UniProtKB - Q15561 (TEAD4_HUMAN)
Protein
Transcriptional enhancer factor TEF-3
Gene
TEAD4
Organism
Homo sapiens (Human)
Status
Functioni
Transcription factor which plays a key role in the Hippo signaling pathway, a pathway involved in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Acts by mediating gene expression of YAP1 and WWTR1/TAZ, thereby regulating cell proliferation, migration and epithelial mesenchymal transition (EMT) induction. Binds specifically and non-cooperatively to the Sph and GT-IIC 'enhansons' (5'-GTGGAATGT-3') and activates transcription. Binds to the M-CAT motif.2 Publications
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
DNA bindingi | 36 – 112 | TEAPROSITE-ProRule annotationAdd BLAST | 77 |
GO - Molecular functioni
- DNA-binding transcription activator activity, RNA polymerase II-specific Source: GO_Central
- DNA-binding transcription factor activity Source: UniProtKB
- DNA-binding transcription factor activity, RNA polymerase II-specific Source: NTNU_SB
- RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: GO_Central
GO - Biological processi
- embryonic organ development Source: GO_Central
- hippo signaling Source: UniProtKB
- muscle organ development Source: ProtInc
- positive regulation of transcription by RNA polymerase II Source: GO_Central
- regulation of transcription by RNA polymerase II Source: ProtInc
- skeletal system development Source: ProtInc
- transcription initiation from RNA polymerase II promoter Source: Reactome
Keywordsi
Molecular function | Activator, DNA-binding |
Biological process | Transcription, Transcription regulation |
Enzyme and pathway databases
PathwayCommonsi | Q15561 |
Reactomei | R-HSA-2032785, YAP1- and WWTR1 (TAZ)-stimulated gene expression R-HSA-8951671, RUNX3 regulates YAP1-mediated transcription |
SIGNORi | Q15561 |
Names & Taxonomyi
Protein namesi | Recommended name: Transcriptional enhancer factor TEF-3Alternative name(s): TEA domain family member 4 Short name: TEAD-4 Transcription factor 13-like 1 Transcription factor RTEF-1 |
Gene namesi | Name:TEAD4 Synonyms:RTEF1, TCF13L1, TEF3 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:11717, TEAD4 |
MIMi | 601714, gene |
neXtProti | NX_Q15561 |
VEuPathDBi | HostDB:ENSG00000197905.8 |
Subcellular locationi
Nucleus
Nucleus
- nuclear chromatin Source: NTNU_SB
- nucleoplasm Source: HPA
- nucleus Source: CAFA
Other locations
- transcription regulator complex Source: GO_Central
Keywords - Cellular componenti
NucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 266 | D → A: Reduced transforming ability. 1 Publication | 1 | |
Mutagenesisi | 297 | K → A: Important loss of interaction with YAP1 and complete loss of transforming ability. 1 Publication | 1 | |
Mutagenesisi | 299 | W → A: Important loss of interaction with YAP1 and complete loss of transforming ability. 1 Publication | 1 | |
Mutagenesisi | 337 | F → A: Reduced interaction with YAP1. 1 Publication | 1 | |
Mutagenesisi | 373 | F → A: Reduced transforming ability. 1 Publication | 1 | |
Mutagenesisi | 380 | L → A: Reduced transforming ability. 1 Publication | 1 | |
Mutagenesisi | 391 | E → A: Reduced transforming ability. 1 Publication | 1 | |
Mutagenesisi | 393 | F → A: Reduced transforming ability. 1 Publication | 1 | |
Mutagenesisi | 427 | H → A: Reduced transforming ability. 1 Publication | 1 | |
Mutagenesisi | 429 | Y → A or H: Loss of interaction with YAP1 and also activation by YAP1. 2 Publications | 1 | |
Mutagenesisi | 429 | Y → A: Important loss of interaction with YAP1 and complete loss of transforming ability. 2 Publications | 1 |
Organism-specific databases
DisGeNETi | 7004 |
OpenTargetsi | ENSG00000197905 |
PharmGKBi | PA36435 |
Miscellaneous databases
Pharosi | Q15561, Tbio |
Chemistry databases
ChEMBLi | CHEMBL4295828 |
Genetic variation databases
BioMutai | TEAD4 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000205937 | 1 – 434 | Transcriptional enhancer factor TEF-3Add BLAST | 434 |
Proteomic databases
EPDi | Q15561 |
jPOSTi | Q15561 |
MassIVEi | Q15561 |
MaxQBi | Q15561 |
PaxDbi | Q15561 |
PeptideAtlasi | Q15561 |
PRIDEi | Q15561 |
ProteomicsDBi | 34410 60634 [Q15561-1] 60635 [Q15561-2] |
PTM databases
iPTMneti | Q15561 |
PhosphoSitePlusi | Q15561 |
SwissPalmi | Q15561 |
Expressioni
Tissue specificityi
Preferentially expressed in skeletal muscle. Lower levels in pancreas, placenta, and heart.
Gene expression databases
Bgeei | ENSG00000197905, Expressed in gastrocnemius and 187 other tissues |
ExpressionAtlasi | Q15561, baseline and differential |
Genevisiblei | Q15561, HS |
Organism-specific databases
HPAi | ENSG00000197905, Tissue enhanced (skeletal) |
Interactioni
Subunit structurei
Interacts with YAP1 and WWTR1/TAZ.
3 PublicationsBinary interactionsi
Hide detailsQ15561
Protein-protein interaction databases
BioGRIDi | 112863, 65 interactors |
DIPi | DIP-61626N |
IntActi | Q15561, 48 interactors |
MINTi | Q15561 |
STRINGi | 9606.ENSP00000352926 |
Chemistry databases
BindingDBi | Q15561 |
Miscellaneous databases
RNActi | Q15561, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q15561 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Phylogenomic databases
eggNOGi | KOG3841, Eukaryota |
GeneTreei | ENSGT00950000182956 |
HOGENOMi | CLU_012515_0_0_1 |
InParanoidi | Q15561 |
OrthoDBi | 823827at2759 |
PhylomeDBi | Q15561 |
Family and domain databases
Gene3Di | 1.10.10.1610, 1 hit |
InterProi | View protein in InterPro IPR000818, TEA/ATTS_dom IPR038096, TEA/ATTS_sf IPR027255, TEF-3 IPR016361, TEF_metazoa IPR041086, YBD |
Pfami | View protein in Pfam PF01285, TEA, 1 hit PF17725, YBD, 1 hit |
PIRSFi | PIRSF002603, TEF, 1 hit PIRSF500722, TEF-3, 1 hit |
PRINTSi | PR00065, TEADOMAIN |
SMARTi | View protein in SMART SM00426, TEA, 1 hit |
PROSITEi | View protein in PROSITE PS00554, TEA_1, 1 hit PS51088, TEA_2, 1 hit |
s (3+)i Sequence
Sequence statusi: Complete.
This entry describes 3 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 3 described isoforms and 7 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: Q15561-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MEGTAGTITS NEWSSPTSPE GSTASGGSQA LDKPIDNDAE GVWSPDIEQS
60 70 80 90 100
FQEALAIYPP CGRRKIILSD EGKMYGRNEL IARYIKLRTG KTRTRKQVSS
110 120 130 140 150
HIQVLARRKA REIQAKLKDQ AAKDKALQSM AAMSSAQIIS ATAFHSSMAL
160 170 180 190 200
ARGPGRPAVS GFWQGALPGQ AGTSHDVKPF SQQTYAVQPP LPLPGFESPA
210 220 230 240 250
GPAPSPSAPP APPWQGRSVA SSKLWMLEFS AFLEQQQDPD TYNKHLFVHI
260 270 280 290 300
GQSSPSYSDP YLEAVDIRQI YDKFPEKKGG LKDLFERGPS NAFFLVKFWA
310 320 330 340 350
DLNTNIEDEG SSFYGVSSQY ESPENMIITC STKVCSFGKQ VVEKVETEYA
360 370 380 390 400
RYENGHYSYR IHRSPLCEYM INFIHKLKHL PEKYMMNSVL ENFTILQVVT
410 420 430
NRDTQETLLC IAYVFEVSAS EHGAQHHIYR LVKE
Computationally mapped potential isoform sequencesi
There are 7 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketQ53GI4 | Q53GI4_HUMAN | TEA domain family member 4 isoform ... | TEAD4 | 434 | Annotation score: | ||
A0A0A0MRF3 | A0A0A0MRF3_HUMAN | Transcriptional enhancer factor TEF... | TEAD4 | 391 | Annotation score: | ||
H0YGS2 | H0YGS2_HUMAN | Transcriptional enhancer factor TEF... | TEAD4 | 175 | Annotation score: | ||
H0Y7B0 | H0Y7B0_HUMAN | Transcriptional enhancer factor TEF... | TEAD4 | 110 | Annotation score: | ||
H0YFF9 | H0YFF9_HUMAN | Transcriptional enhancer factor TEF... | TEAD4 | 72 | Annotation score: | ||
H0YG62 | H0YG62_HUMAN | Transcriptional enhancer factor TEF... | TEAD4 | 189 | Annotation score: | ||
H0YFK0 | H0YFK0_HUMAN | Transcriptional enhancer factor TEF... | TEAD4 | 22 | Annotation score: |
Sequence cautioni
The sequence CAA64212 differs from that shown. Unusual initiator. The initiator methionine is coded by a non-canonical ATA isoleucine codon.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 39 | A → G in AAC50763 (PubMed:8921372).Curated | 1 | |
Sequence conflicti | 149 | A → R in AAC50763 (PubMed:8921372).Curated | 1 | |
Sequence conflicti | 172 | G → E in AAC50763 (PubMed:8921372).Curated | 1 | |
Sequence conflicti | 218 – 220 | SVA → RRG in AAC50763 (PubMed:8921372).Curated | 3 | |
Sequence conflicti | 258 – 259 | SD → LR in AAC50763 (PubMed:8921372).Curated | 2 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_052279 | 323 | P → S. Corresponds to variant dbSNP:rs11550887Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_043099 | 1 – 129 | Missing in isoform 2. 1 PublicationAdd BLAST | 129 | |
Alternative sequenceiVSP_045657 | 119 – 161 | Missing in isoform 3. 1 PublicationAdd BLAST | 43 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U63824 mRNA Translation: AAC50763.1 BX640921 mRNA Translation: CAE45959.1 AC005911 Genomic DNA No translation available. AC125807 Genomic DNA No translation available. AC131234 Genomic DNA No translation available. CH471116 Genomic DNA Translation: EAW88872.1 BC015497 mRNA Translation: AAH15497.2 X94438 mRNA Translation: CAA64212.2 Sequence problems. AY101179 mRNA Translation: AAM89497.1 |
CCDSi | CCDS31729.1 [Q15561-1] CCDS31730.1 [Q15561-3] CCDS41737.1 [Q15561-2] |
RefSeqi | NP_003204.2, NM_003213.3 [Q15561-1] NP_958849.1, NM_201441.2 [Q15561-3] NP_958851.1, NM_201443.2 [Q15561-2] |
Genome annotation databases
Ensembli | ENST00000397122; ENSP00000380311; ENSG00000197905 [Q15561-2] |
GeneIDi | 7004 |
KEGGi | hsa:7004 |
UCSCi | uc001qln.4, human [Q15561-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U63824 mRNA Translation: AAC50763.1 BX640921 mRNA Translation: CAE45959.1 AC005911 Genomic DNA No translation available. AC125807 Genomic DNA No translation available. AC131234 Genomic DNA No translation available. CH471116 Genomic DNA Translation: EAW88872.1 BC015497 mRNA Translation: AAH15497.2 X94438 mRNA Translation: CAA64212.2 Sequence problems. AY101179 mRNA Translation: AAM89497.1 |
CCDSi | CCDS31729.1 [Q15561-1] CCDS31730.1 [Q15561-3] CCDS41737.1 [Q15561-2] |
RefSeqi | NP_003204.2, NM_003213.3 [Q15561-1] NP_958849.1, NM_201441.2 [Q15561-3] NP_958851.1, NM_201443.2 [Q15561-2] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
5GZB | X-ray | 2.70 | A | 36-139 | [»] | |
5NO6 | X-ray | 2.88 | I/N | 40-112 | [»] | |
5OAQ | X-ray | 1.95 | A | 217-434 | [»] | |
6GE3 | X-ray | 1.85 | A | 216-434 | [»] | |
6GE4 | X-ray | 1.97 | A | 216-434 | [»] | |
6GE5 | X-ray | 2.05 | A | 216-434 | [»] | |
6GE6 | X-ray | 1.80 | A | 216-434 | [»] | |
6GEC | X-ray | 1.70 | A | 216-434 | [»] | |
6GEE | X-ray | 1.96 | A | 216-434 | [»] | |
6GEG | X-ray | 2.23 | A | 216-434 | [»] | |
6GEI | X-ray | 1.65 | A | 216-434 | [»] | |
6GEK | X-ray | 2.28 | A/B | 216-434 | [»] | |
6HIK | X-ray | 1.65 | A | 216-434 | [»] | |
6Q2X | X-ray | 2.10 | A | 217-434 | [»] | |
6Q36 | X-ray | 2.01 | A/B | 217-434 | [»] | |
6SEN | X-ray | 1.65 | A/B | 217-434 | [»] | |
6SEO | X-ray | 2.55 | A | 217-434 | [»] | |
SMRi | Q15561 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 112863, 65 interactors |
DIPi | DIP-61626N |
IntActi | Q15561, 48 interactors |
MINTi | Q15561 |
STRINGi | 9606.ENSP00000352926 |
Chemistry databases
BindingDBi | Q15561 |
ChEMBLi | CHEMBL4295828 |
PTM databases
iPTMneti | Q15561 |
PhosphoSitePlusi | Q15561 |
SwissPalmi | Q15561 |
Genetic variation databases
BioMutai | TEAD4 |
Proteomic databases
EPDi | Q15561 |
jPOSTi | Q15561 |
MassIVEi | Q15561 |
MaxQBi | Q15561 |
PaxDbi | Q15561 |
PeptideAtlasi | Q15561 |
PRIDEi | Q15561 |
ProteomicsDBi | 34410 60634 [Q15561-1] 60635 [Q15561-2] |
Protocols and materials databases
Antibodypediai | 22199, 273 antibodies |
DNASUi | 7004 |
Genome annotation databases
Ensembli | ENST00000397122; ENSP00000380311; ENSG00000197905 [Q15561-2] |
GeneIDi | 7004 |
KEGGi | hsa:7004 |
UCSCi | uc001qln.4, human [Q15561-1] |
Organism-specific databases
CTDi | 7004 |
DisGeNETi | 7004 |
GeneCardsi | TEAD4 |
HGNCi | HGNC:11717, TEAD4 |
HPAi | ENSG00000197905, Tissue enhanced (skeletal) |
MIMi | 601714, gene |
neXtProti | NX_Q15561 |
OpenTargetsi | ENSG00000197905 |
PharmGKBi | PA36435 |
VEuPathDBi | HostDB:ENSG00000197905.8 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG3841, Eukaryota |
GeneTreei | ENSGT00950000182956 |
HOGENOMi | CLU_012515_0_0_1 |
InParanoidi | Q15561 |
OrthoDBi | 823827at2759 |
PhylomeDBi | Q15561 |
Enzyme and pathway databases
PathwayCommonsi | Q15561 |
Reactomei | R-HSA-2032785, YAP1- and WWTR1 (TAZ)-stimulated gene expression R-HSA-8951671, RUNX3 regulates YAP1-mediated transcription |
SIGNORi | Q15561 |
Miscellaneous databases
BioGRID-ORCSi | 7004, 44 hits in 897 CRISPR screens |
ChiTaRSi | TEAD4, human |
GeneWikii | TEAD4 |
GenomeRNAii | 7004 |
Pharosi | Q15561, Tbio |
PROi | PR:Q15561 |
RNActi | Q15561, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000197905, Expressed in gastrocnemius and 187 other tissues |
ExpressionAtlasi | Q15561, baseline and differential |
Genevisiblei | Q15561, HS |
Family and domain databases
Gene3Di | 1.10.10.1610, 1 hit |
InterProi | View protein in InterPro IPR000818, TEA/ATTS_dom IPR038096, TEA/ATTS_sf IPR027255, TEF-3 IPR016361, TEF_metazoa IPR041086, YBD |
Pfami | View protein in Pfam PF01285, TEA, 1 hit PF17725, YBD, 1 hit |
PIRSFi | PIRSF002603, TEF, 1 hit PIRSF500722, TEF-3, 1 hit |
PRINTSi | PR00065, TEADOMAIN |
SMARTi | View protein in SMART SM00426, TEA, 1 hit |
PROSITEi | View protein in PROSITE PS00554, TEA_1, 1 hit PS51088, TEA_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | TEAD4_HUMAN | |
Accessioni | Q15561Primary (citable) accession number: Q15561 Secondary accession number(s): H0Y308 Q96BK2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | November 25, 2008 | |
Last modified: | February 10, 2021 | |
This is version 184 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 12
Human chromosome 12: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references