UniProtKB - Q15528 (MED22_HUMAN)
Mediator of RNA polymerase II transcription subunit 22
MED22
Functioni
GO - Molecular functioni
- transcription coregulator activity Source: InterPro
GO - Biological processi
- regulation of transcription by RNA polymerase II Source: InterPro
Keywordsi
Molecular function | Activator |
Biological process | Transcription, Transcription regulation |
Enzyme and pathway databases
PathwayCommonsi | Q15528 |
Reactomei | R-HSA-1989781, PPARA activates gene expression R-HSA-381340, Transcriptional regulation of white adipocyte differentiation |
Names & Taxonomyi
Protein namesi | Recommended name: Mediator of RNA polymerase II transcription subunit 22Alternative name(s): Mediator complex subunit 22 Surfeit locus protein 5 Short name: Surf-5 |
Gene namesi | Name:MED22 Synonyms:SURF5 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:11477, MED22 |
MIMi | 185641, gene |
neXtProti | NX_Q15528 |
VEuPathDBi | HostDB:ENSG00000148297.15 |
Subcellular locationi
Nucleus
- Nucleus Curated
Nucleus
- mediator complex Source: MGI
Other locations
- cytoplasm Source: ProtInc
Keywords - Cellular componenti
NucleusPathology & Biotechi
Organism-specific databases
DisGeNETi | 6837 |
OpenTargetsi | ENSG00000148297 |
PharmGKBi | PA162395486 |
Miscellaneous databases
Pharosi | Q15528, Tbio |
Genetic variation databases
BioMutai | MED22 |
DMDMi | 6175074 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000178838 | 1 – 200 | Mediator of RNA polymerase II transcription subunit 22Add BLAST | 200 |
Proteomic databases
EPDi | Q15528 |
jPOSTi | Q15528 |
MassIVEi | Q15528 |
MaxQBi | Q15528 |
PaxDbi | Q15528 |
PeptideAtlasi | Q15528 |
PRIDEi | Q15528 |
ProteomicsDBi | 60619 [Q15528-1] 60620 [Q15528-2] |
TopDownProteomicsi | Q15528-2 [Q15528-2] |
PTM databases
iPTMneti | Q15528 |
PhosphoSitePlusi | Q15528 |
Expressioni
Gene expression databases
Bgeei | ENSG00000148297, Expressed in heart left ventricle and 195 other tissues |
ExpressionAtlasi | Q15528, baseline and differential |
Genevisiblei | Q15528, HS |
Organism-specific databases
HPAi | ENSG00000148297, Low tissue specificity |
Interactioni
Subunit structurei
Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP.
1 PublicationBinary interactionsi
Hide detailsQ15528
Isoform Surf5A [Q15528-2]
Protein-protein interaction databases
BioGRIDi | 112704, 60 interactors |
ComplexPortali | CPX-3227, Core mediator complex |
CORUMi | Q15528 |
IntActi | Q15528, 36 interactors |
STRINGi | 9606.ENSP00000482438 |
Miscellaneous databases
RNActi | Q15528, protein |
Family & Domainsi
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 93 – 122 | Sequence analysisAdd BLAST | 30 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 136 – 140 | Poly-Ser | 5 |
Sequence similaritiesi
Keywords - Domaini
Coiled coilPhylogenomic databases
eggNOGi | KOG3304, Eukaryota |
GeneTreei | ENSGT00390000004339 |
HOGENOMi | CLU_117242_0_0_1 |
InParanoidi | Q15528 |
OMAi | TQCEQDT |
PhylomeDBi | Q15528 |
TreeFami | TF323390 |
Family and domain databases
Gene3Di | 1.20.58.1600, 1 hit |
InterProi | View protein in InterPro IPR009332, Med22 IPR038427, MED22_sf |
PANTHERi | PTHR12434, PTHR12434, 1 hit |
Pfami | View protein in Pfam PF06179, Med22, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 4 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MAQQRALPQS KETLLQSYNK RLKDDIKSIM DNFTEIIKTA KIEDETQVSR
60 70 80 90 100
ATQGEQDNYE MHVRAANIVR AGESLMKLVS DLKQFLILND FPSVNEAIDQ
110 120 130 140 150
RNQQLRTLQE ECDRKLITLR DEISIDLYEL EEEYYSSSSS LCEANDLPLC
160 170 180 190 200
EAYGRLDLDT DSADGLSAPL LASPEPSAGP LQVAAPAHSH AGGPGPTEHA
The sequence of this isoform differs from the canonical sequence as follows:
138-200: SSSLCEANDLPLCEAYGRLDLDTDSADGLSAPLLASPEPSAGPLQVAAPAHSHAGGPGPTEHA → RYK
Computationally mapped potential isoform sequencesi
There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketE9PGW7 | E9PGW7_HUMAN | Mediator complex subunit 22 | MED22 | 111 | Annotation score: | ||
E7EN96 | E7EN96_HUMAN | Mediator complex subunit 22 | MED22 | 135 | Annotation score: | ||
Q5T8T7 | Q5T8T7_HUMAN | Mediator complex subunit 22 | MED22 RP11-244N20.9-003 | 134 | Annotation score: | ||
Q5T8T8 | Q5T8T8_HUMAN | Mediator complex subunit 22 | MED22 RP11-244N20.9-005 | 137 | Annotation score: |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_006309 | 138 – 200 | SSSLC…PTEHA → RYK in isoform Surf5A. 3 PublicationsAdd BLAST | 63 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X85178 mRNA Translation: CAA59462.1 AJ224639 Genomic DNA Translation: CAA12052.1 AJ224639 Genomic DNA Translation: CAA12053.1 AJ224358 mRNA Translation: CAA11915.1 AJ224359 mRNA Translation: CAA11916.1 AK124518 mRNA Translation: BAG54045.1 AK126069 mRNA Translation: BAG54286.1 AL158826 Genomic DNA Translation: CAI12827.1 CH471090 Genomic DNA Translation: EAW88056.1 CH471090 Genomic DNA Translation: EAW88060.1 BC024225 mRNA Translation: AAH24225.1 BC040111 mRNA Translation: AAH40111.1 |
CCDSi | CCDS6963.1 [Q15528-1] CCDS6964.1 [Q15528-2] |
RefSeqi | NP_598395.1, NM_133640.4 [Q15528-1] NP_852468.1, NM_181491.2 [Q15528-2] |
Genome annotation databases
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X85178 mRNA Translation: CAA59462.1 AJ224639 Genomic DNA Translation: CAA12052.1 AJ224639 Genomic DNA Translation: CAA12053.1 AJ224358 mRNA Translation: CAA11915.1 AJ224359 mRNA Translation: CAA11916.1 AK124518 mRNA Translation: BAG54045.1 AK126069 mRNA Translation: BAG54286.1 AL158826 Genomic DNA Translation: CAI12827.1 CH471090 Genomic DNA Translation: EAW88056.1 CH471090 Genomic DNA Translation: EAW88060.1 BC024225 mRNA Translation: AAH24225.1 BC040111 mRNA Translation: AAH40111.1 |
CCDSi | CCDS6963.1 [Q15528-1] CCDS6964.1 [Q15528-2] |
RefSeqi | NP_598395.1, NM_133640.4 [Q15528-1] NP_852468.1, NM_181491.2 [Q15528-2] |
3D structure databases
SMRi | Q15528 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 112704, 60 interactors |
ComplexPortali | CPX-3227, Core mediator complex |
CORUMi | Q15528 |
IntActi | Q15528, 36 interactors |
STRINGi | 9606.ENSP00000482438 |
PTM databases
iPTMneti | Q15528 |
PhosphoSitePlusi | Q15528 |
Genetic variation databases
BioMutai | MED22 |
DMDMi | 6175074 |
Proteomic databases
EPDi | Q15528 |
jPOSTi | Q15528 |
MassIVEi | Q15528 |
MaxQBi | Q15528 |
PaxDbi | Q15528 |
PeptideAtlasi | Q15528 |
PRIDEi | Q15528 |
ProteomicsDBi | 60619 [Q15528-1] 60620 [Q15528-2] |
TopDownProteomicsi | Q15528-2 [Q15528-2] |
Protocols and materials databases
Antibodypediai | 18330, 171 antibodies |
DNASUi | 6837 |
Genome annotation databases
Organism-specific databases
CTDi | 6837 |
DisGeNETi | 6837 |
GeneCardsi | MED22 |
HGNCi | HGNC:11477, MED22 |
HPAi | ENSG00000148297, Low tissue specificity |
MIMi | 185641, gene |
neXtProti | NX_Q15528 |
OpenTargetsi | ENSG00000148297 |
PharmGKBi | PA162395486 |
VEuPathDBi | HostDB:ENSG00000148297.15 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG3304, Eukaryota |
GeneTreei | ENSGT00390000004339 |
HOGENOMi | CLU_117242_0_0_1 |
InParanoidi | Q15528 |
OMAi | TQCEQDT |
PhylomeDBi | Q15528 |
TreeFami | TF323390 |
Enzyme and pathway databases
PathwayCommonsi | Q15528 |
Reactomei | R-HSA-1989781, PPARA activates gene expression R-HSA-381340, Transcriptional regulation of white adipocyte differentiation |
Miscellaneous databases
BioGRID-ORCSi | 6837, 695 hits in 1008 CRISPR screens |
ChiTaRSi | MED22, human |
GeneWikii | MED22 |
GenomeRNAii | 6837 |
Pharosi | Q15528, Tbio |
PROi | PR:Q15528 |
RNActi | Q15528, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000148297, Expressed in heart left ventricle and 195 other tissues |
ExpressionAtlasi | Q15528, baseline and differential |
Genevisiblei | Q15528, HS |
Family and domain databases
Gene3Di | 1.20.58.1600, 1 hit |
InterProi | View protein in InterPro IPR009332, Med22 IPR038427, MED22_sf |
PANTHERi | PTHR12434, PTHR12434, 1 hit |
Pfami | View protein in Pfam PF06179, Med22, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | MED22_HUMAN | |
Accessioni | Q15528Primary (citable) accession number: Q15528 Secondary accession number(s): B3KW83 Q5T8U0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | July 15, 1999 | |
Last modified: | April 7, 2021 | |
This is version 168 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human chromosome 9
Human chromosome 9: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families