UniProtKB - Q15853 (USF2_HUMAN)
Upstream stimulatory factor 2
USF2
Functioni
GO - Molecular functioni
- bHLH transcription factor binding Source: BHF-UCL
- DNA-binding transcription factor activity Source: ProtInc
- DNA-binding transcription factor activity, RNA polymerase II-specific Source: BHF-UCL
- protein heterodimerization activity Source: BHF-UCL
- protein homodimerization activity Source: BHF-UCL
- sequence-specific DNA binding Source: BHF-UCL
- sequence-specific double-stranded DNA binding Source: ARUK-UCL
GO - Biological processi
- late viral transcription Source: BHF-UCL
- lipid homeostasis Source: BHF-UCL
- positive regulation of transcription by RNA polymerase II Source: BHF-UCL
- positive regulation of transcription from RNA polymerase II promoter by glucose Source: BHF-UCL
- regulation of transcription from RNA polymerase II promoter by glucose Source: BHF-UCL
Keywordsi
Molecular function | DNA-binding |
Biological process | Transcription, Transcription regulation |
Enzyme and pathway databases
PathwayCommonsi | Q15853 |
Reactomei | R-HSA-9018519, Estrogen-dependent gene expression |
SignaLinki | Q15853 |
SIGNORi | Q15853 |
Names & Taxonomyi
Protein namesi | Recommended name: Upstream stimulatory factor 2Alternative name(s): Class B basic helix-loop-helix protein 12 Short name: bHLHb12 FOS-interacting protein Short name: FIP Major late transcription factor 2 Upstream transcription factor 2 |
Gene namesi | Name:USF2 Synonyms:BHLHB12 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:12594, USF2 |
MIMi | 600390, gene |
neXtProti | NX_Q15853 |
VEuPathDBi | HostDB:ENSG00000105698.15 |
Subcellular locationi
Nucleus
Nucleus
- nuclear chromatin Source: NTNU_SB
- nucleoplasm Source: HPA
- nucleus Source: BHF-UCL
Other locations
- intracellular membrane-bounded organelle Source: HPA
Keywords - Cellular componenti
NucleusPathology & Biotechi
Organism-specific databases
DisGeNETi | 7392 |
OpenTargetsi | ENSG00000105698 |
PharmGKBi | PA37224 |
Miscellaneous databases
Pharosi | Q15853, Tbio |
Genetic variation databases
BioMutai | USF2 |
DMDMi | 2833271 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000127500 | 1 – 346 | Upstream stimulatory factor 2Add BLAST | 346 |
Proteomic databases
EPDi | Q15853 |
jPOSTi | Q15853 |
MassIVEi | Q15853 |
MaxQBi | Q15853 |
PaxDbi | Q15853 |
PeptideAtlasi | Q15853 |
PRIDEi | Q15853 |
ProteomicsDBi | 60794 [Q15853-1] 60795 [Q15853-2] 60796 [Q15853-3] 67853 |
PTM databases
iPTMneti | Q15853 |
MetOSitei | Q15853 |
PhosphoSitePlusi | Q15853 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSG00000105698, Expressed in frontal cortex and 247 other tissues |
ExpressionAtlasi | Q15853, baseline and differential |
Genevisiblei | Q15853, HS |
Organism-specific databases
HPAi | ENSG00000105698, Low tissue specificity |
Interactioni
Subunit structurei
Interacts with MAF (By similarity). Efficient DNA binding requires dimerization with another bHLH protein. Binds DNA as a homodimer or a heterodimer (USF1/USF2). In vivo, the USF1/USF2A heterodimer represents over 66% of the usf binding activity whereas the USF1 and USF2A homodimers represent less than 10%. The USF1/USF2B heterodimer accounted for almost 15% in some cell.
By similarityBinary interactionsi
Hide detailsQ15853
With | #Exp. | IntAct |
---|---|---|
FOS [Q6FG41] | 3 | EBI-1055994,EBI-10198738 |
LZTS2 [Q9BRK4] | 3 | EBI-1055994,EBI-741037 |
TEAD2 [Q15562] | 3 | EBI-1055994,EBI-6427252 |
GO - Molecular functioni
- bHLH transcription factor binding Source: BHF-UCL
- protein heterodimerization activity Source: BHF-UCL
- protein homodimerization activity Source: BHF-UCL
Protein-protein interaction databases
BioGRIDi | 113238, 35 interactors |
ComplexPortali | CPX-3079, USF1-USF2 upstream stimulatory factor complex CPX-3083, USF2 upstream stimulatory factor complex |
CORUMi | Q15853 |
IntActi | Q15853, 19 interactors |
MINTi | Q15853 |
STRINGi | 9606.ENSP00000222305 |
Miscellaneous databases
RNActi | Q15853, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 235 – 290 | bHLHPROSITE-ProRule annotationAdd BLAST | 56 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 307 – 328 | Leucine-zipperAdd BLAST | 22 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 11 – 20 | Poly-Ala | 10 | |
Compositional biasi | 245 – 248 | Poly-Arg | 4 |
Phylogenomic databases
eggNOGi | KOG1318, Eukaryota |
GeneTreei | ENSGT00940000160704 |
HOGENOMi | CLU_070485_2_0_1 |
InParanoidi | Q15853 |
OrthoDBi | 1345445at2759 |
PhylomeDBi | Q15853 |
TreeFami | TF323338 |
Family and domain databases
CDDi | cd00083, HLH, 1 hit |
Gene3Di | 4.10.280.10, 1 hit |
InterProi | View protein in InterPro IPR011598, bHLH_dom IPR036638, HLH_DNA-bd_sf |
Pfami | View protein in Pfam PF00010, HLH, 1 hit |
SMARTi | View protein in SMART SM00353, HLH, 1 hit |
SUPFAMi | SSF47459, SSF47459, 1 hit |
PROSITEi | View protein in PROSITE PS50888, BHLH, 1 hit |
s (4+)i Sequence
Sequence statusi: Complete.
This entry describes 4 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 4 described isoforms and 7 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MDMLDPGLDP AASATAAAAA SHDKGPEAEE GVELQEGGDG PGAEEQTAVA
60 70 80 90 100
ITSVQQAAFG DHNIQYQFRT ETNGGQVTYR VVQVTDGQLD GQGDTAGAVS
110 120 130 140 150
VVSTAAFAGG QQAVTQVGVD GAAQRPGPAA ASVPPGPAAP FPLAVIQNPF
160 170 180 190 200
SNGGSPAAEA VSGEARFAYF PASSVGDTTA VSVQTTDQSL QAGGQFYVMM
210 220 230 240 250
TPQDVLQTGT QRTIAPRTHP YSPKIDGTRT PRDERRRAQH NEVERRRRDK
260 270 280 290 300
INNWIVQLSK IIPDCNADNS KTGASKGGIL SKACDYIREL RQTNQRMQET
310 320 330 340
FKEAERLQMD NELLRQQIEE LKNENALLRA QLQQHNLEMV GEGTRQ
The sequence of this isoform differs from the canonical sequence as follows:
275-282: Missing.
The sequence of this isoform differs from the canonical sequence as follows:
77-143: Missing.
The sequence of this isoform differs from the canonical sequence as follows:
76-206: Missing.
Computationally mapped potential isoform sequencesi
There are 7 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketB4DLJ1 | B4DLJ1_HUMAN | Upstream stimulatory factor 2 | USF2 | 349 | Annotation score: | ||
M0QXT0 | M0QXT0_HUMAN | Upstream stimulatory factor 2 | USF2 | 253 | Annotation score: | ||
M0R1C6 | M0R1C6_HUMAN | Upstream stimulatory factor 2 | USF2 | 143 | Annotation score: | ||
M0QY46 | M0QY46_HUMAN | Upstream stimulatory factor 2 | USF2 | 170 | Annotation score: | ||
M0QYU7 | M0QYU7_HUMAN | Upstream stimulatory factor 2 | USF2 | 149 | Annotation score: | ||
V9GYR5 | V9GYR5_HUMAN | Upstream stimulatory factor 2 | USF2 | 112 | Annotation score: | ||
M0QZ39 | M0QZ39_HUMAN | Upstream stimulatory factor 2 | USF2 | 86 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 46 – 64 | QTAVA…GDHNI → GGGTSGGRGSGIQTRVQHV (PubMed:1450663).CuratedAdd BLAST | 19 | |
Sequence conflicti | 93 – 100 | GDTAGAVS → EFHSWRRH in M77476 (PubMed:1589769).Curated | 8 | |
Sequence conflicti | 122 | A → V in M77476 (PubMed:1589769).Curated | 1 | |
Sequence conflicti | 308 | Q → R in CAG38742 (Ref. 4) Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_047804 | 76 – 206 | Missing in isoform USF2c. 1 PublicationAdd BLAST | 131 | |
Alternative sequenceiVSP_002164 | 77 – 143 | Missing in isoform USF2B. 1 PublicationAdd BLAST | 67 | |
Alternative sequenceiVSP_002165 | 275 – 282 | Missing in isoform USF2A-delta-H. 1 Publication | 8 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X90824 mRNA Translation: CAA62339.1 X90825 mRNA Translation: CAA62340.1 X90826 mRNA Translation: CAA62341.1 Y07661 Genomic DNA Translation: CAA68942.1 AY147880 mRNA Translation: AAN63092.1 CR536504 mRNA Translation: CAG38742.1 AD000684 Genomic DNA Translation: AAB51179.1 BC049821 mRNA Translation: AAH49821.1 S50537 mRNA Translation: AAB24368.1 M77476 mRNA No translation available. |
CCDSi | CCDS12452.1 [Q15853-1] CCDS12453.1 [Q15853-3] CCDS82329.1 [Q15853-4] |
PIRi | I54074 |
RefSeqi | NP_001308079.1, NM_001321150.1 [Q15853-4] NP_003358.1, NM_003367.3 [Q15853-1] NP_997174.1, NM_207291.2 [Q15853-3] |
Genome annotation databases
Ensembli | ENST00000222305; ENSP00000222305; ENSG00000105698 [Q15853-1] ENST00000343550; ENSP00000340633; ENSG00000105698 [Q15853-3] ENST00000379134; ENSP00000368429; ENSG00000105698 [Q15853-4] ENST00000595068; ENSP00000471099; ENSG00000105698 [Q15853-2] |
GeneIDi | 7392 |
KEGGi | hsa:7392 |
UCSCi | uc002nyq.2, human [Q15853-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X90824 mRNA Translation: CAA62339.1 X90825 mRNA Translation: CAA62340.1 X90826 mRNA Translation: CAA62341.1 Y07661 Genomic DNA Translation: CAA68942.1 AY147880 mRNA Translation: AAN63092.1 CR536504 mRNA Translation: CAG38742.1 AD000684 Genomic DNA Translation: AAB51179.1 BC049821 mRNA Translation: AAH49821.1 S50537 mRNA Translation: AAB24368.1 M77476 mRNA No translation available. |
CCDSi | CCDS12452.1 [Q15853-1] CCDS12453.1 [Q15853-3] CCDS82329.1 [Q15853-4] |
PIRi | I54074 |
RefSeqi | NP_001308079.1, NM_001321150.1 [Q15853-4] NP_003358.1, NM_003367.3 [Q15853-1] NP_997174.1, NM_207291.2 [Q15853-3] |
3D structure databases
SMRi | Q15853 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 113238, 35 interactors |
ComplexPortali | CPX-3079, USF1-USF2 upstream stimulatory factor complex CPX-3083, USF2 upstream stimulatory factor complex |
CORUMi | Q15853 |
IntActi | Q15853, 19 interactors |
MINTi | Q15853 |
STRINGi | 9606.ENSP00000222305 |
PTM databases
iPTMneti | Q15853 |
MetOSitei | Q15853 |
PhosphoSitePlusi | Q15853 |
Genetic variation databases
BioMutai | USF2 |
DMDMi | 2833271 |
Proteomic databases
EPDi | Q15853 |
jPOSTi | Q15853 |
MassIVEi | Q15853 |
MaxQBi | Q15853 |
PaxDbi | Q15853 |
PeptideAtlasi | Q15853 |
PRIDEi | Q15853 |
ProteomicsDBi | 60794 [Q15853-1] 60795 [Q15853-2] 60796 [Q15853-3] 67853 |
Protocols and materials databases
Antibodypediai | 15859, 357 antibodies |
Genome annotation databases
Ensembli | ENST00000222305; ENSP00000222305; ENSG00000105698 [Q15853-1] ENST00000343550; ENSP00000340633; ENSG00000105698 [Q15853-3] ENST00000379134; ENSP00000368429; ENSG00000105698 [Q15853-4] ENST00000595068; ENSP00000471099; ENSG00000105698 [Q15853-2] |
GeneIDi | 7392 |
KEGGi | hsa:7392 |
UCSCi | uc002nyq.2, human [Q15853-1] |
Organism-specific databases
CTDi | 7392 |
DisGeNETi | 7392 |
GeneCardsi | USF2 |
HGNCi | HGNC:12594, USF2 |
HPAi | ENSG00000105698, Low tissue specificity |
MIMi | 600390, gene |
neXtProti | NX_Q15853 |
OpenTargetsi | ENSG00000105698 |
PharmGKBi | PA37224 |
VEuPathDBi | HostDB:ENSG00000105698.15 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG1318, Eukaryota |
GeneTreei | ENSGT00940000160704 |
HOGENOMi | CLU_070485_2_0_1 |
InParanoidi | Q15853 |
OrthoDBi | 1345445at2759 |
PhylomeDBi | Q15853 |
TreeFami | TF323338 |
Enzyme and pathway databases
PathwayCommonsi | Q15853 |
Reactomei | R-HSA-9018519, Estrogen-dependent gene expression |
SignaLinki | Q15853 |
SIGNORi | Q15853 |
Miscellaneous databases
BioGRID-ORCSi | 7392, 172 hits in 897 CRISPR screens |
ChiTaRSi | USF2, human |
GeneWikii | USF2 |
GenomeRNAii | 7392 |
Pharosi | Q15853, Tbio |
PROi | PR:Q15853 |
RNActi | Q15853, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000105698, Expressed in frontal cortex and 247 other tissues |
ExpressionAtlasi | Q15853, baseline and differential |
Genevisiblei | Q15853, HS |
Family and domain databases
CDDi | cd00083, HLH, 1 hit |
Gene3Di | 4.10.280.10, 1 hit |
InterProi | View protein in InterPro IPR011598, bHLH_dom IPR036638, HLH_DNA-bd_sf |
Pfami | View protein in Pfam PF00010, HLH, 1 hit |
SMARTi | View protein in SMART SM00353, HLH, 1 hit |
SUPFAMi | SSF47459, SSF47459, 1 hit |
PROSITEi | View protein in PROSITE PS50888, BHLH, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | USF2_HUMAN | |
Accessioni | Q15853Primary (citable) accession number: Q15853 Secondary accession number(s): O00671 Q6YI47 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | November 1, 1996 | |
Last modified: | February 10, 2021 | |
This is version 186 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human chromosome 19
Human chromosome 19: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot