UniProtKB - Q16352 (AINX_HUMAN)
Protein
Alpha-internexin
Gene
INA
Organism
Homo sapiens (Human)
Status
Functioni
Class-IV neuronal intermediate filament that is able to self-assemble. It is involved in the morphogenesis of neurons. It may form an independent structural network without the involvement of other neurofilaments or it may cooperate with NEFL to form the filamentous backbone to which NEFM and NEFH attach to form the cross-bridges. May also cooperate with the neuronal intermediate filament protein PRPH to form filamentous networks (By similarity).By similarity
GO - Molecular functioni
- structural constituent of cytoskeleton Source: ProtInc
- structural constituent of postsynaptic actin cytoskeleton Source: Ensembl
- structural constituent of postsynaptic intermediate filament cytoskeleton Source: Ensembl
GO - Biological processi
- cell differentiation Source: UniProtKB-KW
- cellular response to leukemia inhibitory factor Source: Ensembl
- neurofilament cytoskeleton organization Source: Ensembl
- substantia nigra development Source: UniProtKB
Keywordsi
Molecular function | Developmental protein |
Biological process | Differentiation, Neurogenesis |
Enzyme and pathway databases
PathwayCommonsi | Q16352 |
Names & Taxonomyi
Protein namesi | Recommended name: Alpha-internexinShort name: Alpha-Inx Alternative name(s): 66 kDa neurofilament protein Short name: NF-66 Short name: Neurofilament-66 Neurofilament 5 |
Gene namesi | Name:INA Synonyms:NEF5 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
EuPathDBi | HostDB:ENSG00000148798.9 |
HGNCi | HGNC:6057, INA |
MIMi | 605338, gene |
neXtProti | NX_Q16352 |
Subcellular locationi
Cytoskeleton
- intermediate filament cytoskeleton Source: HPA
- neurofilament Source: ProtInc
Extracellular region or secreted
- extracellular space Source: UniProtKB
Other locations
- cytoplasmic ribonucleoprotein granule Source: ParkinsonsUK-UCL
- postsynapse Source: Ensembl
- Schaffer collateral - CA1 synapse Source: Ensembl
Keywords - Cellular componenti
Intermediate filamentPathology & Biotechi
Organism-specific databases
DisGeNETi | 9118 |
OpenTargetsi | ENSG00000148798 |
PharmGKBi | PA29867 |
Miscellaneous databases
Pharosi | Q16352, Tbio |
Polymorphism and mutation databases
BioMutai | INA |
DMDMi | 20141266 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000063783 | 1 – 499 | Alpha-internexinAdd BLAST | 499 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 72 | PhosphoserineBy similarity | 1 | |
Modified residuei | 219 | PhosphoserineBy similarity | 1 | |
Modified residuei | 290 | N6-acetyllysineCombined sources | 1 | |
Modified residuei | 335 | PhosphoserineBy similarity | 1 | |
Modified residuei | 469 | PhosphoserineBy similarity | 1 | |
Modified residuei | 496 | PhosphoserineCombined sources | 1 |
Post-translational modificationi
O-glycosylated.By similarity
Keywords - PTMi
Acetylation, Glycoprotein, PhosphoproteinProteomic databases
EPDi | Q16352 |
jPOSTi | Q16352 |
MassIVEi | Q16352 |
MaxQBi | Q16352 |
PaxDbi | Q16352 |
PeptideAtlasi | Q16352 |
PRIDEi | Q16352 |
ProteomicsDBi | 60861 |
PTM databases
iPTMneti | Q16352 |
PhosphoSitePlusi | Q16352 |
SwissPalmi | Q16352 |
Expressioni
Tissue specificityi
Found predominantly in adult CNS.
Developmental stagei
Expressed in brain as early as the 16th week of gestation, and increased rapidly and reached a steady state level by the 18th week of gestation.
Gene expression databases
Bgeei | ENSG00000148798, Expressed in cortical plate and 154 other tissues |
Genevisiblei | Q16352, HS |
Organism-specific databases
HPAi | ENSG00000148798, Tissue enriched (brain) |
Interactioni
Subunit structurei
Forms homodimers (in vitro) (By similarity). Forms heterodimers with NEFL, NEFM or NEFH (in vitro) (By similarity).
By similarityProtein-protein interaction databases
BioGRIDi | 114566, 54 interactors |
IntActi | Q16352, 38 interactors |
MINTi | Q16352 |
STRINGi | 9606.ENSP00000358865 |
Miscellaneous databases
RNActi | Q16352, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 94 – 407 | IF rodPROSITE-ProRule annotationAdd BLAST | 314 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 87 | HeadAdd BLAST | 87 | |
Regioni | 88 – 129 | Coil 1AAdd BLAST | 42 | |
Regioni | 130 – 142 | Linker 1Add BLAST | 13 | |
Regioni | 143 – 238 | Coil 1BAdd BLAST | 96 | |
Regioni | 239 – 262 | Linker 2Add BLAST | 24 | |
Regioni | 263 – 408 | Coil 2Add BLAST | 146 | |
Regioni | 409 – 499 | TailAdd BLAST | 91 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 449 – 454 | Poly-Glu | 6 |
Sequence similaritiesi
Belongs to the intermediate filament family.PROSITE-ProRule annotation
Keywords - Domaini
Coiled coilPhylogenomic databases
eggNOGi | ENOG502RAU0, Eukaryota |
GeneTreei | ENSGT00940000154418 |
HOGENOMi | CLU_012560_7_3_1 |
InParanoidi | Q16352 |
OMAi | EHYLCSA |
OrthoDBi | 888678at2759 |
PhylomeDBi | Q16352 |
TreeFami | TF330122 |
Family and domain databases
Gene3Di | 1.20.5.1160, 1 hit |
InterProi | View protein in InterPro IPR027703, Alpha-Inx IPR018039, IF_conserved IPR039008, IF_rod_dom IPR042180, IF_rod_dom_coil1B IPR006821, Intermed_filament_DNA-bd |
PANTHERi | PTHR45652:SF18, PTHR45652:SF18, 1 hit |
Pfami | View protein in Pfam PF00038, Filament, 1 hit PF04732, Filament_head, 1 hit |
SMARTi | View protein in SMART SM01391, Filament, 1 hit |
PROSITEi | View protein in PROSITE PS00226, IF_ROD_1, 1 hit PS51842, IF_ROD_2, 1 hit |
i Sequence
Sequence statusi: Complete.
Q16352-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSFGSEHYLC SSSSYRKVFG DGSRLSARLS GAGGAGGFRS QSLSRSNVAS
60 70 80 90 100
SAACSSASSL GLGLAYRRPP ASDGLDLSQA AARTNEYKII RTNEKEQLQG
110 120 130 140 150
LNDRFAVFIE KVHQLETQNR ALEAELAALR QRHAEPSRVG ELFQRELRDL
160 170 180 190 200
RAQLEEASSA RSQALLERDG LAEEVQRLRA RCEEESRGRE GAERALKAQQ
210 220 230 240 250
RDVDGATLAR LDLEKKVESL LDELAFVRQV HDEEVAELLA TLQASSQAAA
260 270 280 290 300
EVDVTVAKPD LTSALREIRA QYESLAAKNL QSAEEWYKSK FANLNEQAAR
310 320 330 340 350
STEAIRASRE EIHEYRRQLQ ARTIEIEGLR GANESLERQI LELEERHSAE
360 370 380 390 400
VAGYQDSIGQ LENDLRNTKS EMARHLREYQ DLLNVKMALD IEIAAYRKLL
410 420 430 440 450
EGEETRFSTS GLSISGLNPL PNPSYLLPPR ILSATTSKVS STGLSLKKEE
460 470 480 490
EEEEASKVAS KKTSQIGESF EEILEETVIS TKKTEKSNIE ETTISSQKI
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 37 – 41 | GFRSQ → ASVE in AAB34482 (PubMed:7769995).Curated | 5 | |
Sequence conflicti | 67 | R → A in AAB34482 (PubMed:7769995).Curated | 1 | |
Sequence conflicti | 128 – 132 | ALRQR → RCDT in AAB34482 (PubMed:7769995).Curated | 5 | |
Sequence conflicti | 141 | E → Q in AAB34482 (PubMed:7769995).Curated | 1 | |
Sequence conflicti | 147 – 152 | LRDLRA → PRHLP in AAB34482 (PubMed:7769995).Curated | 6 | |
Sequence conflicti | 191 – 198 | GAERALKA → RRARLKR in AAB34482 (PubMed:7769995).Curated | 8 | |
Sequence conflicti | 244 | A → R in AAB34482 (PubMed:7769995).Curated | 1 | |
Sequence conflicti | 263 | S → A in AAB34482 (PubMed:7769995).Curated | 1 | |
Sequence conflicti | 301 | S → T in AAB34482 (PubMed:7769995).Curated | 1 | |
Sequence conflicti | 310 – 311 | EE → DQ in AAB34482 (PubMed:7769995).Curated | 2 | |
Sequence conflicti | 318 | Missing in AAB34482 (PubMed:7769995).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_049808 | 92 | T → S1 PublicationCorresponds to variant dbSNP:rs1063455Ensembl. | 1 | |
Natural variantiVAR_036369 | 110 | E → Q in a breast cancer sample; somatic mutation. 1 Publication | 1 | |
Natural variantiVAR_033497 | 149 | D → H. Corresponds to variant dbSNP:rs1063456Ensembl. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | S78296 mRNA Translation: AAB34482.1 AL591408 Genomic DNA No translation available. CH471066 Genomic DNA Translation: EAW49653.1 BC006359 mRNA Translation: AAH06359.1 |
CCDSi | CCDS7545.1 |
PIRi | I52658 |
RefSeqi | NP_116116.1, NM_032727.3 |
Genome annotation databases
Ensembli | ENST00000369849; ENSP00000358865; ENSG00000148798 |
GeneIDi | 9118 |
KEGGi | hsa:9118 |
UCSCi | uc001kws.3, human |
Keywords - Coding sequence diversityi
PolymorphismSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | S78296 mRNA Translation: AAB34482.1 AL591408 Genomic DNA No translation available. CH471066 Genomic DNA Translation: EAW49653.1 BC006359 mRNA Translation: AAH06359.1 |
CCDSi | CCDS7545.1 |
PIRi | I52658 |
RefSeqi | NP_116116.1, NM_032727.3 |
3D structure databases
SMRi | Q16352 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 114566, 54 interactors |
IntActi | Q16352, 38 interactors |
MINTi | Q16352 |
STRINGi | 9606.ENSP00000358865 |
PTM databases
iPTMneti | Q16352 |
PhosphoSitePlusi | Q16352 |
SwissPalmi | Q16352 |
Polymorphism and mutation databases
BioMutai | INA |
DMDMi | 20141266 |
Proteomic databases
EPDi | Q16352 |
jPOSTi | Q16352 |
MassIVEi | Q16352 |
MaxQBi | Q16352 |
PaxDbi | Q16352 |
PeptideAtlasi | Q16352 |
PRIDEi | Q16352 |
ProteomicsDBi | 60861 |
Protocols and materials databases
Antibodypediai | 1538, 473 antibodies |
DNASUi | 9118 |
Genome annotation databases
Ensembli | ENST00000369849; ENSP00000358865; ENSG00000148798 |
GeneIDi | 9118 |
KEGGi | hsa:9118 |
UCSCi | uc001kws.3, human |
Organism-specific databases
CTDi | 9118 |
DisGeNETi | 9118 |
EuPathDBi | HostDB:ENSG00000148798.9 |
GeneCardsi | INA |
HGNCi | HGNC:6057, INA |
HPAi | ENSG00000148798, Tissue enriched (brain) |
MIMi | 605338, gene |
neXtProti | NX_Q16352 |
OpenTargetsi | ENSG00000148798 |
PharmGKBi | PA29867 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | ENOG502RAU0, Eukaryota |
GeneTreei | ENSGT00940000154418 |
HOGENOMi | CLU_012560_7_3_1 |
InParanoidi | Q16352 |
OMAi | EHYLCSA |
OrthoDBi | 888678at2759 |
PhylomeDBi | Q16352 |
TreeFami | TF330122 |
Enzyme and pathway databases
PathwayCommonsi | Q16352 |
Miscellaneous databases
BioGRID-ORCSi | 9118, 3 hits in 843 CRISPR screens |
ChiTaRSi | INA, human |
GenomeRNAii | 9118 |
Pharosi | Q16352, Tbio |
PROi | PR:Q16352 |
RNActi | Q16352, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000148798, Expressed in cortical plate and 154 other tissues |
Genevisiblei | Q16352, HS |
Family and domain databases
Gene3Di | 1.20.5.1160, 1 hit |
InterProi | View protein in InterPro IPR027703, Alpha-Inx IPR018039, IF_conserved IPR039008, IF_rod_dom IPR042180, IF_rod_dom_coil1B IPR006821, Intermed_filament_DNA-bd |
PANTHERi | PTHR45652:SF18, PTHR45652:SF18, 1 hit |
Pfami | View protein in Pfam PF00038, Filament, 1 hit PF04732, Filament_head, 1 hit |
SMARTi | View protein in SMART SM01391, Filament, 1 hit |
PROSITEi | View protein in PROSITE PS00226, IF_ROD_1, 1 hit PS51842, IF_ROD_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | AINX_HUMAN | |
Accessioni | Q16352Primary (citable) accession number: Q16352 Secondary accession number(s): B1AQK0, Q9BRC5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 30, 2000 |
Last sequence update: | January 23, 2002 | |
Last modified: | December 2, 2020 | |
This is version 185 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- Human polymorphisms and disease mutations
Index of human polymorphisms and disease mutations - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families - Human chromosome 10
Human chromosome 10: entries, gene names and cross-references to MIM - Human entries with polymorphisms or disease mutations
List of human entries with polymorphisms or disease mutations