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Protein

Succinyl-diaminopimelate desuccinylase

Gene

dapE

Organism
Pseudoalteromonas atlantica (strain T6c / ATCC BAA-1087)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the hydrolysis of N-succinyl-L,L-diaminopimelic acid (SDAP), forming succinate and LL-2,6-diaminoheptanedioate (DAP), an intermediate involved in the bacterial biosynthesis of lysine and meso-diaminopimelic acid, an essential component of bacterial cell walls.UniRule annotation

Catalytic activityi

N-succinyl-LL-2,6-diaminoheptanedioate + H2O = succinate + LL-2,6-diaminoheptanedioate.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • Zn2+UniRule annotationNote: Binds 1 Zn2+ ion per subunit.UniRule annotation
  • Co2+UniRule annotationNote: Binds 1 Co2+ ion per subunit.UniRule annotation

Pathwayi: L-lysine biosynthesis via DAP pathway

This protein is involved in step 3 of the subpathway that synthesizes LL-2,6-diaminopimelate from (S)-tetrahydrodipicolinate (succinylase route).UniRule annotation
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase (dapD)
  2. no protein annotated in this organism
  3. Succinyl-diaminopimelate desuccinylase (dapE)
This subpathway is part of the pathway L-lysine biosynthesis via DAP pathway, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes LL-2,6-diaminopimelate from (S)-tetrahydrodipicolinate (succinylase route), the pathway L-lysine biosynthesis via DAP pathway and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi67Cobalt or zinc 1UniRule annotation1
Active sitei69UniRule annotation1
Metal bindingi100Cobalt or zinc 1UniRule annotation1
Metal bindingi100Cobalt or zinc 2UniRule annotation1
Active sitei134Proton acceptorUniRule annotation1
Metal bindingi135Cobalt or zinc 2UniRule annotation1
Metal bindingi163Cobalt or zinc 1UniRule annotation1
Metal bindingi349Cobalt or zinc 2UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase
Biological processAmino-acid biosynthesis, Diaminopimelate biosynthesis, Lysine biosynthesis
LigandCobalt, Metal-binding, Zinc

Enzyme and pathway databases

BioCyciPATL342610:G1G6R-2259-MONOMER
UniPathwayiUPA00034; UER00021

Protein family/group databases

MEROPSiM20.010

Names & Taxonomyi

Protein namesi
Recommended name:
Succinyl-diaminopimelate desuccinylaseUniRule annotation (EC:3.5.1.18UniRule annotation)
Short name:
SDAP desuccinylaseUniRule annotation
Alternative name(s):
N-succinyl-LL-2,6-diaminoheptanedioate amidohydrolaseUniRule annotation
Gene namesi
Name:dapEUniRule annotation
Ordered Locus Names:Patl_2192
OrganismiPseudoalteromonas atlantica (strain T6c / ATCC BAA-1087)
Taxonomic identifieri342610 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesPseudoalteromonadaceaePseudoalteromonas
Proteomesi
  • UP000001981 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003756511 – 376Succinyl-diaminopimelate desuccinylaseAdd BLAST376

Proteomic databases

PRIDEiQ15TS8

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi342610.Patl_2192

Structurei

3D structure databases

ProteinModelPortaliQ15TS8
SMRiQ15TS8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase M20A family. DapE subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C9Y Bacteria
COG0624 LUCA
HOGENOMiHOG000243770
KOiK01439
OMAiLNSHHDT
OrthoDBiPOG091H059V

Family and domain databases

CDDicd03891 M20_DapE_proteobac, 1 hit
HAMAPiMF_01690 DapE, 1 hit
InterProiView protein in InterPro
IPR001261 ArgE/DapE_CS
IPR036264 Bact_exopeptidase_dim_dom
IPR005941 DapE_proteobac
IPR002933 Peptidase_M20
IPR011650 Peptidase_M20_dimer
PfamiView protein in Pfam
PF07687 M20_dimer, 1 hit
PF01546 Peptidase_M20, 1 hit
SUPFAMiSSF55031 SSF55031, 1 hit
TIGRFAMsiTIGR01246 dapE_proteo, 1 hit
PROSITEiView protein in PROSITE
PS00759 ARGE_DAPE_CPG2_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q15TS8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSCEVLALTE ELINRQSVTP EDAGCQQLMA EYLAPLGFDI ESMVFDDTTN
60 70 80 90 100
MWARKGTGGP VFCFAGHTDV VPSGPAEKWT FPPFTATQHE GQLYGRGAAD
110 120 130 140 150
MKGSLAAMLV ATKAFVTKHP EHSGSIAFLI TSDEEGPFIN GTTRVIDTLE
160 170 180 190 200
ARNEKMTWCL VGEPSSTTLI GDVVKNGRRG SLTGDITVKG VQGHVAYPHL
210 220 230 240 250
AKNPIHLSAP AFAELAQTHW DSGNASFPPT SFQVSNINSG TGAGNVIPGD
260 270 280 290 300
LSACFNFRFS TEVTDKQLIE RVTTILDKYD FDYHIDWTFN GQPFLTDSGK
310 320 330 340 350
LVEATQSAIK DVTGRETELS TAGGTSDGRF IAPTGAQVIE LGPINATIHK
360 370
IDENVNINDL AQLAKIYEGI LQRLLA
Length:376
Mass (Da):40,514
Last modified:July 25, 2006 - v1
Checksum:iC9C3BB3D5AAB1A35
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000388 Genomic DNA Translation: ABG40710.1
RefSeqiWP_011574994.1, NC_008228.1

Genome annotation databases

EnsemblBacteriaiABG40710; ABG40710; Patl_2192
KEGGipat:Patl_2192

Similar proteinsi

Entry informationi

Entry nameiDAPE_PSEA6
AccessioniPrimary (citable) accession number: Q15TS8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 26, 2009
Last sequence update: July 25, 2006
Last modified: July 18, 2018
This is version 84 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

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