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Protein

Transcription initiation factor TFIID subunit 12

Gene

TAF12

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

TAFs are components of the transcription factor IID (TFIID) complex, PCAF histone acetylase complex and TBP-free TAFII complex (TFTC). TAFs components-TIIFD are essential for mediating regulation of RNA polymerase transcription.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processTranscription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-HSA-167161 HIV Transcription Initiation
R-HSA-167162 RNA Polymerase II HIV Promoter Escape
R-HSA-167172 Transcription of the HIV genome
R-HSA-3214847 HATs acetylate histones
R-HSA-674695 RNA Polymerase II Pre-transcription Events
R-HSA-6804756 Regulation of TP53 Activity through Phosphorylation
R-HSA-73776 RNA Polymerase II Promoter Escape
R-HSA-73779 RNA Polymerase II Transcription Pre-Initiation And Promoter Opening
R-HSA-75953 RNA Polymerase II Transcription Initiation
R-HSA-76042 RNA Polymerase II Transcription Initiation And Promoter Clearance
SIGNORiQ16514

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription initiation factor TFIID subunit 12
Alternative name(s):
Transcription initiation factor TFIID 20/15 kDa subunits
Short name:
TAFII-20/TAFII-15
Short name:
TAFII20/TAFII15
Gene namesi
Name:TAF12
Synonyms:TAF15, TAF2J, TAFII20
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

EuPathDBiHostDB:ENSG00000120656.11
HGNCiHGNC:11545 TAF12
MIMi600773 gene
neXtProtiNX_Q16514

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi6883
OpenTargetsiENSG00000120656
PharmGKBiPA36320

Polymorphism and mutation databases

BioMutaiTAF12
DMDMi3024708

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000335801 – 161Transcription initiation factor TFIID subunit 12Add BLAST161

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki19Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei43PhosphothreonineCombined sources1
Modified residuei51PhosphoserineCombined sources1
Modified residuei59PhosphothreonineCombined sources1

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ16514
MaxQBiQ16514
PaxDbiQ16514
PeptideAtlasiQ16514
PRIDEiQ16514
ProteomicsDBi60885
60886 [Q16514-2]

PTM databases

iPTMnetiQ16514
PhosphoSitePlusiQ16514

Expressioni

Tissue specificityi

Ubiquitous.

Gene expression databases

BgeeiENSG00000120656 Expressed in 210 organ(s), highest expression level in oocyte
CleanExiHS_TAF12
HS_TAF15
GenevisibleiQ16514 HS

Organism-specific databases

HPAiHPA008519

Interactioni

Subunit structurei

TFIID and PCAF are composed of TATA binding protein (TBP) and a number of TBP-associated factors (TAFs). TBP is not part of TFTC. Interacts directly with TBP; additional interactions between TAFII20 and TAFII28 or TAFII30 were detected. Component of the PCAF complex, at least composed of TADA2L/ADA2, TADA3L/ADA3, TAF5L/PAF65-beta, SUPT3H, TAF6L, TAF9, TAF10, TAF12 and TRRAP. Component of the STAGA transcription coactivator-HAT complex, at least composed of SUPT3H, GCN5L2, TAF5L, TAF6L, STAF65-gamma/KIAA0764, TADA3L, TAD1L, TAF10, TAF12, TRRAP and TAF9. Interacts (isoform TAFII15 and isoform TAFII20) with ATF7 (via the transactivation domain); the interaction is prevented by sumoylation of ATF7 and promotes (isoform TAFII20 only) the transactivation of ATF7.5 Publications

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi112746, 68 interactors
ComplexPortaliCPX-900 SAGA complex
CPX-903 TFTC histone acetylation complex
CPX-915 General transcription factor complex TFIID
CPX-930 General transcription factor complex TFIID, TAF4B variant
CPX-989 PCAF histone acetylase complex
CORUMiQ16514
DIPiDIP-496N
IntActiQ16514, 28 interactors
MINTiQ16514
STRINGi9606.ENSP00000263974

Structurei

Secondary structure

1161
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ16514
SMRiQ16514
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ16514

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini59 – 126Histone-foldSequence analysisAdd BLAST68

Sequence similaritiesi

Belongs to the TAF12 family.Curated

Phylogenomic databases

eggNOGiKOG1142 Eukaryota
COG5624 LUCA
GeneTreeiENSGT00390000002144
HOGENOMiHOG000035092
HOVERGENiHBG055174
InParanoidiQ16514
KOiK03126
OMAiQASMANS
OrthoDBiEOG091G0Q1X
PhylomeDBiQ16514
TreeFamiTF323652

Family and domain databases

CDDicd07981 TAF12, 1 hit
Gene3Di1.10.20.10, 1 hit
InterProiView protein in InterPro
IPR009072 Histone-fold
IPR037794 TAF12
IPR003228 TFIID_TAF12_dom
PANTHERiPTHR12264 PTHR12264, 1 hit
PfamiView protein in Pfam
PF03847 TFIID_20kDa, 1 hit
ProDomiView protein in ProDom or Entries sharing at least one domain
PD012998 PD012998, 1 hit
SUPFAMiSSF47113 SSF47113, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative initiation. AlignAdd to basket
Isoform TAFII20 (identifier: Q16514-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MNQFGPSALI NLSNFSSIKP EPASTPPQGS MANSTAVVKI PGTPGAGGRL
60 70 80 90 100
SPENNQVLTK KKLQDLVREV DPNEQLDEDV EEMLLQIADD FIESVVTAAC
110 120 130 140 150
QLARHRKSST LEVKDVQLHL ERQWNMWIPG FGSEEIRPYK KACTTEAHKQ
160
RMALIRKTTK K
Length:161
Mass (Da):17,924
Last modified:November 1, 1996 - v1
Checksum:i054B017CD7BF99CE
GO
Isoform TAFII15 (identifier: Q16514-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-30: Missing.

Show »
Length:131
Mass (Da):14,825
Checksum:i655B769918311CE1
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0188881 – 30Missing in isoform TAFII15. 1 PublicationAdd BLAST30

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U57693 mRNA Translation: AAC50600.1
U57693 mRNA Translation: AAC50601.1
D50544 mRNA Translation: BAA09112.1
X84002 mRNA Translation: CAA58826.1
BT007031 mRNA Translation: AAP35678.1
AY206865 Genomic DNA Translation: AAO13491.1
AL513497 Genomic DNA No translation available.
CH471059 Genomic DNA Translation: EAX07682.1
CH471059 Genomic DNA Translation: EAX07683.1
BC011986 mRNA Translation: AAH11986.1
CCDSiCCDS326.1 [Q16514-1]
PIRiJC4676
RefSeqiNP_001128690.1, NM_001135218.1 [Q16514-1]
NP_005635.1, NM_005644.3 [Q16514-1]
UniGeneiHs.530251

Genome annotation databases

EnsembliENST00000263974; ENSP00000263974; ENSG00000120656 [Q16514-1]
ENST00000373824; ENSP00000362930; ENSG00000120656 [Q16514-1]
GeneIDi6883
KEGGihsa:6883
UCSCiuc001bqx.5 human [Q16514-1]

Keywords - Coding sequence diversityi

Alternative initiation

Similar proteinsi

Cross-referencesi

Web resourcesi

NIEHS-SNPs

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U57693 mRNA Translation: AAC50600.1
U57693 mRNA Translation: AAC50601.1
D50544 mRNA Translation: BAA09112.1
X84002 mRNA Translation: CAA58826.1
BT007031 mRNA Translation: AAP35678.1
AY206865 Genomic DNA Translation: AAO13491.1
AL513497 Genomic DNA No translation available.
CH471059 Genomic DNA Translation: EAX07682.1
CH471059 Genomic DNA Translation: EAX07683.1
BC011986 mRNA Translation: AAH11986.1
CCDSiCCDS326.1 [Q16514-1]
PIRiJC4676
RefSeqiNP_001128690.1, NM_001135218.1 [Q16514-1]
NP_005635.1, NM_005644.3 [Q16514-1]
UniGeneiHs.530251

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1H3OX-ray2.30B/D57-128[»]
ProteinModelPortaliQ16514
SMRiQ16514
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi112746, 68 interactors
ComplexPortaliCPX-900 SAGA complex
CPX-903 TFTC histone acetylation complex
CPX-915 General transcription factor complex TFIID
CPX-930 General transcription factor complex TFIID, TAF4B variant
CPX-989 PCAF histone acetylase complex
CORUMiQ16514
DIPiDIP-496N
IntActiQ16514, 28 interactors
MINTiQ16514
STRINGi9606.ENSP00000263974

PTM databases

iPTMnetiQ16514
PhosphoSitePlusiQ16514

Polymorphism and mutation databases

BioMutaiTAF12
DMDMi3024708

Proteomic databases

EPDiQ16514
MaxQBiQ16514
PaxDbiQ16514
PeptideAtlasiQ16514
PRIDEiQ16514
ProteomicsDBi60885
60886 [Q16514-2]

Protocols and materials databases

DNASUi6883
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000263974; ENSP00000263974; ENSG00000120656 [Q16514-1]
ENST00000373824; ENSP00000362930; ENSG00000120656 [Q16514-1]
GeneIDi6883
KEGGihsa:6883
UCSCiuc001bqx.5 human [Q16514-1]

Organism-specific databases

CTDi6883
DisGeNETi6883
EuPathDBiHostDB:ENSG00000120656.11
GeneCardsiTAF12
HGNCiHGNC:11545 TAF12
HPAiHPA008519
MIMi600773 gene
neXtProtiNX_Q16514
OpenTargetsiENSG00000120656
PharmGKBiPA36320
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1142 Eukaryota
COG5624 LUCA
GeneTreeiENSGT00390000002144
HOGENOMiHOG000035092
HOVERGENiHBG055174
InParanoidiQ16514
KOiK03126
OMAiQASMANS
OrthoDBiEOG091G0Q1X
PhylomeDBiQ16514
TreeFamiTF323652

Enzyme and pathway databases

ReactomeiR-HSA-167161 HIV Transcription Initiation
R-HSA-167162 RNA Polymerase II HIV Promoter Escape
R-HSA-167172 Transcription of the HIV genome
R-HSA-3214847 HATs acetylate histones
R-HSA-674695 RNA Polymerase II Pre-transcription Events
R-HSA-6804756 Regulation of TP53 Activity through Phosphorylation
R-HSA-73776 RNA Polymerase II Promoter Escape
R-HSA-73779 RNA Polymerase II Transcription Pre-Initiation And Promoter Opening
R-HSA-75953 RNA Polymerase II Transcription Initiation
R-HSA-76042 RNA Polymerase II Transcription Initiation And Promoter Clearance
SIGNORiQ16514

Miscellaneous databases

ChiTaRSiTAF12 human
EvolutionaryTraceiQ16514
GeneWikiiTAF12
GenomeRNAii6883
PROiPR:Q16514
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000120656 Expressed in 210 organ(s), highest expression level in oocyte
CleanExiHS_TAF12
HS_TAF15
GenevisibleiQ16514 HS

Family and domain databases

CDDicd07981 TAF12, 1 hit
Gene3Di1.10.20.10, 1 hit
InterProiView protein in InterPro
IPR009072 Histone-fold
IPR037794 TAF12
IPR003228 TFIID_TAF12_dom
PANTHERiPTHR12264 PTHR12264, 1 hit
PfamiView protein in Pfam
PF03847 TFIID_20kDa, 1 hit
ProDomiView protein in ProDom or Entries sharing at least one domain
PD012998 PD012998, 1 hit
SUPFAMiSSF47113 SSF47113, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiTAF12_HUMAN
AccessioniPrimary (citable) accession number: Q16514
Secondary accession number(s): D3DPM5, Q15775, Q5T077
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: November 1, 1996
Last modified: November 7, 2018
This is version 193 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  3. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  4. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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