UniProtKB - Q15464 (SHB_HUMAN)
SH2 domain-containing adapter protein B
SHB
Functioni
Adapter protein which regulates several signal transduction cascades by linking activated receptors to downstream signaling components. May play a role in angiogenesis by regulating FGFR1, VEGFR2 and PDGFR signaling. May also play a role in T-cell antigen receptor/TCR signaling, interleukin-2 signaling, apoptosis and neuronal cells differentiation by mediating basic-FGF and NGF-induced signaling cascades. May also regulate IRS1 and IRS2 signaling in insulin-producing cells.
10 PublicationsGO - Molecular functioni
- phosphotyrosine residue binding Source: CAFA
GO - Biological processi
- angiogenesis Source: UniProtKB-KW
- apoptotic process Source: UniProtKB-KW
- cell differentiation Source: UniProtKB-KW
- signal transduction Source: ProtInc
Keywordsi
Molecular function | Developmental protein |
Biological process | Angiogenesis, Apoptosis, Differentiation |
Enzyme and pathway databases
PathwayCommonsi | Q15464 |
Reactomei | R-HSA-4420097, VEGFA-VEGFR2 Pathway |
SignaLinki | Q15464 |
Names & Taxonomyi
Protein namesi | Recommended name: SH2 domain-containing adapter protein B |
Gene namesi | Name:SHB |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:10838, SHB |
MIMi | 600314, gene |
neXtProti | NX_Q15464 |
VEuPathDBi | HostDB:ENSG00000107338 |
Subcellular locationi
Cytoplasm and Cytosol
Plasma membrane
Note: Associates with membrane lipid rafts upon TCR stimulation.
Cytosol
- cytosol Source: HPA
Nucleus
- nucleoplasm Source: HPA
Plasma Membrane
- plasma membrane Source: UniProtKB-SubCell
Other locations
- cytoplasmic ribonucleoprotein granule Source: HPA
Keywords - Cellular componenti
Cell membrane, Cytoplasm, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 435 | R → K: Loss of interaction with CD3Z. Alters LAT, PLCG1, VAV1 and LCP2 phosphorylation, MAP kinase signaling, Rac1 and JNK activation, intracellular calcium increase, activation of the nuclear factor for activation of T-cells and subsequent interleukin-2 expression which normally occur upon T-cells stimulation. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 6461 |
OpenTargetsi | ENSG00000107338 |
PharmGKBi | PA35744 |
Miscellaneous databases
Pharosi | Q15464, Tbio |
Genetic variation databases
BioMutai | SHB |
DMDMi | 110816415 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000246324 | 1 – 509 | SH2 domain-containing adapter protein BAdd BLAST | 509 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 102 | PhosphoserineCombined sources | 1 | |
Cross-linki | 187 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources | ||
Modified residuei | 307 | PhosphoserineCombined sources | 1 | |
Modified residuei | 317 | PhosphoserineCombined sources | 1 | |
Modified residuei | 388 | PhosphoserineCombined sources | 1 |
Post-translational modificationi
Keywords - PTMi
Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
EPDi | Q15464 |
jPOSTi | Q15464 |
MassIVEi | Q15464 |
MaxQBi | Q15464 |
PaxDbi | Q15464 |
PeptideAtlasi | Q15464 |
PRIDEi | Q15464 |
ProteomicsDBi | 60600 [Q15464-1] 60601 [Q15464-2] |
PTM databases
iPTMneti | Q15464 |
PhosphoSitePlusi | Q15464 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSG00000107338, Expressed in amniotic fluid and 219 other tissues |
Genevisiblei | Q15464, HS |
Organism-specific databases
HPAi | ENSG00000107338, Low tissue specificity |
Interactioni
Subunit structurei
Interacts with PTPN11 (By similarity).
Interacts with phosphorylated 'Tyr-720' of the ligand-activated receptor PDGFRA via its SH2 domain.
Interacts with the ligand-activated receptors PDGFRB, FGFR1, KDR/VEGFR2, IL2RB and IL2RG.
Interacts with EPS8 and V-SRC.
Interacts with GRB2 and GRAP.
Interacts with CD3Z.
Interacts with tyrosine-phosphorylated LAT upon T-cell antigen receptor activation.
Interacts with PLCG1.
Interacts with ZAP70, LCP2/SLP-76, VAV1 and GRAP2.
Interacts with JAK1 and JAK3.
Interacts with PTK2/FAK1.
Interacts with CRK/CrKII.
Interacts with IRS2.
By similarity12 PublicationsBinary interactionsi
Q15464
With | #Exp. | IntAct |
---|---|---|
ABL1 [P00519] | 5 | EBI-4402156,EBI-375543 |
KIT [P10721] | 2 | EBI-4402156,EBI-1379503 |
MET [P08581] | 4 | EBI-4402156,EBI-1039152 |
GO - Molecular functioni
- phosphotyrosine residue binding Source: CAFA
Protein-protein interaction databases
BioGRIDi | 112358, 65 interactors |
IntActi | Q15464, 14 interactors |
MINTi | Q15464 |
STRINGi | 9606.ENSP00000366936 |
Chemistry databases
BindingDBi | Q15464 |
Miscellaneous databases
RNActi | Q15464, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 410 – 504 | SH2PROSITE-ProRule annotationAdd BLAST | 95 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 410 | Mediates interaction with LAT, PTK2/FAK1, JAK1 and JAK32 PublicationsAdd BLAST | 410 | |
Regioni | 1 – 103 | DisorderedSequence analysisAdd BLAST | 103 | |
Regioni | 144 – 178 | DisorderedSequence analysisAdd BLAST | 35 | |
Regioni | 229 – 385 | DisorderedSequence analysisAdd BLAST | 157 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 22 – 36 | Basic and acidic residuesSequence analysisAdd BLAST | 15 | |
Compositional biasi | 41 – 76 | Polar residuesSequence analysisAdd BLAST | 36 | |
Compositional biasi | 146 – 165 | Polar residuesSequence analysisAdd BLAST | 20 | |
Compositional biasi | 232 – 260 | Basic and acidic residuesSequence analysisAdd BLAST | 29 | |
Compositional biasi | 279 – 293 | Polar residuesSequence analysisAdd BLAST | 15 | |
Compositional biasi | 315 – 338 | Basic and acidic residuesSequence analysisAdd BLAST | 24 |
Domaini
Keywords - Domaini
SH2 domainPhylogenomic databases
eggNOGi | ENOG502RT81, Eukaryota |
GeneTreei | ENSGT00940000161591 |
HOGENOMi | CLU_029444_0_0_1 |
InParanoidi | Q15464 |
OMAi | SERKWIG |
OrthoDBi | 1120795at2759 |
PhylomeDBi | Q15464 |
TreeFami | TF325799 |
Family and domain databases
CDDi | cd10389, SH2_SHB, 1 hit |
Gene3Di | 3.30.505.10, 1 hit |
InterProi | View protein in InterPro IPR000980, SH2 IPR036860, SH2_dom_sf IPR035040, SHB IPR035045, SHB_SH2 |
PANTHERi | PTHR15127:SF31, PTHR15127:SF31, 1 hit |
Pfami | View protein in Pfam PF00017, SH2, 1 hit |
PRINTSi | PR00401, SH2DOMAIN |
SMARTi | View protein in SMART SM00252, SH2, 1 hit |
SUPFAMi | SSF55550, SSF55550, 1 hit |
PROSITEi | View protein in PROSITE PS50001, SH2, 1 hit |
s (2)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MAKWLNKYFS LGNSKTKSPP QPPRPDYREQ RRRGERPSQP PQAVPQASSA
60 70 80 90 100
ASASCGPATA SCFSASSGSL PDDSGSTSDL IRAYRAQKER DFEDPYNGPG
110 120 130 140 150
SSLRKLRAMC RLDYCGGSGE PGGVQRAFSA SSASGAAGCC CASSGAGAAA
160 170 180 190 200
SSSSSSGSPH LYRSSSERRP ATPAEVRYIS PKHRLIKVES AAGGGAGDPL
210 220 230 240 250
GGACAGGRTW SPTACGGKKL LNKCAASAAE ESGAGKKDKV TIADDYSDPF
260 270 280 290 300
DAKNDLKSKA GKGESAGYME PYEAQRIMTE FQRQESVRSQ HKGIQLYDTP
310 320 330 340 350
YEPEGQSVDS DSESTVSPRL RESKLPQDDD RPADEYDQPW EWNRVTIPAL
360 370 380 390 400
AAQFNGNEKR QSSPSPSRDR RRQLRAPGGG FKPIKHGSPE FCGILGERVD
410 420 430 440 450
PAVPLEKQIW YHGAISRGDA ENLLRLCKEC SYLVRNSQTS KHDYSLSLRS
460 470 480 490 500
NQGFMHMKLA KTKEKYVLGQ NSPPFDSVPE VIHYYTTRKL PIKGAEHLSL
LYPVAVRTL
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 91 – 93 | DFE → HFQ in CAA53091 (PubMed:8302579).Curated | 3 | |
Sequence conflicti | 347 | I → S in CAA53091 (PubMed:8302579).Curated | 1 | |
Sequence conflicti | 445 | S → P in CAA53091 (PubMed:8302579).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_019846 | 280 – 297 | EFQRQ…GIQLY → GLQEAWRHSPSGCFPVGP in isoform 2. 1 PublicationAdd BLAST | 18 | |
Alternative sequenceiVSP_019847 | 298 – 509 | Missing in isoform 2. 1 PublicationAdd BLAST | 212 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X75342 mRNA Translation: CAA53091.1 Different initiation. AL138752 Genomic DNA No translation available. AL161448 Genomic DNA No translation available. AL583849 Genomic DNA No translation available. CH471071 Genomic DNA Translation: EAW58254.1 CH471071 Genomic DNA Translation: EAW58255.1 BC094765 mRNA Translation: AAH94765.1 Different initiation. BC136581 mRNA Translation: AAI36582.1 BC136582 mRNA Translation: AAI36583.1 |
CCDSi | CCDS43806.1 [Q15464-1] |
PIRi | I38228 |
RefSeqi | NP_003019.2, NM_003028.2 [Q15464-1] |
Genome annotation databases
Ensembli | ENST00000377707; ENSP00000366936; ENSG00000107338 |
GeneIDi | 6461 |
KEGGi | hsa:6461 |
MANE-Selecti | ENST00000377707.4; ENSP00000366936.3; NM_003028.3; NP_003019.2 |
UCSCi | uc004aax.4, human [Q15464-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X75342 mRNA Translation: CAA53091.1 Different initiation. AL138752 Genomic DNA No translation available. AL161448 Genomic DNA No translation available. AL583849 Genomic DNA No translation available. CH471071 Genomic DNA Translation: EAW58254.1 CH471071 Genomic DNA Translation: EAW58255.1 BC094765 mRNA Translation: AAH94765.1 Different initiation. BC136581 mRNA Translation: AAI36582.1 BC136582 mRNA Translation: AAI36583.1 |
CCDSi | CCDS43806.1 [Q15464-1] |
PIRi | I38228 |
RefSeqi | NP_003019.2, NM_003028.2 [Q15464-1] |
3D structure databases
SMRi | Q15464 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 112358, 65 interactors |
IntActi | Q15464, 14 interactors |
MINTi | Q15464 |
STRINGi | 9606.ENSP00000366936 |
Chemistry databases
BindingDBi | Q15464 |
PTM databases
iPTMneti | Q15464 |
PhosphoSitePlusi | Q15464 |
Genetic variation databases
BioMutai | SHB |
DMDMi | 110816415 |
Proteomic databases
EPDi | Q15464 |
jPOSTi | Q15464 |
MassIVEi | Q15464 |
MaxQBi | Q15464 |
PaxDbi | Q15464 |
PeptideAtlasi | Q15464 |
PRIDEi | Q15464 |
ProteomicsDBi | 60600 [Q15464-1] 60601 [Q15464-2] |
Protocols and materials databases
Antibodypediai | 4119, 287 antibodies from 30 providers |
DNASUi | 6461 |
Genome annotation databases
Ensembli | ENST00000377707; ENSP00000366936; ENSG00000107338 |
GeneIDi | 6461 |
KEGGi | hsa:6461 |
MANE-Selecti | ENST00000377707.4; ENSP00000366936.3; NM_003028.3; NP_003019.2 |
UCSCi | uc004aax.4, human [Q15464-1] |
Organism-specific databases
CTDi | 6461 |
DisGeNETi | 6461 |
GeneCardsi | SHB |
HGNCi | HGNC:10838, SHB |
HPAi | ENSG00000107338, Low tissue specificity |
MIMi | 600314, gene |
neXtProti | NX_Q15464 |
OpenTargetsi | ENSG00000107338 |
PharmGKBi | PA35744 |
VEuPathDBi | HostDB:ENSG00000107338 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | ENOG502RT81, Eukaryota |
GeneTreei | ENSGT00940000161591 |
HOGENOMi | CLU_029444_0_0_1 |
InParanoidi | Q15464 |
OMAi | SERKWIG |
OrthoDBi | 1120795at2759 |
PhylomeDBi | Q15464 |
TreeFami | TF325799 |
Enzyme and pathway databases
PathwayCommonsi | Q15464 |
Reactomei | R-HSA-4420097, VEGFA-VEGFR2 Pathway |
SignaLinki | Q15464 |
Miscellaneous databases
BioGRID-ORCSi | 6461, 18 hits in 1047 CRISPR screens |
ChiTaRSi | SHB, human |
GeneWikii | SHB_(gene) |
GenomeRNAii | 6461 |
Pharosi | Q15464, Tbio |
PROi | PR:Q15464 |
RNActi | Q15464, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000107338, Expressed in amniotic fluid and 219 other tissues |
Genevisiblei | Q15464, HS |
Family and domain databases
CDDi | cd10389, SH2_SHB, 1 hit |
Gene3Di | 3.30.505.10, 1 hit |
InterProi | View protein in InterPro IPR000980, SH2 IPR036860, SH2_dom_sf IPR035040, SHB IPR035045, SHB_SH2 |
PANTHERi | PTHR15127:SF31, PTHR15127:SF31, 1 hit |
Pfami | View protein in Pfam PF00017, SH2, 1 hit |
PRINTSi | PR00401, SH2DOMAIN |
SMARTi | View protein in SMART SM00252, SH2, 1 hit |
SUPFAMi | SSF55550, SSF55550, 1 hit |
PROSITEi | View protein in PROSITE PS50001, SH2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | SHB_HUMAN | |
Accessioni | Q15464Primary (citable) accession number: Q15464 Secondary accession number(s): B9EGM0 Q5VUM8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 25, 2006 |
Last sequence update: | July 25, 2006 | |
Last modified: | February 23, 2022 | |
This is version 165 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human chromosome 9
Human chromosome 9: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot