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Protein

NGFI-A-binding protein 2

Gene

NAB2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Acts as a transcriptional repressor for zinc finger transcription factors EGR1 and EGR2. Isoform 2 lacks repression ability (By similarity).By similarity

GO - Molecular functioni

  • identical protein binding Source: IntAct
  • transcription corepressor activity Source: ProtInc
  • transcription factor binding Source: Ensembl

GO - Biological processi

Keywordsi

Molecular functionRepressor
Biological processTranscription, Transcription regulation

Enzyme and pathway databases

SIGNORiQ15742

Names & Taxonomyi

Protein namesi
Recommended name:
NGFI-A-binding protein 2
Alternative name(s):
EGR-1-binding protein 2
Melanoma-associated delayed early response protein
Short name:
Protein MADER
Gene namesi
Name:NAB2
Synonyms:MADER
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 12

Organism-specific databases

EuPathDBiHostDB:ENSG00000166886.12
HGNCiHGNC:7627 NAB2
MIMi602381 gene
neXtProtiNX_Q15742

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi4665
MalaCardsiNAB2
OpenTargetsiENSG00000166886
Orphaneti2126 Solitary fibrous tumor
PharmGKBiPA31432

Polymorphism and mutation databases

BioMutaiNAB2
DMDMi12643729

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000770421 – 525NGFI-A-binding protein 2Add BLAST525

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei6PhosphoserineCombined sources1
Modified residuei157PhosphoserineBy similarity1
Modified residuei159PhosphoserineCombined sources1
Modified residuei162PhosphoserineCombined sources1
Modified residuei171PhosphoserineCombined sources1
Cross-linki379Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1)1 Publication
Modified residuei479PhosphoserineBy similarity1
Cross-linki517Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1); alternate1 Publication
Cross-linki517Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateCombined sources

Post-translational modificationi

Sumoylation by EGR2 represses EGR2 transcriptional activity in hindbrain.1 Publication

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ15742
MaxQBiQ15742
PaxDbiQ15742
PeptideAtlasiQ15742
PRIDEiQ15742
ProteomicsDBi60728
60729 [Q15742-2]
60730 [Q15742-3]

PTM databases

iPTMnetiQ15742
PhosphoSitePlusiQ15742

Expressioni

Tissue specificityi

Widely expressed at low levels. Highly expressed in melanoma cell lines.

Inductioni

By serum and phorbol myristate acetate (PMA) stimulation.

Gene expression databases

BgeeiENSG00000166886
CleanExiHS_NAB2
GenevisibleiQ15742 HS

Organism-specific databases

HPAiCAB004510
HPA027464

Interactioni

Subunit structurei

Homomultimers may associate with EGR1 bound to DNA.By similarity

Binary interactionsi

Show more details

GO - Molecular functioni

  • identical protein binding Source: IntAct
  • transcription factor binding Source: Ensembl

Protein-protein interaction databases

BioGridi110747, 46 interactors
IntActiQ15742, 70 interactors
MINTiQ15742
STRINGi9606.ENSP00000300131

Structurei

3D structure databases

ProteinModelPortaliQ15742
SMRiQ15742
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni35 – 113NCD1Add BLAST79
Regioni267 – 356NCD2Add BLAST90
Regioni353 – 384Necessary for nuclear localizationBy similarityAdd BLAST32

Domaini

The NAB conserved domain 1 (NCD1) interacts with EGR1 inhibitory domain and mediates multimerization.
The NAB conserved domain 2 (NCD2) is necessary for transcriptional repression.

Sequence similaritiesi

Belongs to the NAB family.Curated

Phylogenomic databases

eggNOGiKOG3835 Eukaryota
ENOG410YKFH LUCA
GeneTreeiENSGT00390000006330
HOGENOMiHOG000026777
HOVERGENiHBG003127
InParanoidiQ15742
KOiK22388
OMAiHARTNAD
OrthoDBiEOG091G0NQE
PhylomeDBiQ15742
TreeFamiTF315501

Family and domain databases

Gene3Di3.40.390.60, 1 hit
InterProiView protein in InterPro
IPR006989 NAB_co-repressor_dom
IPR039040 NAB_fam
IPR006988 Nab_N
IPR038398 NCD2_sf
PANTHERiPTHR12623 PTHR12623, 1 hit
PfamiView protein in Pfam
PF04904 NCD1, 1 hit
PF04905 NCD2, 1 hit
ProDomiView protein in ProDom or Entries sharing at least one domain
PD342192 Nab_N, 1 hit

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q15742-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MHRAPSPTAE QPPGGGDSAR RTLQPRLKPS ARAMALPRTL GELQLYRVLQ
60 70 80 90 100
RANLLSYYET FIQQGGDDVQ QLCEAGEEEF LEIMALVGMA TKPLHVRRLQ
110 120 130 140 150
KALREWATNP GLFSQPVPAV PVSSIPLFKI SETAGTRKGS MSNGHGSPGE
160 170 180 190 200
KAGSARSFSP KSPLELGEKL SPLPGGPGAG DPRIWPGRST PESDVGAGGE
210 220 230 240 250
EEAGSPPFSP PAGGGVPEGT GAGGLAAGGT GGGPDRLEPE MVRMVVESVE
260 270 280 290 300
RIFRSFPRGD AGEVTSLLKL NKKLARSVGH IFEMDDNDSQ KEEEIRKYSI
310 320 330 340 350
IYGRFDSKRR EGKQLSLHEL TINEAAAQFC MRDNTLLLRR VELFSLSRQV
360 370 380 390 400
ARESTYLSSL KGSRLHPEEL GGPPLKKLKQ EVGEQSHPEI QQPPPGPESY
410 420 430 440 450
VPPYRPSLEE DSASLSGESL DGHLQAVGSC PRLTPPPADL PLALPAHGLW
460 470 480 490 500
SRHILQQTLM DEGLRLARLV SHDRVGRLSP CVPAKPPLAE FEEGLLDRCP
510 520
APGPHPALVE GRRSSVKVEA EASRQ
Length:525
Mass (Da):56,594
Last modified:November 1, 1996 - v1
Checksum:i38CF6CFEFE3756F9
GO
Isoform 2 (identifier: Q15742-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     320-322: LTI → ASP
     323-525: Missing.

Show »
Length:322
Mass (Da):34,356
Checksum:i6C7ACFBFEAD113E0
GO
Isoform 3 (identifier: Q15742-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     426-489: Missing.

Show »
Length:461
Mass (Da):49,748
Checksum:iF7C955EEFDAFBA94
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti257 – 258PR → Q (PubMed:8649813).Curated2
Sequence conflicti257 – 258PR → Q (Ref. 7) Curated2

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_003385320 – 322LTI → ASP in isoform 2. 1 Publication3
Alternative sequenceiVSP_003386323 – 525Missing in isoform 2. 1 PublicationAdd BLAST203
Alternative sequenceiVSP_003387426 – 489Missing in isoform 3. CuratedAdd BLAST64

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U48361 mRNA Translation: AAC50589.1
AF268380 Genomic DNA Translation: AAF72545.1
AK314229 mRNA Translation: BAG36900.1
CH471054 Genomic DNA Translation: EAW96989.1
BC065931 mRNA Translation: AAH65931.1
X70991 mRNA Translation: CAA50318.1
AJ011081 Genomic DNA Translation: CAA09472.1
CCDSiCCDS81701.1 [Q15742-3]
CCDS8930.1 [Q15742-1]
RefSeqiNP_001317234.1, NM_001330305.1 [Q15742-3]
NP_005958.1, NM_005967.3 [Q15742-1]
UniGeneiHs.159223

Genome annotation databases

EnsembliENST00000300131; ENSP00000300131; ENSG00000166886 [Q15742-1]
ENST00000342556; ENSP00000341491; ENSG00000166886 [Q15742-3]
GeneIDi4665
KEGGihsa:4665
UCSCiuc001smz.3 human [Q15742-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiNAB2_HUMAN
AccessioniPrimary (citable) accession number: Q15742
Secondary accession number(s): B2RAK3, O76006, Q14797
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: November 1, 1996
Last modified: July 18, 2018
This is version 152 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 12
    Human chromosome 12: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

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