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Protein

Transcription initiation factor TFIID subunit 9

Gene

TAF9

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Essential for cell viability. TAF9 and TAF9B are involved in transcriptional activation as well as repression of distinct but overlapping sets of genes. May have a role in gene regulation associated with apoptosis. TAFs are components of the transcription factor IID (TFIID) complex, the TBP-free TAFII complex (TFTC), the PCAF histone acetylase complex and the STAGA transcription coactivator-HAT complex. TFIID or TFTC are essential for the regulation of RNA polymerase II-mediated transcription.1 Publication

Caution

AK6 and TAF9 were initially considered as products of the same gene since they share two exons. However, they are translated from different initiation codons and reading frames and encode unrelated proteins. This arrangement is conserved in some mammalian species.Curated

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi120 – 137Add BLAST18

GO - Molecular functioni

  • activating transcription factor binding Source: BHF-UCL
  • ATPase binding Source: UniProtKB
  • C2H2 zinc finger domain binding Source: BHF-UCL
  • DNA binding Source: UniProtKB
  • p53 binding Source: BHF-UCL
  • protein heterodimerization activity Source: InterPro
  • transcription coactivator activity Source: UniProtKB
  • transcription regulatory region DNA binding Source: BHF-UCL

GO - Biological processi

  • box C/D snoRNP assembly Source: UniProtKB
  • cellular response to DNA damage stimulus Source: BHF-UCL
  • histone H3 acetylation Source: UniProtKB
  • negative regulation of apoptotic process Source: BHF-UCL
  • negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator Source: BHF-UCL
  • negative regulation of proteasomal ubiquitin-dependent protein catabolic process Source: BHF-UCL
  • positive regulation of cell growth Source: UniProtKB
  • positive regulation of response to cytokine stimulus Source: BHF-UCL
  • positive regulation of transcription by RNA polymerase II Source: BHF-UCL
  • protein stabilization Source: BHF-UCL
  • regulation of signal transduction by p53 class mediator Source: Reactome
  • response to interleukin-1 Source: BHF-UCL
  • RNA polymerase II transcriptional preinitiation complex assembly Source: GOC
  • snRNA transcription by RNA polymerase II Source: Reactome
  • transcription by RNA polymerase II Source: Reactome
  • transcription initiation from RNA polymerase II promoter Source: Reactome

Keywordsi

Molecular functionDNA-binding
Biological processTranscription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-HSA-167161 HIV Transcription Initiation
R-HSA-167162 RNA Polymerase II HIV Promoter Escape
R-HSA-167172 Transcription of the HIV genome
R-HSA-3214847 HATs acetylate histones
R-HSA-674695 RNA Polymerase II Pre-transcription Events
R-HSA-6804756 Regulation of TP53 Activity through Phosphorylation
R-HSA-6807505 RNA polymerase II transcribes snRNA genes
R-HSA-73776 RNA Polymerase II Promoter Escape
R-HSA-73779 RNA Polymerase II Transcription Pre-Initiation And Promoter Opening
R-HSA-75953 RNA Polymerase II Transcription Initiation
R-HSA-76042 RNA Polymerase II Transcription Initiation And Promoter Clearance

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription initiation factor TFIID subunit 9
Alternative name(s):
RNA polymerase II TBP-associated factor subunit G
STAF31/32
Transcription initiation factor TFIID 31 kDa subunit
Short name:
TAFII-31
Short name:
TAFII31
Transcription initiation factor TFIID 32 kDa subunit
Short name:
TAFII-32
Short name:
TAFII32
Gene namesi
Name:TAF9
Synonyms:TAF2G, TAFII31
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 5

Organism-specific databases

EuPathDBiHostDB:ENSG00000273841.4
HGNCiHGNC:11542 TAF9
MIMi600822 gene
neXtProtiNX_Q16594

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi6880
OpenTargetsiENSG00000273841
PharmGKBiPA36317

Polymorphism and mutation databases

BioMutaiSLC26A3
DMDMi2498981

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001188881 – 264Transcription initiation factor TFIID subunit 9Add BLAST264

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei5N6-acetyllysineCombined sources1
Modified residuei149PhosphoserineCombined sources1
Modified residuei152PhosphoserineCombined sources1
Modified residuei155PhosphoserineBy similarity1
Modified residuei158PhosphoserineCombined sources1
Modified residuei159PhosphothreonineCombined sources1
Modified residuei161PhosphothreonineCombined sources1
Modified residuei164PhosphothreonineBy similarity1
Modified residuei178PhosphothreonineCombined sources1
Modified residuei181PhosphoserineCombined sources1
Modified residuei196PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ16594
MaxQBiQ16594
PaxDbiQ16594
PeptideAtlasiQ16594
PRIDEiQ16594
ProteomicsDBi60938

PTM databases

iPTMnetiQ16594
PhosphoSitePlusiQ16594

Expressioni

Inductioni

6 to 8-fold by apoptotic signals.1 Publication

Gene expression databases

BgeeiENSG00000273841
CleanExiHS_TAF9
ExpressionAtlasiQ16594 baseline and differential
GenevisibleiQ16594 HS

Interactioni

Subunit structurei

Component of TFIID, the TATA-binding protein-free TAF complex (TFTC), the PCAF complex and the STAGA transcription coactivator-HAT complex. The PCAF complex consists at least of TADA2L/ADA2, SUPT3H/SPT3, TADA3L/ADA3, TAF5L/PAF65-beta, TAF6L/PAF65-alpha, TAF10/TAFII30, TAF12/TAFII20, TAF9/TAFII31 and TRRAP. The STAGA transcription coactivator-HAT complex consists at least of SUPT3H, GCN5L2, SUPT7L, TAF5L, TAF6L, TADA3L, TAD1L, TAF10, TAF12, TRRAP and TAF9. Binds N-terminal domain of p53/TP53 which is essential for transcription. Component of some MLL1/MLL complex, at least composed of the core components KMT2A/MLL1, ASH2L, HCFC1/HCF1, WDR5 and RBBP5, as well as the facultative components BAP18, CHD8, E2F6, HSP70, INO80C, KANSL1, LAS1L, MAX, MCRS1, MGA, MYST1/MOF, PELP1, PHF20, PRP31, RING2, RUVB1/TIP49A, RUVB2/TIP49B, SENP3, TAF1, TAF4, TAF6, TAF7, TAF9 and TEX10. Binds TFIIB and the Herpes simplex virus activator VP16. Forms a heterodimer with TAF6/TAFII80 in a complex with the TAF4B/TAFII105-TAF12/TAFII20 heterodimer. Also interacts with TAF5. Binds directly DNA. Increased DNA binding when complexed with TAF6/TAFII80.8 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

  • activating transcription factor binding Source: BHF-UCL
  • ATPase binding Source: UniProtKB
  • C2H2 zinc finger domain binding Source: BHF-UCL
  • p53 binding Source: BHF-UCL
  • protein heterodimerization activity Source: InterPro

Protein-protein interaction databases

BioGridi112743, 91 interactors
ComplexPortaliCPX-900 SAGA complex
CPX-903 TFTC histone acetylation complex
CPX-915 General transcription factor complex TFIID
CPX-930 General transcription factor complex TFIID, TAF4B variant
CPX-989 PCAF histone acetylase complex
CORUMiQ16594
DIPiDIP-435N
IntActiQ16594, 55 interactors
MINTiQ16594
STRINGi9606.ENSP00000217893

Structurei

3D structure databases

ProteinModelPortaliQ16594
SMRiQ16594
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi250 – 262Poly-AspAdd BLAST13

Sequence similaritiesi

Belongs to the TAF9 family.Curated

Phylogenomic databases

eggNOGiKOG3334 Eukaryota
COG5094 LUCA
GeneTreeiENSGT00390000001626
HOGENOMiHOG000231730
HOVERGENiHBG002304
InParanoidiQ16594
KOiK14535
OMAiIQCRMDQ
OrthoDBiEOG091G0RPE
PhylomeDBiQ16594
TreeFamiTF351417

Family and domain databases

CDDicd07979 TAF9, 1 hit
Gene3Di1.10.20.10, 1 hit
InterProiView protein in InterPro
IPR009072 Histone-fold
IPR003162 TFIID-31
PfamiView protein in Pfam
PF02291 TFIID-31kDa, 1 hit
SUPFAMiSSF47113 SSF47113, 1 hit

Sequencei

Sequence statusi: Complete.

Q16594-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MESGKTASPK SMPKDAQMMA QILKDMGITE YEPRVINQML EFAFRYVTTI
60 70 80 90 100
LDDAKIYSSH AKKATVDADD VRLAIQCRAD QSFTSPPPRD FLLDIARQRN
110 120 130 140 150
QTPLPLIKPY SGPRLPPDRY CLTAPNYRLK SLQKKASTSA GRITVPRLSV
160 170 180 190 200
GSVTSRPSTP TLGTPTPQTM SVSTKVGTPM SLTGQRFTVQ MPTSQSPAVK
210 220 230 240 250
ASIPATSAVQ NVLINPSLIG SKNILITTNM MSSQNTANES SNALKRKRED
260
DDDDDDDDDD YDNL
Length:264
Mass (Da):28,974
Last modified:November 1, 1997 - v1
Checksum:i1925AEC65D6C84C7
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti46Y → V AA sequence (PubMed:9674425).Curated1
Sequence conflicti225L → F in AAH03400 (PubMed:15489334).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_0162796T → M1 PublicationCorresponds to variant dbSNP:rs4252233Ensembl.1
Natural variantiVAR_052260210Q → H. Corresponds to variant dbSNP:rs11542580Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U25112 mRNA Translation: AAA91318.1
U21858 mRNA Translation: AAC50153.1
U30504 mRNA Translation: AAA84389.1
BT019652 mRNA Translation: AAV38458.1
AY189986 Genomic DNA Translation: AAN84793.1
CH471137 Genomic DNA Translation: EAW51295.1
CH471137 Genomic DNA Translation: EAW51296.1
BC003400 mRNA Translation: AAH03400.1
BC033320 mRNA Translation: AAH33320.1
CCDSiCCDS4002.1
PIRiI39141
RefSeqiNP_001015892.1, NM_001015892.1
NP_003178.1, NM_003187.4
UniGeneiHs.653163

Genome annotation databases

EnsembliENST00000217893; ENSP00000217893; ENSG00000273841
ENST00000328663; ENSP00000370193; ENSG00000273841
ENST00000506736; ENSP00000421873; ENSG00000273841
ENST00000615404; ENSP00000478935; ENSG00000276463
ENST00000616867; ENSP00000477750; ENSG00000276463
ENST00000617893; ENSP00000477611; ENSG00000276463
GeneIDi6880
KEGGihsa:6880
UCSCiuc003jwc.2 human

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Entry informationi

Entry nameiTAF9_HUMAN
AccessioniPrimary (citable) accession number: Q16594
Secondary accession number(s): D3DWA3, Q5U0D1, Q9BTS1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: June 20, 2018
This is version 184 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 5
    Human chromosome 5: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

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