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Protein

RISC-loading complex subunit TARBP2

Gene

TARBP2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Required for formation of the RNA induced silencing complex (RISC). Component of the RISC loading complex (RLC), also known as the micro-RNA (miRNA) loading complex (miRLC), which is composed of DICER1, AGO2 and TARBP2. Within the RLC/miRLC, DICER1 and TARBP2 are required to process precursor miRNAs (pre-miRNAs) to mature miRNAs and then load them onto AGO2. AGO2 bound to the mature miRNA constitutes the minimal RISC and may subsequently dissociate from DICER1 and TARBP2. May also play a role in the production of short interfering RNAs (siRNAs) from double-stranded RNA (dsRNA) by DICER1. Binds to the HIV-1 TAR RNA which is located in the long terminal repeat (LTR) of HIV-1, and stimulates translation of TAR-containing RNAs. This is achieved in part at least by binding to and inhibiting EIF2AK2/PKR, thereby reducing phosphorylation and inhibition of EIF2S1/eIF-2-alpha. May also promote translation of TAR-containing RNAs independently of EIF2AK2/PKR.UniRule annotation9 Publications

GO - Molecular functioni

  • double-stranded RNA binding Source: BHF-UCL
  • enzyme binding Source: BHF-UCL
  • identical protein binding Source: IntAct
  • miRNA binding Source: UniProtKB-UniRule
  • pre-miRNA binding Source: BHF-UCL
  • pre-mRNA binding Source: BHF-UCL
  • protein homodimerization activity Source: UniProtKB
  • protein N-terminus binding Source: BHF-UCL
  • siRNA binding Source: UniProtKB

GO - Biological processi

Keywordsi

Molecular functionRNA-binding
Biological processRNA-mediated gene silencing, Translation regulation

Enzyme and pathway databases

ReactomeiR-HSA-203927 MicroRNA (miRNA) biogenesis
R-HSA-426486 Small interfering RNA (siRNA) biogenesis
SIGNORiQ15633

Names & Taxonomyi

Protein namesi
Recommended name:
RISC-loading complex subunit TARBP2UniRule annotation
Alternative name(s):
TAR RNA-binding protein 2
Trans-activation-responsive RNA-binding protein
Gene namesi
Name:TARBP2UniRule annotation
Synonyms:TRBP
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 12

Organism-specific databases

EuPathDBiHostDB:ENSG00000139546.10
HGNCiHGNC:11569 TARBP2
MIMi605053 gene
neXtProtiNX_Q15633

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi6895
OpenTargetsiENSG00000139546
PharmGKBiPA36334

Polymorphism and mutation databases

BioMutaiTARBP2
DMDMi209572714

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000656221 – 366RISC-loading complex subunit TARBP2Add BLAST366

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei152PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ15633
MaxQBiQ15633
PaxDbiQ15633
PeptideAtlasiQ15633
PRIDEiQ15633
ProteomicsDBi60666
60667 [Q15633-2]

PTM databases

iPTMnetiQ15633
PhosphoSitePlusiQ15633

Expressioni

Gene expression databases

BgeeiENSG00000139546 Expressed in 170 organ(s), highest expression level in right hemisphere of cerebellum
CleanExiHS_TARBP2
ExpressionAtlasiQ15633 baseline and differential
GenevisibleiQ15633 HS

Organism-specific databases

HPAiCAB069406
HPA051181
HPA061454

Interactioni

Subunit structurei

Self-associates. Component of the RISC loading complex (RLC), or micro-RNA (miRNA) loading complex (miRLC), which is composed of DICER1, AGO2 and TARBP2. Note that the trimeric RLC/miRLC is also referred to as RISC. Interacts with EIF2AK2/PKR and inhibits its protein kinase activity. Interacts with DHX9 and PRKRA. Interacts with DICER1, AGO2, MOV10, EIF6 and RPL7A (60S ribosome subunit); they form a large RNA-induced silencing complex (RISC) (PubMed:17507929).UniRule annotation11 Publications

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi112758, 79 interactors
ComplexPortaliCPX-134 RISC-loading complex, TARBP2 variant
CORUMiQ15633
DIPiDIP-29665N
IntActiQ15633, 34 interactors
MINTiQ15633
STRINGi9606.ENSP00000266987

Structurei

Secondary structure

1366
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ15633
SMRiQ15633
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ15633

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini30 – 97DRBM 1UniRule annotationAdd BLAST68
Domaini159 – 227DRBM 2UniRule annotationAdd BLAST69
Domaini293 – 361DRBM 3UniRule annotationAdd BLAST69

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni22 – 105Sufficient for interaction with PRKRAAdd BLAST84
Regioni152 – 234Sufficient for interaction with PRKRAAdd BLAST83
Regioni228 – 366Sufficient for interaction with DICER1Add BLAST139
Regioni287 – 366Sufficient for interaction with PRKRAAdd BLAST80

Sequence similaritiesi

Belongs to the TARBP2 family.UniRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG3732 Eukaryota
ENOG410XSCK LUCA
GeneTreeiENSGT00900000141030
HOGENOMiHOG000231919
HOVERGENiHBG001700
InParanoidiQ15633
KOiK18420
OMAiQPITVCH
OrthoDBiEOG091G0I2L
PhylomeDBiQ15633
TreeFamiTF315953

Family and domain databases

CDDicd00048 DSRM, 2 hits
HAMAPiMF_03034 TRBP2, 1 hit
InterProiView protein in InterPro
IPR014720 dsRBD_dom
IPR028605 TRBP2
PANTHERiPTHR10910:SF61 PTHR10910:SF61, 1 hit
PfamiView protein in Pfam
PF00035 dsrm, 2 hits
SMARTiView protein in SMART
SM00358 DSRM, 3 hits
PROSITEiView protein in PROSITE
PS50137 DS_RBD, 3 hits

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative promoter usage. AlignAdd to basket

This entry has 2 described isoforms and 9 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q15633-1) [UniParc]FASTAAdd to basket
Also known as: TRBP2

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MSEEEQGSGT TTGCGLPSIE QMLAANPGKT PISLLQEYGT RIGKTPVYDL
60 70 80 90 100
LKAEGQAHQP NFTFRVTVGD TSCTGQGPSK KAAKHKAAEV ALKHLKGGSM
110 120 130 140 150
LEPALEDSSS FSPLDSSLPE DIPVFTAAAA ATPVPSVVLT RSPPMELQPP
160 170 180 190 200
VSPQQSECNP VGALQELVVQ KGWRLPEYTV TQESGPAHRK EFTMTCRVER
210 220 230 240 250
FIEIGSGTSK KLAKRNAAAK MLLRVHTVPL DARDGNEVEP DDDHFSIGVG
260 270 280 290 300
SRLDGLRNRG PGCTWDSLRN SVGEKILSLR SCSLGSLGAL GPACCRVLSE
310 320 330 340 350
LSEEQAFHVS YLDIEELSLS GLCQCLVELS TQPATVCHGS ATTREAARGE
360
AARRALQYLK IMAGSK
Length:366
Mass (Da):39,039
Last modified:October 14, 2008 - v3
Checksum:i768BA11751DA4912
GO
Isoform 2 (identifier: Q15633-2) [UniParc]FASTAAdd to basket
Also known as: TRBP1

The sequence of this isoform differs from the canonical sequence as follows:
     1-21: Missing.

Show »
Length:345
Mass (Da):36,916
Checksum:i7506A9324E9D0596
GO

Computationally mapped potential isoform sequencesi

There are 9 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
F8VZ57F8VZ57_HUMAN
RISC-loading complex subunit TARBP2
TARBP2
227Annotation score:
F8VP94F8VP94_HUMAN
RISC-loading complex subunit TARBP2
TARBP2
113Annotation score:
F8VYK3F8VYK3_HUMAN
RISC-loading complex subunit TARBP2
TARBP2
187Annotation score:
F8VZZ7F8VZZ7_HUMAN
RISC-loading complex subunit TARBP2
TARBP2
92Annotation score:
F8VYK6F8VYK6_HUMAN
RISC-loading complex subunit TARBP2
TARBP2
88Annotation score:
F8VW32F8VW32_HUMAN
RISC-loading complex subunit TARBP2
TARBP2
165Annotation score:
F8VTT7F8VTT7_HUMAN
RISC-loading complex subunit TARBP2
TARBP2
94Annotation score:
H3BV98H3BV98_HUMAN
RISC-loading complex subunit TARBP2
TARBP2
160Annotation score:
F8VSA1F8VSA1_HUMAN
RISC-loading complex subunit TARBP2
TARBP2
196Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti144P → A in AAA36765 (PubMed:2011739).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_046992251S → F2 PublicationsCorresponds to variant dbSNP:rs1126500Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0355841 – 21Missing in isoform 2. 1 PublicationAdd BLAST21

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M60801 mRNA Translation: AAA36765.1
U08998 mRNA Translation: AAB50581.2
BT007140 mRNA Translation: AAP35804.1
CH471054 Genomic DNA Translation: EAW96720.1
BC005860 mRNA Translation: AAH05860.1
AF281068 Genomic DNA Translation: AAL55730.1
AF281068 Genomic DNA Translation: AAL55731.1
CCDSiCCDS41791.1 [Q15633-2]
CCDS8861.1 [Q15633-1]
PIRiG01420
RefSeqiNP_004169.3, NM_004178.4 [Q15633-2]
NP_599150.1, NM_134323.1 [Q15633-1]
NP_599151.2, NM_134324.2 [Q15633-2]
XP_005269171.1, XM_005269114.1 [Q15633-2]
XP_005269172.1, XM_005269115.2 [Q15633-2]
UniGeneiHs.326
Hs.604255

Genome annotation databases

EnsembliENST00000266987; ENSP00000266987; ENSG00000139546 [Q15633-1]
ENST00000394357; ENSP00000377885; ENSG00000139546 [Q15633-2]
ENST00000456234; ENSP00000416077; ENSG00000139546 [Q15633-2]
GeneIDi6895
KEGGihsa:6895
UCSCiuc001sdo.4 human [Q15633-1]

Keywords - Coding sequence diversityi

Alternative promoter usage, Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M60801 mRNA Translation: AAA36765.1
U08998 mRNA Translation: AAB50581.2
BT007140 mRNA Translation: AAP35804.1
CH471054 Genomic DNA Translation: EAW96720.1
BC005860 mRNA Translation: AAH05860.1
AF281068 Genomic DNA Translation: AAL55730.1
AF281068 Genomic DNA Translation: AAL55731.1
CCDSiCCDS41791.1 [Q15633-2]
CCDS8861.1 [Q15633-1]
PIRiG01420
RefSeqiNP_004169.3, NM_004178.4 [Q15633-2]
NP_599150.1, NM_134323.1 [Q15633-1]
NP_599151.2, NM_134324.2 [Q15633-2]
XP_005269171.1, XM_005269114.1 [Q15633-2]
XP_005269172.1, XM_005269115.2 [Q15633-2]
UniGeneiHs.326
Hs.604255

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2CPNNMR-A150-225[»]
3ADLX-ray2.20A161-231[»]
3LLHX-ray2.14A/B22-105[»]
4WYQX-ray3.20B/E289-363[»]
5N8LNMR-A16-227[»]
5N8MNMR-A16-227[»]
5ZAKelectron microscopy4.40B1-366[»]
5ZALelectron microscopy4.70B1-366[»]
5ZAMelectron microscopy5.70B1-366[»]
ProteinModelPortaliQ15633
SMRiQ15633
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi112758, 79 interactors
ComplexPortaliCPX-134 RISC-loading complex, TARBP2 variant
CORUMiQ15633
DIPiDIP-29665N
IntActiQ15633, 34 interactors
MINTiQ15633
STRINGi9606.ENSP00000266987

PTM databases

iPTMnetiQ15633
PhosphoSitePlusiQ15633

Polymorphism and mutation databases

BioMutaiTARBP2
DMDMi209572714

Proteomic databases

EPDiQ15633
MaxQBiQ15633
PaxDbiQ15633
PeptideAtlasiQ15633
PRIDEiQ15633
ProteomicsDBi60666
60667 [Q15633-2]

Protocols and materials databases

DNASUi6895
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000266987; ENSP00000266987; ENSG00000139546 [Q15633-1]
ENST00000394357; ENSP00000377885; ENSG00000139546 [Q15633-2]
ENST00000456234; ENSP00000416077; ENSG00000139546 [Q15633-2]
GeneIDi6895
KEGGihsa:6895
UCSCiuc001sdo.4 human [Q15633-1]

Organism-specific databases

CTDi6895
DisGeNETi6895
EuPathDBiHostDB:ENSG00000139546.10
GeneCardsiTARBP2
HGNCiHGNC:11569 TARBP2
HPAiCAB069406
HPA051181
HPA061454
MIMi605053 gene
neXtProtiNX_Q15633
OpenTargetsiENSG00000139546
PharmGKBiPA36334
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3732 Eukaryota
ENOG410XSCK LUCA
GeneTreeiENSGT00900000141030
HOGENOMiHOG000231919
HOVERGENiHBG001700
InParanoidiQ15633
KOiK18420
OMAiQPITVCH
OrthoDBiEOG091G0I2L
PhylomeDBiQ15633
TreeFamiTF315953

Enzyme and pathway databases

ReactomeiR-HSA-203927 MicroRNA (miRNA) biogenesis
R-HSA-426486 Small interfering RNA (siRNA) biogenesis
SIGNORiQ15633

Miscellaneous databases

EvolutionaryTraceiQ15633
GeneWikiiTARBP2
GenomeRNAii6895
PROiPR:Q15633
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000139546 Expressed in 170 organ(s), highest expression level in right hemisphere of cerebellum
CleanExiHS_TARBP2
ExpressionAtlasiQ15633 baseline and differential
GenevisibleiQ15633 HS

Family and domain databases

CDDicd00048 DSRM, 2 hits
HAMAPiMF_03034 TRBP2, 1 hit
InterProiView protein in InterPro
IPR014720 dsRBD_dom
IPR028605 TRBP2
PANTHERiPTHR10910:SF61 PTHR10910:SF61, 1 hit
PfamiView protein in Pfam
PF00035 dsrm, 2 hits
SMARTiView protein in SMART
SM00358 DSRM, 3 hits
PROSITEiView protein in PROSITE
PS50137 DS_RBD, 3 hits
ProtoNetiSearch...

Entry informationi

Entry nameiTRBP2_HUMAN
AccessioniPrimary (citable) accession number: Q15633
Secondary accession number(s): Q12878
, Q8WY32, Q8WY33, Q9BRY2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: October 14, 2008
Last modified: November 7, 2018
This is version 178 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. Human chromosome 12
    Human chromosome 12: entries, gene names and cross-references to MIM
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Main funding by: National Institutes of Health

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