UniProtKB - Q15746 (MYLK_HUMAN)
Myosin light chain kinase, smooth muscle
MYLK
Functioni
Miscellaneous
Catalytic activityi
Cofactori
Protein has several cofactor binding sites:Activity regulationi
Kineticsi
- KM=6.5 µM for MLC (isoform 1 at 22 degrees Celsius)1 Publication
- KM=7.2 µM for MLC (isoform 2 at 22 degrees Celsius)1 Publication
- KM=9.3 µM for MLC1 Publication
- Vmax=11.9 µmol/min/mg enzyme (isoform 1 at 22 degrees Celsius)1 Publication
- Vmax=10.9 µmol/min/mg enzyme (isoform 1 at 22 degrees Celsius)1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 1493 | ATPPROSITE-ProRule annotation | 1 | |
Active sitei | 1585 | Proton acceptorPROSITE-ProRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 1470 – 1478 | ATPPROSITE-ProRule annotation | 9 |
GO - Molecular functioni
- actin binding Source: UniProtKB-KW
- ATP binding Source: UniProtKB-KW
- calmodulin binding Source: UniProtKB-KW
- metal ion binding Source: UniProtKB-KW
- myosin light chain kinase activity Source: UniProtKB
- protein kinase activity Source: GO_Central
GO - Biological processi
- aorta smooth muscle tissue morphogenesis Source: BHF-UCL
- bleb assembly Source: UniProtKB
- cellular hypotonic response Source: UniProtKB
- muscle contraction Source: Reactome
- positive regulation of calcium ion transport Source: UniProtKB
- positive regulation of cell migration Source: UniProtKB
- positive regulation of wound healing Source: UniProtKB
- protein phosphorylation Source: ProtInc
- smooth muscle contraction Source: UniProtKB
- tonic smooth muscle contraction Source: UniProtKB
Keywordsi
Molecular function | Actin-binding, Calmodulin-binding, Kinase, Serine/threonine-protein kinase, Transferase |
Ligand | ATP-binding, Calcium, Magnesium, Metal-binding, Nucleotide-binding |
Enzyme and pathway databases
BRENDAi | 2.7.11.18, 2681 |
PathwayCommonsi | Q15746 |
Reactomei | R-HSA-445355, Smooth Muscle Contraction R-HSA-5627123, RHO GTPases activate PAKs |
SABIO-RKi | Q15746 |
SignaLinki | Q15746 |
SIGNORi | Q15746 |
Names & Taxonomyi
Protein namesi | Recommended name: Myosin light chain kinase, smooth muscleCurated (EC:2.7.11.18)Short name: MLCK Short name: smMLCK Alternative name(s): Kinase-related protein Short name: KRP Telokin Cleaved into the following chain: |
Gene namesi | |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:7590, MYLK |
MIMi | 600922, gene |
neXtProti | NX_Q15746 |
VEuPathDBi | HostDB:ENSG00000065534.18 |
Subcellular locationi
Cytoskeleton
- stress fiber 1 Publication
Other locations
- Cytoplasm 1 Publication
- lamellipodium 1 Publication
- Cleavage furrow 1 Publication
Note: Localized to stress fibers during interphase and to the cleavage furrow during mitosis.1 Publication
Cytoskeleton
- actin cytoskeleton Source: HPA
- stress fiber Source: UniProtKB
Cytosol
- cytosol Source: Reactome
Plasma Membrane
- cleavage furrow Source: UniProtKB
- plasma membrane Source: HPA
Other locations
- cytoplasm Source: UniProtKB
- lamellipodium Source: UniProtKB
Keywords - Cellular componenti
Cell projection, Cytoplasm, CytoskeletonPathology & Biotechi
Involvement in diseasei
Aortic aneurysm, familial thoracic 7 (AAT7)3 Publications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_065574 | 1213 | V → M in AAT7; unknown pathological significance. 1 PublicationCorresponds to variant dbSNP:rs368390254EnsemblClinVar. | 1 | |
Natural variantiVAR_083423 | 1458 – 1914 | Missing in AAT7; unknown pathological significance. 1 PublicationAdd BLAST | 457 | |
Natural variantiVAR_083424 | 1487 – 1914 | Missing in AAT7; unknown pathological significance. 1 PublicationAdd BLAST | 428 | |
Natural variantiVAR_083425 | 1491 | A → S in AAT7; decreases kinase activity. 1 Publication | 1 | |
Natural variantiVAR_065576 | 1754 | A → T in AAT7; 4-fold reduced affinity for calmodulin; decreased kinase activity compared to wild-type protein. 1 Publication | 1 | |
Natural variantiVAR_065577 | 1759 | S → P in AAT7; 7-fold reduced affinity for calmodulin; 6-fold decreased Vmax. 1 PublicationCorresponds to variant dbSNP:rs387906781EnsemblClinVar. | 1 |
Megacystis-microcolon-intestinal hypoperistalsis syndrome (MMIHS)1 Publication
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 608 | K → A: Loss of acetylation and no kinase activity repression by NAA10/ARD1. 1 Publication | 1 |
Keywords - Diseasei
Aortic aneurysm, Disease variantOrganism-specific databases
DisGeNETi | 4638 |
GeneReviewsi | MYLK |
MalaCardsi | MYLK |
MIMi | 249210, phenotype 613780, phenotype |
OpenTargetsi | ENSG00000065534 |
Orphaneti | 91387, Familial thoracic aortic aneurysm and aortic dissection 2241, Megacystis-microcolon-intestinal hypoperistalsis syndrome |
PharmGKBi | PA31388 |
Miscellaneous databases
Pharosi | Q15746, Tchem |
Chemistry databases
ChEMBLi | CHEMBL2428 |
DrugBanki | DB12010, Fostamatinib |
DrugCentrali | Q15746 |
GuidetoPHARMACOLOGYi | 1552 |
Genetic variation databases
BioMutai | MYLK |
DMDMi | 300669714 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length | |
---|---|---|---|---|---|
ChainiPRO_0000024354 | 1 – 1914 | Myosin light chain kinase, smooth muscleAdd BLAST | 1914 | ||
ChainiPRO_0000403731 | 1 – 1910 | Myosin light chain kinase, smooth muscle, deglutamylated formBy similarityAdd BLAST | 1910 | ||
Isoform 6 (identifier: Q15746-8) | |||||
Initiator methioninei | RemovedCombined sources | ||||
Isoform 8 (identifier: Q15746-10) | |||||
Initiator methioninei | RemovedCombined sources |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length | |
---|---|---|---|---|---|
Disulfide bondi | 182 ↔ 233 | PROSITE-ProRule annotation | |||
Modified residuei | 231 | Phosphotyrosine; by ABL11 Publication | 1 | ||
Modified residuei | 305 | PhosphoserineCombined sources | 1 | ||
Modified residuei | 343 | PhosphoserineBy similarity | 1 | ||
Modified residuei | 365 | PhosphoserineBy similarity | 1 | ||
Disulfide bondi | 435 ↔ 487 | PROSITE-ProRule annotation | |||
Modified residuei | 464 | Phosphotyrosine; by ABL1 and SRC3 Publications | 1 | ||
Modified residuei | 471 | Phosphotyrosine; by SRC2 Publications | 1 | ||
Disulfide bondi | 535 ↔ 583 | PROSITE-ProRule annotation | |||
Modified residuei | 556 | Phosphotyrosine; by ABL11 Publication | 1 | ||
Modified residuei | 608 | N6-acetyllysine1 Publication | 1 | ||
Modified residuei | 611 | Phosphotyrosine; by ABL11 Publication | 1 | ||
Disulfide bondi | 742 ↔ 805 | PROSITE-ProRule annotation | |||
Modified residuei | 792 | Phosphotyrosine; by ABL11 Publication | 1 | ||
Modified residuei | 846 | Phosphotyrosine; by ABL11 Publication | 1 | ||
Modified residuei | 947 | PhosphoserineBy similarity | 1 | ||
Disulfide bondi | 1119 ↔ 1170 | PROSITE-ProRule annotation | |||
Modified residuei | 1438 | PhosphoserineCombined sources | 1 | ||
Modified residuei | 1449 | Phosphotyrosine; by ABL11 Publication | 1 | ||
Modified residuei | 1575 | Phosphotyrosine; by ABL11 Publication | 1 | ||
Modified residuei | 1635 | Phosphotyrosine; by ABL11 Publication | 1 | ||
Modified residuei | 1759 | PhosphoserineBy similarity | 1 | ||
Modified residuei | 1760 | PhosphoserineBy similarity | 1 | ||
Modified residuei | 1772 | PhosphoserineCombined sources | 1 | ||
Modified residuei | 1773 | PhosphoserineBy similarity | 1 | ||
Modified residuei | 1776 | PhosphoserineCombined sources | 1 | ||
Modified residuei | 1778 | PhosphothreonineBy similarity | 1 | ||
Modified residuei | 1779 | PhosphoserineCombined sources | 1 | ||
Disulfide bondi | 1830 ↔ 1882 | PROSITE-ProRule annotation | |||
Isoform 6 (identifier: Q15746-8) | |||||
Modified residuei | 2 | N-acetylalanineCombined sources | 1 | ||
Isoform 8 (identifier: Q15746-10) | |||||
Modified residuei | 2 | N-acetylalanineCombined sources | 1 |
Post-translational modificationi
Keywords - PTMi
Acetylation, Disulfide bond, PhosphoproteinProteomic databases
EPDi | Q15746 |
jPOSTi | Q15746 |
MassIVEi | Q15746 |
MaxQBi | Q15746 |
PaxDbi | Q15746 |
PeptideAtlasi | Q15746 |
PRIDEi | Q15746 |
ProteomicsDBi | 5180 60733 [Q15746-1] 60734 [Q15746-2] 60735 [Q15746-3] 60736 [Q15746-4] 60737 [Q15746-5] 60738 [Q15746-6] 60739 [Q15746-7] 60740 [Q15746-8] |
PTM databases
iPTMneti | Q15746 |
MetOSitei | Q15746 |
PhosphoSitePlusi | Q15746 |
Expressioni
Tissue specificityi
Inductioni
Gene expression databases
Bgeei | ENSG00000065534, Expressed in cauda epididymis and 252 other tissues |
ExpressionAtlasi | Q15746, baseline and differential |
Genevisiblei | Q15746, HS |
Organism-specific databases
HPAi | ENSG00000065534, Tissue enhanced (smooth) |
Interactioni
Subunit structurei
All isoforms including Telokin bind calmodulin.
Interacts with SVIL (By similarity).
Interacts with CTTN; this interaction is reduced during thrombin-induced endothelial cell (EC) contraction but is promoted by the barrier-protective agonist sphingosine 1-phosphate (S1P) within lamellipodia. A complex made of ABL1, CTTN and MYLK regulates cortical actin-based cytoskeletal rearrangement critical to sphingosine 1-phosphate (S1P)-mediated endothelial cell (EC) barrier enhancement. Binds to NAA10/ARD1 and PTK2B/PYK2.
By similarity6 PublicationsBinary interactionsi
Hide detailsQ15746
With | #Exp. | IntAct |
---|---|---|
GOPC [Q9HD26] | 3 | EBI-968482,EBI-349832 |
NCK1 [P16333] | 2 | EBI-968482,EBI-389883 |
Isoform 5 [Q15746-7]
With | #Exp. | IntAct |
---|---|---|
APPBP2 [Q92624] | 3 | EBI-12189939,EBI-743771 |
GOPC - isoform 2 [Q9HD26-2] | 5 | EBI-12189939,EBI-11102276 |
GO - Molecular functioni
- actin binding Source: UniProtKB-KW
- calmodulin binding Source: UniProtKB-KW
Protein-protein interaction databases
BioGRIDi | 110722, 23 interactors |
CORUMi | Q15746 |
IntActi | Q15746, 28 interactors |
MINTi | Q15746 |
STRINGi | 9606.ENSP00000353452 |
Chemistry databases
BindingDBi | Q15746 |
Miscellaneous databases
RNActi | Q15746, protein |
Structurei
Secondary structure
3D structure databases
SMRi | Q15746 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q15746 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 33 – 122 | Ig-like C2-type 1Add BLAST | 90 | |
Domaini | 161 – 249 | Ig-like C2-type 2Add BLAST | 89 | |
Domaini | 414 – 503 | Ig-like C2-type 3Add BLAST | 90 | |
Domaini | 514 – 599 | Ig-like C2-type 4Add BLAST | 86 | |
Domaini | 620 – 711 | Ig-like C2-type 5Add BLAST | 92 | |
Domaini | 721 – 821 | Ig-like C2-type 6Add BLAST | 101 | |
Repeati | 868 – 895 | 1-1Add BLAST | 28 | |
Repeati | 896 – 923 | 1-2Add BLAST | 28 | |
Repeati | 924 – 951 | 1-3Add BLAST | 28 | |
Repeati | 952 – 979 | 1-4Add BLAST | 28 | |
Repeati | 980 – 998 | 1-5; truncatedAdd BLAST | 19 | |
Repeati | 999 – 1003 | 2-1; truncated | 5 | |
Repeati | 1004 – 1015 | 2-2Add BLAST | 12 | |
Repeati | 1016 – 1027 | 2-3Add BLAST | 12 | |
Repeati | 1028 – 1039 | 2-4Add BLAST | 12 | |
Repeati | 1040 – 1051 | 2-5Add BLAST | 12 | |
Repeati | 1052 – 1063 | 2-6Add BLAST | 12 | |
Domaini | 1098 – 1186 | Ig-like C2-type 7Add BLAST | 89 | |
Domaini | 1238 – 1326 | Ig-like C2-type 8Add BLAST | 89 | |
Domaini | 1334 – 1426 | Fibronectin type-IIIPROSITE-ProRule annotationAdd BLAST | 93 | |
Domaini | 1464 – 1719 | Protein kinasePROSITE-ProRule annotationAdd BLAST | 256 | |
Domaini | 1809 – 1898 | Ig-like C2-type 9Add BLAST | 90 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 868 – 998 | 5 X 28 AA approximate tandem repeatsAdd BLAST | 131 | |
Regioni | 923 – 963 | Actin-binding (calcium/calmodulin-sensitive)By similarityAdd BLAST | 41 | |
Regioni | 948 – 963 | Calmodulin-bindingBy similarityAdd BLAST | 16 | |
Regioni | 999 – 1063 | 6 X 12 AA approximate tandem repeatsAdd BLAST | 65 | |
Regioni | 1061 – 1460 | Actin-binding (calcium/calmodulin-insensitive)By similarityAdd BLAST | 400 | |
Regioni | 1711 – 1774 | Calmodulin-bindingAdd BLAST | 64 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 1906 – 1914 | Poly-Glu | 9 |
Sequence similaritiesi
Keywords - Domaini
Immunoglobulin domain, RepeatPhylogenomic databases
eggNOGi | KOG0613, Eukaryota |
GeneTreei | ENSGT00940000157879 |
HOGENOMi | CLU_000288_76_2_1 |
InParanoidi | Q15746 |
OMAi | DVNCKKG |
OrthoDBi | 330091at2759 |
PhylomeDBi | Q15746 |
TreeFami | TF314166 |
Family and domain databases
CDDi | cd00063, FN3, 1 hit cd14191, STKc_MLCK1, 1 hit |
Gene3Di | 2.60.40.10, 10 hits |
InterProi | View protein in InterPro IPR003961, FN3_dom IPR036116, FN3_sf IPR007110, Ig-like_dom IPR036179, Ig-like_dom_sf IPR013783, Ig-like_fold IPR013098, Ig_I-set IPR003599, Ig_sub IPR003598, Ig_sub2 IPR011009, Kinase-like_dom_sf IPR015725, MLCK1_kinase_dom IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR008271, Ser/Thr_kinase_AS |
Pfami | View protein in Pfam PF00041, fn3, 1 hit PF07679, I-set, 9 hits PF00069, Pkinase, 1 hit |
SMARTi | View protein in SMART SM00060, FN3, 1 hit SM00409, IG, 9 hits SM00408, IGc2, 9 hits SM00220, S_TKc, 1 hit |
SUPFAMi | SSF48726, SSF48726, 9 hits SSF49265, SSF49265, 1 hit SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS50853, FN3, 1 hit PS50835, IG_LIKE, 9 hits PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit |
s (11+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 11 produced by isoformsialternative promoter usage and alternative splicing. AlignAdd to basketThis entry has 11 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MGDVKLVASS HISKTSLSVD PSRVDSMPLT EAPAFILPPR NLCIKEGATA
60 70 80 90 100
KFEGRVRGYP EPQVTWHRNG QPITSGGRFL LDCGIRGTFS LVIHAVHEED
110 120 130 140 150
RGKYTCEATN GSGARQVTVE LTVEGSFAKQ LGQPVVSKTL GDRFSAPAVE
160 170 180 190 200
TRPSIWGECP PKFATKLGRV VVKEGQMGRF SCKITGRPQP QVTWLKGNVP
210 220 230 240 250
LQPSARVSVS EKNGMQVLEI HGVNQDDVGV YTCLVVNGSG KASMSAELSI
260 270 280 290 300
QGLDSANRSF VRETKATNSD VRKEVTNVIS KESKLDSLEA AAKSKNCSSP
310 320 330 340 350
QRGGSPPWAA NSQPQPPRES KLESCKDSPR TAPQTPVLQK TSSSITLQAA
360 370 380 390 400
RVQPEPRAPG LGVLSPSGEE RKRPAPPRPA TFPTRQPGLG SQDVVSKAAN
410 420 430 440 450
RRIPMEGQRD SAFPKFESKP QSQEVKENQT VKFRCEVSGI PKPEVAWFLE
460 470 480 490 500
GTPVRRQEGS IEVYEDAGSH YLCLLKARTR DSGTYSCTAS NAQGQLSCSW
510 520 530 540 550
TLQVERLAVM EVAPSFSSVL KDCAVIEGQD FVLQCSVRGT PVPRITWLLN
560 570 580 590 600
GQPIQYARST CEAGVAELHI QDALPEDHGT YTCLAENALG QVSCSAWVTV
610 620 630 640 650
HEKKSSRKSE YLLPVAPSKP TAPIFLQGLS DLKVMDGSQV TMTVQVSGNP
660 670 680 690 700
PPEVIWLHNG NEIQESEDFH FEQRGTQHSL CIQEVFPEDT GTYTCEAWNS
710 720 730 740 750
AGEVRTQAVL TVQEPHDGTQ PWFISKPRSV TASLGQSVLI SCAIAGDPFP
760 770 780 790 800
TVHWLRDGKA LCKDTGHFEV LQNEDVFTLV LKKVQPWHAG QYEILLKNRV
810 820 830 840 850
GECSCQVSLM LQNSSARALP RGREPASCED LCGGGVGADG GGSDRYGSLR
860 870 880 890 900
PGWPARGQGW LEEEDGEDVR GVLKRRVETR QHTEEAIRQQ EVEQLDFRDL
910 920 930 940 950
LGKKVSTKTL SEDDLKEIPA EQMDFRANLQ RQVKPKTVSE EERKVHSPQQ
960 970 980 990 1000
VDFRSVLAKK GTSKTPVPEK VPPPKPATPD FRSVLGGKKK LPAENGSSSA
1010 1020 1030 1040 1050
ETLNAKAVES SKPLSNAQPS GPLKPVGNAK PAETLKPMGN AKPAETLKPM
1060 1070 1080 1090 1100
GNAKPDENLK SASKEELKKD VKNDVNCKRG HAGTTDNEKR SESQGTAPAF
1110 1120 1130 1140 1150
KQKLQDVHVA EGKKLLLQCQ VSSDPPATII WTLNGKTLKT TKFIILSQEG
1160 1170 1180 1190 1200
SLCSVSIEKA LPEDRGLYKC VAKNDAGQAE CSCQVTVDDA PASENTKAPE
1210 1220 1230 1240 1250
MKSRRPKSSL PPVLGTESDA TVKKKPAPKT PPKAAMPPQI IQFPEDQKVR
1260 1270 1280 1290 1300
AGESVELFGK VTGTQPITCT WMKFRKQIQE SEHMKVENSE NGSKLTILAA
1310 1320 1330 1340 1350
RQEHCGCYTL LVENKLGSRQ AQVNLTVVDK PDPPAGTPCA SDIRSSSLTL
1360 1370 1380 1390 1400
SWYGSSYDGG SAVQSYSIEI WDSANKTWKE LATCRSTSFN VQDLLPDHEY
1410 1420 1430 1440 1450
KFRVRAINVY GTSEPSQESE LTTVGEKPEE PKDEVEVSDD DEKEPEVDYR
1460 1470 1480 1490 1500
TVTINTEQKV SDFYDIEERL GSGKFGQVFR LVEKKTRKVW AGKFFKAYSA
1510 1520 1530 1540 1550
KEKENIRQEI SIMNCLHHPK LVQCVDAFEE KANIVMVLEI VSGGELFERI
1560 1570 1580 1590 1600
IDEDFELTER ECIKYMRQIS EGVEYIHKQG IVHLDLKPEN IMCVNKTGTR
1610 1620 1630 1640 1650
IKLIDFGLAR RLENAGSLKV LFGTPEFVAP EVINYEPIGY ATDMWSIGVI
1660 1670 1680 1690 1700
CYILVSGLSP FMGDNDNETL ANVTSATWDF DDEAFDEISD DAKDFISNLL
1710 1720 1730 1740 1750
KKDMKNRLDC TQCLQHPWLM KDTKNMEAKK LSKDRMKKYM ARRKWQKTGN
1760 1770 1780 1790 1800
AVRAIGRLSS MAMISGLSGR KSSTGSPTSP LNAEKLESEE DVSQAFLEAV
1810 1820 1830 1840 1850
AEEKPHVKPY FSKTIRDLEV VEGSAARFDC KIEGYPDPEV VWFKDDQSIR
1860 1870 1880 1890 1900
ESRHFQIDYD EDGNCSLIIS DVCGDDDAKY TCKAVNSLGE ATCTAELIVE
1910
TMEEGEGEGE EEEE
The sequence of this isoform differs from the canonical sequence as follows:
1655-1705: Missing.
The sequence of this isoform differs from the canonical sequence as follows:
437-506: VSGIPKPEVAWFLEGTPVRRQEGSIEVYEDAGSHYLCLLKARTRDSGTYSCTASNAQGQLSCSWTLQVER → G
1655-1705: Missing.
The sequence of this isoform differs from the canonical sequence as follows:
1790-1790: Missing.
The sequence of this isoform differs from the canonical sequence as follows:
1-922: Missing.
The sequence of this isoform differs from the canonical sequence as follows:
1-1760: Missing.
The sequence of this isoform differs from the canonical sequence as follows:
1-1760: Missing.
1790-1790: Missing.
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketD6R9C2 | D6R9C2_HUMAN | Myosin light chain kinase, smooth m... | MYLK | 229 | Annotation score: | ||
F8WBL7 | F8WBL7_HUMAN | Myosin light chain kinase, smooth m... | MYLK | 70 | Annotation score: |
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 147 | P → S in AAC18423 (PubMed:9160829).Curated | 1 | |
Sequence conflicti | 147 | P → S in AAD15922 (PubMed:10198165).Curated | 1 | |
Sequence conflicti | 147 | P → S in AAD15923 (PubMed:10198165).Curated | 1 | |
Sequence conflicti | 147 | P → S in AAR29062 (PubMed:15507455).Curated | 1 | |
Sequence conflicti | 147 | P → S in AAQ02673 (PubMed:15020676).Curated | 1 | |
Sequence conflicti | 147 | P → S in EAW79438 (Ref. 11) Curated | 1 | |
Sequence conflicti | 147 | P → S in EAW79440 (Ref. 11) Curated | 1 | |
Sequence conflicti | 466 | D → N in AAR29062 (PubMed:15507455).Curated | 1 | |
Sequence conflicti | 496 | L → V in AAC18423 (PubMed:9160829).Curated | 1 | |
Sequence conflicti | 496 | L → V in AAD15922 (PubMed:10198165).Curated | 1 | |
Sequence conflicti | 496 | L → V in AAR29062 (PubMed:15507455).Curated | 1 | |
Sequence conflicti | 496 | L → V in AAQ02673 (PubMed:15020676).Curated | 1 | |
Sequence conflicti | 681 | C → W in AAC18423 (PubMed:9160829).Curated | 1 | |
Sequence conflicti | 681 | C → W in AAD15921 (PubMed:10198165).Curated | 1 | |
Sequence conflicti | 681 | C → W in AAD15922 (PubMed:10198165).Curated | 1 | |
Sequence conflicti | 681 | C → W in AAD15923 (PubMed:10198165).Curated | 1 | |
Sequence conflicti | 933 | V → M in CAA59685 (PubMed:8575746).Curated | 1 | |
Sequence conflicti | 963 | S → P in AAD15922 (PubMed:10198165).Curated | 1 | |
Sequence conflicti | 1022 | P → A in CAA59685 (PubMed:8575746).Curated | 1 | |
Sequence conflicti | 1048 – 1050 | KPM → EAH in CAA59685 (PubMed:8575746).Curated | 3 | |
Sequence conflicti | 1048 – 1050 | KPM → EAH in BAB21504 (Ref. 8) Curated | 3 | |
Sequence conflicti | 1162 | P → L in AAD15922 (PubMed:10198165).Curated | 1 | |
Sequence conflicti | 1162 | P → L in AAD15923 (PubMed:10198165).Curated | 1 | |
Sequence conflicti | 1210 | L → P in CAA59685 (PubMed:8575746).Curated | 1 | |
Sequence conflicti | 1280 | E → D in AAD15922 (PubMed:10198165).Curated | 1 | |
Sequence conflicti | 1280 | E → D in AAD15923 (PubMed:10198165).Curated | 1 | |
Sequence conflicti | 1284 | M → I in AAD15922 (PubMed:10198165).Curated | 1 | |
Sequence conflicti | 1284 | M → I in AAD15923 (PubMed:10198165).Curated | 1 | |
Sequence conflicti | 1284 | M → I in AAD15924 (PubMed:10198165).Curated | 1 | |
Sequence conflicti | 1300 | A → G in CAA59685 (PubMed:8575746).Curated | 1 | |
Sequence conflicti | 1316 | L → S in CAA59685 (PubMed:8575746).Curated | 1 | |
Sequence conflicti | 1326 | T → S in CAA59685 (PubMed:8575746).Curated | 1 | |
Sequence conflicti | 1478 | V → C in CAA59685 (PubMed:8575746).Curated | 1 | |
Sequence conflicti | 1511 | S → T in AAD15922 (PubMed:10198165).Curated | 1 | |
Sequence conflicti | 1511 | S → T in AAD15923 (PubMed:10198165).Curated | 1 | |
Sequence conflicti | 1518 | H → P in AAR29061 (PubMed:15507455).Curated | 1 | |
Sequence conflicti | 1518 | H → P in AAR29062 (PubMed:15507455).Curated | 1 | |
Sequence conflicti | 1563 | I → T in CAA59685 (PubMed:8575746).Curated | 1 | |
Sequence conflicti | 1609 | A → P in CAA59685 (PubMed:8575746).Curated | 1 | |
Sequence conflicti | 1634 | N → I in AAR29061 (PubMed:15507455).Curated | 1 | |
Sequence conflicti | 1634 | N → I in AAR29062 (PubMed:15507455).Curated | 1 | |
Sequence conflicti | 1639 – 1640 | GY → D in AAD15922 (PubMed:10198165).Curated | 2 | |
Sequence conflicti | 1639 – 1640 | GY → D in AAD15923 (PubMed:10198165).Curated | 2 | |
Sequence conflicti | 1639 – 1640 | GY → D in AAD15924 (PubMed:10198165).Curated | 2 | |
Sequence conflicti | 1639 | G → R in CAA59685 (PubMed:8575746).Curated | 1 | |
Sequence conflicti | 1648 | G → R in CAA59685 (PubMed:8575746).Curated | 1 | |
Sequence conflicti | 1658 – 1659 | LS → PF in CAA59685 (PubMed:8575746).Curated | 2 | |
Sequence conflicti | 1676 | A → P in AAR29061 (PubMed:15507455).Curated | 1 | |
Sequence conflicti | 1676 | A → P in AAR29062 (PubMed:15507455).Curated | 1 | |
Sequence conflicti | 1710 – 1711 | CT → LA in CAA59685 (PubMed:8575746).Curated | 2 | |
Sequence conflicti | 1897 | L → H in AAD15922 (PubMed:10198165).Curated | 1 | |
Sequence conflicti | 1897 | L → H in AAD15923 (PubMed:10198165).Curated | 1 | |
Sequence conflicti | 1897 | L → H in AAD15924 (PubMed:10198165).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_057106 | 21 | P → H. Corresponds to variant dbSNP:rs28497577EnsemblClinVar. | 1 | |
Natural variantiVAR_065570 | 128 | A → V1 PublicationCorresponds to variant dbSNP:rs143896146EnsemblClinVar. | 1 | |
Natural variantiVAR_065571 | 133 | Q → H1 PublicationCorresponds to variant dbSNP:rs140148380EnsemblClinVar. | 1 | |
Natural variantiVAR_065572 | 160 | P → R1 PublicationCorresponds to variant dbSNP:rs111256888EnsemblClinVar. | 1 | |
Natural variantiVAR_040847 | 261 | V → A1 PublicationCorresponds to variant dbSNP:rs3796164EnsemblClinVar. | 1 | |
Natural variantiVAR_040848 | 276 | T → A1 PublicationCorresponds to variant dbSNP:rs55846245Ensembl. | 1 | |
Natural variantiVAR_057107 | 336 | P → L. Corresponds to variant dbSNP:rs35912339EnsemblClinVar. | 1 | |
Natural variantiVAR_040849 | 378 | R → H1 PublicationCorresponds to variant dbSNP:rs56378658EnsemblClinVar. | 1 | |
Natural variantiVAR_040850 | 405 | M → V1 PublicationCorresponds to variant dbSNP:rs35436690EnsemblClinVar. | 1 | |
Natural variantiVAR_040851 | 443 | P → S1 PublicationCorresponds to variant dbSNP:rs35156360EnsemblClinVar. | 1 | |
Natural variantiVAR_040852 | 607 | R → G1 Publication | 1 | |
Natural variantiVAR_040853 | 652 | P → A1 PublicationCorresponds to variant dbSNP:rs750686734EnsemblClinVar. | 1 | |
Natural variantiVAR_040854 | 656 | W → C2 PublicationsCorresponds to variant dbSNP:rs138172035EnsemblClinVar. | 1 | |
Natural variantiVAR_040855 | 692 | T → M1 PublicationCorresponds to variant dbSNP:rs776858093Ensembl. | 1 | |
Natural variantiVAR_040856 | 701 | A → T1 PublicationCorresponds to variant dbSNP:rs142835596EnsemblClinVar. | 1 | |
Natural variantiVAR_040857 | 709 | V → M1 PublicationCorresponds to variant dbSNP:rs112537316EnsemblClinVar. | 1 | |
Natural variantiVAR_057108 | 845 | R → C. Corresponds to variant dbSNP:rs3732485EnsemblClinVar. | 1 | |
Natural variantiVAR_019986 | 861 | L → P. Corresponds to variant dbSNP:rs3732486EnsemblClinVar. | 1 | |
Natural variantiVAR_057109 | 877 | V → M. Corresponds to variant dbSNP:rs34542174EnsemblClinVar. | 1 | |
Natural variantiVAR_019987 | 914 | D → E. Corresponds to variant dbSNP:rs3732487EnsemblClinVar. | 1 | |
Natural variantiVAR_065573 | 1085 | T → A1 PublicationCorresponds to variant dbSNP:rs75370906EnsemblClinVar. | 1 | |
Natural variantiVAR_065574 | 1213 | V → M in AAT7; unknown pathological significance. 1 PublicationCorresponds to variant dbSNP:rs368390254EnsemblClinVar. | 1 | |
Natural variantiVAR_065575 | 1399 | E → K1 PublicationCorresponds to variant dbSNP:rs181663420EnsemblClinVar. | 1 | |
Natural variantiVAR_083423 | 1458 – 1914 | Missing in AAT7; unknown pathological significance. 1 PublicationAdd BLAST | 457 | |
Natural variantiVAR_083424 | 1487 – 1914 | Missing in AAT7; unknown pathological significance. 1 PublicationAdd BLAST | 428 | |
Natural variantiVAR_083425 | 1491 | A → S in AAT7; decreases kinase activity. 1 Publication | 1 | |
Natural variantiVAR_040858 | 1527 | A → V1 PublicationCorresponds to variant dbSNP:rs34982967Ensembl. | 1 | |
Natural variantiVAR_040859 | 1588 | P → L in an ovarian mucinous carcinoma sample; somatic mutation. 1 Publication | 1 | |
Natural variantiVAR_065576 | 1754 | A → T in AAT7; 4-fold reduced affinity for calmodulin; decreased kinase activity compared to wild-type protein. 1 Publication | 1 | |
Natural variantiVAR_065577 | 1759 | S → P in AAT7; 7-fold reduced affinity for calmodulin; 6-fold decreased Vmax. 1 PublicationCorresponds to variant dbSNP:rs387906781EnsemblClinVar. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_018846 | 1 – 1760 | Missing in isoform 6 and isoform 8. 3 PublicationsAdd BLAST | 1760 | |
Alternative sequenceiVSP_053791 | 1 – 1200 | Missing in isoform 7. 1 PublicationAdd BLAST | 1200 | |
Alternative sequenceiVSP_018845 | 1 – 922 | Missing in isoform 5 and isoform 9. 2 PublicationsAdd BLAST | 922 | |
Alternative sequenceiVSP_004791 | 437 – 506 | VSGIP…LQVER → G in isoform 2 and isoform 3B. 2 PublicationsAdd BLAST | 70 | |
Alternative sequenceiVSP_004793 | 1473 – 1545 | Missing in isoform 4. 1 PublicationAdd BLAST | 73 | |
Alternative sequenceiVSP_004794 | 1655 – 1705 | Missing in isoform 3A and isoform 3B. 1 PublicationAdd BLAST | 51 | |
Alternative sequenceiVSP_004795 | 1790 | Missing in isoform Del-1790, isoform 8 and isoform 9. 4 Publications | 1 |
Sequence databases
Genome annotation databases
Ensembli | ENST00000346322; ENSP00000320622; ENSG00000065534 [Q15746-2] ENST00000360304; ENSP00000353452; ENSG00000065534 [Q15746-1] ENST00000360772; ENSP00000354004; ENSG00000065534 [Q15746-3] ENST00000418370; ENSP00000428967; ENSG00000065534 [Q15746-8] ENST00000578202; ENSP00000463691; ENSG00000065534 [Q15746-10] ENST00000583087; ENSP00000462118; ENSG00000065534 [Q15746-8] |
GeneIDi | 4638 |
KEGGi | hsa:4638 |
UCSCi | uc003egl.4, human [Q15746-1] |
Keywords - Coding sequence diversityi
Alternative promoter usage, Alternative splicingSimilar proteinsi
Cross-referencesi
Web resourcesi
Wikipedia Myosin light-chain kinase entry |
Atlas of Genetics and Cytogenetics in Oncology and Haematology |
Sequence databases
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2CQV | NMR | - | A | 1238-1338 | [»] | |
2K0F | NMR | - | B | 1742-1760 | [»] | |
2YR3 | NMR | - | A | 510-601 | [»] | |
5JQA | X-ray | 1.80 | B | 1742-1761 | [»] | |
5JTH | X-ray | 1.84 | B | 1742-1761 | [»] | |
6C6M | X-ray | 2.50 | A/B/C | 405-507 | [»] | |
SMRi | Q15746 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 110722, 23 interactors |
CORUMi | Q15746 |
IntActi | Q15746, 28 interactors |
MINTi | Q15746 |
STRINGi | 9606.ENSP00000353452 |
Chemistry databases
BindingDBi | Q15746 |
ChEMBLi | CHEMBL2428 |
DrugBanki | DB12010, Fostamatinib |
DrugCentrali | Q15746 |
GuidetoPHARMACOLOGYi | 1552 |
PTM databases
iPTMneti | Q15746 |
MetOSitei | Q15746 |
PhosphoSitePlusi | Q15746 |
Genetic variation databases
BioMutai | MYLK |
DMDMi | 300669714 |
Proteomic databases
EPDi | Q15746 |
jPOSTi | Q15746 |
MassIVEi | Q15746 |
MaxQBi | Q15746 |
PaxDbi | Q15746 |
PeptideAtlasi | Q15746 |
PRIDEi | Q15746 |
ProteomicsDBi | 5180 60733 [Q15746-1] 60734 [Q15746-2] 60735 [Q15746-3] 60736 [Q15746-4] 60737 [Q15746-5] 60738 [Q15746-6] 60739 [Q15746-7] 60740 [Q15746-8] |
Protocols and materials databases
Antibodypediai | 4044, 355 antibodies |
DNASUi | 4638 |
Genome annotation databases
Ensembli | ENST00000346322; ENSP00000320622; ENSG00000065534 [Q15746-2] ENST00000360304; ENSP00000353452; ENSG00000065534 [Q15746-1] ENST00000360772; ENSP00000354004; ENSG00000065534 [Q15746-3] ENST00000418370; ENSP00000428967; ENSG00000065534 [Q15746-8] ENST00000578202; ENSP00000463691; ENSG00000065534 [Q15746-10] ENST00000583087; ENSP00000462118; ENSG00000065534 [Q15746-8] |
GeneIDi | 4638 |
KEGGi | hsa:4638 |
UCSCi | uc003egl.4, human [Q15746-1] |
Organism-specific databases
CTDi | 4638 |
DisGeNETi | 4638 |
GeneCardsi | MYLK |
GeneReviewsi | MYLK |
HGNCi | HGNC:7590, MYLK |
HPAi | ENSG00000065534, Tissue enhanced (smooth) |
MalaCardsi | MYLK |
MIMi | 249210, phenotype 600922, gene 613780, phenotype |
neXtProti | NX_Q15746 |
OpenTargetsi | ENSG00000065534 |
Orphaneti | 91387, Familial thoracic aortic aneurysm and aortic dissection 2241, Megacystis-microcolon-intestinal hypoperistalsis syndrome |
PharmGKBi | PA31388 |
VEuPathDBi | HostDB:ENSG00000065534.18 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG0613, Eukaryota |
GeneTreei | ENSGT00940000157879 |
HOGENOMi | CLU_000288_76_2_1 |
InParanoidi | Q15746 |
OMAi | DVNCKKG |
OrthoDBi | 330091at2759 |
PhylomeDBi | Q15746 |
TreeFami | TF314166 |
Enzyme and pathway databases
BRENDAi | 2.7.11.18, 2681 |
PathwayCommonsi | Q15746 |
Reactomei | R-HSA-445355, Smooth Muscle Contraction R-HSA-5627123, RHO GTPases activate PAKs |
SABIO-RKi | Q15746 |
SignaLinki | Q15746 |
SIGNORi | Q15746 |
Miscellaneous databases
BioGRID-ORCSi | 4638, 2 hits in 878 CRISPR screens |
ChiTaRSi | MYLK, human |
EvolutionaryTracei | Q15746 |
GeneWikii | MYLK |
GenomeRNAii | 4638 |
Pharosi | Q15746, Tchem |
PROi | PR:Q15746 |
RNActi | Q15746, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000065534, Expressed in cauda epididymis and 252 other tissues |
ExpressionAtlasi | Q15746, baseline and differential |
Genevisiblei | Q15746, HS |
Family and domain databases
CDDi | cd00063, FN3, 1 hit cd14191, STKc_MLCK1, 1 hit |
Gene3Di | 2.60.40.10, 10 hits |
InterProi | View protein in InterPro IPR003961, FN3_dom IPR036116, FN3_sf IPR007110, Ig-like_dom IPR036179, Ig-like_dom_sf IPR013783, Ig-like_fold IPR013098, Ig_I-set IPR003599, Ig_sub IPR003598, Ig_sub2 IPR011009, Kinase-like_dom_sf IPR015725, MLCK1_kinase_dom IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR008271, Ser/Thr_kinase_AS |
Pfami | View protein in Pfam PF00041, fn3, 1 hit PF07679, I-set, 9 hits PF00069, Pkinase, 1 hit |
SMARTi | View protein in SMART SM00060, FN3, 1 hit SM00409, IG, 9 hits SM00408, IGc2, 9 hits SM00220, S_TKc, 1 hit |
SUPFAMi | SSF48726, SSF48726, 9 hits SSF49265, SSF49265, 1 hit SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS50853, FN3, 1 hit PS50835, IG_LIKE, 9 hits PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | MYLK_HUMAN | |
Accessioni | Q15746Primary (citable) accession number: Q15746 Secondary accession number(s): B4DUE3 Q9UIT9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 15, 1998 |
Last sequence update: | July 13, 2010 | |
Last modified: | February 10, 2021 | |
This is version 219 of the entry and version 4 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- UniProtKB entry view manual
User manual for the UniProtKB entry view - Human and mouse protein kinases
Human and mouse protein kinases: classification and index - Human chromosome 3
Human chromosome 3: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families