UniProtKB - Q15583 (TGIF1_HUMAN)
Homeobox protein TGIF1
TGIF1
Functioni
Binds to a retinoid X receptor (RXR) responsive element from the cellular retinol-binding protein II promoter (CRBPII-RXRE). Inhibits the 9-cis-retinoic acid-dependent RXR alpha transcription activation of the retinoic acid responsive element. Active transcriptional corepressor of SMAD2. Links the nodal signaling pathway to the bifurcation of the forebrain and the establishment of ventral midline structures. May participate in the transmission of nuclear signals during development and in the adult, as illustrated by the down-modulation of the RXR alpha activities.
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
DNA bindingi | 164 – 226 | Homeobox; TALE-typePROSITE-ProRule annotationAdd BLAST | 63 |
GO - Molecular functioni
- DNA-binding transcription factor activity Source: ProtInc
- DNA-binding transcription factor activity, RNA polymerase II-specific Source: NTNU_SB
- DNA-binding transcription repressor activity, RNA polymerase II-specific Source: NTNU_SB
- RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: NTNU_SB
- sequence-specific double-stranded DNA binding Source: ARUK-UCL
GO - Biological processi
- negative regulation of transcription by RNA polymerase II Source: NTNU_SB
- regulation of transcription by RNA polymerase II Source: GO_Central
Keywordsi
Molecular function | DNA-binding, Repressor |
Biological process | Transcription, Transcription regulation |
Enzyme and pathway databases
PathwayCommonsi | Q15583 |
Reactomei | R-HSA-2173795, Downregulation of SMAD2/3:SMAD4 transcriptional activity R-HSA-2173796, SMAD2/SMAD3:SMAD4 heterotrimer regulates transcription |
SignaLinki | Q15583 |
SIGNORi | Q15583 |
Names & Taxonomyi
Protein namesi | Recommended name: Homeobox protein TGIF1Alternative name(s): 5'-TG-3'-interacting factor 1 |
Gene namesi | Name:TGIF1 Synonyms:TGIF |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:11776, TGIF1 |
MIMi | 602630, gene |
neXtProti | NX_Q15583 |
VEuPathDBi | HostDB:ENSG00000177426 |
Subcellular locationi
Nucleus
Nucleus
- nucleoplasm Source: HPA
Other locations
- chromatin Source: NTNU_SB
Keywords - Cellular componenti
NucleusPathology & Biotechi
Involvement in diseasei
Holoprosencephaly 4 (HPE4)2 Publications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_009961 | 157 | S → C in HPE4. 1 PublicationCorresponds to variant dbSNP:rs121909066Ensembl. | 1 | |
Natural variantiVAR_009962 | 192 | P → R in HPE4. 1 PublicationCorresponds to variant dbSNP:rs121909067Ensembl. | 1 | |
Natural variantiVAR_023803 | 236 | Q → L in HPE4. 1 PublicationCorresponds to variant dbSNP:rs28939693Ensembl. | 1 | |
Natural variantiVAR_009963 | 280 | T → A in HPE4. 1 PublicationCorresponds to variant dbSNP:rs121909068Ensembl. | 1 | |
Natural variantiVAR_009964 | 291 | S → F in HPE4. 1 PublicationCorresponds to variant dbSNP:rs121909069Ensembl. | 1 |
Keywords - Diseasei
Disease variant, HoloprosencephalyOrganism-specific databases
DisGeNETi | 7050 |
GeneReviewsi | TGIF1 |
MalaCardsi | TGIF1 |
MIMi | 142946, phenotype |
OpenTargetsi | ENSG00000177426 |
Orphaneti | 93925, Alobar holoprosencephaly 93924, Lobar holoprosencephaly 280200, Microform holoprosencephaly 93926, Midline interhemispheric variant of holoprosencephaly 220386, Semilobar holoprosencephaly 280195, Septopreoptic holoprosencephaly |
PharmGKBi | PA36489 |
Miscellaneous databases
Pharosi | Q15583, Tbio |
Genetic variation databases
BioMutai | TGIF1 |
DMDMi | 215274200 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000049318 | 1 – 401 | Homeobox protein TGIF1Add BLAST | 401 |
Proteomic databases
EPDi | Q15583 |
jPOSTi | Q15583 |
MassIVEi | Q15583 |
MaxQBi | Q15583 |
PaxDbi | Q15583 |
PeptideAtlasi | Q15583 |
PRIDEi | Q15583 |
ProteomicsDBi | 60646 [Q15583-1] 60647 [Q15583-2] 60648 [Q15583-3] 958 |
PTM databases
iPTMneti | Q15583 |
PhosphoSitePlusi | Q15583 |
Expressioni
Gene expression databases
Bgeei | ENSG00000177426, Expressed in left coronary artery and 222 other tissues |
ExpressionAtlasi | Q15583, baseline and differential |
Genevisiblei | Q15583, HS |
Organism-specific databases
HPAi | ENSG00000177426, Low tissue specificity |
Interactioni
Subunit structurei
Binary interactionsi
Q15583
Isoform 2 [Q15583-2]
With | #Exp. | IntAct |
---|---|---|
APP [P05067] | 3 | EBI-12691451,EBI-77613 |
CTBP1 - isoform 2 [Q13363-2] | 3 | EBI-12691451,EBI-10171858 |
CTBP2 [P56545] | 3 | EBI-12691451,EBI-741533 |
PIK3R1 - isoform 2 [P27986-2] | 3 | EBI-12691451,EBI-9090282 |
Protein-protein interaction databases
BioGRIDi | 112908, 29 interactors |
CORUMi | Q15583 |
ELMi | Q15583 |
IntActi | Q15583, 22 interactors |
MINTi | Q15583 |
STRINGi | 9606.ENSP00000327959 |
Miscellaneous databases
RNActi | Q15583, protein |
Structurei
Secondary structure
3D structure databases
AlphaFoldDBi | Q15583 |
BMRBi | Q15583 |
SASBDBi | Q15583 |
SMRi | Q15583 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q15583 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 34 | DisorderedSequence analysisAdd BLAST | 34 | |
Regioni | 78 – 127 | DisorderedSequence analysisAdd BLAST | 50 | |
Regioni | 139 – 170 | DisorderedSequence analysisAdd BLAST | 32 | |
Regioni | 274 – 293 | DisorderedSequence analysisAdd BLAST | 20 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 153 – 157 | CTBP-binding motif | 5 |
Sequence similaritiesi
Keywords - Domaini
HomeoboxPhylogenomic databases
eggNOGi | KOG0773, Eukaryota |
GeneTreei | ENSGT00940000155230 |
InParanoidi | Q15583 |
OMAi | FPLCQSV |
OrthoDBi | 1573375at2759 |
PhylomeDBi | Q15583 |
TreeFami | TF318093 |
Family and domain databases
CDDi | cd00086, homeodomain, 1 hit |
InterProi | View protein in InterPro IPR009057, Homeobox-like_sf IPR001356, Homeobox_dom IPR008422, Homeobox_KN_domain |
Pfami | View protein in Pfam PF05920, Homeobox_KN, 1 hit |
SMARTi | View protein in SMART SM00389, HOX, 1 hit |
SUPFAMi | SSF46689, SSF46689, 1 hit |
PROSITEi | View protein in PROSITE PS50071, HOMEOBOX_2, 1 hit |
s (4+)i Sequence
Sequence statusi: Complete.
This entry describes 4 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 4 described isoforms and 10 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MVLAQSRVSA GVGSPHCSGS GGGGSDSFPW PASHPGNPQC SFSTAFLASP
60 70 80 90 100
RLSRGTLAYL PPAPWSSLAT PSALLGSSCA PPPPPARCPQ PRALSPELGT
110 120 130 140 150
KAGPRRPHRW ELPRSPSQGA QGPAPRRRLL ETMKGIVAAS GSETEDEDSM
160 170 180 190 200
DIPLDLSSSA GSGKRRRRGN LPKESVQILR DWLYEHRYNA YPSEQEKALL
210 220 230 240 250
SQQTHLSTLQ VCNWFINARR RLLPDMLRKD GKDPNQFTIS RRGAKISETS
260 270 280 290 300
SVESVMGIKN FMPALEETPF HSCTAGPNPT LGRPLSPKPS SPGSVLARPS
310 320 330 340 350
VICHTTVTAL KDVPFSLCQS VGVGQNTDIQ QIAAKNFTDT SLMYPEDTCK
360 370 380 390 400
SGPSTNTQSG LFNTPPPTPP DLNQDFSGFQ LLVDVALKRA AEMELQAKLT
A
Computationally mapped potential isoform sequencesi
There are 10 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketF8VUY0 | F8VUY0_HUMAN | Homeobox protein TGIF1 | TGIF1 | 219 | Annotation score: | ||
F8VVS9 | F8VVS9_HUMAN | Homeobox protein TGIF1 | TGIF1 | 245 | Annotation score: | ||
F8VW34 | F8VW34_HUMAN | Homeobox protein TGIF1 | TGIF1 | 131 | Annotation score: | ||
F8VXG3 | F8VXG3_HUMAN | Homeobox protein TGIF1 | TGIF1 | 91 | Annotation score: | ||
F8VX54 | F8VX54_HUMAN | Homeobox protein TGIF1 | TGIF1 | 133 | Annotation score: | ||
F8VYL4 | F8VYL4_HUMAN | Homeobox protein TGIF1 | TGIF1 | 118 | Annotation score: | ||
F8VZH2 | F8VZH2_HUMAN | Homeobox protein TGIF1 | TGIF1 | 137 | Annotation score: | ||
F8VWK5 | F8VWK5_HUMAN | Homeobox protein TGIF1 | TGIF1 | 167 | Annotation score: | ||
F8W1J9 | F8W1J9_HUMAN | Homeobox protein TGIF1 | TGIF1 | 132 | Annotation score: | ||
J3KS32 | J3KS32_HUMAN | Homeobox protein TGIF1 | TGIF1 | 84 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 96 | P → Q in AAH31268 (PubMed:15489334).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_009961 | 157 | S → C in HPE4. 1 PublicationCorresponds to variant dbSNP:rs121909066Ensembl. | 1 | |
Natural variantiVAR_009962 | 192 | P → R in HPE4. 1 PublicationCorresponds to variant dbSNP:rs121909067Ensembl. | 1 | |
Natural variantiVAR_023803 | 236 | Q → L in HPE4. 1 PublicationCorresponds to variant dbSNP:rs28939693Ensembl. | 1 | |
Natural variantiVAR_009963 | 280 | T → A in HPE4. 1 PublicationCorresponds to variant dbSNP:rs121909068Ensembl. | 1 | |
Natural variantiVAR_047363 | 289 | P → S. Corresponds to variant dbSNP:rs11571512Ensembl. | 1 | |
Natural variantiVAR_009964 | 291 | S → F in HPE4. 1 PublicationCorresponds to variant dbSNP:rs121909069Ensembl. | 1 | |
Natural variantiVAR_020151 | 292 | P → L. Corresponds to variant dbSNP:rs2229333Ensembl. | 1 | |
Natural variantiVAR_061268 | 292 | P → S2 PublicationsCorresponds to variant dbSNP:rs4468717Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_046848 | 1 – 149 | Missing in isoform 4. 1 PublicationAdd BLAST | 149 | |
Alternative sequenceiVSP_013020 | 1 – 129 | Missing in isoform 2. 4 PublicationsAdd BLAST | 129 | |
Alternative sequenceiVSP_043108 | 1 – 115 | Missing in isoform 3. 1 PublicationAdd BLAST | 115 | |
Alternative sequenceiVSP_043109 | 116 – 134 | PSQGA…LETMK → MTCSGKSCALARSSLTSSQ in isoform 3. 1 PublicationAdd BLAST | 19 | |
Alternative sequenceiVSP_013021 | 130 – 133 | LETM → MKGK in isoform 2. 4 Publications | 4 |
Sequence databases
Genome annotation databases
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2LK2 | NMR | - | A | 171-248 | [»] | |
6FQP | X-ray | 2.42 | A/B | 150-248 | [»] | |
6FQQ | X-ray | 3.25 | A/B/D/E | 161-229 | [»] | |
AlphaFoldDBi | Q15583 | |||||
BMRBi | Q15583 | |||||
SASBDBi | Q15583 | |||||
SMRi | Q15583 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 112908, 29 interactors |
CORUMi | Q15583 |
ELMi | Q15583 |
IntActi | Q15583, 22 interactors |
MINTi | Q15583 |
STRINGi | 9606.ENSP00000327959 |
PTM databases
iPTMneti | Q15583 |
PhosphoSitePlusi | Q15583 |
Genetic variation databases
BioMutai | TGIF1 |
DMDMi | 215274200 |
Proteomic databases
EPDi | Q15583 |
jPOSTi | Q15583 |
MassIVEi | Q15583 |
MaxQBi | Q15583 |
PaxDbi | Q15583 |
PeptideAtlasi | Q15583 |
PRIDEi | Q15583 |
ProteomicsDBi | 60646 [Q15583-1] 60647 [Q15583-2] 60648 [Q15583-3] 958 |
Protocols and materials databases
ABCDi | Q15583, 1 sequenced antibody |
Antibodypediai | 3890, 381 antibodies from 32 providers |
DNASUi | 7050 |
Genome annotation databases
Organism-specific databases
CTDi | 7050 |
DisGeNETi | 7050 |
GeneCardsi | TGIF1 |
GeneReviewsi | TGIF1 |
HGNCi | HGNC:11776, TGIF1 |
HPAi | ENSG00000177426, Low tissue specificity |
MalaCardsi | TGIF1 |
MIMi | 142946, phenotype 602630, gene |
neXtProti | NX_Q15583 |
OpenTargetsi | ENSG00000177426 |
Orphaneti | 93925, Alobar holoprosencephaly 93924, Lobar holoprosencephaly 280200, Microform holoprosencephaly 93926, Midline interhemispheric variant of holoprosencephaly 220386, Semilobar holoprosencephaly 280195, Septopreoptic holoprosencephaly |
PharmGKBi | PA36489 |
VEuPathDBi | HostDB:ENSG00000177426 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG0773, Eukaryota |
GeneTreei | ENSGT00940000155230 |
InParanoidi | Q15583 |
OMAi | FPLCQSV |
OrthoDBi | 1573375at2759 |
PhylomeDBi | Q15583 |
TreeFami | TF318093 |
Enzyme and pathway databases
PathwayCommonsi | Q15583 |
Reactomei | R-HSA-2173795, Downregulation of SMAD2/3:SMAD4 transcriptional activity R-HSA-2173796, SMAD2/SMAD3:SMAD4 heterotrimer regulates transcription |
SignaLinki | Q15583 |
SIGNORi | Q15583 |
Miscellaneous databases
BioGRID-ORCSi | 7050, 71 hits in 1101 CRISPR screens |
ChiTaRSi | TGIF1, human |
EvolutionaryTracei | Q15583 |
GeneWikii | Homeobox_protein_TGIF1 |
GenomeRNAii | 7050 |
Pharosi | Q15583, Tbio |
PROi | PR:Q15583 |
RNActi | Q15583, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000177426, Expressed in left coronary artery and 222 other tissues |
ExpressionAtlasi | Q15583, baseline and differential |
Genevisiblei | Q15583, HS |
Family and domain databases
CDDi | cd00086, homeodomain, 1 hit |
InterProi | View protein in InterPro IPR009057, Homeobox-like_sf IPR001356, Homeobox_dom IPR008422, Homeobox_KN_domain |
Pfami | View protein in Pfam PF05920, Homeobox_KN, 1 hit |
SMARTi | View protein in SMART SM00389, HOX, 1 hit |
SUPFAMi | SSF46689, SSF46689, 1 hit |
PROSITEi | View protein in PROSITE PS50071, HOMEOBOX_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | TGIF1_HUMAN | |
Accessioni | Q15583Primary (citable) accession number: Q15583 Secondary accession number(s): A6NE42 Q9NRS0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | November 25, 2008 | |
Last modified: | May 25, 2022 | |
This is version 218 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 18
Human chromosome 18: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families