UniProtKB - Q15417 (CNN3_HUMAN)
Protein
Calponin-3
Gene
CNN3
Organism
Homo sapiens (Human)
Status
Functioni
Thin filament-associated protein that is implicated in the regulation and modulation of smooth muscle contraction. It is capable of binding to actin, calmodulin and tropomyosin. The interaction of calponin with actin inhibits the actomyosin Mg-ATPase activity.
GO - Molecular functioni
- actin binding Source: UniProtKB-KW
- cadherin binding involved in cell-cell adhesion Source: BHF-UCL
- calmodulin binding Source: UniProtKB-KW
- microtubule binding Source: Ensembl
GO - Biological processi
- actomyosin structure organization Source: InterPro
- epithelial cell differentiation Source: UniProtKB
- negative regulation of ATPase activity Source: Ensembl
Keywordsi
Molecular function | Actin-binding, Calmodulin-binding |
Enzyme and pathway databases
PathwayCommonsi | Q15417 |
SIGNORi | Q15417 |
Names & Taxonomyi
Protein namesi | Recommended name: Calponin-3Alternative name(s): Calponin, acidic isoform |
Gene namesi | Name:CNN3 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:2157, CNN3 |
MIMi | 602374, gene |
neXtProti | NX_Q15417 |
VEuPathDBi | HostDB:ENSG00000117519.15 |
Subcellular locationi
Cytoskeleton
- actin cytoskeleton Source: HPA
Cytosol
- cytosol Source: HPA
Other locations
- adherens junction Source: BHF-UCL
- dendritic spine Source: Ensembl
- focal adhesion Source: UniProtKB
- neuronal cell body Source: Ensembl
- postsynaptic density Source: Ensembl
Pathology & Biotechi
Organism-specific databases
DisGeNETi | 1266 |
OpenTargetsi | ENSG00000117519 |
PharmGKBi | PA26667 |
Miscellaneous databases
Pharosi | Q15417, Tbio |
Genetic variation databases
BioMutai | CNN3 |
DMDMi | 6225157 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000204776 | 1 – 329 | Calponin-3Add BLAST | 329 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 23 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 158 | N6-methyllysineCombined sources | 1 | |
Modified residuei | 323 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
Acetylation, Methylation, PhosphoproteinProteomic databases
EPDi | Q15417 |
jPOSTi | Q15417 |
MassIVEi | Q15417 |
PaxDbi | Q15417 |
PeptideAtlasi | Q15417 |
PRIDEi | Q15417 |
ProteomicsDBi | 30452 4050 60582 [Q15417-1] |
PTM databases
GlyConnecti | 1059, 6 N-Linked glycans (1 site) |
GlyGeni | Q15417, 1 site |
iPTMneti | Q15417 |
MetOSitei | Q15417 |
PhosphoSitePlusi | Q15417 |
SwissPalmi | Q15417 |
Expressioni
Tissue specificityi
Expressed in both non-smooth muscle tissues as well as smooth muscle tissues.
Gene expression databases
Bgeei | ENSG00000117519, Expressed in ventricular zone and 243 other tissues |
ExpressionAtlasi | Q15417, baseline and differential |
Genevisiblei | Q15417, HS |
Organism-specific databases
HPAi | ENSG00000117519, Low tissue specificity |
Interactioni
GO - Molecular functioni
- actin binding Source: UniProtKB-KW
- cadherin binding involved in cell-cell adhesion Source: BHF-UCL
- calmodulin binding Source: UniProtKB-KW
- microtubule binding Source: Ensembl
Protein-protein interaction databases
BioGRIDi | 107666, 28 interactors |
CORUMi | Q15417 |
IntActi | Q15417, 17 interactors |
MINTi | Q15417 |
STRINGi | 9606.ENSP00000359225 |
Miscellaneous databases
RNActi | Q15417, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 26 – 130 | Calponin-homology (CH)PROSITE-ProRule annotationAdd BLAST | 105 | |
Repeati | 164 – 189 | Calponin-like 1Add BLAST | 26 | |
Repeati | 204 – 229 | Calponin-like 2Add BLAST | 26 | |
Repeati | 243 – 268 | Calponin-like 3Add BLAST | 26 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 290 – 329 | Asp/Glu-rich (acidic)Add BLAST | 40 |
Sequence similaritiesi
Belongs to the calponin family.Curated
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | KOG2046, Eukaryota |
GeneTreei | ENSGT00940000154539 |
HOGENOMi | CLU_055232_0_2_1 |
InParanoidi | Q15417 |
OMAi | QDDYPRE |
OrthoDBi | 861989at2759 |
PhylomeDBi | Q15417 |
TreeFami | TF313921 |
Family and domain databases
CDDi | cd00014, CH, 1 hit |
Gene3Di | 1.10.418.10, 1 hit |
InterProi | View protein in InterPro IPR001997, Calponin/LIMCH1 IPR000557, Calponin_repeat IPR001715, CH-domain IPR036872, CH_dom_sf IPR003096, SM22_calponin |
Pfami | View protein in Pfam PF00402, Calponin, 3 hits PF00307, CH, 1 hit |
PRINTSi | PR00889, CALPONIN PR00888, SM22CALPONIN |
SMARTi | View protein in SMART SM00033, CH, 1 hit |
SUPFAMi | SSF47576, SSF47576, 1 hit |
PROSITEi | View protein in PROSITE PS01052, CALPONIN_1, 3 hits PS51122, CALPONIN_2, 3 hits PS50021, CH, 1 hit |
s (3+)i Sequence
Sequence statusi: Complete.
This entry describes 3 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 3 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All
Isoform 1 (identifier: Q15417-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MTHFNKGPSY GLSAEVKNKI ASKYDHQAEE DLRNWIEEVT GMSIGPNFQL
60 70 80 90 100
GLKDGIILCE LINKLQPGSV KKVNESSLNW PQLENIGNFI KAIQAYGMKP
110 120 130 140 150
HDIFEANDLF ENGNMTQVQT TLVALAGLAK TKGFHTTIDI GVKYAEKQTR
160 170 180 190 200
RFDEGKLKAG QSVIGLQMGT NKCASQAGMT AYGTRRHLYD PKMQTDKPFD
210 220 230 240 250
QTTISLQMGT NKGASQAGML APGTRRDIYD QKLTLQPVDN STISLQMGTN
260 270 280 290 300
KVASQKGMSV YGLGRQVYDP KYCAAPTEPV IHNGSQGTGT NGSEISDSDY
310 320
QAEYPDEYHG EYQDDYPRDY QYSDQGIDY
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketE9PDU6 | E9PDU6_HUMAN | Calponin | CNN3 | 185 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 21 | A → S in BAG60421 (PubMed:14702039).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_055191 | 1 – 41 | Missing in isoform 2. 1 PublicationAdd BLAST | 41 | |
Alternative sequenceiVSP_055192 | 83 – 128 | Missing in isoform 3. 1 PublicationAdd BLAST | 46 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | S80562 mRNA Translation: AAB35752.1 AK312370 mRNA Translation: BAG35288.1 AK294141 mRNA Translation: BAG57467.1 AK298145 mRNA Translation: BAG60421.1 AK316398 mRNA Translation: BAH14769.1 CR541848 mRNA Translation: CAG46646.1 AC093429 Genomic DNA No translation available. AC105942 Genomic DNA No translation available. AL359554 Genomic DNA Translation: CAC36093.1 CH471097 Genomic DNA Translation: EAW73029.1 BC025372 mRNA Translation: AAH25372.1 |
CCDSi | CCDS30775.1 [Q15417-1] CCDS65592.1 [Q15417-2] CCDS65593.1 [Q15417-3] |
PIRi | JC4501 |
RefSeqi | NP_001272984.1, NM_001286055.1 [Q15417-3] NP_001272985.1, NM_001286056.1 [Q15417-2] NP_001830.1, NM_001839.4 [Q15417-1] XP_016855734.1, XM_017000245.1 [Q15417-2] |
Genome annotation databases
Ensembli | ENST00000370206; ENSP00000359225; ENSG00000117519 [Q15417-1] ENST00000394202; ENSP00000377752; ENSG00000117519 [Q15417-3] ENST00000545882; ENSP00000440081; ENSG00000117519 [Q15417-2] |
GeneIDi | 1266 |
KEGGi | hsa:1266 |
UCSCi | uc001dqz.6, human [Q15417-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | S80562 mRNA Translation: AAB35752.1 AK312370 mRNA Translation: BAG35288.1 AK294141 mRNA Translation: BAG57467.1 AK298145 mRNA Translation: BAG60421.1 AK316398 mRNA Translation: BAH14769.1 CR541848 mRNA Translation: CAG46646.1 AC093429 Genomic DNA No translation available. AC105942 Genomic DNA No translation available. AL359554 Genomic DNA Translation: CAC36093.1 CH471097 Genomic DNA Translation: EAW73029.1 BC025372 mRNA Translation: AAH25372.1 |
CCDSi | CCDS30775.1 [Q15417-1] CCDS65592.1 [Q15417-2] CCDS65593.1 [Q15417-3] |
PIRi | JC4501 |
RefSeqi | NP_001272984.1, NM_001286055.1 [Q15417-3] NP_001272985.1, NM_001286056.1 [Q15417-2] NP_001830.1, NM_001839.4 [Q15417-1] XP_016855734.1, XM_017000245.1 [Q15417-2] |
3D structure databases
SMRi | Q15417 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 107666, 28 interactors |
CORUMi | Q15417 |
IntActi | Q15417, 17 interactors |
MINTi | Q15417 |
STRINGi | 9606.ENSP00000359225 |
PTM databases
GlyConnecti | 1059, 6 N-Linked glycans (1 site) |
GlyGeni | Q15417, 1 site |
iPTMneti | Q15417 |
MetOSitei | Q15417 |
PhosphoSitePlusi | Q15417 |
SwissPalmi | Q15417 |
Genetic variation databases
BioMutai | CNN3 |
DMDMi | 6225157 |
Proteomic databases
EPDi | Q15417 |
jPOSTi | Q15417 |
MassIVEi | Q15417 |
PaxDbi | Q15417 |
PeptideAtlasi | Q15417 |
PRIDEi | Q15417 |
ProteomicsDBi | 30452 4050 60582 [Q15417-1] |
Protocols and materials databases
Antibodypediai | 4078, 216 antibodies |
DNASUi | 1266 |
Genome annotation databases
Ensembli | ENST00000370206; ENSP00000359225; ENSG00000117519 [Q15417-1] ENST00000394202; ENSP00000377752; ENSG00000117519 [Q15417-3] ENST00000545882; ENSP00000440081; ENSG00000117519 [Q15417-2] |
GeneIDi | 1266 |
KEGGi | hsa:1266 |
UCSCi | uc001dqz.6, human [Q15417-1] |
Organism-specific databases
CTDi | 1266 |
DisGeNETi | 1266 |
GeneCardsi | CNN3 |
HGNCi | HGNC:2157, CNN3 |
HPAi | ENSG00000117519, Low tissue specificity |
MIMi | 602374, gene |
neXtProti | NX_Q15417 |
OpenTargetsi | ENSG00000117519 |
PharmGKBi | PA26667 |
VEuPathDBi | HostDB:ENSG00000117519.15 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG2046, Eukaryota |
GeneTreei | ENSGT00940000154539 |
HOGENOMi | CLU_055232_0_2_1 |
InParanoidi | Q15417 |
OMAi | QDDYPRE |
OrthoDBi | 861989at2759 |
PhylomeDBi | Q15417 |
TreeFami | TF313921 |
Enzyme and pathway databases
PathwayCommonsi | Q15417 |
SIGNORi | Q15417 |
Miscellaneous databases
BioGRID-ORCSi | 1266, 6 hits in 989 CRISPR screens |
ChiTaRSi | CNN3, human |
GenomeRNAii | 1266 |
Pharosi | Q15417, Tbio |
PROi | PR:Q15417 |
RNActi | Q15417, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000117519, Expressed in ventricular zone and 243 other tissues |
ExpressionAtlasi | Q15417, baseline and differential |
Genevisiblei | Q15417, HS |
Family and domain databases
CDDi | cd00014, CH, 1 hit |
Gene3Di | 1.10.418.10, 1 hit |
InterProi | View protein in InterPro IPR001997, Calponin/LIMCH1 IPR000557, Calponin_repeat IPR001715, CH-domain IPR036872, CH_dom_sf IPR003096, SM22_calponin |
Pfami | View protein in Pfam PF00402, Calponin, 3 hits PF00307, CH, 1 hit |
PRINTSi | PR00889, CALPONIN PR00888, SM22CALPONIN |
SMARTi | View protein in SMART SM00033, CH, 1 hit |
SUPFAMi | SSF47576, SSF47576, 1 hit |
PROSITEi | View protein in PROSITE PS01052, CALPONIN_1, 3 hits PS51122, CALPONIN_2, 3 hits PS50021, CH, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CNN3_HUMAN | |
Accessioni | Q15417Primary (citable) accession number: Q15417 Secondary accession number(s): B4DFK6 Q6FHA7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 30, 2000 |
Last sequence update: | November 1, 1996 | |
Last modified: | April 7, 2021 | |
This is version 189 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- Human chromosome 1
Human chromosome 1: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families