UniProtKB - Q15398 (DLGP5_HUMAN)
Protein
Disks large-associated protein 5
Gene
DLGAP5
Organism
Homo sapiens (Human)
Status
Functioni
Potential cell cycle regulator that may play a role in carcinogenesis of cancer cells. Mitotic phosphoprotein regulated by the ubiquitin-proteasome pathway. Key regulator of adherens junction integrity and differentiation that may be involved in CDH1-mediated adhesion and signaling in epithelial cells.3 Publications
Caution
It was localized to the spindle and the spindle pole (PubMed:12527899) but was later found to be localized to the spindle and to be excluded from the spindle pole (PubMed:15561729).2 Publications
GO - Molecular functioni
- microtubule binding Source: GO_Central
GO - Biological processi
- centrosome localization Source: GO_Central
- chromosome segregation Source: GO_Central
- kinetochore assembly Source: GO_Central
- mitotic chromosome movement towards spindle pole Source: UniProtKB
- mitotic spindle organization Source: GO_Central
- positive regulation of mitotic metaphase/anaphase transition Source: UniProtKB
- positive regulation of transcription of Notch receptor target Source: Reactome
- regulation of mitotic cell cycle Source: GO_Central
Keywordsi
Biological process | Cell cycle |
Enzyme and pathway databases
PathwayCommonsi | Q15398 |
Reactomei | R-HSA-9013508, NOTCH3 Intracellular Domain Regulates Transcription |
SIGNORi | Q15398 |
Names & Taxonomyi
Protein namesi | Recommended name: Disks large-associated protein 5Short name: DAP-5 Alternative name(s): Discs large homolog 7 Disks large-associated protein DLG7 Hepatoma up-regulated protein Short name: HURP |
Gene namesi | Name:DLGAP5 Synonyms:DLG7, KIAA0008 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:16864, DLGAP5 |
MIMi | 617859, gene |
neXtProti | NX_Q15398 |
VEuPathDBi | HostDB:ENSG00000126787.12 |
Subcellular locationi
Cytoskeleton
- centriolar satellite Source: HPA
- spindle pole centrosome Source: UniProtKB
Cytosol
- cytosol Source: HPA
Mitochondrion
- mitochondrion Source: HPA
Nucleus
- nucleus Source: UniProtKB
Other locations
- cytoplasm Source: GO_Central
Keywords - Cellular componenti
Cytoplasm, Cytoskeleton, NucleusPathology & Biotechi
Organism-specific databases
DisGeNETi | 9787 |
OpenTargetsi | ENSG00000126787 |
PharmGKBi | PA162383761 |
Miscellaneous databases
Pharosi | Q15398, Tbio |
Genetic variation databases
BioMutai | DLGAP5 |
DMDMi | 82592583 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000174299 | 1 – 846 | Disks large-associated protein 5Add BLAST | 846 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 67 | Phosphoserine; by CDK1Combined sources1 Publication | 1 | |
Modified residuei | 202 | PhosphoserineCombined sources | 1 | |
Modified residuei | 326 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 329 | Phosphothreonine; by CDK1Combined sources1 Publication | 1 | |
Modified residuei | 338 | PhosphothreonineCombined sources | 1 | |
Cross-linki | 347 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources | ||
Modified residuei | 401 | Phosphothreonine; by CDK11 Publication | 1 | |
Modified residuei | 402 | Phosphothreonine; by CDK11 Publication | 1 | |
Modified residuei | 618 | Phosphoserine; by CDK1Combined sources1 Publication | 1 | |
Modified residuei | 627 | Phosphoserine; by AURKACombined sources1 Publication | 1 | |
Modified residuei | 629 | PhosphoserineCombined sources | 1 | |
Modified residuei | 634 | PhosphoserineCombined sources | 1 | |
Modified residuei | 639 | Phosphothreonine; by CDK11 Publication | 1 | |
Modified residuei | 642 | Phosphoserine; by CDK11 Publication | 1 | |
Modified residuei | 662 | PhosphoserineCombined sources | 1 | |
Modified residuei | 725 | Phosphoserine; by AURKACombined sources1 Publication | 1 | |
Modified residuei | 757 | Phosphoserine; by AURKA1 Publication | 1 | |
Modified residuei | 759 | Phosphothreonine; by CDK11 Publication | 1 | |
Modified residuei | 774 | PhosphoserineCombined sources | 1 | |
Modified residuei | 777 | PhosphoserineCombined sources | 1 | |
Modified residuei | 784 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 806 | PhosphoserineCombined sources | 1 | |
Modified residuei | 812 | PhosphoserineCombined sources | 1 | |
Modified residuei | 830 | Phosphoserine; by AURKACombined sources1 Publication | 1 | |
Modified residuei | 839 | Phosphoserine; by CDK1Combined sources1 Publication | 1 |
Post-translational modificationi
Ubiquitinated, leading to its degradation.1 Publication
Decreased phosphorylation levels are associated with the differentiation of intestinal epithelial cells.3 Publications
Keywords - PTMi
Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
CPTACi | CPTAC-1204 CPTAC-1205 |
EPDi | Q15398 |
jPOSTi | Q15398 |
MassIVEi | Q15398 |
PaxDbi | Q15398 |
PeptideAtlasi | Q15398 |
PRIDEi | Q15398 |
ProteomicsDBi | 2036 60567 [Q15398-2] 60568 [Q15398-1] |
PTM databases
iPTMneti | Q15398 |
MetOSitei | Q15398 |
PhosphoSitePlusi | Q15398 |
Expressioni
Tissue specificityi
Abundantly expressed in fetal liver. Expressed at lower levels in bone marrow, testis, colon, and placenta.1 Publication
Developmental stagei
Elevated levels of expression detected in the G2/M phase of synchronized cultures of HeLa cells.1 Publication
Gene expression databases
Bgeei | ENSG00000126787, Expressed in secondary oocyte and 144 other tissues |
ExpressionAtlasi | Q15398, baseline and differential |
Genevisiblei | Q15398, HS |
Organism-specific databases
HPAi | ENSG00000126787, Tissue enriched (lymphoid) |
Interactioni
Subunit structurei
Interacts with CDK1.
Interacts with the C-terminal proline-rich region of FBXO7. Recruited by FBXO7 to a SCF (SKP1-CUL1-F-box) protein complex in a CDK1/Cyclin B-phosphorylation dependent manner.
Interacts with CDH1.
2 PublicationsBinary interactionsi
Hide detailsGO - Molecular functioni
- microtubule binding Source: GO_Central
Protein-protein interaction databases
BioGRIDi | 115131, 33 interactors |
ELMi | Q15398 |
IntActi | Q15398, 21 interactors |
MINTi | Q15398 |
STRINGi | 9606.ENSP00000247191 |
Miscellaneous databases
RNActi | Q15398, protein |
Family & Domainsi
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 90 – 120 | Sequence analysisAdd BLAST | 31 |
Sequence similaritiesi
Belongs to the SAPAP family.Curated
Keywords - Domaini
Coiled coilPhylogenomic databases
eggNOGi | KOG3971, Eukaryota |
GeneTreei | ENSGT00940000158652 |
HOGENOMi | CLU_018126_0_0_1 |
InParanoidi | Q15398 |
OMAi | PSERMNL |
OrthoDBi | 566337at2759 |
PhylomeDBi | Q15398 |
TreeFami | TF321382 |
Family and domain databases
InterProi | View protein in InterPro IPR005026, SAPAP |
PANTHERi | PTHR12353, PTHR12353, 1 hit |
Pfami | View protein in Pfam PF03359, GKAP, 1 hit |
s (3+)i Sequence
Sequence statusi: Complete.
This entry describes 3 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 3 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: Q15398-2) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MSSSHFASRH RKDISTEMIR TKIAHRKSLS QKENRHKEYE RNRHFGLKDV
60 70 80 90 100
NIPTLEGRIL VELDETSQGL VPEKTNVKPR AMKTILGDQR KQMLQKYKEE
110 120 130 140 150
KQLQKLKEQR EKAKRGIFKV GRYRPDMPCF LLSNQNAVKA EPKKAIPSSV
160 170 180 190 200
RITRSKAKDQ MEQTKIDNES DVRAIRPGPR QTSEKKVSDK EKKVVQPVMP
210 220 230 240 250
TSLRMTRSAT QAAKQVPRTV SSTTARKPVT RAANENEPEG KVPSKGRPAK
260 270 280 290 300
NVETKPDKGI SCKVDSEENT LNSQTNATSG MNPDGVLSKM ENLPEINTAK
310 320 330 340 350
IKGKNSFAPK DFMFQPLDGL KTYQVTPMTP RSANAFLTPS YTWTPLKTEV
360 370 380 390 400
DESQATKEIL AQKCKTYSTK TIQQDSNKLP CPLGPLTVWH EEHVLNKNEA
410 420 430 440 450
TTKNLNGLPI KEVPSLERNE GRIAQPHHGV PYFRNILQSE TEKLTSHCFE
460 470 480 490 500
WDRKLELDIP DDAKDLIRTA VGQTRLLMKE RFKQFEGLVD DCEYKRGIKE
510 520 530 540 550
TTCTDLDGFW DMVSFQIEDV IHKFNNLIKL EESGWQVNNN MNHNMNKNVF
560 570 580 590 600
RKKVVSGIAS KPKQDDAGRI AARNRLAAIK NAMRERIRQE ECAETAVSVI
610 620 630 640 650
PKEVDKIVFD AGFFRVESPV KLFSGLSVSS EGPSQRLGTP KSVNKAVSQS
660 670 680 690 700
RNEMGIPQQT TSPENAGPQN TKSEHVKKTL FLSIPESRSS IEDAQCPGLP
710 720 730 740 750
DLIEENHVVN KTDLKVDCLS SERMSLPLLA GGVADDINTN KKEGISDVVE
760 770 780 790 800
GMELNSSITS QDVLMSSPEK NTASQNSILE EGETKISQSE LFDNKSLTTE
810 820 830 840
CHLLDSPGLN CSNPFTQLER RHQEHARHIS FGGNLITFSP LQPGEF
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketG3V4E5 | G3V4E5_HUMAN | Disks large-associated protein 5 | DLGAP5 | 234 | Annotation score: | ||
G3V543 | G3V543_HUMAN | Disks large-associated protein 5 | DLGAP5 | 111 | Annotation score: |
Sequence cautioni
The sequence BAA02797 differs from that shown. Reason: Erroneous initiation. Extended N-terminus.Curated
The sequence CAD62583 differs from that shown. Reason: Erroneous initiation. Extended N-terminus.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 253 | E → K in BAA02797 (PubMed:7584026).Curated | 1 | |
Sequence conflicti | 328 | M → T in BAG61340 (PubMed:14702039).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_023774 | 69 | G → E1 PublicationCorresponds to variant dbSNP:rs2274271Ensembl. | 1 | |
Natural variantiVAR_057718 | 324 | Q → H. Corresponds to variant dbSNP:rs8010791Ensembl. | 1 | |
Natural variantiVAR_057719 | 469 | T → I. Corresponds to variant dbSNP:rs17128275Ensembl. | 1 | |
Natural variantiVAR_062147 | 753 | E → Q. Corresponds to variant dbSNP:rs35954941Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_015550 | 1 – 81 | Missing in isoform 2. 1 PublicationAdd BLAST | 81 | |
Alternative sequenceiVSP_045341 | 807 – 846 | PGLNC…QPGEF → VGSCYVARAGLEVLGSSDPT TSASRVAGTTARSKLQ in isoform 3. 1 PublicationAdd BLAST | 40 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB076695 mRNA Translation: BAB97376.1 D13633 mRNA Translation: BAA02797.3 Different initiation. BX248255 mRNA Translation: CAD62583.1 Different initiation. BT007344 mRNA Translation: AAP36008.1 AK299338 mRNA Translation: BAG61340.1 AL139316 Genomic DNA No translation available. CH471061 Genomic DNA Translation: EAW80664.1 BC010658 mRNA Translation: AAH10658.2 BC016276 mRNA Translation: AAH16276.2 |
CCDSi | CCDS53897.1 [Q15398-3] CCDS9723.1 [Q15398-2] |
RefSeqi | NP_001139487.1, NM_001146015.1 [Q15398-3] NP_055565.3, NM_014750.4 [Q15398-2] XP_016877329.1, XM_017021840.1 [Q15398-2] |
Genome annotation databases
Ensembli | ENST00000247191; ENSP00000247191; ENSG00000126787 [Q15398-2] ENST00000395425; ENSP00000378815; ENSG00000126787 [Q15398-3] |
GeneIDi | 9787 |
KEGGi | hsa:9787 |
UCSCi | uc001xbs.4, human [Q15398-2] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB076695 mRNA Translation: BAB97376.1 D13633 mRNA Translation: BAA02797.3 Different initiation. BX248255 mRNA Translation: CAD62583.1 Different initiation. BT007344 mRNA Translation: AAP36008.1 AK299338 mRNA Translation: BAG61340.1 AL139316 Genomic DNA No translation available. CH471061 Genomic DNA Translation: EAW80664.1 BC010658 mRNA Translation: AAH10658.2 BC016276 mRNA Translation: AAH16276.2 |
CCDSi | CCDS53897.1 [Q15398-3] CCDS9723.1 [Q15398-2] |
RefSeqi | NP_001139487.1, NM_001146015.1 [Q15398-3] NP_055565.3, NM_014750.4 [Q15398-2] XP_016877329.1, XM_017021840.1 [Q15398-2] |
3D structure databases
SMRi | Q15398 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 115131, 33 interactors |
ELMi | Q15398 |
IntActi | Q15398, 21 interactors |
MINTi | Q15398 |
STRINGi | 9606.ENSP00000247191 |
PTM databases
iPTMneti | Q15398 |
MetOSitei | Q15398 |
PhosphoSitePlusi | Q15398 |
Genetic variation databases
BioMutai | DLGAP5 |
DMDMi | 82592583 |
Proteomic databases
CPTACi | CPTAC-1204 CPTAC-1205 |
EPDi | Q15398 |
jPOSTi | Q15398 |
MassIVEi | Q15398 |
PaxDbi | Q15398 |
PeptideAtlasi | Q15398 |
PRIDEi | Q15398 |
ProteomicsDBi | 2036 60567 [Q15398-2] 60568 [Q15398-1] |
Protocols and materials databases
ABCDi | Q15398, 3 sequenced antibodies |
Antibodypediai | 5, 245 antibodies |
DNASUi | 9787 |
Genome annotation databases
Ensembli | ENST00000247191; ENSP00000247191; ENSG00000126787 [Q15398-2] ENST00000395425; ENSP00000378815; ENSG00000126787 [Q15398-3] |
GeneIDi | 9787 |
KEGGi | hsa:9787 |
UCSCi | uc001xbs.4, human [Q15398-2] |
Organism-specific databases
CTDi | 9787 |
DisGeNETi | 9787 |
GeneCardsi | DLGAP5 |
HGNCi | HGNC:16864, DLGAP5 |
HPAi | ENSG00000126787, Tissue enriched (lymphoid) |
MIMi | 617859, gene |
neXtProti | NX_Q15398 |
OpenTargetsi | ENSG00000126787 |
PharmGKBi | PA162383761 |
VEuPathDBi | HostDB:ENSG00000126787.12 |
HUGEi | Search... |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG3971, Eukaryota |
GeneTreei | ENSGT00940000158652 |
HOGENOMi | CLU_018126_0_0_1 |
InParanoidi | Q15398 |
OMAi | PSERMNL |
OrthoDBi | 566337at2759 |
PhylomeDBi | Q15398 |
TreeFami | TF321382 |
Enzyme and pathway databases
PathwayCommonsi | Q15398 |
Reactomei | R-HSA-9013508, NOTCH3 Intracellular Domain Regulates Transcription |
SIGNORi | Q15398 |
Miscellaneous databases
BioGRID-ORCSi | 9787, 48 hits in 1010 CRISPR screens |
ChiTaRSi | DLGAP5, human |
GeneWikii | DLGAP5 |
GenomeRNAii | 9787 |
Pharosi | Q15398, Tbio |
PROi | PR:Q15398 |
RNActi | Q15398, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000126787, Expressed in secondary oocyte and 144 other tissues |
ExpressionAtlasi | Q15398, baseline and differential |
Genevisiblei | Q15398, HS |
Family and domain databases
InterProi | View protein in InterPro IPR005026, SAPAP |
PANTHERi | PTHR12353, PTHR12353, 1 hit |
Pfami | View protein in Pfam PF03359, GKAP, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | DLGP5_HUMAN | |
Accessioni | Q15398Primary (citable) accession number: Q15398 Secondary accession number(s): A8MTM6 Q8NG58 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 15, 1998 |
Last sequence update: | September 13, 2005 | |
Last modified: | April 7, 2021 | |
This is version 175 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human chromosome 14
Human chromosome 14: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families