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Protein

NEDD8

Gene

NEDD8

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Ubiquitin-like protein which plays an important role in cell cycle control and embryogenesis. Covalent attachment to its substrates requires prior activation by the E1 complex UBE1C-APPBP1 and linkage to the E2 enzyme UBE2M. Attachment of NEDD8 to cullins activates their associated E3 ubiquitin ligase activity, and thus promotes polyubiquitination and proteasomal degradation of cyclins and other regulatory proteins.3 Publications

GO - Molecular functioni

  • ubiquitin protein ligase binding Source: UniProtKB

GO - Biological processi

  • anatomical structure morphogenesis Source: ProtInc
  • cellular iron ion homeostasis Source: Reactome
  • cellular protein modification process Source: ProtInc
  • post-translational protein modification Source: Reactome
  • protein deubiquitination Source: Reactome
  • protein localization Source: Ensembl
  • protein neddylation Source: MGI
  • proteolysis Source: ProtInc
  • regulation of transcription by RNA polymerase II Source: Ensembl
  • response to organic cyclic compound Source: Ensembl
  • ubiquitin-dependent protein catabolic process Source: ProtInc

Keywordsi

Biological processUbl conjugation pathway

Enzyme and pathway databases

ReactomeiR-HSA-2173789 TGF-beta receptor signaling activates SMADs
R-HSA-5689603 UCH proteinases
R-HSA-8856825 Cargo recognition for clathrin-mediated endocytosis
R-HSA-8951664 Neddylation
R-HSA-917937 Iron uptake and transport

Protein family/group databases

MoonDBiQ15843 Predicted

Names & Taxonomyi

Protein namesi
Recommended name:
NEDD8
Alternative name(s):
Neddylin
Neural precursor cell expressed developmentally down-regulated protein 8
Short name:
NEDD-8
Ubiquitin-like protein Nedd8
Gene namesi
Name:NEDD8
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 14

Organism-specific databases

EuPathDBiHostDB:ENSG00000129559.12
HGNCiHGNC:7732 NEDD8
MIMi603171 gene
neXtProtiNX_Q15843

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi72A → R: Prevents adenylation by UBE1C. 1 Publication1

Organism-specific databases

DisGeNETi4738
OpenTargetsiENSG00000129559
PharmGKBiPA31537

Polymorphism and mutation databases

BioMutaiNEDD8
DMDMi2833270

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000427671 – 76NEDD8Add BLAST76
PropeptideiPRO_000004276877 – 815

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei48N6-acetyllysineBy similarity1
Cross-linki76Glycyl lysine isopeptide (Gly-Lys) (interchain with K-? in acceptor proteins)

Post-translational modificationi

Cleavage of precursor form by UCHL3 or SENP8 is necessary for function.

Keywords - PTMi

Acetylation, Isopeptide bond

Proteomic databases

EPDiQ15843
MaxQBiQ15843
PaxDbiQ15843
PeptideAtlasiQ15843
PRIDEiQ15843
ProteomicsDBi12687
60788
TopDownProteomicsiQ15843

PTM databases

iPTMnetiQ15843
PhosphoSitePlusiQ15843

Miscellaneous databases

PMAP-CutDBiQ15843

Expressioni

Tissue specificityi

Highly expressed in heart, skeletal muscle, spleen, thymus, prostate, testis, ovary, colon and leukocytes.2 Publications

Gene expression databases

BgeeiENSG00000129559
CleanExiHS_NEDD8
ExpressionAtlasiQ15843 baseline and differential
GenevisibleiQ15843 HS

Organism-specific databases

HPAiCAB004082
HPA027583
HPA060254

Interactioni

Subunit structurei

Directly interacts with NUB1 and AHR. Covalently attached to cullins and p53.8 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei8Interaction with UBE1C1
Sitei44Interaction with UBE1C1

Binary interactionsi

Show more details

GO - Molecular functioni

  • ubiquitin protein ligase binding Source: UniProtKB

Protein-protein interaction databases

BioGridi110815, 264 interactors
CORUMiQ15843
DIPiDIP-29266N
IntActiQ15843, 104 interactors
MINTiQ15843
STRINGi9606.ENSP00000250495

Structurei

Secondary structure

181
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi2 – 6Combined sources5
Beta strandi8 – 10Combined sources3
Beta strandi12 – 16Combined sources5
Helixi23 – 34Combined sources12
Helixi38 – 40Combined sources3
Beta strandi41 – 45Combined sources5
Beta strandi52 – 55Combined sources4
Helixi56 – 59Combined sources4
Beta strandi66 – 71Combined sources6
Beta strandi73 – 75Combined sources3

3D structure databases

ProteinModelPortaliQ15843
SMRiQ15843
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ15843

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni70 – 72Interaction with UBE1C3

Sequence similaritiesi

Belongs to the ubiquitin family.Curated

Phylogenomic databases

eggNOGiKOG0005 Eukaryota
COG5272 LUCA
GeneTreeiENSGT00900000141147
HOGENOMiHOG000233942
HOVERGENiHBG000057
InParanoidiQ15843
KOiK12158
OMAiGKQMTDD
OrthoDBiEOG091G178I
PhylomeDBiQ15843
TreeFamiTF300072

Family and domain databases

CDDicd01806 Nedd8, 1 hit
InterProiView protein in InterPro
IPR038738 Nedd8-like
IPR019956 Ubiquitin
IPR029071 Ubiquitin-like_domsf
IPR019954 Ubiquitin_CS
IPR000626 Ubiquitin_dom
PfamiView protein in Pfam
PF00240 ubiquitin, 1 hit
PRINTSiPR00348 UBIQUITIN
SMARTiView protein in SMART
SM00213 UBQ, 1 hit
SUPFAMiSSF54236 SSF54236, 1 hit
PROSITEiView protein in PROSITE
PS00299 UBIQUITIN_1, 1 hit
PS50053 UBIQUITIN_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q15843-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLIKVKTLTG KEIEIDIEPT DKVERIKERV EEKEGIPPQQ QRLIYSGKQM
60 70 80
NDEKTAADYK ILGGSVLHLV LALRGGGGLR Q
Length:81
Mass (Da):9,072
Last modified:November 1, 1996 - v1
Checksum:iDC2FE102BE4725D2
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti15I → V in CAG28590 (Ref. 2) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D23662 mRNA Translation: BAA04889.1
CR407662 mRNA Translation: CAG28590.1
BC104200 mRNA Translation: AAI04201.1
BC104201 mRNA Translation: AAI04202.1
BC104664 mRNA Translation: AAI04665.1
CCDSiCCDS9621.1
RefSeqiNP_006147.1, NM_006156.2
UniGeneiHs.531064

Genome annotation databases

EnsembliENST00000250495; ENSP00000250495; ENSG00000129559
ENST00000643069; ENSP00000496420; ENSG00000285246
GeneIDi4738
KEGGihsa:4738
UCSCiuc001wnn.3 human

Similar proteinsi

Entry informationi

Entry nameiNEDD8_HUMAN
AccessioniPrimary (citable) accession number: Q15843
Secondary accession number(s): Q3SXN8, Q6LES6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: July 18, 2018
This is version 169 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 14
    Human chromosome 14: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

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