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Protein

Cdc42-interacting protein 4

Gene

TRIP10

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Required for translocation of GLUT4 to the plasma membrane in response to insulin signaling (By similarity). Required to coordinate membrane tubulation with reorganization of the actin cytoskeleton during endocytosis. Binds to lipids such as phosphatidylinositol 4,5-bisphosphate and phosphatidylserine and promotes membrane invagination and the formation of tubules. Also promotes CDC42-induced actin polymerization by recruiting WASL/N-WASP which in turn activates the Arp2/3 complex. Actin polymerization may promote the fission of membrane tubules to form endocytic vesicles. Required for the formation of podosomes, actin-rich adhesion structures specific to monocyte-derived cells. May be required for the lysosomal retention of FASLG/FASL.By similarity3 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei166Mediates end-to-end attachment of dimers1

GO - Molecular functioni

  • GTPase activator activity Source: Reactome
  • identical protein binding Source: IntAct
  • lipid binding Source: UniProtKB-KW

GO - Biological processi

  • actin cytoskeleton organization Source: UniProtKB
  • cell communication Source: UniProtKB
  • endocytosis Source: UniProtKB-KW
  • membrane organization Source: Reactome
  • regulation of small GTPase mediated signal transduction Source: Reactome
  • signal transduction Source: ProtInc

Keywordsi

Biological processEndocytosis
LigandLipid-binding

Enzyme and pathway databases

ReactomeiR-HSA-194840 Rho GTPase cycle
R-HSA-8856828 Clathrin-mediated endocytosis
SignaLinkiQ15642
SIGNORiQ15642

Names & Taxonomyi

Protein namesi
Recommended name:
Cdc42-interacting protein 4
Alternative name(s):
Protein Felic
Salt tolerant protein
Short name:
hSTP
Thyroid receptor-interacting protein 10
Short name:
TR-interacting protein 10
Short name:
TRIP-10
Gene namesi
Name:TRIP10
Synonyms:CIP4, STOT, STP
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 19

Organism-specific databases

EuPathDBiHostDB:ENSG00000125733.17
HGNCiHGNC:12304 TRIP10
MIMi604504 gene
neXtProtiNX_Q15642

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Cell projection, Cytoplasm, Cytoskeleton, Golgi apparatus, Lysosome, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi454I → S: Abrogates interaction with CDC42. 1 Publication1
Mutagenesisi468L → S: Impairs interaction with CDC42. 1 Publication1

Organism-specific databases

DisGeNETi9322
OpenTargetsiENSG00000125733
PharmGKBiPA36983

Polymorphism and mutation databases

DMDMi118572632

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000897661 – 601Cdc42-interacting protein 4Add BLAST601

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei296PhosphoserineCombined sources1
Modified residuei298PhosphoserineCombined sources1
Modified residuei299PhosphoserineCombined sources1
Modified residuei335PhosphoserineCombined sources1
Modified residuei351PhosphoserineCombined sources1
Modified residuei482PhosphoserineCombined sources1

Post-translational modificationi

Tyrosine phosphorylated. Also phosphorylated by PKA.3 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ15642
MaxQBiQ15642
PeptideAtlasiQ15642
PRIDEiQ15642
ProteomicsDBi60675
60676 [Q15642-2]
60677 [Q15642-3]
60678 [Q15642-4]
60679 [Q15642-5]

PTM databases

iPTMnetiQ15642
PhosphoSitePlusiQ15642

Expressioni

Tissue specificityi

Expressed in brain, colon, heart, kidney, liver, lung, megakaryocyte, ovary, pancreas, peripheral blood lymphocytes, placenta, prostate, skeletal muscle, small intestine, spleen, testis, thymus and trachea.5 Publications

Inductioni

Induced by adriamycin treatment and this effect is counteracted by HGF/SF. Expression is reduced during differentiation.2 Publications

Gene expression databases

BgeeiENSG00000125733 Expressed in 203 organ(s), highest expression level in lower esophagus mucosa
ExpressionAtlasiQ15642 baseline and differential
GenevisibleiQ15642 HS

Organism-specific databases

HPAiHPA041934
HPA072625
HPA073886

Interactioni

Subunit structurei

Interacts specifically with GTP-bound RHOQ. Interacts with DNM2 and PDE6G (By similarity). Homodimerizes, the dimers can polymerize end-to-end to form filamentous structures. Interacts specifically with GTP-bound CDC42. Interacts with AKAP9, ARHGAP17, DAAM1, DIAPH1, DIAPH2, DNM1, FASLG/FASL, GAPVD1, LYN, microtubules, SRC, WAS/WASP and WASL/N-WASP. Interacts with the ligand binding domain of the thyroid receptor (TR) in the presence of thyroid hormone. May interact with CTNNB1 and HD/HTT.By similarity11 Publications

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi114733, 52 interactors
DIPiDIP-39840N
IntActiQ15642, 43 interactors
MINTiQ15642

Structurei

Secondary structure

1601
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ15642
SMRiQ15642
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ15642

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 264F-BARPROSITE-ProRule annotationAdd BLAST264
Domaini393 – 470REM-1PROSITE-ProRule annotationAdd BLAST78
Domaini540 – 601SH3PROSITE-ProRule annotationAdd BLAST62

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 117Required for podosome formation and interaction with AKAP9 and microtubules1 PublicationAdd BLAST117
Regioni1 – 117Required for translocation to the plasma membrane in response to insulinBy similarityAdd BLAST117
Regioni293 – 601Interaction with PDE6GBy similarityAdd BLAST309
Regioni293 – 537Interaction with CDC42Add BLAST245
Regioni471 – 601Required for interaction with FASLG and localization to lysosomes1 PublicationAdd BLAST131
Regioni487 – 541Interaction with DNM2 and WASLBy similarityAdd BLAST55
Regioni529 – 601Interaction with DNM1 and WASL1 PublicationAdd BLAST73
Regioni538 – 601Required for podosome formationAdd BLAST64
Regioni544 – 601Interaction with WAS1 PublicationAdd BLAST58
Regioni546 – 601Interaction with ARHGAP17, DAAM1, DIAPH1 and DIAPH22 PublicationsAdd BLAST56

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili67 – 259Add BLAST193
Coiled coili388 – 481Add BLAST94

Domaini

The F-BAR domain binds the phospholipid membrane with its concave surface. The end-to-end polymerization of dimers of these domains provides a curved surface that fits best membranes with around 600 A diameter, and may drive tubulation.1 Publication

Sequence similaritiesi

Belongs to the FNBP1 family.Curated

Keywords - Domaini

Coiled coil, SH3 domain

Phylogenomic databases

GeneTreeiENSGT00510000046403
HOVERGENiHBG002489
InParanoidiQ15642
KOiK07196
OMAiPTVTEDF
OrthoDBiEOG091G038P
PhylomeDBiQ15642
TreeFamiTF351162

Family and domain databases

Gene3Di1.20.1270.60, 1 hit
InterProiView protein in InterPro
IPR027267 AH/BAR_dom_sf
IPR028498 CIP4
IPR031160 F_BAR
IPR001060 FCH_dom
IPR036028 SH3-like_dom_sf
IPR001452 SH3_domain
PANTHERiPTHR43990:SF2 PTHR43990:SF2, 1 hit
PfamiView protein in Pfam
PF00611 FCH, 1 hit
PF00018 SH3_1, 1 hit
SMARTiView protein in SMART
SM00055 FCH, 1 hit
SM00326 SH3, 1 hit
SUPFAMiSSF103657 SSF103657, 1 hit
SSF50044 SSF50044, 1 hit
PROSITEiView protein in PROSITE
PS51741 F_BAR, 1 hit
PS51860 REM_1, 1 hit
PS50002 SH3, 1 hit

Sequences (5+)i

Sequence statusi: Complete.

This entry describes 5 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 5 described isoforms and 4 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q15642-1) [UniParc]FASTAAdd to basket
Also known as: L

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MDWGTELWDQ FEVLERHTQW GLDLLDRYVK FVKERTEVEQ AYAKQLRSLV
60 70 80 90 100
KKYLPKRPAK DDPESKFSQQ QSFVQILQEV NDFAGQRELV AENLSVRVCL
110 120 130 140 150
ELTKYSQEMK QERKMHFQEG RRAQQQLENG FKQLENSKRK FERDCREAEK
160 170 180 190 200
AAQTAERLDQ DINATKADVE KAKQQAHLRS HMAEESKNEY AAQLQRFNRD
210 220 230 240 250
QAHFYFSQMP QIFDKLQDMD ERRATRLGAG YGLLSEAELE VVPIIAKCLE
260 270 280 290 300
GMKVAANAVD PKNDSHVLIE LHKSGFARPG DVEFEDFSQP MNRAPSDSSL
310 320 330 340 350
GTPSDGRPEL RGPGRSRTKR WPFGKKNKPR PPPLSPLGGP VPSALPNGPP
360 370 380 390 400
SPRSGRDPLA ILSEISKSVK PRLASFRSLR GSRGTVVTED FSHLPPEQQR
410 420 430 440 450
KRLQQQLEER SRELQKEVDQ REALKKMKDV YEKTPQMGDP ASLEPQIAET
460 470 480 490 500
LSNIERLKLE VQKYEAWLAE AESRVLSNRG DSLSRHARPP DPPASAPPDS
510 520 530 540 550
SSNSASQDTK ESSEEPPSEE SQDTPIYTEF DEDFEEEPTS PIGHCVAIYH
560 570 580 590 600
FEGSSEGTIS MAEGEDLSLM EEDKGDGWTR VRRKEGGEGY VPTSYLRVTL

N
Length:601
Mass (Da):68,352
Last modified:November 28, 2006 - v3
Checksum:iA9BFE85520C7ABC5
GO
Isoform 2 (identifier: Q15642-2) [UniParc]FASTAAdd to basket
Also known as: A, W

The sequence of this isoform differs from the canonical sequence as follows:
     329-384: Missing.

Show »
Length:545
Mass (Da):62,592
Checksum:i9C9D72EA734BC6E2
GO
Isoform 3 (identifier: Q15642-3) [UniParc]FASTAAdd to basket
Also known as: B

The sequence of this isoform differs from the canonical sequence as follows:
     329-384: Missing.
     553-601: GSSEGTISMA...PTSYLRVTLN → DLGPPPPPSQ...LTPWLRLRPV

Note: No experimental confirmation available.
Show »
Length:593
Mass (Da):67,603
Checksum:i888A0FE78B42428B
GO
Isoform 4 (identifier: Q15642-4) [UniParc]FASTAAdd to basket
Also known as: C

The sequence of this isoform differs from the canonical sequence as follows:
     329-384: Missing.
     512-512: S → R
     513-601: Missing.

Note: No experimental confirmation available.
Show »
Length:456
Mass (Da):52,700
Checksum:iDBB2D486BC96832A
GO
Isoform 5 (identifier: Q15642-5) [UniParc]FASTAAdd to basket
Also known as: V

The sequence of this isoform differs from the canonical sequence as follows:
     330-341: RPPPLSPLGGPV → SRQPWDSGDRGF
     342-601: Missing.

Note: No experimental confirmation available.
Show »
Length:341
Mass (Da):39,583
Checksum:iA9568C38795E2E1E
GO

Computationally mapped potential isoform sequencesi

There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
W4VSQ9W4VSQ9_HUMAN
Cdc42-interacting protein 4
TRIP10 hCG_23056
593Annotation score:
M0R0F9M0R0F9_HUMAN
Cdc42-interacting protein 4
TRIP10
437Annotation score:
M0R2H7M0R2H7_HUMAN
Cdc42-interacting protein 4
TRIP10
343Annotation score:
M0R070M0R070_HUMAN
Cdc42-interacting protein 4
TRIP10
83Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti158L → P in AAK77492 (PubMed:12054674).Curated1
Sequence conflicti310L → P in AAK77492 (PubMed:12054674).Curated1
Sequence conflicti419D → G in BAD96829 (Ref. 8) Curated1
Sequence conflicti440P → S in BAD96829 (Ref. 8) Curated1
Sequence conflicti473S → R in AAK77492 (PubMed:12054674).Curated1
Sequence conflicti487 – 499ARPPD…SAPPD → KHPIICRLIHFSN in AAC41729 (Ref. 10) CuratedAdd BLAST13
Sequence conflicti553G → W in CAG38751 (Ref. 5) Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_021716329 – 384Missing in isoform 2, isoform 3 and isoform 4. 7 PublicationsAdd BLAST56
Alternative sequenceiVSP_021717330 – 341RPPPL…LGGPV → SRQPWDSGDRGF in isoform 5. 1 PublicationAdd BLAST12
Alternative sequenceiVSP_021718342 – 601Missing in isoform 5. 1 PublicationAdd BLAST260
Alternative sequenceiVSP_021719512S → R in isoform 4. 1 Publication1
Alternative sequenceiVSP_021720513 – 601Missing in isoform 4. 1 PublicationAdd BLAST89
Alternative sequenceiVSP_021721553 – 601GSSEG…RVTLN → DLGPPPPPSQGPARALSLWP RVKTSVLWKKTKGTAGPGSG GKREARATCPPPTSESRSIE PCQRREEGGCRLLLLGHGGS QDLGTLFLTPWLRLRPV in isoform 3. 1 PublicationAdd BLAST49

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ000414 mRNA Translation: CAA04062.1
AF380114 mRNA Translation: AAK77492.1
AY081141 mRNA Translation: AAL89588.1
AB072596 mRNA Translation: BAB88853.1
AF502289 mRNA Translation: AAM46851.1
CR536513 mRNA Translation: CAG38751.1
BT006698 mRNA Translation: AAP35344.1
BT020167 mRNA Translation: AAV38969.1
BT020171 mRNA Translation: AAV43773.1
AK223109 mRNA Translation: BAD96829.1
AK313296 mRNA Translation: BAG36103.1
AC008760 Genomic DNA No translation available.
CH471139 Genomic DNA Translation: EAW69065.1
CH471139 Genomic DNA Translation: EAW69062.1
CH471139 Genomic DNA Translation: EAW69063.1
BC013002 mRNA Translation: AAH13002.1
L40379 mRNA Translation: AAC41729.1
CCDSiCCDS12172.1 [Q15642-2]
CCDS74271.1 [Q15642-1]
CCDS74272.1 [Q15642-3]
RefSeqiNP_001275891.1, NM_001288962.1 [Q15642-1]
NP_001275892.1, NM_001288963.1
NP_004231.1, NM_004240.3 [Q15642-2]
UniGeneiHs.515094

Genome annotation databases

EnsembliENST00000313244; ENSP00000320117; ENSG00000125733 [Q15642-1]
ENST00000313285; ENSP00000320493; ENSG00000125733 [Q15642-2]
GeneIDi9322
KEGGihsa:9322
UCSCiuc002mfr.5 human [Q15642-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ000414 mRNA Translation: CAA04062.1
AF380114 mRNA Translation: AAK77492.1
AY081141 mRNA Translation: AAL89588.1
AB072596 mRNA Translation: BAB88853.1
AF502289 mRNA Translation: AAM46851.1
CR536513 mRNA Translation: CAG38751.1
BT006698 mRNA Translation: AAP35344.1
BT020167 mRNA Translation: AAV38969.1
BT020171 mRNA Translation: AAV43773.1
AK223109 mRNA Translation: BAD96829.1
AK313296 mRNA Translation: BAG36103.1
AC008760 Genomic DNA No translation available.
CH471139 Genomic DNA Translation: EAW69065.1
CH471139 Genomic DNA Translation: EAW69062.1
CH471139 Genomic DNA Translation: EAW69063.1
BC013002 mRNA Translation: AAH13002.1
L40379 mRNA Translation: AAC41729.1
CCDSiCCDS12172.1 [Q15642-2]
CCDS74271.1 [Q15642-1]
CCDS74272.1 [Q15642-3]
RefSeqiNP_001275891.1, NM_001288962.1 [Q15642-1]
NP_001275892.1, NM_001288963.1
NP_004231.1, NM_004240.3 [Q15642-2]
UniGeneiHs.515094

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2CT4NMR-A543-599[»]
2EFKX-ray2.30A10-303[»]
2KE4NMR-A388-481[»]
ProteinModelPortaliQ15642
SMRiQ15642
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi114733, 52 interactors
DIPiDIP-39840N
IntActiQ15642, 43 interactors
MINTiQ15642

PTM databases

iPTMnetiQ15642
PhosphoSitePlusiQ15642

Polymorphism and mutation databases

DMDMi118572632

Proteomic databases

EPDiQ15642
MaxQBiQ15642
PeptideAtlasiQ15642
PRIDEiQ15642
ProteomicsDBi60675
60676 [Q15642-2]
60677 [Q15642-3]
60678 [Q15642-4]
60679 [Q15642-5]

Protocols and materials databases

DNASUi9322
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000313244; ENSP00000320117; ENSG00000125733 [Q15642-1]
ENST00000313285; ENSP00000320493; ENSG00000125733 [Q15642-2]
GeneIDi9322
KEGGihsa:9322
UCSCiuc002mfr.5 human [Q15642-1]

Organism-specific databases

CTDi9322
DisGeNETi9322
EuPathDBiHostDB:ENSG00000125733.17
GeneCardsiTRIP10
H-InvDBiHIX0014704
HGNCiHGNC:12304 TRIP10
HPAiHPA041934
HPA072625
HPA073886
MIMi604504 gene
neXtProtiNX_Q15642
OpenTargetsiENSG00000125733
PharmGKBiPA36983
GenAtlasiSearch...

Phylogenomic databases

GeneTreeiENSGT00510000046403
HOVERGENiHBG002489
InParanoidiQ15642
KOiK07196
OMAiPTVTEDF
OrthoDBiEOG091G038P
PhylomeDBiQ15642
TreeFamiTF351162

Enzyme and pathway databases

ReactomeiR-HSA-194840 Rho GTPase cycle
R-HSA-8856828 Clathrin-mediated endocytosis
SignaLinkiQ15642
SIGNORiQ15642

Miscellaneous databases

ChiTaRSiTRIP10 human
EvolutionaryTraceiQ15642
GeneWikiiTRIP10
GenomeRNAii9322
PROiPR:Q15642
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000125733 Expressed in 203 organ(s), highest expression level in lower esophagus mucosa
ExpressionAtlasiQ15642 baseline and differential
GenevisibleiQ15642 HS

Family and domain databases

Gene3Di1.20.1270.60, 1 hit
InterProiView protein in InterPro
IPR027267 AH/BAR_dom_sf
IPR028498 CIP4
IPR031160 F_BAR
IPR001060 FCH_dom
IPR036028 SH3-like_dom_sf
IPR001452 SH3_domain
PANTHERiPTHR43990:SF2 PTHR43990:SF2, 1 hit
PfamiView protein in Pfam
PF00611 FCH, 1 hit
PF00018 SH3_1, 1 hit
SMARTiView protein in SMART
SM00055 FCH, 1 hit
SM00326 SH3, 1 hit
SUPFAMiSSF103657 SSF103657, 1 hit
SSF50044 SSF50044, 1 hit
PROSITEiView protein in PROSITE
PS51741 F_BAR, 1 hit
PS51860 REM_1, 1 hit
PS50002 SH3, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiCIP4_HUMAN
AccessioniPrimary (citable) accession number: Q15642
Secondary accession number(s): B2R8A6
, B7WP22, D6W645, O15184, Q53G22, Q5TZN1, Q6FI24, Q8NFL1, Q8TCY1, Q8TDX3, Q96RJ1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 28, 2006
Last modified: November 7, 2018
This is version 183 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
  4. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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