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Protein

Dixin

Gene

DIXDC1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Positive effector of the Wnt signaling pathway; activates WNT3A signaling via DVL2. Regulates JNK activation by AXIN1 and DVL2.2 Publications

GO - Molecular functioni

  • actin binding Source: UniProtKB-KW
  • gamma-tubulin binding Source: UniProtKB
  • protein domain specific binding Source: Ensembl

GO - Biological processi

Keywordsi

Molecular functionActin-binding, Developmental protein
Biological processWnt signaling pathway

Names & Taxonomyi

Protein namesi
Recommended name:
Dixin
Alternative name(s):
Coiled-coil protein DIX1
Short name:
Coiled-coil-DIX1
DIX domain-containing protein 1
Gene namesi
Name:DIXDC1
Synonyms:CCD1, KIAA1735
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 11

Organism-specific databases

EuPathDBiHostDB:ENSG00000150764.13
HGNCiHGNC:23695 DIXDC1
MIMi610493 gene
neXtProtiNX_Q155Q3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell junction, Cytoplasm, Cytoskeleton

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi648D → A: Loss of interaction with DVL2. Abolishes activation of Wnt signaling. 1 Publication1
Mutagenesisi651F → A: Loss of interaction with DVL2. Abolishes activation of Wnt signaling. 1 Publication1
Mutagenesisi655K → A: Loss of interaction with DVL2. Abolishes activation of Wnt signaling. 1 Publication1

Organism-specific databases

DisGeNETi85458
OpenTargetsiENSG00000150764
PharmGKBiPA134988674

Polymorphism and mutation databases

DMDMi147641721

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002872231 – 683DixinAdd BLAST683
Isoform 2 (identifier: Q155Q3-2)
Initiator methionineiRemoved

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei186PhosphoserineBy similarity1
Modified residuei231PhosphoserineBy similarity1
Modified residuei590PhosphoserineCombined sources1
Isoform 2 (identifier: Q155Q3-2)
Lipidationi2N-myristoyl glycine1

Post-translational modificationi

Phosphorylated on tyrosine and serine residues.1 Publication
Polyubiquitinated, leading to its proteasomal degradation. WNT3A signaling increases DIXDC1 protein levels by inhibiting its ubiquitination and subsequent degradation.1 Publication

Keywords - PTMi

Lipoprotein, Myristate, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ155Q3
MaxQBiQ155Q3
PaxDbiQ155Q3
PeptideAtlasiQ155Q3
PRIDEiQ155Q3
ProteomicsDBi60652
60653 [Q155Q3-2]
60654 [Q155Q3-4]

PTM databases

iPTMnetiQ155Q3
PhosphoSitePlusiQ155Q3

Expressioni

Tissue specificityi

Ubiquitously expressed with higher expression in cardiac and skeletal muscles.1 Publication

Gene expression databases

BgeeiENSG00000150764 Expressed in 235 organ(s), highest expression level in corpus callosum
CleanExiHS_DIXDC1
ExpressionAtlasiQ155Q3 baseline and differential
GenevisibleiQ155Q3 HS

Organism-specific databases

HPAiHPA039658

Interactioni

Subunit structurei

Isoform 1 but not isoform 2 binds filamentous actin. Interacts with the complex composed of DVL2 and Rac. Interacts with AXIN1; competes with MAP3K1. Interacts with MAP3K4 preventing MAP3K4 interaction with AXIN1. Directly interacts (via DIX domain) with DVL2 (via DIX domain). Interacts with gamma-tubulin.4 Publications

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi124542, 22 interactors
CORUMiQ155Q3
IntActiQ155Q3, 20 interactors
MINTiQ155Q3
STRINGi9606.ENSP00000394352

Structurei

Secondary structure

1683
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ155Q3
SMRiQ155Q3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini20 – 127Calponin-homology (CH)PROSITE-ProRule annotationAdd BLAST108
Domaini600 – 680DIXPROSITE-ProRule annotationAdd BLAST81

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni127 – 300Actin-bindingAdd BLAST174

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili279 – 452Sequence analysisAdd BLAST174

Domaini

The coiled-coil domain mediates interaction with MAP3K4 and inhibition of AXIN1-mediated JNK activation through MAP3K4.1 Publication
The DIX domain mediates interaction with AXIN1 and inhibition of AXIN1-mediated JNK activation through MAP3K1. Mediates interaction with DVL2; this interaction is required for activation of Wnt signaling.1 Publication

Sequence similaritiesi

Belongs to the DIXDC1 family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG410IFNC Eukaryota
ENOG410XPPR LUCA
GeneTreeiENSGT00390000013552
HOVERGENiHBG107812
InParanoidiQ155Q3
OMAiQEMRENV
OrthoDBiEOG091G03RC
PhylomeDBiQ155Q3

Family and domain databases

CDDicd00014 CH, 1 hit
Gene3Di1.10.418.10, 1 hit
3.10.20.380, 1 hit
InterProiView protein in InterPro
IPR001715 CH-domain
IPR036872 CH_dom_sf
IPR001158 DIX
IPR038207 DIX_dom_sf
IPR015506 Dsh/Dvl-rel
IPR029071 Ubiquitin-like_domsf
PANTHERiPTHR10878 PTHR10878, 1 hit
PfamiView protein in Pfam
PF00307 CH, 1 hit
PF00778 DIX, 1 hit
SMARTiView protein in SMART
SM00033 CH, 1 hit
SM00021 DAX, 1 hit
SUPFAMiSSF47576 SSF47576, 1 hit
SSF54236 SSF54236, 1 hit
PROSITEiView protein in PROSITE
PS50021 CH, 1 hit
PS50841 DIX, 1 hit

Sequences (4+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 4 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All

Isoform 1 (identifier: Q155Q3-1) [UniParc]FASTAAdd to basket
Also known as: l-DIXDC1

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MLACLTRGNL LDVLQEGFNE QQLQAYVAWV NAQLKKRPAV KPVQDLRQDL
60 70 80 90 100
RDGVILAYLI EIVAGEKLSG VQLSPGNQQE MKNNVEKVLQ FVASKKIRMH
110 120 130 140 150
QTSAKDIVDG NLKSIMRLVL ALAAHFKPGS SRTVNQGRDS RAPLQSHRPH
160 170 180 190 200
CATAVAQGAA AALADVCHDM SRSGRDVFRY RQRNSSMDEE IENPYWSVRA
210 220 230 240 250
LVQQYEGQQR SPSESSCSSL TSPSPIHSAK SESIITQSEE KADFVIIPAE
260 270 280 290 300
GIENRTEGTD SPLSRDWRPG SPGTYLETSW EEQLLEQQEY LEKEMEEAKK
310 320 330 340 350
MISGLQALLL NGSLPEDEQE RPLALCEPGV NPEEQLIIIQ SRLDQSMEEN
360 370 380 390 400
QDLKKELLKC KQEARNLQGI KDALQQRLTQ QDTSVLQLKQ ELLRANMDKD
410 420 430 440 450
ELHNQNVDLQ RKLDERNRLL GEYKKELGQK DRLLQQHQAK LEEALRKLSD
460 470 480 490 500
VSYHQVDLER ELEHKDVLLA HCMKREADEA TNYNSHNSQS NGFLLPTAGK
510 520 530 540 550
GATSVSNRGT SDLQLVRDAL RSLRNSFSGH DPQHHTIDSL EQGISSLMER
560 570 580 590 600
LHVMETQKKQ ERKVRVKSPR TQVGSEYRES WPPNSKLPHS QSSPTVSSTC
610 620 630 640 650
TKVLYFTDRS LTPFMVNIPK RLEEVTLKDF KAAIDREGNH RYHFKALDPE
660 670 680
FGTVKEEIFH DDDAIPGWEG KIVAWVEEDH GEN
Note: Major isoform. Ubiquitously expressed.
Length:683
Mass (Da):77,478
Last modified:May 15, 2007 - v2
Checksum:iE643D1A69B00238C
GO
Isoform 2 (identifier: Q155Q3-2) [UniParc]FASTAAdd to basket
Also known as: s-DIXDC1

The sequence of this isoform differs from the canonical sequence as follows:
     1-211: Missing.
     212-219: PSESSCSS → MGGTQVKC

Note: Major isoform. Preferentially expressed in cardiac and skeletal muscles.
Show »
Length:472
Mass (Da):53,942
Checksum:i70C4312A59CAAF47
GO
Isoform 3 (identifier: Q155Q3-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     219-219: S → R
     220-683: Missing.

Note: No experimental confirmation available.
Show »
Length:219
Mass (Da):24,428
Checksum:iF27E0386517E1EAB
GO
Isoform 4 (identifier: Q155Q3-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-20: MLACLTRGNLLDVLQEGFNE → MGTQVVMRFNNSLLPTEPS
     219-219: S → R
     220-683: Missing.

Note: No experimental confirmation available.
Show »
Length:218
Mass (Da):24,313
Checksum:i7EC65502524E280C
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A087WVH1A0A087WVH1_HUMAN
Dixin
DIXDC1
77Annotation score:

Sequence cautioni

The sequence AAH35509 differs from that shown. Contaminating sequence. Potential poly-A sequence.Curated
The sequence AAH64479 differs from that shown. Contaminating sequence. Potential poly-A sequence.Curated
The sequence BAB21826 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence BAB71039 differs from that shown. Probable cloning artifact.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti140S → P in ABG25914 (PubMed:16814745).Curated1
Sequence conflicti144L → R in ABG25914 (PubMed:16814745).Curated1
Sequence conflicti168H → Q in ABG25914 (PubMed:16814745).Curated1
Sequence conflicti378L → S in BAB71039 (PubMed:15489334).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_032294300K → R. Corresponds to variant dbSNP:rs34575249Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0253781 – 211Missing in isoform 2. 2 PublicationsAdd BLAST211
Alternative sequenceiVSP_0545651 – 20MLACL…EGFNE → MGTQVVMRFNNSLLPTEPS in isoform 4. 1 PublicationAdd BLAST20
Alternative sequenceiVSP_025379212 – 219PSESSCSS → MGGTQVKC in isoform 2. 2 Publications8
Alternative sequenceiVSP_025380219S → R in isoform 3 and isoform 4. 1 Publication1
Alternative sequenceiVSP_025381220 – 683Missing in isoform 3 and isoform 4. 1 PublicationAdd BLAST464

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ642016 mRNA Translation: ABG25914.1
AB051522 mRNA Translation: BAB21826.1 Different initiation.
AP000907 Genomic DNA No translation available.
CH471065 Genomic DNA Translation: EAW67171.1
BC033034 mRNA Translation: AAH33034.1
BC035509 mRNA Translation: AAH35509.1 Sequence problems.
BC041626 mRNA Translation: AAH41626.2
BC048294 mRNA Translation: AAH48294.1
BC064479 mRNA Translation: AAH64479.1 Sequence problems.
BC128600 mRNA Translation: AAI28601.1
AK055899 mRNA Translation: BAB71039.1 Sequence problems.
CCDSiCCDS60957.1 [Q155Q3-5]
CCDS73381.1 [Q155Q3-1]
CCDS73382.1 [Q155Q3-2]
RefSeqiNP_001033043.1, NM_001037954.3 [Q155Q3-1]
NP_001265471.1, NM_001278542.1 [Q155Q3-5]
NP_219493.1, NM_033425.4 [Q155Q3-2]
UniGeneiHs.655626

Genome annotation databases

EnsembliENST00000440460; ENSP00000394352; ENSG00000150764 [Q155Q3-1]
ENST00000529225; ENSP00000434130; ENSG00000150764 [Q155Q3-5]
ENST00000615255; ENSP00000480808; ENSG00000150764 [Q155Q3-2]
GeneIDi85458
KEGGihsa:85458
UCSCiuc001pmj.4 human [Q155Q3-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ642016 mRNA Translation: ABG25914.1
AB051522 mRNA Translation: BAB21826.1 Different initiation.
AP000907 Genomic DNA No translation available.
CH471065 Genomic DNA Translation: EAW67171.1
BC033034 mRNA Translation: AAH33034.1
BC035509 mRNA Translation: AAH35509.1 Sequence problems.
BC041626 mRNA Translation: AAH41626.2
BC048294 mRNA Translation: AAH48294.1
BC064479 mRNA Translation: AAH64479.1 Sequence problems.
BC128600 mRNA Translation: AAI28601.1
AK055899 mRNA Translation: BAB71039.1 Sequence problems.
CCDSiCCDS60957.1 [Q155Q3-5]
CCDS73381.1 [Q155Q3-1]
CCDS73382.1 [Q155Q3-2]
RefSeqiNP_001033043.1, NM_001037954.3 [Q155Q3-1]
NP_001265471.1, NM_001278542.1 [Q155Q3-5]
NP_219493.1, NM_033425.4 [Q155Q3-2]
UniGeneiHs.655626

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3PZ7X-ray2.44A597-683[»]
ProteinModelPortaliQ155Q3
SMRiQ155Q3
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi124542, 22 interactors
CORUMiQ155Q3
IntActiQ155Q3, 20 interactors
MINTiQ155Q3
STRINGi9606.ENSP00000394352

PTM databases

iPTMnetiQ155Q3
PhosphoSitePlusiQ155Q3

Polymorphism and mutation databases

DMDMi147641721

Proteomic databases

EPDiQ155Q3
MaxQBiQ155Q3
PaxDbiQ155Q3
PeptideAtlasiQ155Q3
PRIDEiQ155Q3
ProteomicsDBi60652
60653 [Q155Q3-2]
60654 [Q155Q3-4]

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000440460; ENSP00000394352; ENSG00000150764 [Q155Q3-1]
ENST00000529225; ENSP00000434130; ENSG00000150764 [Q155Q3-5]
ENST00000615255; ENSP00000480808; ENSG00000150764 [Q155Q3-2]
GeneIDi85458
KEGGihsa:85458
UCSCiuc001pmj.4 human [Q155Q3-1]

Organism-specific databases

CTDi85458
DisGeNETi85458
EuPathDBiHostDB:ENSG00000150764.13
GeneCardsiDIXDC1
HGNCiHGNC:23695 DIXDC1
HPAiHPA039658
MIMi610493 gene
neXtProtiNX_Q155Q3
OpenTargetsiENSG00000150764
PharmGKBiPA134988674
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IFNC Eukaryota
ENOG410XPPR LUCA
GeneTreeiENSGT00390000013552
HOVERGENiHBG107812
InParanoidiQ155Q3
OMAiQEMRENV
OrthoDBiEOG091G03RC
PhylomeDBiQ155Q3

Miscellaneous databases

ChiTaRSiDIXDC1 human
GeneWikiiDIXDC1
GenomeRNAii85458
PROiPR:Q155Q3
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000150764 Expressed in 235 organ(s), highest expression level in corpus callosum
CleanExiHS_DIXDC1
ExpressionAtlasiQ155Q3 baseline and differential
GenevisibleiQ155Q3 HS

Family and domain databases

CDDicd00014 CH, 1 hit
Gene3Di1.10.418.10, 1 hit
3.10.20.380, 1 hit
InterProiView protein in InterPro
IPR001715 CH-domain
IPR036872 CH_dom_sf
IPR001158 DIX
IPR038207 DIX_dom_sf
IPR015506 Dsh/Dvl-rel
IPR029071 Ubiquitin-like_domsf
PANTHERiPTHR10878 PTHR10878, 1 hit
PfamiView protein in Pfam
PF00307 CH, 1 hit
PF00778 DIX, 1 hit
SMARTiView protein in SMART
SM00033 CH, 1 hit
SM00021 DAX, 1 hit
SUPFAMiSSF47576 SSF47576, 1 hit
SSF54236 SSF54236, 1 hit
PROSITEiView protein in PROSITE
PS50021 CH, 1 hit
PS50841 DIX, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiDIXC1_HUMAN
AccessioniPrimary (citable) accession number: Q155Q3
Secondary accession number(s): A1A5D8
, E9PRV4, Q6P2J8, Q6PIK4, Q86SR7, Q8IVY4, Q96N69, Q9C0C8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 15, 2007
Last sequence update: May 15, 2007
Last modified: November 7, 2018
This is version 117 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. Human chromosome 11
    Human chromosome 11: entries, gene names and cross-references to MIM
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