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Protein

Transcription factor E2F4

Gene

E2F4

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F4 binds with high affinity to RBL1 and RBL2. In some instances can also bind RB1. Specifically required for multiciliate cell differentiation: together with MCIDAS and E2F5, binds and activate genes required for centriole biogenesis.By similarity2 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi16 – 85Sequence analysisAdd BLAST70

GO - Molecular functioni

  • DNA binding Source: UniProtKB
  • DNA binding transcription factor activity Source: UniProtKB
  • promoter-specific chromatin binding Source: MGI
  • protein dimerization activity Source: InterPro
  • protein domain specific binding Source: UniProtKB
  • RNA polymerase II proximal promoter sequence-specific DNA binding Source: NTNU_SB
  • RNA polymerase II transcription factor activity, sequence-specific DNA binding Source: NTNU_SB
  • transcriptional activator activity, RNA polymerase II proximal promoter sequence-specific DNA binding Source: NTNU_SB
  • transcription factor binding Source: UniProtKB

GO - Biological processi

Keywordsi

Molecular functionActivator, DNA-binding
Biological processCell cycle, Cilium biogenesis/degradation, Transcription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-HSA-1362277 Transcription of E2F targets under negative control by DREAM complex
R-HSA-1362300 Transcription of E2F targets under negative control by p107 (RBL1) and p130 (RBL2) in complex with HDAC1
R-HSA-1538133 G0 and Early G1
R-HSA-2173796 SMAD2/SMAD3:SMAD4 heterotrimer regulates transcription
R-HSA-539107 Activation of E2F1 target genes at G1/S
R-HSA-6804114 TP53 Regulates Transcription of Genes Involved in G2 Cell Cycle Arrest
R-HSA-69202 Cyclin E associated events during G1/S transition
R-HSA-69231 Cyclin D associated events in G1
R-HSA-69656 Cyclin A:Cdk2-associated events at S phase entry
SignaLinkiQ16254
SIGNORiQ16254

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription factor E2F4
Short name:
E2F-4
Gene namesi
Name:E2F4
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 16

Organism-specific databases

EuPathDBiHostDB:ENSG00000205250.8
HGNCiHGNC:3118 E2F4
MIMi600659 gene
neXtProtiNX_Q16254

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi1874
OpenTargetsiENSG00000205250
PharmGKBiPA27576

Polymorphism and mutation databases

BioMutaiE2F4
DMDMi2494229

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00002194682 – 413Transcription factor E2F4Add BLAST412

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineCombined sources1
Modified residuei384PhosphoserineCombined sources1

Post-translational modificationi

Differentially phosphorylated in vivo.

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ16254
MaxQBiQ16254
PaxDbiQ16254
PeptideAtlasiQ16254
PRIDEiQ16254
ProteomicsDBi60845

PTM databases

iPTMnetiQ16254
PhosphoSitePlusiQ16254

Expressioni

Tissue specificityi

Found in all tissue examined including heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.

Developmental stagei

Present in the growth-arrested state, its abundance does not change significantly as cells move into and through the cell cycle.

Gene expression databases

BgeeiENSG00000205250
CleanExiHS_E2F4
ExpressionAtlasiQ16254 baseline and differential
GenevisibleiQ16254 HS

Organism-specific databases

HPAiHPA054128

Interactioni

Subunit structurei

Component of the DRTF1/E2F transcription factor complex. Binds cooperatively with TFDP1/Dp-1 to E2F sites. The E2F4/TFDP1 dimer interacts preferentially with pocket protein RBL1, which inhibits the E2F transactivation domain. Lower affinity interaction has been found with retinoblastoma protein RB1. Interacts with TRRAP, which probably mediates its interaction with histone acetyltransferase complexes, leading to transcription activation. Interacts with HCFC1. Component of the DREAM complex (also named LINC complex) at least composed of E2F4, E2F5, LIN9, LIN37, LIN52, LIN54, MYBL1, MYBL2, RBL1, RBL2, RBBP4, TFDP1 and TFDP2. The complex exists in quiescent cells where it represses cell cycle-dependent genes. It dissociates in S phase when LIN9, LIN37, LIN52 and LIN54 form a subcomplex that binds to MYBL2. Interacts with PML (isoform PML-1, isoform PML-2, isoform PML-3, isoform PML-4 and isoform PML-5). Interacts with CEBPA (when phosphorylated) (PubMed:15107404).10 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

  • protein dimerization activity Source: InterPro
  • protein domain specific binding Source: UniProtKB
  • transcription factor binding Source: UniProtKB

Protein-protein interaction databases

BioGridi108206, 50 interactors
CORUMiQ16254
DIPiDIP-24185N
ELMiQ16254
IntActiQ16254, 26 interactors
MINTiQ16254
STRINGi9606.ENSP00000368686

Structurei

Secondary structure

1413
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni17 – 20Combined sources4
Helixi22 – 35Combined sources14
Beta strandi40 – 42Combined sources3
Helixi43 – 49Combined sources7
Turni50 – 52Combined sources3
Helixi56 – 68Combined sources13
Beta strandi70 – 75Combined sources6
Beta strandi78 – 81Combined sources4
Helixi96 – 129Combined sources34
Helixi132 – 134Combined sources3
Beta strandi139 – 141Combined sources3
Helixi142 – 148Combined sources7
Turni149 – 151Combined sources3
Beta strandi152 – 158Combined sources7
Beta strandi164 – 166Combined sources3
Beta strandi179 – 184Combined sources6
Beta strandi186 – 188Combined sources3
Beta strandi191 – 194Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1CF7X-ray2.60A11-86[»]
5TUUX-ray2.25B91-198[»]
ProteinModelPortaliQ16254
SMRiQ16254
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ16254

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni43 – 65Leucine-zipperAdd BLAST23
Regioni86 – 181DimerizationSequence analysisAdd BLAST96
Regioni337 – 413TransactivationSequence analysisAdd BLAST77
Regioni390 – 407Interaction with RBL1 and RBL2Sequence analysisAdd BLAST18

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi48 – 85DEF boxAdd BLAST38
Motifi389 – 392HCFC1-binding-motif (HBM)4

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi9 – 12Poly-Pro4
Compositional biasi307 – 327Poly-SerAdd BLAST21

Sequence similaritiesi

Belongs to the E2F/DP family.Curated

Phylogenomic databases

eggNOGiKOG2577 Eukaryota
ENOG410XNYI LUCA
GeneTreeiENSGT00550000074403
HOGENOMiHOG000232045
HOVERGENiHBG002227
InParanoidiQ16254
KOiK04682
OMAiVQNSPHT
OrthoDBiEOG091G0CJQ
PhylomeDBiQ16254
TreeFamiTF105566

Family and domain databases

CDDicd14660 E2F_DD, 1 hit
Gene3Di1.10.10.10, 1 hit
InterProiView protein in InterPro
IPR015633 E2F
IPR037241 E2F-DP_heterodim
IPR028312 E2F4
IPR032198 E2F_CC-MB
IPR003316 E2F_WHTH_DNA-bd_dom
IPR036388 WH-like_DNA-bd_sf
IPR036390 WH_DNA-bd_sf
PANTHERiPTHR12081 PTHR12081, 1 hit
PTHR12081:SF42 PTHR12081:SF42, 1 hit
PfamiView protein in Pfam
PF16421 E2F_CC-MB, 1 hit
PF02319 E2F_TDP, 1 hit
SMARTiView protein in SMART
SM01372 E2F_TDP, 1 hit
SUPFAMiSSF144074 SSF144074, 1 hit
SSF46785 SSF46785, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q16254-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAEAGPQAPP PPGTPSRHEK SLGLLTTKFV SLLQEAKDGV LDLKLAADTL
60 70 80 90 100
AVRQKRRIYD ITNVLEGIGL IEKKSKNSIQ WKGVGPGCNT REIADKLIEL
110 120 130 140 150
KAEIEELQQR EQELDQHKVW VQQSIRNVTE DVQNSCLAYV THEDICRCFA
160 170 180 190 200
GDTLLAIRAP SGTSLEVPIP EGLNGQKKYQ IHLKSVSGPI EVLLVNKEAW
210 220 230 240 250
SSPPVAVPVP PPEDLLQSPS AVSTPPPLPK PALAQSQEAS RPNSPQLTPT
260 270 280 290 300
AVPGSAEVQG MAGPAAEITV SGGPGTDSKD SGELSSLPLG PTTLDTRPLQ
310 320 330 340 350
SSALLDSSSS SSSSSSSSSN SNSSSSSGPN PSTSFEPIKA DPTGVLELPK
360 370 380 390 400
ELSEIFDPTR ECMSSELLEE LMSSEVFAPL LRLSPPPGDH DYIYNLDESE
410
GVCDLFDVPV LNL
Length:413
Mass (Da):43,960
Last modified:November 1, 1997 - v2
Checksum:iBAAC95DE1B7E0832
GO

Polymorphismi

The poly-Ser region of E2F4 is polymorphic and the number of Ser varies in the population (from 8 to 17). The variation might be associated with tumorigenesis.

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_014936293T → P. Corresponds to variant dbSNP:rs1801013Ensembl.1
Natural variantiVAR_014024319S → SSSS. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S75174 mRNA Translation: AAB32597.1
X86096 mRNA Translation: CAA60050.2
U15641 mRNA Translation: AAC50119.1
AF250378 Genomic DNA Translation: AAF65226.1
AB451292 mRNA Translation: BAG70106.1
AB451425 mRNA Translation: BAG70239.1
AF527540 Genomic DNA Translation: AAM77918.1
AC040160 Genomic DNA No translation available.
CH471092 Genomic DNA Translation: EAW83094.1
BC033180 mRNA Translation: AAH33180.1
CCDSiCCDS32464.1
PIRiA55237
RefSeqiNP_001941.2, NM_001950.3
UniGeneiHs.108371

Genome annotation databases

EnsembliENST00000379378; ENSP00000368686; ENSG00000205250
GeneIDi1874
KEGGihsa:1874
UCSCiuc002erz.4 human

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Entry informationi

Entry nameiE2F4_HUMAN
AccessioniPrimary (citable) accession number: Q16254
Secondary accession number(s): A6NGR8
, B5BU56, Q12991, Q15328
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: June 20, 2018
This is version 189 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 16
    Human chromosome 16: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

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