UniProtKB - Q15696 (U2AFM_HUMAN)
Protein
U2 small nuclear ribonucleoprotein auxiliary factor 35 kDa subunit-related protein 2
Gene
ZRSR2
Organism
Homo sapiens (Human)
Status
Functioni
Pre-mRNA-binding protein required for splicing of both U2- and U12-type introns. Selectively interacts with the 3'-splice site of U2- and U12-type pre-mRNAs and promotes different steps in U2 and U12 intron splicing. Recruited to U12 pre-mRNAs in an ATP-dependent manner and is required for assembly of the prespliceosome, a precursor to other spliceosomal complexes. For U2-type introns, it is selectively and specifically required for the second step of splicing.2 Publications
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 166 – 194 | C3H1-type 1PROSITE-ProRule annotationAdd BLAST | 29 | |
Zinc fingeri | 306 – 333 | C3H1-type 2PROSITE-ProRule annotationAdd BLAST | 28 |
GO - Molecular functioni
- identical protein binding Source: IntAct
- metal ion binding Source: UniProtKB-KW
- pre-mRNA 3'-splice site binding Source: UniProtKB
GO - Biological processi
- mRNA splicing, via spliceosome Source: UniProtKB
- RNA splicing Source: HGNC-UCL
- spliceosomal complex assembly Source: UniProtKB
Keywordsi
Molecular function | Ribonucleoprotein, RNA-binding |
Biological process | mRNA processing, mRNA splicing |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
PathwayCommonsi | Q15696 |
Reactomei | R-HSA-72165, mRNA Splicing - Minor Pathway |
SIGNORi | Q15696 |
Names & Taxonomyi
Protein namesi | Recommended name: U2 small nuclear ribonucleoprotein auxiliary factor 35 kDa subunit-related protein 2Alternative name(s): CCCH type zinc finger, RNA-binding motif and serine/arginine rich protein 2 Renal carcinoma antigen NY-REN-20 U2(RNU2) small nuclear RNA auxiliary factor 1-like 2 U2AF35-related protein Short name: URP |
Gene namesi | Name:ZRSR2 Synonyms:U2AF1-RS2, U2AF1L2, U2AF1RS2, URP |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:23019, ZRSR2 |
MIMi | 300028, gene |
neXtProti | NX_Q15696 |
VEuPathDBi | HostDB:ENSG00000169249.12 |
Subcellular locationi
Nucleus
- Nucleus 1 Publication
Nucleus
- nucleoplasm Source: Reactome
- spliceosomal complex Source: GO_Central
- U12-type spliceosomal complex Source: UniProtKB
- U2AF complex Source: GO_Central
Keywords - Cellular componenti
Nucleus, SpliceosomePathology & Biotechi
Organism-specific databases
DisGeNETi | 8233 |
OpenTargetsi | ENSG00000169249 |
PharmGKBi | PA162410930 |
Miscellaneous databases
Pharosi | Q15696, Tbio |
Genetic variation databases
BioMutai | ZRSR2 |
DMDMi | 2833266 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000082001 | 1 – 482 | U2 small nuclear ribonucleoprotein auxiliary factor 35 kDa subunit-related protein 2Add BLAST | 482 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Cross-linki | 45 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources | ||
Cross-linki | 62 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources | ||
Modified residuei | 349 | PhosphoserineCombined sources | 1 | |
Modified residuei | 384 | PhosphoserineCombined sources | 1 |
Post-translational modificationi
Phosphorylated in the RS domain by SRPK1.1 Publication
Keywords - PTMi
Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
EPDi | Q15696 |
jPOSTi | Q15696 |
MassIVEi | Q15696 |
MaxQBi | Q15696 |
PaxDbi | Q15696 |
PeptideAtlasi | Q15696 |
PRIDEi | Q15696 |
ProteomicsDBi | 60704 |
PTM databases
iPTMneti | Q15696 |
PhosphoSitePlusi | Q15696 |
Expressioni
Tissue specificityi
Widely expressed.1 Publication
Gene expression databases
Bgeei | ENSG00000169249, Expressed in sural nerve and 237 other tissues |
ExpressionAtlasi | Q15696, baseline and differential |
Genevisiblei | Q15696, HS |
Organism-specific databases
HPAi | ENSG00000169249, Low tissue specificity |
Interactioni
Subunit structurei
Component of the U11/U12 snRNPs that are part of the U12-type spliceosome.
Interacts (via RS domain) with SRSF1 and SRSF2.
Interacts with U2AF2/U2AF65.
3 PublicationsBinary interactionsi
Hide detailsQ15696
GO - Molecular functioni
- identical protein binding Source: IntAct
Protein-protein interaction databases
BioGRIDi | 113865, 57 interactors |
CORUMi | Q15696 |
DIPi | DIP-62117N |
IntActi | Q15696, 51 interactors |
STRINGi | 9606.ENSP00000303015 |
Miscellaneous databases
RNActi | Q15696, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 198 – 304 | RRMPROSITE-ProRule annotationAdd BLAST | 107 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 46 – 49 | Poly-Glu | 4 | |
Compositional biasi | 118 – 123 | Poly-Glu | 6 | |
Compositional biasi | 414 – 469 | Arg/Ser-rich (RS domain)Add BLAST | 56 |
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 166 – 194 | C3H1-type 1PROSITE-ProRule annotationAdd BLAST | 29 | |
Zinc fingeri | 306 – 333 | C3H1-type 2PROSITE-ProRule annotationAdd BLAST | 28 |
Keywords - Domaini
Repeat, Zinc-fingerPhylogenomic databases
eggNOGi | KOG2202, Eukaryota |
GeneTreei | ENSGT00950000183152 |
HOGENOMi | CLU_029117_1_0_1 |
InParanoidi | Q15696 |
OMAi | FDTEEQC |
OrthoDBi | 1340384at2759 |
PhylomeDBi | Q15696 |
TreeFami | TF324447 |
Family and domain databases
Gene3Di | 3.30.70.330, 1 hit |
InterProi | View protein in InterPro IPR012677, Nucleotide-bd_a/b_plait_sf IPR035979, RBD_domain_sf IPR000504, RRM_dom IPR003954, RRM_dom_euk IPR009145, U2AF_small IPR000571, Znf_CCCH |
PANTHERi | PTHR12620, PTHR12620, 1 hit |
Pfami | View protein in Pfam PF00076, RRM_1, 1 hit PF00642, zf-CCCH, 2 hits |
PRINTSi | PR01848, U2AUXFACTOR |
SMARTi | View protein in SMART SM00361, RRM_1, 1 hit SM00356, ZnF_C3H1, 2 hits |
SUPFAMi | SSF54928, SSF54928, 1 hit |
PROSITEi | View protein in PROSITE PS50102, RRM, 1 hit PS50103, ZF_C3H1, 2 hits |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
Q15696-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAAPEKMTFP EKPSHKKYRA ALKKEKRKKR RQELARLRDS GLSQKEEEED
60 70 80 90 100
TFIEEQQLEE EKLLERERQR LHEEWLLREQ KAQEEFRIKK EKEEAAKKRQ
110 120 130 140 150
EEQERKLKEQ WEEQQRKERE EEEQKRQEKK EKEEALQKML DQAENELENG
160 170 180 190 200
TTWQNPEPPV DFRVMEKDRA NCPFYSKTGA CRFGDRCSRK HNFPTSSPTL
210 220 230 240 250
LIKSMFTTFG MEQCRRDDYD PDASLEYSEE ETYQQFLDFY EDVLPEFKNV
260 270 280 290 300
GKVIQFKVSC NLEPHLRGNV YVQYQSEEEC QAALSLFNGR WYAGRQLQCE
310 320 330 340 350
FCPVTRWKMA ICGLFEIQQC PRGKHCNFLH VFRNPNNEFW EANRDIYLSP
360 370 380 390 400
DRTGSSFGKN SERRERMGHH DDYYSRLRGR RNPSPDHSYK RNGESERKSS
410 420 430 440 450
RHRGKKSHKR TSKSRERHNS RSRGRNRDRS RDRSRGRGSR SRSRSRSRRS
460 470 480
RRSRSQSSSR SRSRGRRRSG NRDRTVQSPK SK
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA6NDW0 | A6NDW0_HUMAN | U2 small nuclear ribonucleoprotein ... | ZRSR2 | 147 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D49677 mRNA Translation: BAA08533.1 AC004106 Genomic DNA No translation available. AC096510 Genomic DNA No translation available. BC113454 mRNA Translation: AAI13455.1 BC113480 mRNA Translation: AAI13481.1 |
CCDSi | CCDS14172.1 |
RefSeqi | NP_005080.1, NM_005089.3 |
Genome annotation databases
Ensembli | ENST00000307771; ENSP00000303015; ENSG00000169249 |
GeneIDi | 8233 |
KEGGi | hsa:8233 |
UCSCi | uc004cxg.5, human |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D49677 mRNA Translation: BAA08533.1 AC004106 Genomic DNA No translation available. AC096510 Genomic DNA No translation available. BC113454 mRNA Translation: AAI13455.1 BC113480 mRNA Translation: AAI13481.1 |
CCDSi | CCDS14172.1 |
RefSeqi | NP_005080.1, NM_005089.3 |
3D structure databases
SMRi | Q15696 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 113865, 57 interactors |
CORUMi | Q15696 |
DIPi | DIP-62117N |
IntActi | Q15696, 51 interactors |
STRINGi | 9606.ENSP00000303015 |
PTM databases
iPTMneti | Q15696 |
PhosphoSitePlusi | Q15696 |
Genetic variation databases
BioMutai | ZRSR2 |
DMDMi | 2833266 |
Proteomic databases
EPDi | Q15696 |
jPOSTi | Q15696 |
MassIVEi | Q15696 |
MaxQBi | Q15696 |
PaxDbi | Q15696 |
PeptideAtlasi | Q15696 |
PRIDEi | Q15696 |
ProteomicsDBi | 60704 |
Protocols and materials databases
Antibodypediai | 8950, 106 antibodies |
DNASUi | 8233 |
Genome annotation databases
Ensembli | ENST00000307771; ENSP00000303015; ENSG00000169249 |
GeneIDi | 8233 |
KEGGi | hsa:8233 |
UCSCi | uc004cxg.5, human |
Organism-specific databases
CTDi | 8233 |
DisGeNETi | 8233 |
GeneCardsi | ZRSR2 |
HGNCi | HGNC:23019, ZRSR2 |
HPAi | ENSG00000169249, Low tissue specificity |
MIMi | 300028, gene |
neXtProti | NX_Q15696 |
OpenTargetsi | ENSG00000169249 |
PharmGKBi | PA162410930 |
VEuPathDBi | HostDB:ENSG00000169249.12 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG2202, Eukaryota |
GeneTreei | ENSGT00950000183152 |
HOGENOMi | CLU_029117_1_0_1 |
InParanoidi | Q15696 |
OMAi | FDTEEQC |
OrthoDBi | 1340384at2759 |
PhylomeDBi | Q15696 |
TreeFami | TF324447 |
Enzyme and pathway databases
PathwayCommonsi | Q15696 |
Reactomei | R-HSA-72165, mRNA Splicing - Minor Pathway |
SIGNORi | Q15696 |
Miscellaneous databases
BioGRID-ORCSi | 8233, 158 hits in 509 CRISPR screens |
ChiTaRSi | ZRSR2, human |
GeneWikii | ZRSR2 |
GenomeRNAii | 8233 |
Pharosi | Q15696, Tbio |
PROi | PR:Q15696 |
RNActi | Q15696, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000169249, Expressed in sural nerve and 237 other tissues |
ExpressionAtlasi | Q15696, baseline and differential |
Genevisiblei | Q15696, HS |
Family and domain databases
Gene3Di | 3.30.70.330, 1 hit |
InterProi | View protein in InterPro IPR012677, Nucleotide-bd_a/b_plait_sf IPR035979, RBD_domain_sf IPR000504, RRM_dom IPR003954, RRM_dom_euk IPR009145, U2AF_small IPR000571, Znf_CCCH |
PANTHERi | PTHR12620, PTHR12620, 1 hit |
Pfami | View protein in Pfam PF00076, RRM_1, 1 hit PF00642, zf-CCCH, 2 hits |
PRINTSi | PR01848, U2AUXFACTOR |
SMARTi | View protein in SMART SM00361, RRM_1, 1 hit SM00356, ZnF_C3H1, 2 hits |
SUPFAMi | SSF54928, SSF54928, 1 hit |
PROSITEi | View protein in PROSITE PS50102, RRM, 1 hit PS50103, ZF_C3H1, 2 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | U2AFM_HUMAN | |
Accessioni | Q15696Primary (citable) accession number: Q15696 Secondary accession number(s): Q14D69 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | May 1, 1997 | |
Last modified: | February 10, 2021 | |
This is version 181 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human chromosome X
Human chromosome X: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot