UniProtKB - Q16514 (TAF12_HUMAN)
Transcription initiation factor TFIID subunit 12
TAF12
Functioni
The TFIID basal transcription factor complex plays a major role in the initiation of RNA polymerase II (Pol II)-dependent transcription (PubMed:33795473).
TFIID recognizes and binds promoters with or without a TATA box via its subunit TBP, a TATA-box-binding protein, and promotes assembly of the pre-initiation complex (PIC) (PubMed:33795473).
The TFIID complex consists of TBP and TBP-associated factors (TAFs), including TAF1, TAF2, TAF3, TAF4, TAF5, TAF6, TAF7, TAF8, TAF9, TAF10, TAF11, TAF12 and TAF13 (PubMed:33795473).
Component of the TATA-binding protein-free TAF complex (TFTC), the PCAF histone acetylase complex and the STAGA transcription coactivator-HAT complex (PubMed:10373431, PubMed:7729427, PubMed:8598932, PubMed:8663456, PubMed:9674425, PubMed:9885574).
7 PublicationsGO - Molecular functioni
- DNA binding Source: UniProtKB
- DNA-binding transcription factor binding Source: UniProtKB
- protein heterodimerization activity Source: InterPro
- TBP-class protein binding Source: GO_Central
- transcription coactivator activity Source: UniProtKB
GO - Biological processi
- DNA-templated transcription, initiation Source: UniProtKB
- histone acetylation Source: ComplexPortal
- histone H3 acetylation Source: UniProtKB
- monoubiquitinated histone deubiquitination Source: ComplexPortal
- monoubiquitinated histone H2A deubiquitination Source: ComplexPortal
- mRNA transcription by RNA polymerase II Source: ComplexPortal
- positive regulation of DNA-binding transcription factor activity Source: UniProtKB
- positive regulation of transcription, DNA-templated Source: ComplexPortal
- positive regulation of transcription by RNA polymerase II Source: UniProtKB
- positive regulation of transcription initiation from RNA polymerase II promoter Source: ComplexPortal
- protein phosphorylation Source: ComplexPortal
- regulation of DNA repair Source: ComplexPortal
- regulation of RNA splicing Source: ComplexPortal
- regulation of transcription, DNA-templated Source: ComplexPortal
- regulation of transcription by RNA polymerase II Source: ComplexPortal
- RNA polymerase II preinitiation complex assembly Source: ComplexPortal
- transcription by RNA polymerase II Source: ProtInc
- transcription initiation from RNA polymerase II promoter Source: UniProtKB
Keywordsi
Biological process | Transcription, Transcription regulation |
Enzyme and pathway databases
PathwayCommonsi | Q16514 |
Reactomei | R-HSA-167161, HIV Transcription Initiation R-HSA-167162, RNA Polymerase II HIV Promoter Escape R-HSA-167172, Transcription of the HIV genome R-HSA-3214847, HATs acetylate histones R-HSA-674695, RNA Polymerase II Pre-transcription Events R-HSA-6804756, Regulation of TP53 Activity through Phosphorylation R-HSA-73776, RNA Polymerase II Promoter Escape R-HSA-73779, RNA Polymerase II Transcription Pre-Initiation And Promoter Opening R-HSA-75953, RNA Polymerase II Transcription Initiation R-HSA-76042, RNA Polymerase II Transcription Initiation And Promoter Clearance |
SignaLinki | Q16514 |
SIGNORi | Q16514 |
Names & Taxonomyi
Protein namesi | Recommended name: Transcription initiation factor TFIID subunit 12CuratedAlternative name(s): Transcription initiation factor TFIID 20/15 kDa subunitsCurated Short name: TAFII-20/TAFII-15Curated Short name: TAFII20/TAFII15Imported |
Gene namesi | |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:11545, TAF12 |
MIMi | 600773, gene |
neXtProti | NX_Q16514 |
VEuPathDBi | HostDB:ENSG00000120656 |
Subcellular locationi
Nucleus
- Nucleus 2 Publications
Nucleus
- nucleoplasm Source: Reactome
- nucleus Source: UniProtKB
- SAGA complex Source: UniProtKB
- SLIK (SAGA-like) complex Source: InterPro
- transcription factor TFIID complex Source: ARUK-UCL
- transcription factor TFTC complex Source: UniProtKB
Keywords - Cellular componenti
NucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 87 – 91 | IADDF → EADDK: Drastically reduces binding to TAF4. 1 Publication | 5 | |
Mutagenesisi | 95 – 96 | VV → EK: Drastically reduces binding to TAF4. 1 Publication | 2 | |
Mutagenesisi | 99 – 103 | ACQLA → RCQLR: Drastically reduces binding to TAF4. 1 Publication | 5 |
Organism-specific databases
DisGeNETi | 6883 |
OpenTargetsi | ENSG00000120656 |
PharmGKBi | PA36320 |
Miscellaneous databases
Pharosi | Q16514, Tbio |
Genetic variation databases
BioMutai | TAF12 |
DMDMi | 3024708 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000033580 | 1 – 161 | Transcription initiation factor TFIID subunit 12Add BLAST | 161 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Cross-linki | 19 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources | ||
Modified residuei | 43 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 51 | PhosphoserineCombined sources | 1 | |
Modified residuei | 59 | PhosphothreonineCombined sources | 1 |
Keywords - PTMi
Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
EPDi | Q16514 |
jPOSTi | Q16514 |
MassIVEi | Q16514 |
MaxQBi | Q16514 |
PaxDbi | Q16514 |
PeptideAtlasi | Q16514 |
PRIDEi | Q16514 |
ProteomicsDBi | 60885 [Q16514-1] 60886 [Q16514-2] |
PTM databases
iPTMneti | Q16514 |
PhosphoSitePlusi | Q16514 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSG00000120656, Expressed in oocyte and 222 other tissues |
Genevisiblei | Q16514, HS |
Organism-specific databases
HPAi | ENSG00000120656, Low tissue specificity |
Interactioni
Subunit structurei
Component of the TFIID basal transcription factor complex, composed of TATA-box-binding protein TBP, and a number of TBP-associated factors (TAFs), including TAF1, TAF2, TAF3, TAF4, TAF5, TAF6, TAF7, TAF8, TAF9, TAF10, TAF11, TAF12 and TAF13 (PubMed:33795473).
Component of the TATA-binding protein-free TAF complex (TFTC), the PCAF histone acetylase complex and the STAGA transcription coactivator-HAT complex (PubMed:10373431, PubMed:11564863, PubMed:9885574, PubMed:10594036).
Component of the PCAF complex, at least composed of TADA2L/ADA2, TADA3L/ADA3, TAF5L/PAF65-beta, SUPT3H, TAF6L, TAF9, TAF10, TAF12 and TRRAP (PubMed:9885574).
Component of the STAGA transcription coactivator-HAT complex, at least composed of SUPT3H, GCN5L2, TAF5L, TAF6L, STAF65-gamma/SUPT7L, TADA3L, TAD1L, TAF10, TAF12, TRRAP and TAF9 (PubMed:11564863, PubMed:10594036).
Interacts with ATF7 (via the transactivation domain); the interaction is prevented by sumoylation of ATF7 (PubMed:15735663).
6 PublicationsBinary interactionsi
Q16514
With | #Exp. | IntAct |
---|---|---|
PRKAR2B [P31323] | 3 | EBI-1034238,EBI-2930670 |
PRKAR2B [Q6DHZ2] | 3 | EBI-1034238,EBI-10185196 |
TAF1C [Q15572] | 2 | EBI-1034238,EBI-2510659 |
TBP [P20226] | 3 | EBI-1034238,EBI-355371 |
Isoform TAFII20 [Q16514-1]
With | #Exp. | IntAct |
---|---|---|
TAF4 [O00268] | 16 | EBI-1034253,EBI-1034261 |
GO - Molecular functioni
- DNA-binding transcription factor binding Source: UniProtKB
- protein heterodimerization activity Source: InterPro
- TBP-class protein binding Source: GO_Central
Protein-protein interaction databases
BioGRIDi | 112746, 83 interactors |
ComplexPortali | CPX-6802, SAGA complex, KAT2B variant CPX-900, SAGA complex, KAT2A variant CPX-903, TFTC histone acetylation complex CPX-915, General transcription factor complex TFIID CPX-930, General transcription factor complex TFIID, TAF4B variant CPX-989, PCAF histone acetylase complex |
CORUMi | Q16514 |
DIPi | DIP-496N |
IntActi | Q16514, 50 interactors |
MINTi | Q16514 |
STRINGi | 9606.ENSP00000263974 |
Miscellaneous databases
RNActi | Q16514, protein |
Structurei
Secondary structure
3D structure databases
AlphaFoldDBi | Q16514 |
SMRi | Q16514 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q16514 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 59 – 126 | Histone-foldSequence analysisAdd BLAST | 68 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 15 – 55 | DisorderedSequence analysisAdd BLAST | 41 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 15 – 37 | Polar residuesSequence analysisAdd BLAST | 23 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG1142, Eukaryota |
GeneTreei | ENSGT00390000002144 |
HOGENOMi | CLU_093619_3_1_1 |
InParanoidi | Q16514 |
OMAi | HRRDTTV |
OrthoDBi | 1569957at2759 |
PhylomeDBi | Q16514 |
TreeFami | TF323652 |
Family and domain databases
CDDi | cd07981, TAF12, 1 hit |
Gene3Di | 1.10.20.10, 1 hit |
IDEALi | IID00248 |
InterProi | View protein in InterPro IPR009072, Histone-fold IPR037794, TAF12 IPR003228, TFIID_TAF12_dom |
PANTHERi | PTHR12264, PTHR12264, 1 hit |
Pfami | View protein in Pfam PF03847, TFIID_20kDa, 1 hit |
SUPFAMi | SSF47113, SSF47113, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative initiation. AlignAdd to basketThis entry has 2 described isoforms and 10 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MNQFGPSALI NLSNFSSIKP EPASTPPQGS MANSTAVVKI PGTPGAGGRL
60 70 80 90 100
SPENNQVLTK KKLQDLVREV DPNEQLDEDV EEMLLQIADD FIESVVTAAC
110 120 130 140 150
QLARHRKSST LEVKDVQLHL ERQWNMWIPG FGSEEIRPYK KACTTEAHKQ
160
RMALIRKTTK K
The sequence of this isoform differs from the canonical sequence as follows:
1-30: Missing.
Computationally mapped potential isoform sequencesi
There are 10 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A804HLG9 | A0A804HLG9_HUMAN | Transcription initiation factor TFI... | TAF12 hCG_1747308 | 213 | Annotation score: | ||
A0A8I5KP17 | A0A8I5KP17_HUMAN | Transcription initiation factor TFI... | TAF12 | 79 | Annotation score: | ||
A0A8I5KPH6 | A0A8I5KPH6_HUMAN | Transcription initiation factor TFI... | TAF12 | 41 | Annotation score: | ||
A0A8I5KTB6 | A0A8I5KTB6_HUMAN | Transcription initiation factor TFI... | TAF12 | 228 | Annotation score: | ||
A0A8I5KU09 | A0A8I5KU09_HUMAN | Transcription initiation factor TFI... | TAF12 | 194 | Annotation score: | ||
A0A8I5KWX5 | A0A8I5KWX5_HUMAN | Transcription initiation factor TFI... | TAF12 | 112 | Annotation score: | ||
A0A8I5KXE7 | A0A8I5KXE7_HUMAN | Transcription initiation factor TFI... | TAF12 | 168 | Annotation score: | ||
A0A8I5KYJ9 | A0A8I5KYJ9_HUMAN | Transcription initiation factor TFI... | TAF12 | 136 | Annotation score: | ||
A0A8I5KYW6 | A0A8I5KYW6_HUMAN | Transcription initiation factor TFI... | TAF12 | 162 | Annotation score: | ||
A0A8I5QKX2 | A0A8I5QKX2_HUMAN | Transcription initiation factor TFI... | TAF12 | 189 | Annotation score: |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_018888 | 1 – 30 | Missing in isoform TAFII15. 1 PublicationAdd BLAST | 30 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U57693 mRNA Translation: AAC50600.1 U57693 mRNA Translation: AAC50601.1 D50544 mRNA Translation: BAA09112.1 X84002 mRNA Translation: CAA58826.1 BT007031 mRNA Translation: AAP35678.1 AY206865 Genomic DNA Translation: AAO13491.1 AL513497 Genomic DNA No translation available. CH471059 Genomic DNA Translation: EAX07682.1 CH471059 Genomic DNA Translation: EAX07683.1 BC011986 mRNA Translation: AAH11986.1 |
CCDSi | CCDS326.1 [Q16514-1] |
PIRi | JC4676 |
RefSeqi | NP_001128690.1, NM_001135218.1 [Q16514-1] NP_005635.1, NM_005644.3 [Q16514-1] |
Genome annotation databases
Ensembli | ENST00000263974.4; ENSP00000263974.4; ENSG00000120656.14 ENST00000373824.9; ENSP00000362930.4; ENSG00000120656.14 ENST00000685312.1; ENSP00000509153.1; ENSG00000120656.14 ENST00000689843.1; ENSP00000509370.1; ENSG00000120656.14 ENST00000692098.1; ENSP00000509051.1; ENSG00000120656.14 |
GeneIDi | 6883 |
KEGGi | hsa:6883 |
MANE-Selecti | ENST00000373824.9; ENSP00000362930.4; NM_005644.4; NP_005635.1 |
UCSCi | uc001bqx.5, human [Q16514-1] |
Keywords - Coding sequence diversityi
Alternative initiationSimilar proteinsi
Cross-referencesi
Web resourcesi
NIEHS-SNPs |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U57693 mRNA Translation: AAC50600.1 U57693 mRNA Translation: AAC50601.1 D50544 mRNA Translation: BAA09112.1 X84002 mRNA Translation: CAA58826.1 BT007031 mRNA Translation: AAP35678.1 AY206865 Genomic DNA Translation: AAO13491.1 AL513497 Genomic DNA No translation available. CH471059 Genomic DNA Translation: EAX07682.1 CH471059 Genomic DNA Translation: EAX07683.1 BC011986 mRNA Translation: AAH11986.1 |
CCDSi | CCDS326.1 [Q16514-1] |
PIRi | JC4676 |
RefSeqi | NP_001128690.1, NM_001135218.1 [Q16514-1] NP_005635.1, NM_005644.3 [Q16514-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1H3O | X-ray | 2.30 | B/D | 57-128 | [»] | |
6MZC | electron microscopy | 4.50 | R | 1-161 | [»] | |
6MZD | electron microscopy | 9.80 | Q | 1-161 | [»] | |
6MZL | electron microscopy | 23.00 | Q/R | 1-161 | [»] | |
6MZM | electron microscopy | 7.50 | R | 1-161 | [»] | |
7EDX | electron microscopy | 4.50 | L/l | 1-161 | [»] | |
7EG7 | electron microscopy | 6.20 | L/l | 1-161 | [»] | |
7EG8 | electron microscopy | 7.40 | L/l | 1-161 | [»] | |
7EG9 | electron microscopy | 3.70 | L/l | 1-161 | [»] | |
7EGA | electron microscopy | 4.10 | L/l | 1-161 | [»] | |
7EGB | electron microscopy | 3.30 | L/l | 1-161 | [»] | |
7EGC | electron microscopy | 3.90 | L/l | 1-161 | [»] | |
7EGD | electron microscopy | 6.75 | L/l | 1-161 | [»] | |
7EGE | electron microscopy | 9.00 | L/l | 1-161 | [»] | |
7EGF | electron microscopy | 3.16 | l | 1-161 | [»] | |
7EGG | electron microscopy | 2.77 | L | 1-161 | [»] | |
7EGI | electron microscopy | 9.82 | L/l | 1-161 | [»] | |
7EGJ | electron microscopy | 8.64 | L/l | 1-161 | [»] | |
7ENA | electron microscopy | 4.07 | DL/Dl | 1-161 | [»] | |
7ENC | electron microscopy | 4.13 | DL/Dl | 1-161 | [»] | |
7KTR | electron microscopy | 2.93 | G | 1-161 | [»] | |
7KTS | electron microscopy | 19.09 | G | 1-161 | [»] | |
AlphaFoldDBi | Q16514 | |||||
SMRi | Q16514 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 112746, 83 interactors |
ComplexPortali | CPX-6802, SAGA complex, KAT2B variant CPX-900, SAGA complex, KAT2A variant CPX-903, TFTC histone acetylation complex CPX-915, General transcription factor complex TFIID CPX-930, General transcription factor complex TFIID, TAF4B variant CPX-989, PCAF histone acetylase complex |
CORUMi | Q16514 |
DIPi | DIP-496N |
IntActi | Q16514, 50 interactors |
MINTi | Q16514 |
STRINGi | 9606.ENSP00000263974 |
PTM databases
iPTMneti | Q16514 |
PhosphoSitePlusi | Q16514 |
Genetic variation databases
BioMutai | TAF12 |
DMDMi | 3024708 |
Proteomic databases
EPDi | Q16514 |
jPOSTi | Q16514 |
MassIVEi | Q16514 |
MaxQBi | Q16514 |
PaxDbi | Q16514 |
PeptideAtlasi | Q16514 |
PRIDEi | Q16514 |
ProteomicsDBi | 60885 [Q16514-1] 60886 [Q16514-2] |
Protocols and materials databases
Antibodypediai | 1777, 212 antibodies from 27 providers |
DNASUi | 6883 |
Genome annotation databases
Ensembli | ENST00000263974.4; ENSP00000263974.4; ENSG00000120656.14 ENST00000373824.9; ENSP00000362930.4; ENSG00000120656.14 ENST00000685312.1; ENSP00000509153.1; ENSG00000120656.14 ENST00000689843.1; ENSP00000509370.1; ENSG00000120656.14 ENST00000692098.1; ENSP00000509051.1; ENSG00000120656.14 |
GeneIDi | 6883 |
KEGGi | hsa:6883 |
MANE-Selecti | ENST00000373824.9; ENSP00000362930.4; NM_005644.4; NP_005635.1 |
UCSCi | uc001bqx.5, human [Q16514-1] |
Organism-specific databases
CTDi | 6883 |
DisGeNETi | 6883 |
GeneCardsi | TAF12 |
HGNCi | HGNC:11545, TAF12 |
HPAi | ENSG00000120656, Low tissue specificity |
MIMi | 600773, gene |
neXtProti | NX_Q16514 |
OpenTargetsi | ENSG00000120656 |
PharmGKBi | PA36320 |
VEuPathDBi | HostDB:ENSG00000120656 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG1142, Eukaryota |
GeneTreei | ENSGT00390000002144 |
HOGENOMi | CLU_093619_3_1_1 |
InParanoidi | Q16514 |
OMAi | HRRDTTV |
OrthoDBi | 1569957at2759 |
PhylomeDBi | Q16514 |
TreeFami | TF323652 |
Enzyme and pathway databases
PathwayCommonsi | Q16514 |
Reactomei | R-HSA-167161, HIV Transcription Initiation R-HSA-167162, RNA Polymerase II HIV Promoter Escape R-HSA-167172, Transcription of the HIV genome R-HSA-3214847, HATs acetylate histones R-HSA-674695, RNA Polymerase II Pre-transcription Events R-HSA-6804756, Regulation of TP53 Activity through Phosphorylation R-HSA-73776, RNA Polymerase II Promoter Escape R-HSA-73779, RNA Polymerase II Transcription Pre-Initiation And Promoter Opening R-HSA-75953, RNA Polymerase II Transcription Initiation R-HSA-76042, RNA Polymerase II Transcription Initiation And Promoter Clearance |
SignaLinki | Q16514 |
SIGNORi | Q16514 |
Miscellaneous databases
BioGRID-ORCSi | 6883, 422 hits in 1081 CRISPR screens |
ChiTaRSi | TAF12, human |
EvolutionaryTracei | Q16514 |
GeneWikii | TAF12 |
GenomeRNAii | 6883 |
Pharosi | Q16514, Tbio |
PROi | PR:Q16514 |
RNActi | Q16514, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000120656, Expressed in oocyte and 222 other tissues |
Genevisiblei | Q16514, HS |
Family and domain databases
CDDi | cd07981, TAF12, 1 hit |
Gene3Di | 1.10.20.10, 1 hit |
IDEALi | IID00248 |
InterProi | View protein in InterPro IPR009072, Histone-fold IPR037794, TAF12 IPR003228, TFIID_TAF12_dom |
PANTHERi | PTHR12264, PTHR12264, 1 hit |
Pfami | View protein in Pfam PF03847, TFIID_20kDa, 1 hit |
SUPFAMi | SSF47113, SSF47113, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | TAF12_HUMAN | |
Accessioni | Q16514Primary (citable) accession number: Q16514 Secondary accession number(s): D3DPM5, Q15775, Q5T077 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 15, 1998 |
Last sequence update: | November 1, 1996 | |
Last modified: | May 25, 2022 | |
This is version 213 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- Human chromosome 1
Human chromosome 1: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families