Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Cytohesin-1

Gene

CYTH1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Promotes guanine-nucleotide exchange on ARF1, ARF5 and ARF6. Promotes the activation of ARF factors through replacement of GDP with GTP. Plays an important role in membrane trafficking, during junctional remodeling and epithelial polarization, through regulation of ARF6 activity.By similarity3 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei281Phosphatidylinositol 3,4,5-trisphosphateBy similarity1
Binding sitei292Phosphatidylinositol 3,4,5-trisphosphateBy similarity1
Binding sitei302Phosphatidylinositol 3,4,5-trisphosphateBy similarity1
Binding sitei351Phosphatidylinositol 3,4,5-trisphosphateBy similarity1

GO - Molecular functioni

  • ARF guanyl-nucleotide exchange factor activity Source: UniProtKB
  • lipid binding Source: UniProtKB-KW

GO - Biological processi

  • establishment of epithelial cell polarity Source: UniProtKB
  • regulation of ARF protein signal transduction Source: InterPro
  • regulation of cell adhesion Source: MGI
  • vesicle-mediated transport Source: ProtInc

Keywordsi

Molecular functionGuanine-nucleotide releasing factor
LigandLipid-binding

Enzyme and pathway databases

ReactomeiR-HSA-6811438 Intra-Golgi traffic
SIGNORiQ15438

Names & Taxonomyi

Protein namesi
Recommended name:
Cytohesin-1
Alternative name(s):
PH, SEC7 and coiled-coil domain-containing protein 1
SEC7 homolog B2-1
Gene namesi
Name:CYTH1Imported
Synonyms:D17S811E, PSCD1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 17

Organism-specific databases

EuPathDBiHostDB:ENSG00000108669.16
HGNCiHGNC:9501 CYTH1
MIMi182115 gene
neXtProtiNX_Q15438

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell junction, Cell membrane, Cytoplasm, Membrane, Tight junction

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi157E → A or K: Reduces guanine exchange factor activity by over 90%. 1 Publication1
Mutagenesisi187Y → A: Reduces guanine exchange factor activity by over 90%. 1 Publication1
Mutagenesisi195M → A: Reduces guanine exchange factor activity by over 90%. 1 Publication1

Organism-specific databases

DisGeNETi9267
OpenTargetsiENSG00000108669
PharmGKBiPA164718528

Polymorphism and mutation databases

BioMutaiCYTH1
DMDMi2498175

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001201941 – 398Cytohesin-1Add BLAST398

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineCombined sources1

Post-translational modificationi

Ubiquitinated by SCF(FBXW11) E3 ubiquitin-protein ligase complex. Ubiquitination induces proteasomal degradation.1 Publication

Keywords - PTMi

Acetylation, Ubl conjugation

Proteomic databases

EPDiQ15438
MaxQBiQ15438
PaxDbiQ15438
PeptideAtlasiQ15438
PRIDEiQ15438
ProteomicsDBi60597
60598 [Q15438-2]

PTM databases

iPTMnetiQ15438
PhosphoSitePlusiQ15438

Expressioni

Tissue specificityi

Ubiquitous.

Gene expression databases

BgeeiENSG00000108669
CleanExiHS_CYTH1
ExpressionAtlasiQ15438 baseline and differential
GenevisibleiQ15438 HS

Organism-specific databases

HPAiHPA047498

Interactioni

Subunit structurei

Interacts with TRIM23 and CYTIP (PubMed:10748148, PubMed:11867758). Interacts (via coiled-coil domain) with FRMD4A (via coiled-coil domain) (By similarity). Interacts with FRMD4B (By similarity). Found in a complex with PARD3, CYTH1 and FRMD4A (By similarity). Interacts (via N-terminal domain) with INAVA (via N-terminal domain) (PubMed:29420262).By similarity3 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

  • ARF guanyl-nucleotide exchange factor activity Source: UniProtKB

Protein-protein interaction databases

BioGridi114688, 21 interactors
IntActiQ15438, 13 interactors
MINTiQ15438
STRINGi9606.ENSP00000354398

Structurei

Secondary structure

1398
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi64 – 75Combined sources12
Helixi77 – 86Combined sources10
Helixi94 – 103Combined sources10
Helixi109 – 116Combined sources8
Helixi121 – 133Combined sources13
Turni137 – 139Combined sources3
Helixi141 – 150Combined sources10
Helixi158 – 175Combined sources18
Helixi183 – 201Combined sources19
Beta strandi203 – 205Combined sources3
Helixi211 – 217Combined sources7
Turni218 – 221Combined sources4
Helixi229 – 241Combined sources13
Beta strandi246 – 248Combined sources3

3D structure databases

ProteinModelPortaliQ15438
SMRiQ15438
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ15438

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini73 – 202SEC7PROSITE-ProRule annotationAdd BLAST130
Domaini260 – 377PHPROSITE-ProRule annotationAdd BLAST118

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni269 – 277Phosphatidylinositol 3,4,5-trisphosphate bindingBy similarity9
Regioni388 – 396C-terminal autoinhibitory regionBy similarity9

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili10 – 67Sequence analysisAdd BLAST58

Domaini

Binds via its PH domain to the inositol head group of phosphatidylinositol 3,4,5-trisphosphate.By similarity
Autoinhibited by its C-terminal basic region.By similarity

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG0930 Eukaryota
COG5307 LUCA
GeneTreeiENSGT00760000119036
HOGENOMiHOG000253023
HOVERGENiHBG002647
InParanoidiQ15438
KOiK18441
OMAiEDWIKCI
OrthoDBiEOG091G0RZS
PhylomeDBiQ15438
TreeFamiTF352091

Family and domain databases

Gene3Di1.10.1000.11, 1 hit
2.30.29.30, 1 hit
InterProiView protein in InterPro
IPR011993 PH-like_dom_sf
IPR001849 PH_domain
IPR023394 Sec7_C_sf
IPR000904 Sec7_dom
IPR035999 Sec7_dom_sf
PfamiView protein in Pfam
PF00169 PH, 1 hit
PF01369 Sec7, 1 hit
SMARTiView protein in SMART
SM00233 PH, 1 hit
SM00222 Sec7, 1 hit
SUPFAMiSSF48425 SSF48425, 1 hit
PROSITEiView protein in PROSITE
PS50003 PH_DOMAIN, 1 hit
PS50190 SEC7, 1 hit

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q15438-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEEDDSYVPS DLTAEERQEL ENIRRRKQEL LADIQRLKDE IAEVANEIEN
60 70 80 90 100
LGSTEERKNM QRNKQVAMGR KKFNMDPKKG IQFLIENDLL KNTCEDIAQF
110 120 130 140 150
LYKGEGLNKT AIGDYLGERD EFNIQVLHAF VELHEFTDLN LVQALRQFLW
160 170 180 190 200
SFRLPGEAQK IDRMMEAFAQ RYCQCNNGVF QSTDTCYVLS FAIIMLNTSL
210 220 230 240 250
HNPNVKDKPT VERFIAMNRG INDGGDLPEE LLRNLYESIK NEPFKIPEDD
260 270 280 290 300
GNDLTHTFFN PDREGWLLKL GGGRVKTWKR RWFILTDNCL YYFEYTTDKE
310 320 330 340 350
PRGIIPLENL SIREVEDSKK PNCFELYIPD NKDQVIKACK TEADGRVVEG
360 370 380 390
NHTVYRISAP TPEEKEEWIK CIKAAISRDP FYEMLAARKK KVSSTKRH
Length:398
Mass (Da):46,413
Last modified:November 1, 1996 - v1
Checksum:i067FEE0FEA7A4C86
GO
Isoform 2 (identifier: Q15438-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     273-273: Missing.

Show »
Length:397
Mass (Da):46,356
Checksum:iCBD984BC348E6B15
GO
Isoform 3 (identifier: Q15438-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-59: Missing.

Note: No experimental confirmation available.
Show »
Length:339
Mass (Da):39,470
Checksum:iC08318AD7469BD02
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti353 – 361TVYRISAPT → MFTGSQLRR in AAF37737 (PubMed:15489334).Curated9
Sequence conflicti353 – 361TVYRISAPT → MFTGSQLRR in AAF37738 (PubMed:15489334).Curated9

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0558841 – 59Missing in isoform 3. 1 PublicationAdd BLAST59
Alternative sequenceiVSP_006034273Missing in isoform 2. Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M85169 mRNA Translation: AAA36602.1
AK293894 mRNA Translation: BAH11620.1
AK316277 mRNA Translation: BAH14648.1
AC022966 Genomic DNA No translation available.
AC099804 Genomic DNA No translation available.
BC038385 mRNA Translation: AAH38385.1
BC050452 mRNA Translation: AAH50452.1
AF125362
, AF125350, AF125351, AF125352, AF125353, AF125354, AF125355, AF125356, AF125357, AF125359, AF125360, AF125361 Genomic DNA Translation: AAF37737.1
AF125362
, AF125350, AF125351, AF125352, AF125353, AF125354, AF125355, AF125356, AF125357, AF125358, AF125359, AF125360, AF125361 Genomic DNA Translation: AAF37738.1
CCDSiCCDS32754.1 [Q15438-2]
CCDS42392.2 [Q15438-1]
PIRiS24168
RefSeqiNP_001278947.1, NM_001292018.1 [Q15438-3]
NP_001278948.1, NM_001292019.1 [Q15438-3]
NP_004753.1, NM_004762.3 [Q15438-1]
NP_059430.2, NM_017456.3 [Q15438-2]
XP_011523779.1, XM_011525477.1 [Q15438-3]
UniGeneiHs.191215

Genome annotation databases

EnsembliENST00000361101; ENSP00000354398; ENSG00000108669 [Q15438-1]
ENST00000446868; ENSP00000389095; ENSG00000108669 [Q15438-1]
ENST00000585509; ENSP00000465940; ENSG00000108669 [Q15438-3]
ENST00000589297; ENSP00000466512; ENSG00000108669 [Q15438-3]
ENST00000591455; ENSP00000465665; ENSG00000108669 [Q15438-2]
GeneIDi9267
KEGGihsa:9267
UCSCiuc002jvw.4 human [Q15438-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiCYH1_HUMAN
AccessioniPrimary (citable) accession number: Q15438
Secondary accession number(s): A6NFW7
, B7Z1T4, Q9P123, Q9P124
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: July 18, 2018
This is version 172 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health