ZRSR2

Functionⁱ

Pre-mRNA-binding protein required for splicing of both U2- and U12-type introns. Selectively interacts with the 3'-splice site of U2- and U12-type pre-mRNAs and promotes different steps in U2 and U12 intron splicing. Recruited to U12 pre-mRNAs in an ATP-dependent manner and is required for assembly of the prespliceosome, a precursor to other spliceosomal complexes. For U2-type introns, it is selectively and specifically required for the second step of splicing.2 Publications

Regions

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Zinc fingerⁱ	166 – 194	C3H1-type 1PROSITE-ProRule annotationAdd BLAST		29
Zinc fingerⁱ	306 – 333	C3H1-type 2PROSITE-ProRule annotationAdd BLAST		28

GO - Molecular functionⁱ

metal ion binding Source: UniProtKB-KW
pre-mRNA 3'-splice site binding
Source: UniProtKB
Inferred from direct assayⁱ
- 21041408

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

mRNA splicing, via spliceosome
Source: UniProtKB
Inferred from mutant phenotypeⁱ
- 9237760
RNA splicing
Source: HGNC
Inferred by curatorⁱ
- 15146077
spliceosomal complex assembly
Source: UniProtKB
Inferred from mutant phenotypeⁱ
- 21041408

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Ribonucleoprotein, RNA-binding
Biological process	mRNA processing, mRNA splicing
Ligand	Metal-binding, Zinc

Enzyme and pathway databases

Reactomeⁱ	R-HSA-72165 mRNA Splicing - Minor Pathway
SIGNORⁱ	Q15696

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: U2 small nuclear ribonucleoprotein auxiliary factor 35 kDa subunit-related protein 2 Alternative name(s): CCCH type zinc finger, RNA-binding motif and serine/arginine rich protein 2 Renal carcinoma antigen NY-REN-20 U2(RNU2) small nuclear RNA auxiliary factor 1-like 2 U2AF35-related protein Short name: URP
Gene namesⁱ	Name:ZRSR2 Synonyms:U2AF1-RS2, U2AF1L2, U2AF1RS2, URP
Organismⁱ	Homo sapiens (Human)
Taxonomic identifierⁱ	9606 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo
Proteomesⁱ	UP000005640 Componentⁱ: Chromosome X

Organism-specific databases

EuPathDBⁱ	HostDB:ENSG00000169249.12
Human Gene Nomenclature Database More...HGNCⁱ	HGNC:23019 ZRSR2
MIMⁱ	300028 gene
neXtProtⁱ	NX_Q15696

Keywords - Cellular componentⁱ

Nucleus, Spliceosome

Pathology & Biotechⁱ

Organism-specific databases

DisGeNET More...DisGeNETⁱ	8233
Open Targets More...OpenTargetsⁱ	ENSG00000169249
PharmGKBⁱ	PA162410930

Polymorphism and mutation databases

BioMutaⁱ	ZRSR2
DMDMⁱ	2833266

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000082001	1 – 482	U2 small nuclear ribonucleoprotein auxiliary factor 35 kDa subunit-related protein 2Add BLAST		482

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Length
Cross-linkⁱ	45	Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linkⁱ	62	Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residueⁱ	349	PhosphoserineCombined sources	1
Modified residueⁱ	384	PhosphoserineCombined sources	1

Post-translational modificationⁱ

Phosphorylated in the RS domain by SRPK1.1 Publication

Keywords - PTMⁱ

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDⁱ	Q15696
MaxQB - The MaxQuant DataBase More...MaxQBⁱ	Q15696
PaxDbⁱ	Q15696
PeptideAtlas More...PeptideAtlasⁱ	Q15696
PRIDEⁱ	Q15696
ProteomicsDBⁱ	60704

PTM databases

iPTMnetⁱ	Q15696
PhosphoSitePlusⁱ	Q15696

Expressionⁱ

Tissue specificityⁱ

Widely expressed.1 Publication

Gene expression databases

Bgeeⁱ	ENSG00000169249 Expressed in 226 organ(s), highest expression level in frontal cortex
CleanExⁱ	HS_ZRSR2
ExpressionAtlasⁱ	Q15696 baseline and differential
Genevisibleⁱ	Q15696 HS

Interactionⁱ

Subunit structureⁱ

Component of the U11/U12 snRNPs that are part of the U12-type spliceosome. Interacts (via RS domain) with SRSF1 and SRSF2. Interacts with U2AF2/U2AF65.3 Publications

Binary interactionsⁱ

With	Entry	#Exp.	IntAct
CLK2	P49760	5	EBI-6657923,EBI-750020
SDCBP2	Q9H190	5	EBI-6657923,EBI-742426
SRPK2	P78362	6	EBI-6657923,EBI-593303
SSX2IP	Q9Y2D8	3	EBI-6657923,EBI-2212028

Protein-protein interaction databases

BioGridⁱ	113865, 47 interactors
CORUMⁱ	Q15696
Database of interacting proteins More...DIPⁱ	DIP-62117N
IntActⁱ	Q15696, 17 interactors
STRINGⁱ	9606.ENSP00000303015

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	Q15696
SMRⁱ	Q15696
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Domainⁱ	198 – 304	RRMPROSITE-ProRule annotationAdd BLAST		107

Compositional bias

Feature key	Position(s)	DescriptionActions	Length
Compositional biasⁱ	46 – 49	Poly-Glu	4
Compositional biasⁱ	118 – 123	Poly-Glu	6
Compositional biasⁱ	414 – 469	Arg/Ser-rich (RS domain)Add BLAST	56

Zinc finger

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Zinc fingerⁱ	166 – 194	C3H1-type 1PROSITE-ProRule annotationAdd BLAST		29
Zinc fingerⁱ	306 – 333	C3H1-type 2PROSITE-ProRule annotationAdd BLAST		28

Keywords - Domainⁱ

Repeat, Zinc-finger

Phylogenomic databases

eggNOGⁱ	KOG2202 Eukaryota ENOG410Z5PX LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00530000063193
HOGENOMⁱ	HOG000049279
HOVERGENⁱ	HBG054605
InParanoidⁱ	Q15696
OMAⁱ	SPSHSYK
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G0CUI
PhylomeDBⁱ	Q15696
TreeFamⁱ	TF324447

Family and domain databases

Gene3Dⁱ	3.30.70.330, 1 hit
InterProⁱ	View protein in InterPro IPR012677 Nucleotide-bd_a/b_plait_sf IPR035979 RBD_domain_sf IPR000504 RRM_dom IPR003954 RRM_dom_euk IPR009145 U2AF_small IPR000571 Znf_CCCH
PANTHERⁱ	PTHR12620 PTHR12620, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00076 RRM_1, 1 hit PF00642 zf-CCCH, 2 hits
PRINTSⁱ	PR01848 U2AUXFACTOR
SMARTⁱ	View protein in SMART SM00361 RRM_1, 1 hit SM00356 ZnF_C3H1, 2 hits
SUPFAMⁱ	SSF54928 SSF54928, 1 hit
PROSITEⁱ	View protein in PROSITE PS50102 RRM, 1 hit PS50103 ZF_C3H1, 2 hits

Sequence (1+)ⁱ

Sequence statusⁱ: Complete.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show all Align All

Q15696-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MAAPEKMTFP EKPSHKKYRA ALKKEKRKKR RQELARLRDS GLSQKEEEED 
        60         70         80         90        100
TFIEEQQLEE EKLLERERQR LHEEWLLREQ KAQEEFRIKK EKEEAAKKRQ 
       110        120        130        140        150
EEQERKLKEQ WEEQQRKERE EEEQKRQEKK EKEEALQKML DQAENELENG 
       160        170        180        190        200
TTWQNPEPPV DFRVMEKDRA NCPFYSKTGA CRFGDRCSRK HNFPTSSPTL 
       210        220        230        240        250
LIKSMFTTFG MEQCRRDDYD PDASLEYSEE ETYQQFLDFY EDVLPEFKNV 
       260        270        280        290        300
GKVIQFKVSC NLEPHLRGNV YVQYQSEEEC QAALSLFNGR WYAGRQLQCE 
       310        320        330        340        350
FCPVTRWKMA ICGLFEIQQC PRGKHCNFLH VFRNPNNEFW EANRDIYLSP 
       360        370        380        390        400
DRTGSSFGKN SERRERMGHH DDYYSRLRGR RNPSPDHSYK RNGESERKSS 
       410        420        430        440        450
RHRGKKSHKR TSKSRERHNS RSRGRNRDRS RDRSRGRGSR SRSRSRSRRS 
       460        470        480 
RRSRSQSSSR SRSRGRRRSG NRDRTVQSPK SK

Length:482

Mass (Da):58,045

Last modified:May 1, 1997 - v2

Checksum:ⁱ1DACC8A6CA4727A6

GO

Computationally mapped potential isoform sequencesⁱ

There is 1 potential isoform mapped to this entry.BLAST Align Show all Add to basket

	Entry	Entry name		Protein names	Gene names	Length	Annotation

	A6NDW0	A6NDW0_HUMAN		U2 small nuclear ribonucleoprotein ... U2 small nuclear ribonucleoprotein auxiliary factor 35 kDa subunit-related protein 2	ZRSR2	147	Annotation score:

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	D49677 mRNA Translation: BAA08533.1 AC004106 Genomic DNA No translation available. AC096510 Genomic DNA No translation available. BC113454 mRNA Translation: AAI13455.1 BC113480 mRNA Translation: AAI13481.1
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS14172.1
NCBI Reference Sequences More...RefSeqⁱ	NP_005080.1, NM_005089.3
UniGeneⁱ	Hs.171909

Genome annotation databases

Ensemblⁱ	ENST00000307771; ENSP00000303015; ENSG00000169249
GeneIDⁱ	8233
KEGGⁱ	hsa:8233
UCSC genome browser More...UCSCⁱ	uc004cxg.5 human

Similar proteinsⁱ

90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
Q15696	Putative U2 small nuclear ribonucleoprotein auxiliary factor 35 kDa subunit-related protein 1		HUMAN		479	UniRef90_Q15696
Uncharacterized protein		PAPAN		485
Uncharacterized protein		CERAT		484
Uncharacterized protein		CHLSB		482
Uncharacterized protein		NOMLE		482
+47

Protein	Similar proteins		Species	Score	Length	Source
Q15696	Putative U2 small nuclear ribonucleoprotein auxiliary factor 35 kDa subunit-related protein 1		HUMAN		479	UniRef50_Q15696
Uncharacterized protein		PAPAN		485
Uncharacterized protein		CERAT		484
Uncharacterized protein		CHLSB		482
Uncharacterized protein		NOMLE		482
+126

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	D49677 mRNA Translation: BAA08533.1 AC004106 Genomic DNA No translation available. AC096510 Genomic DNA No translation available. BC113454 mRNA Translation: AAI13455.1 BC113480 mRNA Translation: AAI13481.1
CCDSⁱ	CCDS14172.1
RefSeqⁱ	NP_005080.1, NM_005089.3
UniGeneⁱ	Hs.171909

3D structure databases

ProteinModelPortalⁱ	Q15696
SMRⁱ	Q15696
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	113865, 47 interactors
CORUMⁱ	Q15696
DIPⁱ	DIP-62117N
IntActⁱ	Q15696, 17 interactors
STRINGⁱ	9606.ENSP00000303015

PTM databases

iPTMnetⁱ	Q15696
PhosphoSitePlusⁱ	Q15696

Polymorphism and mutation databases

BioMutaⁱ	ZRSR2
DMDMⁱ	2833266

Proteomic databases

EPDⁱ	Q15696
MaxQBⁱ	Q15696
PaxDbⁱ	Q15696
PeptideAtlasⁱ	Q15696
PRIDEⁱ	Q15696
ProteomicsDBⁱ	60704

Protocols and materials databases

The DNASU plasmid repository More...DNASUⁱ	8233
Structural Biology Knowledgebase	Search...

Genome annotation databases

Ensemblⁱ	ENST00000307771; ENSP00000303015; ENSG00000169249
GeneIDⁱ	8233
KEGGⁱ	hsa:8233
UCSCⁱ	uc004cxg.5 human

Organism-specific databases

CTDⁱ	8233
DisGeNETⁱ	8233
EuPathDBⁱ	HostDB:ENSG00000169249.12
GeneCardsⁱ	ZRSR2
H-InvDBⁱ	HIX0017758
HGNCⁱ	HGNC:23019 ZRSR2
MIMⁱ	300028 gene
neXtProtⁱ	NX_Q15696
OpenTargetsⁱ	ENSG00000169249
PharmGKBⁱ	PA162410930
GenAtlas: human gene database More...GenAtlasⁱ	Search...

Phylogenomic databases

eggNOGⁱ	KOG2202 Eukaryota ENOG410Z5PX LUCA
GeneTreeⁱ	ENSGT00530000063193
HOGENOMⁱ	HOG000049279
HOVERGENⁱ	HBG054605
InParanoidⁱ	Q15696
OMAⁱ	SPSHSYK
OrthoDBⁱ	EOG091G0CUI
PhylomeDBⁱ	Q15696
TreeFamⁱ	TF324447

Enzyme and pathway databases

Reactomeⁱ	R-HSA-72165 mRNA Splicing - Minor Pathway
SIGNORⁱ	Q15696

Miscellaneous databases

ChiTaRSⁱ	ZRSR2 human
GeneWikiⁱ	ZRSR2
GenomeRNAiⁱ	8233
Protein Ontology More...PROⁱ	PR:Q15696
SOURCEⁱ	Search...

Gene expression databases

Bgeeⁱ	ENSG00000169249 Expressed in 226 organ(s), highest expression level in frontal cortex
CleanExⁱ	HS_ZRSR2
ExpressionAtlasⁱ	Q15696 baseline and differential
Genevisibleⁱ	Q15696 HS

Family and domain databases

Gene3Dⁱ	3.30.70.330, 1 hit
InterProⁱ	View protein in InterPro IPR012677 Nucleotide-bd_a/b_plait_sf IPR035979 RBD_domain_sf IPR000504 RRM_dom IPR003954 RRM_dom_euk IPR009145 U2AF_small IPR000571 Znf_CCCH
PANTHERⁱ	PTHR12620 PTHR12620, 1 hit
Pfamⁱ	View protein in Pfam PF00076 RRM_1, 1 hit PF00642 zf-CCCH, 2 hits
PRINTSⁱ	PR01848 U2AUXFACTOR
SMARTⁱ	View protein in SMART SM00361 RRM_1, 1 hit SM00356 ZnF_C3H1, 2 hits
SUPFAMⁱ	SSF54928 SSF54928, 1 hit
PROSITEⁱ	View protein in PROSITE PS50102 RRM, 1 hit PS50103 ZF_C3H1, 2 hits
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	U2AFM_HUMAN
Accessionⁱ	Q15696Primary (citable) accession number: Q15696 Secondary accession number(s): Q14D69
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
	Last sequence update:	May 1, 1997
	Last modified:	November 7, 2018
	This is version 164 of the entry and version 2 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program
Disclaimer	Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

Documents

MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
Human chromosome X
Human chromosome X: entries, gene names and cross-references to MIM

UniProtKB

UniParc

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Supporting data

UniProtKB - Q15696 (U2AFM_HUMAN)

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U2 small nuclear ribonucleoprotein auxiliary factor 35 kDa subunit-related protein 2

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Regions

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Nucleus

Nucleus

Keywords - Cellular componenti

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Organism-specific databases

Polymorphism and mutation databases

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Keywords - PTMi

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Compositional bias

Zinc finger

Keywords - Domaini

Phylogenomic databases

Family and domain databases

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Polymorphism and mutation databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Documents

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Expressionⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Keywords - Domainⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ