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Protein

U2 small nuclear ribonucleoprotein auxiliary factor 35 kDa subunit-related protein 2

Gene

ZRSR2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Pre-mRNA-binding protein required for splicing of both U2- and U12-type introns. Selectively interacts with the 3'-splice site of U2- and U12-type pre-mRNAs and promotes different steps in U2 and U12 intron splicing. Recruited to U12 pre-mRNAs in an ATP-dependent manner and is required for assembly of the prespliceosome, a precursor to other spliceosomal complexes. For U2-type introns, it is selectively and specifically required for the second step of splicing.2 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri166 – 194C3H1-type 1PROSITE-ProRule annotationAdd BLAST29
Zinc fingeri306 – 333C3H1-type 2PROSITE-ProRule annotationAdd BLAST28

GO - Molecular functioni

  • metal ion binding Source: UniProtKB-KW
  • pre-mRNA 3'-splice site binding Source: UniProtKB

GO - Biological processi

  • mRNA splicing, via spliceosome Source: UniProtKB
  • RNA splicing Source: HGNC
  • spliceosomal complex assembly Source: UniProtKB

Keywordsi

Molecular functionRibonucleoprotein, RNA-binding
Biological processmRNA processing, mRNA splicing
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-HSA-72165 mRNA Splicing - Minor Pathway
SIGNORiQ15696

Names & Taxonomyi

Protein namesi
Recommended name:
U2 small nuclear ribonucleoprotein auxiliary factor 35 kDa subunit-related protein 2
Alternative name(s):
CCCH type zinc finger, RNA-binding motif and serine/arginine rich protein 2
Renal carcinoma antigen NY-REN-20
U2(RNU2) small nuclear RNA auxiliary factor 1-like 2
U2AF35-related protein
Short name:
URP
Gene namesi
Name:ZRSR2
Synonyms:U2AF1-RS2, U2AF1L2, U2AF1RS2, URP
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome X

Organism-specific databases

EuPathDBiHostDB:ENSG00000169249.12
HGNCiHGNC:23019 ZRSR2
MIMi300028 gene
neXtProtiNX_Q15696

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus, Spliceosome

Pathology & Biotechi

Organism-specific databases

DisGeNETi8233
OpenTargetsiENSG00000169249
PharmGKBiPA162410930

Polymorphism and mutation databases

BioMutaiZRSR2
DMDMi2833266

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000820011 – 482U2 small nuclear ribonucleoprotein auxiliary factor 35 kDa subunit-related protein 2Add BLAST482

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki45Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki62Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei349PhosphoserineCombined sources1
Modified residuei384PhosphoserineCombined sources1

Post-translational modificationi

Phosphorylated in the RS domain by SRPK1.1 Publication

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ15696
MaxQBiQ15696
PaxDbiQ15696
PeptideAtlasiQ15696
PRIDEiQ15696
ProteomicsDBi60704

PTM databases

iPTMnetiQ15696
PhosphoSitePlusiQ15696

Expressioni

Tissue specificityi

Widely expressed.1 Publication

Gene expression databases

BgeeiENSG00000169249
CleanExiHS_ZRSR2
ExpressionAtlasiQ15696 baseline and differential
GenevisibleiQ15696 HS

Interactioni

Subunit structurei

Component of the U11/U12 snRNPs that are part of the U12-type spliceosome. Interacts (via RS domain) with SRSF1 and SRSF2. Interacts with U2AF2/U2AF65.3 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi113865, 47 interactors
CORUMiQ15696
DIPiDIP-62117N
IntActiQ15696, 17 interactors
STRINGi9606.ENSP00000303015

Structurei

3D structure databases

ProteinModelPortaliQ15696
SMRiQ15696
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini198 – 304RRMPROSITE-ProRule annotationAdd BLAST107

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi46 – 49Poly-Glu4
Compositional biasi118 – 123Poly-Glu6
Compositional biasi414 – 469Arg/Ser-rich (RS domain)Add BLAST56

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri166 – 194C3H1-type 1PROSITE-ProRule annotationAdd BLAST29
Zinc fingeri306 – 333C3H1-type 2PROSITE-ProRule annotationAdd BLAST28

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG2202 Eukaryota
ENOG410Z5PX LUCA
GeneTreeiENSGT00530000063193
HOGENOMiHOG000049279
HOVERGENiHBG054605
InParanoidiQ15696
OMAiKTAICGL
OrthoDBiEOG091G0CUI
PhylomeDBiQ15696
TreeFamiTF324447

Family and domain databases

Gene3Di3.30.70.330, 1 hit
InterProiView protein in InterPro
IPR012677 Nucleotide-bd_a/b_plait_sf
IPR035979 RBD_domain_sf
IPR000504 RRM_dom
IPR003954 RRM_dom_euk
IPR009145 U2AF_small
IPR000571 Znf_CCCH
PANTHERiPTHR12620 PTHR12620, 1 hit
PfamiView protein in Pfam
PF00076 RRM_1, 1 hit
PF00642 zf-CCCH, 2 hits
PRINTSiPR01848 U2AUXFACTOR
SMARTiView protein in SMART
SM00361 RRM_1, 1 hit
SM00356 ZnF_C3H1, 2 hits
SUPFAMiSSF54928 SSF54928, 1 hit
PROSITEiView protein in PROSITE
PS50102 RRM, 1 hit
PS50103 ZF_C3H1, 2 hits

Sequencei

Sequence statusi: Complete.

Q15696-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAPEKMTFP EKPSHKKYRA ALKKEKRKKR RQELARLRDS GLSQKEEEED
60 70 80 90 100
TFIEEQQLEE EKLLERERQR LHEEWLLREQ KAQEEFRIKK EKEEAAKKRQ
110 120 130 140 150
EEQERKLKEQ WEEQQRKERE EEEQKRQEKK EKEEALQKML DQAENELENG
160 170 180 190 200
TTWQNPEPPV DFRVMEKDRA NCPFYSKTGA CRFGDRCSRK HNFPTSSPTL
210 220 230 240 250
LIKSMFTTFG MEQCRRDDYD PDASLEYSEE ETYQQFLDFY EDVLPEFKNV
260 270 280 290 300
GKVIQFKVSC NLEPHLRGNV YVQYQSEEEC QAALSLFNGR WYAGRQLQCE
310 320 330 340 350
FCPVTRWKMA ICGLFEIQQC PRGKHCNFLH VFRNPNNEFW EANRDIYLSP
360 370 380 390 400
DRTGSSFGKN SERRERMGHH DDYYSRLRGR RNPSPDHSYK RNGESERKSS
410 420 430 440 450
RHRGKKSHKR TSKSRERHNS RSRGRNRDRS RDRSRGRGSR SRSRSRSRRS
460 470 480
RRSRSQSSSR SRSRGRRRSG NRDRTVQSPK SK
Length:482
Mass (Da):58,045
Last modified:May 1, 1997 - v2
Checksum:i1DACC8A6CA4727A6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D49677 mRNA Translation: BAA08533.1
AC004106 Genomic DNA No translation available.
AC096510 Genomic DNA No translation available.
BC113454 mRNA Translation: AAI13455.1
BC113480 mRNA Translation: AAI13481.1
CCDSiCCDS14172.1
RefSeqiNP_005080.1, NM_005089.3
UniGeneiHs.171909

Genome annotation databases

EnsembliENST00000307771; ENSP00000303015; ENSG00000169249
GeneIDi8233
KEGGihsa:8233
UCSCiuc004cxg.5 human

Similar proteinsi

Entry informationi

Entry nameiU2AFM_HUMAN
AccessioniPrimary (citable) accession number: Q15696
Secondary accession number(s): Q14D69
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: May 1, 1997
Last modified: July 18, 2018
This is version 162 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome X
    Human chromosome X: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

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