversityi

Chromosomal rearrangement, Polymorphism

Similar proteinsi

Cross-referencesi

Web resourcesi

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z47550 mRNA Translation: CAA87630.1
X83504 mRNA Translation: CAA58494.1
Z49194 mRNA Translation: CAA89053.1
AK313573 mRNA Translation: BAG36346.1
CH471065 Genomic DNA Translation: EAW67137.1
BC032549 mRNA Translation: AAH32549.1
CCDSiCCDS31675.1
PIRiA55652
RefSeqiNP_006226.2, NM_006235.2
UniGeneiHs.654525

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1CQTX-ray3.20I/J1-44[»]
DisProtiDP00172
ProteinModelPortaliQ16633
SMRiQ16633
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi111446, 14 interactors
CORUMiQ16633
ELMiQ16633
IntActiQ16633, 41 interactors
STRINGi9606.ENSP00000376786

PTM databases

iPTMnetiQ16633
PhosphoSitePlusiQ16633

Polymorphism and mutation databases

BioMutaiPOU2AF1
DMDMi2833276

Proteomic databases

MaxQBiQ16633
PaxDbiQ16633
PeptideAtlasiQ16633
PRIDEiQ16633
ProteomicsDBi60982

Protocols and materials databases

DNASUi5450
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000393067; ENSP00000376786; ENSG00000110777
GeneIDi5450
KEGGihsa:5450
UCSCiuc001plg.5 human

Organism-specific databases

CTDi5450
DisGeNETi5450
EuPathDBiHostDB:ENSG00000110777.11
GeneCardsiPOU2AF1
HGNCiHGNC:9211 POU2AF1
HPAiCAB011193
MalaCardsiPOU2AF1
MIMi601206 gene
neXtProtiNX_Q16633
OpenTargetsiENSG00000110777
Orphaneti186 Primary biliary cholangitis
PharmGKBiPA33535
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IJXZ Eukaryota
ENOG41123CN LUCA
GeneTreeiENSGT00390000017499
HOGENOMiHOG000059584
HOVERGENiHBG007859
InParanoidiQ16633
OMAiDPRRAMN
OrthoDBiEOG091G0EUL
PhylomeDBiQ16633
TreeFamiTF332565

Enzyme and pathway databases

SIGNORiQ16633

Miscellaneous databases

ChiTaRSiPOU2AF1 human
EvolutionaryTraceiQ16633
GeneWikiiPOU2AF1
GenomeRNAii5450
PROiPR:Q16633
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000110777 Expressed in 160 organ(s), highest expression level in vermiform appendix
CleanExiHS_POU2AF1
ExpressionAtlasiQ16633 baseline and differential
GenevisibleiQ16633 HS

Family and domain databases

InterProiView protein in InterPro
IPR015389 PD-C2-AF1
PfamiView protein in Pfam
PF09310 PD-C2-AF1, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiOBF1_HUMAN
AccessioniPrimary (citable) accession number: Q16633
Secondary accession number(s): B2R8Z9, Q14983
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: November 7, 2018
This is version 161 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. Human chromosome 11
    Human chromosome 11: entries, gene names and cross-references to MIM
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