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Protein

Regucalcin

Gene

RGN

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Gluconolactonase with low activity towards other sugar lactones, including gulonolactone and galactonolactone. Can also hydrolyze diisopropyl phosphorofluoridate and phenylacetate (in vitro). Calcium-binding protein. Modulates Ca2+ signaling, and Ca2+-dependent cellular processes and enzyme activities (By similarity).By similarity

Caution

Gluconolactonase catalyzes a key step in ascorbic acid (vitamin C) biosynthesis, but primates lack the last enzyme in the pathway and are unable to synthesize vitamin C.Curated

Catalytic activityi

D-glucono-1,5-lactone + H2O = D-gluconate.2 Publications

Cofactori

Zn2+2 Publications, Mn2+2 Publications, Ca2+2 Publications, Mg2+2 PublicationsNote: Binds 1 divalent metal cation per subunit. Most active with Zn2+ and Mn2+ ions. The physiological cofactor is most likely Ca2+ or Mg2+.2 Publications

Kineticsi

  1. KM=2.7 mM for gluconolactone (with Zn2+ as cofactor)1 Publication
  2. KM=0.6 mM for gluconolactone (with Mn2+ as cofactor)1 Publication
  3. KM=1.3 mM for gluconolactone (with Mg2+ as cofactor)1 Publication
  4. KM=3.7 mM for gluconolactone (with Ca2+ as cofactor)1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Metal bindingi18Divalent metal cation1 Publication1
    Binding sitei101Substrate1
    Binding sitei103Substrate1
    Binding sitei121SubstrateBy similarity1
    Metal bindingi154Divalent metal cation1 Publication1
    Active sitei204Proton donor/acceptor1 Publication1
    Metal bindingi204Divalent metal cation1 Publication1

    GO - Molecular functioni

    • calcium ion binding Source: UniProtKB
    • enzyme regulator activity Source: InterPro
    • gluconolactonase activity Source: UniProtKB
    • zinc ion binding Source: UniProtKB

    GO - Biological processi

    Keywordsi

    Molecular functionHydrolase
    LigandCalcium, Metal-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Regucalcin
    Short name:
    RC
    Alternative name(s):
    Gluconolactonase (EC:3.1.1.17)
    Short name:
    GNL
    Senescence marker protein 30
    Short name:
    SMP-30
    Gene namesi
    Name:RGN
    Synonyms:SMP30
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    Proteomesi
    • UP000005640 Componenti: Chromosome X

    Organism-specific databases

    EuPathDBiHostDB:ENSG00000130988.12
    HGNCiHGNC:9989 RGN
    MIMi300212 gene
    neXtProtiNX_Q15493

    Subcellular locationi

    Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Mutagenesisi18E → A: Reduces enzyme activity by about 90%. 1 Publication1
    Mutagenesisi103N → A: Reduces enzyme activity by about 95%. 1 Publication1
    Mutagenesisi154N → A: Reduces enzyme activity by about 95%. 1 Publication1
    Mutagenesisi204D → A: Reduces enzyme activity by over 98%. 1 Publication1

    Organism-specific databases

    DisGeNETi9104
    OpenTargetsiENSG00000130988
    PharmGKBiPA34359

    Polymorphism and mutation databases

    BioMutaiRGN
    DMDMi3334328

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00001730461 – 299RegucalcinAdd BLAST299

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Modified residuei144N6-succinyllysineBy similarity1
    Modified residuei244N6-succinyllysineBy similarity1
    Modified residuei253N6-succinyllysineBy similarity1

    Proteomic databases

    PaxDbiQ15493
    PeptideAtlasiQ15493
    PRIDEiQ15493
    ProteomicsDBi60611
    60612 [Q15493-2]

    PTM databases

    iPTMnetiQ15493
    PhosphoSitePlusiQ15493

    Expressioni

    Gene expression databases

    BgeeiENSG00000130988
    CleanExiHS_RGN
    ExpressionAtlasiQ15493 baseline and differential
    GenevisibleiQ15493 HS

    Organism-specific databases

    HPAiHPA029102
    HPA029103

    Interactioni

    Subunit structurei

    Monomer.1 Publication

    Protein-protein interaction databases

    BioGridi114557, 7 interactors
    STRINGi9606.ENSP00000253303

    Structurei

    Secondary structure

    1299
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details
    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Beta strandi5 – 10Combined sources6
    Beta strandi15 – 23Combined sources9
    Turni24 – 27Combined sources4
    Beta strandi28 – 33Combined sources6
    Turni34 – 37Combined sources4
    Beta strandi38 – 43Combined sources6
    Turni44 – 46Combined sources3
    Beta strandi49 – 53Combined sources5
    Beta strandi58 – 64Combined sources7
    Beta strandi67 – 73Combined sources7
    Beta strandi76 – 81Combined sources6
    Turni82 – 85Combined sources4
    Beta strandi86 – 92Combined sources7
    Beta strandi98 – 107Combined sources10
    Beta strandi113 – 119Combined sources7
    Beta strandi132 – 137Combined sources6
    Beta strandi143 – 158Combined sources16
    Beta strandi164 – 169Combined sources6
    Helixi170 – 172Combined sources3
    Beta strandi174 – 180Combined sources7
    Turni182 – 184Combined sources3
    Beta strandi187 – 195Combined sources9
    Helixi198 – 200Combined sources3
    Beta strandi202 – 209Combined sources8
    Beta strandi214 – 219Combined sources6
    Turni220 – 222Combined sources3
    Beta strandi223 – 227Combined sources5
    Turni229 – 231Combined sources3
    Beta strandi234 – 239Combined sources6
    Beta strandi241 – 243Combined sources3
    Beta strandi245 – 252Combined sources8
    Helixi253 – 255Combined sources3
    Beta strandi257 – 263Combined sources7
    Helixi269 – 274Combined sources6
    Turni276 – 279Combined sources4
    Beta strandi281 – 285Combined sources5

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    3G4EX-ray1.42A/B3-299[»]
    3G4HX-ray1.92A/B3-299[»]
    4GNBX-ray1.50A/B1-299[»]
    4GNCX-ray1.75A/B1-299[»]
    ProteinModelPortaliQ15493
    SMRiQ15493
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ15493

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the SMP-30/CGR1 family.Curated

    Phylogenomic databases

    eggNOGiKOG4499 Eukaryota
    COG3386 LUCA
    GeneTreeiENSGT00390000014995
    HOGENOMiHOG000220627
    HOVERGENiHBG004347
    InParanoidiQ15493
    KOiK01053
    OMAiCRWDSLT
    OrthoDBiEOG091G131G
    PhylomeDBiQ15493
    TreeFamiTF323663

    Family and domain databases

    Gene3Di2.120.10.30, 1 hit
    InterProiView protein in InterPro
    IPR011042 6-blade_b-propeller_TolB-like
    IPR008367 Regucalcin
    IPR013658 SGL
    IPR005511 SMP-30
    PfamiView protein in Pfam
    PF08450 SGL, 1 hit
    PRINTSiPR01791 REGUCALCIN
    PR01790 SMP30FAMILY

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

    Isoform 1 (identifier: Q15493-1) [UniParc]FASTAAdd to basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

            10         20         30         40         50
    MSSIKIECVL PENCRCGESP VWEEVSNSLL FVDIPAKKVC RWDSFTKQVQ
    60 70 80 90 100
    RVTMDAPVSS VALRQSGGYV ATIGTKFCAL NWKEQSAVVL ATVDNDKKNN
    110 120 130 140 150
    RFNDGKVDPA GRYFAGTMAE ETAPAVLERH QGALYSLFPD HHVKKYFDQV
    160 170 180 190 200
    DISNGLDWSL DHKIFYYIDS LSYSVDAFDY DLQTGQISNR RSVYKLEKEE
    210 220 230 240 250
    QIPDGMCIDA EGKLWVACYN GGRVIRLDPV TGKRLQTVKL PVDKTTSCCF
    260 270 280 290
    GGKNYSEMYV TCARDGMDPE GLLRQPEAGG IFKITGLGVK GIAPYSYAG
    Length:299
    Mass (Da):33,253
    Last modified:November 1, 1996 - v1
    Checksum:i95BA1C73B7B77635
    GO
    Isoform 2 (identifier: Q15493-2) [UniParc]FASTAAdd to basket

    The sequence of this isoform differs from the canonical sequence as follows:
         116-187: Missing.
         188-188: S → A

    Note: No experimental confirmation available.
    Show »
    Length:227
    Mass (Da):24,968
    Checksum:i19BC938A52DA72D4
    GO

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti292 – 293IA → TS in BAD97080 (Ref. 4) Curated2

    Alternative sequence

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Alternative sequenceiVSP_025456116 – 187Missing in isoform 2. 1 PublicationAdd BLAST72
    Alternative sequenceiVSP_025457188S → A in isoform 2. 1 Publication1

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    D31815 mRNA Translation: BAA06602.1
    AB028125 mRNA Translation: BAA78693.1
    AB032064 mRNA Translation: BAA84082.1
    AK290136 mRNA Translation: BAF82825.1
    AK223360 mRNA Translation: BAD97080.1
    AL513366 Genomic DNA No translation available.
    BC050371 mRNA Translation: AAH50371.1
    BC073173 mRNA Translation: AAH73173.1
    CCDSiCCDS14272.1 [Q15493-1]
    CCDS75968.1 [Q15493-2]
    PIRiS60035
    RefSeqiNP_001269777.1, NM_001282848.1
    NP_001269778.1, NM_001282849.1 [Q15493-2]
    NP_004674.1, NM_004683.5 [Q15493-1]
    NP_690608.1, NM_152869.3 [Q15493-1]
    XP_006724630.1, XM_006724567.2 [Q15493-2]
    UniGeneiHs.77854

    Genome annotation databases

    EnsembliENST00000336169; ENSP00000338400; ENSG00000130988 [Q15493-1]
    ENST00000352078; ENSP00000253303; ENSG00000130988 [Q15493-1]
    ENST00000397180; ENSP00000380365; ENSG00000130988 [Q15493-1]
    ENST00000457380; ENSP00000406568; ENSG00000130988 [Q15493-2]
    GeneIDi9104
    KEGGihsa:9104
    UCSCiuc010nhp.2 human [Q15493-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Similar proteinsi

    Entry informationi

    Entry nameiRGN_HUMAN
    AccessioniPrimary (citable) accession number: Q15493
    Secondary accession number(s): A4FTW1
    , A8K271, Q53FC9, Q5JRR5
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1998
    Last sequence update: November 1, 1996
    Last modified: June 20, 2018
    This is version 143 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Human chromosome X
      Human chromosome X: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    4. SIMILARITY comments
      Index of protein domains and families

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