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Protein

Scaffold attachment factor B1

Gene

SAFB

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Binds to scaffold/matrix attachment region (S/MAR) DNA and forms a molecular assembly point to allow the formation of a 'transcriptosomal' complex (consisting of SR proteins and RNA polymerase II) coupling transcription and RNA processing (By similarity). Can function as an estrogen receptor corepressor and can also bind to the HSP27 promoter and decrease its transcription. When associated with RBMX, binds to and stimulates transcription from the SREBF1 promoter (By similarity). Can inhibit cell proliferation.By similarity

GO - Molecular functioni

  • chromatin binding Source: UniProtKB
  • double-stranded DNA binding Source: ProtInc
  • RNA binding Source: UniProtKB
  • RNA polymerase II proximal promoter sequence-specific DNA binding Source: UniProtKB
  • sequence-specific DNA binding Source: GO_Central

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, Repressor, RNA-binding
Biological processTranscription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-HSA-3899300 SUMOylation of transcription cofactors

Names & Taxonomyi

Protein namesi
Recommended name:
Scaffold attachment factor B1
Short name:
SAF-B
Short name:
SAF-B1
Alternative name(s):
HSP27 estrogen response element-TATA box-binding protein
Short name:
HSP27 ERE-TATA-binding protein
Gene namesi
Name:SAFB
Synonyms:HAP, HET, SAFB1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 19

Organism-specific databases

EuPathDBiHostDB:ENSG00000160633.12
HGNCiHGNC:10520 SAFB
MIMi602895 gene
neXtProtiNX_Q15424

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi6294
OpenTargetsiENSG00000160633
PharmGKBiPA34928

Polymorphism and mutation databases

BioMutaiSAFB
DMDMi116242782

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources1 Publication
ChainiPRO_00000819052 – 915Scaffold attachment factor B1Add BLAST914

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineCombined sources1 Publication1
Modified residuei24PhosphoserineBy similarity1
Modified residuei55PhosphoserineCombined sources1
Modified residuei79PhosphoserineCombined sources1
Cross-linki172Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki186Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei188PhosphothreonineCombined sources1
Modified residuei195PhosphoserineCombined sources1
Modified residuei197PhosphoserineCombined sources1
Modified residuei209PhosphoserineBy similarity1
Cross-linki231Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)1 Publication
Cross-linki294Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)1 Publication
Cross-linki381Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei383PhosphoserineCombined sources1
Modified residuei384PhosphoserineCombined sources1
Cross-linki392Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei415PhosphoserineCombined sources1
Cross-linki483Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki514Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki543Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki570Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki578Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1); alternateCombined sources
Cross-linki578Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateCombined sources
Modified residuei580PhosphoserineCombined sources1
Modified residuei582PhosphoserineCombined sources1
Modified residuei601PhosphoserineCombined sources1
Modified residuei604PhosphoserineCombined sources1
Modified residuei607N6-acetyllysineCombined sources1
Modified residuei811Omega-N-methylarginineCombined sources1
Cross-linki847Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei868Asymmetric dimethylarginineCombined sources1
Modified residuei874Asymmetric dimethylarginineCombined sources1
Modified residuei884Asymmetric dimethylarginineCombined sources1

Post-translational modificationi

Sumoylated by PIAS1 with SUMO1 and SUMO2/3, desumoylated by SENP1. Sumoylation is required for transcriptional repressor activity.1 Publication

Keywords - PTMi

Acetylation, Isopeptide bond, Methylation, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ15424
MaxQBiQ15424
PaxDbiQ15424
PeptideAtlasiQ15424
PRIDEiQ15424
ProteomicsDBi60586

PTM databases

iPTMnetiQ15424
PhosphoSitePlusiQ15424
SwissPalmiQ15424

Expressioni

Tissue specificityi

Ubiquitous. Expressed at high levels in the CNS and at low levels in the liver. Expressed in a wide number of breast cancer cell lines.

Gene expression databases

BgeeiENSG00000160633 Expressed in 232 organ(s), highest expression level in corpus callosum
CleanExiHS_SAFB
ExpressionAtlasiQ15424 baseline and differential
GenevisibleiQ15424 HS

Organism-specific databases

HPAiCAB001969
HPA016832
HPA020076

Interactioni

Subunit structurei

Monomer. Can form homodimers. Interacts with KHDRBS3, POLR2A, SAFB2 or SFRS1, SFRS9 and TRA2B/SFRS10 (By similarity). Interacts with isoform 1 and isoform 2 of SRPK1 and inhibits its activity. Interacts with RBMX (By similarity).By similarity

Binary interactionsi

Protein-protein interaction databases

BioGridi112201, 105 interactors
IntActiQ15424, 39 interactors
MINTiQ15424
STRINGi9606.ENSP00000292123

Structurei

3D structure databases

ProteinModelPortaliQ15424
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini31 – 65SAPPROSITE-ProRule annotationAdd BLAST35
Domaini406 – 484RRMPROSITE-ProRule annotationAdd BLAST79

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni528 – 792Interaction with POLR2A1 PublicationAdd BLAST265
Regioni599 – 915Interaction with SAFB2Add BLAST317

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi599 – 616Nuclear localization signalSequence analysisAdd BLAST18

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi612 – 831Arg-richAdd BLAST220
Compositional biasi625 – 705Glu-richAdd BLAST81
Compositional biasi785 – 903Gly-richAdd BLAST119

Phylogenomic databases

eggNOGiKOG4661 Eukaryota
ENOG4111F1G LUCA
GeneTreeiENSGT00730000110777
HOGENOMiHOG000092533
HOVERGENiHBG078408
InParanoidiQ15424
OMAiPNDARFT
OrthoDBiEOG091G0GPT
PhylomeDBiQ15424
TreeFamiTF325240

Family and domain databases

Gene3Di1.10.720.30, 1 hit
3.30.70.330, 1 hit
InterProiView protein in InterPro
IPR012677 Nucleotide-bd_a/b_plait_sf
IPR035979 RBD_domain_sf
IPR000504 RRM_dom
IPR034781 SAFB1
IPR003034 SAP_dom
IPR036361 SAP_dom_sf
PANTHERiPTHR15683:SF6 PTHR15683:SF6, 1 hit
PfamiView protein in Pfam
PF00076 RRM_1, 1 hit
PF02037 SAP, 1 hit
SMARTiView protein in SMART
SM00360 RRM, 1 hit
SM00513 SAP, 1 hit
SUPFAMiSSF54928 SSF54928, 1 hit
SSF68906 SSF68906, 1 hit
PROSITEiView protein in PROSITE
PS50102 RRM, 1 hit
PS50800 SAP, 1 hit

Sequences (4+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 4 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q15424-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MAETLSGLGD SGAAGAAALS SASSETGTRR LSDLRVIDLR AELRKRNVDS
60 70 80 90 100
SGNKSVLMER LKKAIEDEGG NPDEIEITSE GNKKTSKRSS KGRKPEEEGV
110 120 130 140 150
EDNGLEENSG DGQEDVETSL ENLQDIDIMD ISVLDEAEID NGSVADCVED
160 170 180 190 200
DDADNLQESL SDSRELVEGE MKELPEQLQE HAIEDKETIN NLDTSSSDFT
210 220 230 240 250
ILQEIEEPSL EPENEKILDI LGETCKSEPV KEESSELEQP FAQDTSSVGP
260 270 280 290 300
DRKLAEEEDL FDSAHPEEGD LDLASESTAH AQSSKADSLL AVVKREPAEQ
310 320 330 340 350
PGDGERTDCE PVGLEPAVEQ SSAASELAEA SSEELAEAPT EAPSPEARDS
360 370 380 390 400
KEDGRKFDFD ACNEVPPAPK ESSTSEGADQ KMSSPEDDSD TKRLSKEEKG
410 420 430 440 450
RSSCGRNFWV SGLSSTTRAT DLKNLFSKYG KVVGAKVVTN ARSPGARCYG
460 470 480 490 500
FVTMSTAEEA TKCINHLHKT ELHGKMISVE KAKNEPVGKK TSDKRDSDGK
510 520 530 540 550
KEKSSNSDRS TNLKRDDKCD RKDDAKKGDD GSGEKSKDQD DQKPGPSERS
560 570 580 590 600
RATKSGSRGT ERTVVMDKSK GVPVISVKTS GSKERASKSQ DRKSASREKR
610 620 630 640 650
SVVSFDKVKE PRKSRDSESH SRVRERSERE QRMQAQWERE ERERLEIARE
660 670 680 690 700
RLAFQRQRLE RERMERERLE RERMHVEHER RREQERIHRE REELRRQQEL
710 720 730 740 750
RYEQERRPAV RRPYDLDRRD DAYWPEAKRA ALDERYHSDF NRQDRFHDFD
760 770 780 790 800
HRDRGRYPDH SVDRREGSRS MMGEREGQHY PERHGGPERH GRDSRDGWGG
810 820 830 840 850
YGSDKRMSEG RGLPPPPRRD WGDHGRREDD RSWQGTADGG MMDRDHKRWQ
860 870 880 890 900
GGERSMSGHS GPGHMMNRGG MSGRGSFAPG GASRGHPIPH GGMQGGFGGQ
910
SRGSRPSDAR FTRRY
Length:915
Mass (Da):102,642
Last modified:October 17, 2006 - v4
Checksum:iE865940BC7783C4A
GO
Isoform 2 (identifier: Q15424-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     114-182: Missing.
     817-817: P → PRG

Note: No experimental confirmation available.
Show »
Length:848
Mass (Da):95,181
Checksum:i681FC59B003DBAD5
GO
Isoform 3 (identifier: Q15424-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     817-817: P → PRG

Note: No experimental confirmation available.
Show »
Length:917
Mass (Da):102,855
Checksum:i9DB77C0D393EA6BB
GO
Isoform 4 (identifier: Q15424-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     621-621: Missing.
     817-817: P → PRG

Note: No experimental confirmation available.
Show »
Length:916
Mass (Da):102,768
Checksum:i6A3B946D0D5A0733
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
K7EII0K7EII0_HUMAN
Scaffold attachment factor B1
SAFB
153Annotation score:
K7ES42K7ES42_HUMAN
Scaffold attachment factor B1
SAFB
106Annotation score:

Sequence cautioni

The sequence BAD92017 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti16A → P in AAC00056 (PubMed:9328833).Curated1
Sequence conflicti42 – 43EL → DV in AAC00056 (PubMed:9328833).Curated2
Sequence conflicti197S → P in BAH12852 (PubMed:14702039).Curated1
Sequence conflicti463C → Y in BAH12852 (PubMed:14702039).Curated1
Sequence conflicti692E → V in BAH12852 (PubMed:14702039).Curated1
Sequence conflicti731A → G in AAC00056 (PubMed:9328833).Curated1
Sequence conflicti731A → G in AAC18697 (PubMed:8600450).Curated1
Sequence conflicti744D → E in AAC00056 (PubMed:9328833).Curated1
Sequence conflicti744D → E in AAC18697 (PubMed:8600450).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_045838114 – 182Missing in isoform 2. 1 PublicationAdd BLAST69
Alternative sequenceiVSP_046902621Missing in isoform 4. 2 Publications1
Alternative sequenceiVSP_045839817P → PRG in isoform 2, isoform 3 and isoform 4. 3 Publications1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U72355 mRNA Translation: AAC00056.1
AK298707 mRNA Translation: BAH12852.1
AC004611 Genomic DNA Translation: AAC14667.1
AC011499 Genomic DNA No translation available.
AC134303 Genomic DNA No translation available.
BC126219 mRNA Translation: AAI26220.1
BC143937 mRNA Translation: AAI43938.1
BC143939 mRNA Translation: AAI43940.1
AB208780 mRNA Translation: BAD92017.1 Different initiation.
L43631 mRNA Translation: AAC18697.1
CCDSiCCDS12142.1 [Q15424-1]
CCDS56077.1 [Q15424-2]
CCDS59339.1 [Q15424-3]
CCDS59340.1 [Q15424-4]
PIRiS64732
RefSeqiNP_001188267.1, NM_001201338.1 [Q15424-3]
NP_001188268.1, NM_001201339.1 [Q15424-4]
NP_001188269.1, NM_001201340.1 [Q15424-2]
NP_001307500.1, NM_001320571.1
NP_001307501.1, NM_001320572.1
NP_002958.2, NM_002967.3 [Q15424-1]
UniGeneiHs.728802

Genome annotation databases

EnsembliENST00000292123; ENSP00000292123; ENSG00000160633 [Q15424-1]
ENST00000454510; ENSP00000415895; ENSG00000160633 [Q15424-2]
ENST00000588852; ENSP00000467423; ENSG00000160633 [Q15424-3]
ENST00000592224; ENSP00000464840; ENSG00000160633 [Q15424-4]
GeneIDi6294
KEGGihsa:6294
UCSCiuc002mce.5 human [Q15424-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U72355 mRNA Translation: AAC00056.1
AK298707 mRNA Translation: BAH12852.1
AC004611 Genomic DNA Translation: AAC14667.1
AC011499 Genomic DNA No translation available.
AC134303 Genomic DNA No translation available.
BC126219 mRNA Translation: AAI26220.1
BC143937 mRNA Translation: AAI43938.1
BC143939 mRNA Translation: AAI43940.1
AB208780 mRNA Translation: BAD92017.1 Different initiation.
L43631 mRNA Translation: AAC18697.1
CCDSiCCDS12142.1 [Q15424-1]
CCDS56077.1 [Q15424-2]
CCDS59339.1 [Q15424-3]
CCDS59340.1 [Q15424-4]
PIRiS64732
RefSeqiNP_001188267.1, NM_001201338.1 [Q15424-3]
NP_001188268.1, NM_001201339.1 [Q15424-4]
NP_001188269.1, NM_001201340.1 [Q15424-2]
NP_001307500.1, NM_001320571.1
NP_001307501.1, NM_001320572.1
NP_002958.2, NM_002967.3 [Q15424-1]
UniGeneiHs.728802

3D structure databases

ProteinModelPortaliQ15424
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi112201, 105 interactors
IntActiQ15424, 39 interactors
MINTiQ15424
STRINGi9606.ENSP00000292123

PTM databases

iPTMnetiQ15424
PhosphoSitePlusiQ15424
SwissPalmiQ15424

Polymorphism and mutation databases

BioMutaiSAFB
DMDMi116242782

Proteomic databases

EPDiQ15424
MaxQBiQ15424
PaxDbiQ15424
PeptideAtlasiQ15424
PRIDEiQ15424
ProteomicsDBi60586

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000292123; ENSP00000292123; ENSG00000160633 [Q15424-1]
ENST00000454510; ENSP00000415895; ENSG00000160633 [Q15424-2]
ENST00000588852; ENSP00000467423; ENSG00000160633 [Q15424-3]
ENST00000592224; ENSP00000464840; ENSG00000160633 [Q15424-4]
GeneIDi6294
KEGGihsa:6294
UCSCiuc002mce.5 human [Q15424-1]

Organism-specific databases

CTDi6294
DisGeNETi6294
EuPathDBiHostDB:ENSG00000160633.12
GeneCardsiSAFB
H-InvDBiHIX0014682
HGNCiHGNC:10520 SAFB
HPAiCAB001969
HPA016832
HPA020076
MIMi602895 gene
neXtProtiNX_Q15424
OpenTargetsiENSG00000160633
PharmGKBiPA34928
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG4661 Eukaryota
ENOG4111F1G LUCA
GeneTreeiENSGT00730000110777
HOGENOMiHOG000092533
HOVERGENiHBG078408
InParanoidiQ15424
OMAiPNDARFT
OrthoDBiEOG091G0GPT
PhylomeDBiQ15424
TreeFamiTF325240

Enzyme and pathway databases

ReactomeiR-HSA-3899300 SUMOylation of transcription cofactors

Miscellaneous databases

ChiTaRSiSAFB human
GeneWikiiSAFB
GenomeRNAii6294
PROiPR:Q15424
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000160633 Expressed in 232 organ(s), highest expression level in corpus callosum
CleanExiHS_SAFB
ExpressionAtlasiQ15424 baseline and differential
GenevisibleiQ15424 HS

Family and domain databases

Gene3Di1.10.720.30, 1 hit
3.30.70.330, 1 hit
InterProiView protein in InterPro
IPR012677 Nucleotide-bd_a/b_plait_sf
IPR035979 RBD_domain_sf
IPR000504 RRM_dom
IPR034781 SAFB1
IPR003034 SAP_dom
IPR036361 SAP_dom_sf
PANTHERiPTHR15683:SF6 PTHR15683:SF6, 1 hit
PfamiView protein in Pfam
PF00076 RRM_1, 1 hit
PF02037 SAP, 1 hit
SMARTiView protein in SMART
SM00360 RRM, 1 hit
SM00513 SAP, 1 hit
SUPFAMiSSF54928 SSF54928, 1 hit
SSF68906 SSF68906, 1 hit
PROSITEiView protein in PROSITE
PS50102 RRM, 1 hit
PS50800 SAP, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiSAFB1_HUMAN
AccessioniPrimary (citable) accession number: Q15424
Secondary accession number(s): A0AV56
, B7Z5B6, B7ZLP6, F5H0H3, O60406, Q59HH8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 14, 2003
Last sequence update: October 17, 2006
Last modified: November 7, 2018
This is version 178 of the entry and version 4 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  2. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
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