UniProtKB - Q15631 (TSN_HUMAN)
Translin
TSN
Functioni
DNA-binding protein that specifically recognizes consensus sequences at the breakpoint junctions in chromosomal translocations, mostly involving immunoglobulin (Ig)/T-cell receptor gene segments. Seems to recognize single-stranded DNA ends generated by staggered breaks occurring at recombination hot spots.
Exhibits both single-stranded and double-stranded endoribonuclease activity. May act as an activator of RNA-induced silencing complex (RISC) by facilitating endonucleolytic cleavage of the siRNA passenger strand.
GO - Molecular functioni
- DNA binding Source: ProtInc
- endonuclease activity Source: UniProtKB-KW
- identical protein binding Source: IntAct
- mRNA binding Source: Ensembl
- RNA binding Source: GO_Central
- sequence-specific DNA binding Source: InterPro
- single-stranded DNA binding Source: InterPro
GO - Biological processi
- DNA recombination Source: ProtInc
- production of siRNA involved in RNA interference Source: ComplexPortal
- RNA interference Source: ComplexPortal
Keywordsi
Molecular function | DNA-binding, Endonuclease, Hydrolase, Nuclease, RNA-binding |
Enzyme and pathway databases
PathwayCommonsi | Q15631 |
Reactomei | R-HSA-426486, Small interfering RNA (siRNA) biogenesis |
SignaLinki | Q15631 |
Names & Taxonomyi
Protein namesi | Recommended name: Translin (EC:3.1.-.-)Alternative name(s): Component 3 of promoter of RISC Short name: C3PO |
Gene namesi | Name:TSN |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:12379, TSN |
MIMi | 600575, gene |
neXtProti | NX_Q15631 |
VEuPathDBi | HostDB:ENSG00000211460 |
Subcellular locationi
Cytosol
- cytosol Source: Reactome
Endoplasmic reticulum
- endoplasmic reticulum Source: HPA
Nucleus
- nucleoplasm Source: HPA
- nucleus Source: GO_Central
Other locations
- cytoplasm Source: GO_Central
- endoribonuclease complex Source: ComplexPortal
Keywords - Cellular componenti
Cytoplasm, NucleusPathology & Biotechi
Organism-specific databases
DisGeNETi | 7247 |
OpenTargetsi | ENSG00000211460 |
PharmGKBi | PA37047 |
Miscellaneous databases
Pharosi | Q15631, Tbio |
Genetic variation databases
BioMutai | TSN |
DMDMi | 6136060 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000191683 | 1 – 228 | TranslinAdd BLAST | 228 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 187 | N6-acetyllysineCombined sources | 1 | |
Modified residuei | 190 | PhosphoserineCombined sources | 1 | |
Modified residuei | 199 | N6-acetyllysineCombined sources | 1 |
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
EPDi | Q15631 |
jPOSTi | Q15631 |
MassIVEi | Q15631 |
MaxQBi | Q15631 |
PaxDbi | Q15631 |
PeptideAtlasi | Q15631 |
PRIDEi | Q15631 |
ProteomicsDBi | 60665 [Q15631-1] 6554 |
TopDownProteomicsi | Q15631-1 [Q15631-1] |
PTM databases
GlyGeni | Q15631, 1 site, 1 O-linked glycan (1 site) |
iPTMneti | Q15631 |
PhosphoSitePlusi | Q15631 |
Expressioni
Gene expression databases
Bgeei | ENSG00000211460, Expressed in female gonad and 252 other tissues |
ExpressionAtlasi | Q15631, baseline and differential |
Genevisiblei | Q15631, HS |
Organism-specific databases
HPAi | ENSG00000211460, Low tissue specificity |
Interactioni
Subunit structurei
Ring-shaped heterooctamer of six TSN and two TSNAX subunits, DNA/RNA binding occurs inside the ring.
1 PublicationBinary interactionsi
Q15631
GO - Molecular functioni
- identical protein binding Source: IntAct
Protein-protein interaction databases
BioGRIDi | 113098, 56 interactors |
ComplexPortali | CPX-890, C3PO endoribonuclease complex |
DIPi | DIP-42216N |
IntActi | Q15631, 22 interactors |
MINTi | Q15631 |
STRINGi | 9606.ENSP00000374332 |
Miscellaneous databases
RNActi | Q15631, protein |
Structurei
Secondary structure
3D structure databases
AlphaFoldDBi | Q15631 |
SMRi | Q15631 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q15631 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 86 – 90 | DNA/RNA binding | 5 | |
Regioni | 177 – 198 | Leucine-zipperSequence analysisAdd BLAST | 22 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG3067, Eukaryota |
GeneTreei | ENSGT00940000153568 |
HOGENOMi | CLU_079179_0_0_1 |
InParanoidi | Q15631 |
OMAi | LHAGFQI |
OrthoDBi | 1187354at2759 |
PhylomeDBi | Q15631 |
TreeFami | TF323690 |
Family and domain databases
CDDi | cd14819, Translin, 1 hit |
DisProti | DP02701 |
Gene3Di | 1.20.58.190, 1 hit 1.20.58.200, 1 hit |
InterProi | View protein in InterPro IPR033956, Translin IPR016069, Translin_C IPR002848, Translin_fam IPR016068, Translin_N IPR036081, Translin_sf |
PANTHERi | PTHR10741, PTHR10741, 1 hit PTHR10741:SF2, PTHR10741:SF2, 1 hit |
Pfami | View protein in Pfam PF01997, Translin, 1 hit |
SUPFAMi | SSF74784, SSF74784, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 4 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MSVSEIFVEL QGFLAAEQDI REEIRKVVQS LEQTAREILT LLQGVHQGAG
60 70 80 90 100
FQDIPKRCLK AREHFGTVKT HLTSLKTKFP AEQYYRFHEH WRFVLQRLVF
110 120 130 140 150
LAAFVVYLET ETLVTREAVT EILGIEPDRE KGFHLDVEDY LSGVLILASE
160 170 180 190 200
LSRLSVNSVT AGDYSRPLHI STFINELDSG FRLLNLKNDS LRKRYDGLKY
210 220
DVKKVEEVVY DLSIRGFNKE TAAACVEK
Computationally mapped potential isoform sequencesi
There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketE9PGT1 | E9PGT1_HUMAN | Translin | TSN | 223 | Annotation score: | ||
H7C1D4 | H7C1D4_HUMAN | Translin | TSN | 185 | Annotation score: | ||
A0A0J9YVW2 | A0A0J9YVW2_HUMAN | Translin | TSN | 92 | Annotation score: | ||
A0A0J9YX41 | A0A0J9YX41_HUMAN | Translin | TSN | 36 | Annotation score: |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_044937 | 127 – 148 | PDREK…VLILA → AVCQQRDCWRLLPTPPHLHL HQ in isoform 2. 1 PublicationAdd BLAST | 22 | |
Alternative sequenceiVSP_044938 | 149 – 228 | Missing in isoform 2. 1 PublicationAdd BLAST | 80 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X78627 mRNA Translation: CAA55341.1 BT019490 mRNA Translation: AAV38297.1 BT019491 mRNA Translation: AAV38298.1 AK296469 mRNA Translation: BAH12364.1 AC018737 Genomic DNA Translation: AAY14831.1 BC002359 mRNA Translation: AAH02359.1 Y12563 , Y12564, Y12565, Y12566, Y12567 Genomic DNA Translation: CAA73150.1 |
CCDSi | CCDS33284.1 [Q15631-1] CCDS58723.1 [Q15631-2] |
PIRi | S51738 |
RefSeqi | NP_001248330.1, NM_001261401.1 [Q15631-2] NP_004613.1, NM_004622.2 [Q15631-1] |
Genome annotation databases
Ensembli | ENST00000389682.8; ENSP00000374332.3; ENSG00000211460.12 ENST00000536142.5; ENSP00000437728.1; ENSG00000211460.12 [Q15631-2] |
GeneIDi | 7247 |
KEGGi | hsa:7247 |
MANE-Selecti | ENST00000389682.8; ENSP00000374332.3; NM_004622.3; NP_004613.1 |
UCSCi | uc002tnl.4, human [Q15631-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X78627 mRNA Translation: CAA55341.1 BT019490 mRNA Translation: AAV38297.1 BT019491 mRNA Translation: AAV38298.1 AK296469 mRNA Translation: BAH12364.1 AC018737 Genomic DNA Translation: AAY14831.1 BC002359 mRNA Translation: AAH02359.1 Y12563 , Y12564, Y12565, Y12566, Y12567 Genomic DNA Translation: CAA73150.1 |
CCDSi | CCDS33284.1 [Q15631-1] CCDS58723.1 [Q15631-2] |
PIRi | S51738 |
RefSeqi | NP_001248330.1, NM_001261401.1 [Q15631-2] NP_004613.1, NM_004622.2 [Q15631-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1J1J | X-ray | 2.20 | A/B/C/D | 1-228 | [»] | |
3PJA | X-ray | 3.00 | A/B/C/D/E/F/G/H/I | 1-228 | [»] | |
3QB5 | X-ray | 2.95 | A/B/C | 1-228 | [»] | |
4WYV | X-ray | 3.00 | A/B/C/D/E/F/G/H | 1-228 | [»] | |
AlphaFoldDBi | Q15631 | |||||
SMRi | Q15631 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 113098, 56 interactors |
ComplexPortali | CPX-890, C3PO endoribonuclease complex |
DIPi | DIP-42216N |
IntActi | Q15631, 22 interactors |
MINTi | Q15631 |
STRINGi | 9606.ENSP00000374332 |
PTM databases
GlyGeni | Q15631, 1 site, 1 O-linked glycan (1 site) |
iPTMneti | Q15631 |
PhosphoSitePlusi | Q15631 |
Genetic variation databases
BioMutai | TSN |
DMDMi | 6136060 |
Proteomic databases
EPDi | Q15631 |
jPOSTi | Q15631 |
MassIVEi | Q15631 |
MaxQBi | Q15631 |
PaxDbi | Q15631 |
PeptideAtlasi | Q15631 |
PRIDEi | Q15631 |
ProteomicsDBi | 60665 [Q15631-1] 6554 |
TopDownProteomicsi | Q15631-1 [Q15631-1] |
Protocols and materials databases
Antibodypediai | 33410, 224 antibodies from 34 providers |
DNASUi | 7247 |
Genome annotation databases
Ensembli | ENST00000389682.8; ENSP00000374332.3; ENSG00000211460.12 ENST00000536142.5; ENSP00000437728.1; ENSG00000211460.12 [Q15631-2] |
GeneIDi | 7247 |
KEGGi | hsa:7247 |
MANE-Selecti | ENST00000389682.8; ENSP00000374332.3; NM_004622.3; NP_004613.1 |
UCSCi | uc002tnl.4, human [Q15631-1] |
Organism-specific databases
CTDi | 7247 |
DisGeNETi | 7247 |
GeneCardsi | TSN |
HGNCi | HGNC:12379, TSN |
HPAi | ENSG00000211460, Low tissue specificity |
MIMi | 600575, gene |
neXtProti | NX_Q15631 |
OpenTargetsi | ENSG00000211460 |
PharmGKBi | PA37047 |
VEuPathDBi | HostDB:ENSG00000211460 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG3067, Eukaryota |
GeneTreei | ENSGT00940000153568 |
HOGENOMi | CLU_079179_0_0_1 |
InParanoidi | Q15631 |
OMAi | LHAGFQI |
OrthoDBi | 1187354at2759 |
PhylomeDBi | Q15631 |
TreeFami | TF323690 |
Enzyme and pathway databases
PathwayCommonsi | Q15631 |
Reactomei | R-HSA-426486, Small interfering RNA (siRNA) biogenesis |
SignaLinki | Q15631 |
Miscellaneous databases
BioGRID-ORCSi | 7247, 9 hits in 1079 CRISPR screens |
ChiTaRSi | TSN, human |
EvolutionaryTracei | Q15631 |
GeneWikii | TSN_(gene) |
GenomeRNAii | 7247 |
Pharosi | Q15631, Tbio |
PROi | PR:Q15631 |
RNActi | Q15631, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000211460, Expressed in female gonad and 252 other tissues |
ExpressionAtlasi | Q15631, baseline and differential |
Genevisiblei | Q15631, HS |
Family and domain databases
CDDi | cd14819, Translin, 1 hit |
DisProti | DP02701 |
Gene3Di | 1.20.58.190, 1 hit 1.20.58.200, 1 hit |
InterProi | View protein in InterPro IPR033956, Translin IPR016069, Translin_C IPR002848, Translin_fam IPR016068, Translin_N IPR036081, Translin_sf |
PANTHERi | PTHR10741, PTHR10741, 1 hit PTHR10741:SF2, PTHR10741:SF2, 1 hit |
Pfami | View protein in Pfam PF01997, Translin, 1 hit |
SUPFAMi | SSF74784, SSF74784, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | TSN_HUMAN | |
Accessioni | Q15631Primary (citable) accession number: Q15631 Secondary accession number(s): B7Z3X8, Q5U0K7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 15, 1999 |
Last sequence update: | November 1, 1996 | |
Last modified: | May 25, 2022 | |
This is version 185 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 2
Human chromosome 2: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families