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Protein

Disks large-associated protein 5

Gene

DLGAP5

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Potential cell cycle regulator that may play a role in carcinogenesis of cancer cells. Mitotic phosphoprotein regulated by the ubiquitin-proteasome pathway. Key regulator of adherens junction integrity and differentiation that may be involved in CDH1-mediated adhesion and signaling in epithelial cells.3 Publications

Caution

It was localized to the spindle and the spindle pole (PubMed:12527899) but was later found to be localized to the spindle and to be excluded from the spindle pole (PubMed:15561729).2 Publications

GO - Molecular functioni

  • phosphoprotein phosphatase activity Source: UniProtKB

GO - Biological processi

Keywordsi

Biological processCell cycle

Enzyme and pathway databases

ReactomeiR-HSA-9013508 NOTCH3 Intracellular Domain Regulates Transcription

Names & Taxonomyi

Protein namesi
Recommended name:
Disks large-associated protein 5
Short name:
DAP-5
Alternative name(s):
Discs large homolog 7
Disks large-associated protein DLG7
Hepatoma up-regulated protein
Short name:
HURP
Gene namesi
Name:DLGAP5
Synonyms:DLG7, KIAA0008
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 14

Organism-specific databases

EuPathDBiHostDB:ENSG00000126787.12
HGNCiHGNC:16864 DLGAP5
MIMi617859 gene
neXtProtiNX_Q15398

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi9787
OpenTargetsiENSG00000126787
PharmGKBiPA162383761

Polymorphism and mutation databases

BioMutaiDLGAP5
DMDMi82592583

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001742991 – 846Disks large-associated protein 5Add BLAST846

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei67Phosphoserine; by CDK1Combined sources1 Publication1
Modified residuei202PhosphoserineCombined sources1
Modified residuei326PhosphothreonineCombined sources1
Modified residuei329Phosphothreonine; by CDK1Combined sources1 Publication1
Modified residuei338PhosphothreonineCombined sources1
Cross-linki347Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei401Phosphothreonine; by CDK11 Publication1
Modified residuei402Phosphothreonine; by CDK11 Publication1
Modified residuei618Phosphoserine; by CDK1Combined sources1 Publication1
Modified residuei627Phosphoserine; by AURKACombined sources1 Publication1
Modified residuei629PhosphoserineCombined sources1
Modified residuei634PhosphoserineCombined sources1
Modified residuei639Phosphothreonine; by CDK11 Publication1
Modified residuei642Phosphoserine; by CDK11 Publication1
Modified residuei662PhosphoserineCombined sources1
Modified residuei725Phosphoserine; by AURKACombined sources1 Publication1
Modified residuei757Phosphoserine; by AURKA1 Publication1
Modified residuei759Phosphothreonine; by CDK11 Publication1
Modified residuei774PhosphoserineCombined sources1
Modified residuei777PhosphoserineCombined sources1
Modified residuei784PhosphothreonineCombined sources1
Modified residuei806PhosphoserineCombined sources1
Modified residuei812PhosphoserineCombined sources1
Modified residuei830Phosphoserine; by AURKACombined sources1 Publication1
Modified residuei839Phosphoserine; by CDK1Combined sources1 Publication1

Post-translational modificationi

Ubiquitinated, leading to its degradation.1 Publication
Decreased phosphorylation levels are associated with the differentiation of intestinal epithelial cells.3 Publications

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ15398
PaxDbiQ15398
PeptideAtlasiQ15398
PRIDEiQ15398
ProteomicsDBi60567
60568 [Q15398-1]

PTM databases

iPTMnetiQ15398
PhosphoSitePlusiQ15398

Expressioni

Tissue specificityi

Abundantly expressed in fetal liver. Expressed at lower levels in bone marrow, testis, colon, and placenta.1 Publication

Developmental stagei

Elevated levels of expression detected in the G2/M phase of synchronized cultures of HeLa cells.1 Publication

Gene expression databases

BgeeiENSG00000126787 Expressed in 126 organ(s), highest expression level in secondary oocyte
CleanExiHS_DLGAP5
ExpressionAtlasiQ15398 baseline and differential
GenevisibleiQ15398 HS

Organism-specific databases

HPAiHPA005546

Interactioni

Subunit structurei

Interacts with CDK1. Interacts with the C-terminal proline-rich region of FBXO7. Recruited by FBXO7 to a SCF (SKP1-CUL1-F-box) protein complex in a CDK1/Cyclin B-phosphorylation dependent manner. Interacts with CDH1.2 Publications

Binary interactionsi

Protein-protein interaction databases

BioGridi115131, 22 interactors
ELMiQ15398
IntActiQ15398, 9 interactors
MINTiQ15398
STRINGi9606.ENSP00000247191

Structurei

3D structure databases

ProteinModelPortaliQ15398
SMRiQ15398
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili90 – 120Sequence analysisAdd BLAST31

Sequence similaritiesi

Belongs to the SAPAP family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG3971 Eukaryota
ENOG4111JKV LUCA
GeneTreeiENSGT00550000074473
HOGENOMiHOG000054220
HOVERGENiHBG062172
InParanoidiQ15398
KOiK16804
OMAiKLQCPLG
OrthoDBiEOG091G0224
PhylomeDBiQ15398
TreeFamiTF321382

Family and domain databases

InterProiView protein in InterPro
IPR005026 SAPAP
PANTHERiPTHR12353 PTHR12353, 1 hit
PfamiView protein in Pfam
PF03359 GKAP, 1 hit

Sequences (3+)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 3 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q15398-2) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MSSSHFASRH RKDISTEMIR TKIAHRKSLS QKENRHKEYE RNRHFGLKDV
60 70 80 90 100
NIPTLEGRIL VELDETSQGL VPEKTNVKPR AMKTILGDQR KQMLQKYKEE
110 120 130 140 150
KQLQKLKEQR EKAKRGIFKV GRYRPDMPCF LLSNQNAVKA EPKKAIPSSV
160 170 180 190 200
RITRSKAKDQ MEQTKIDNES DVRAIRPGPR QTSEKKVSDK EKKVVQPVMP
210 220 230 240 250
TSLRMTRSAT QAAKQVPRTV SSTTARKPVT RAANENEPEG KVPSKGRPAK
260 270 280 290 300
NVETKPDKGI SCKVDSEENT LNSQTNATSG MNPDGVLSKM ENLPEINTAK
310 320 330 340 350
IKGKNSFAPK DFMFQPLDGL KTYQVTPMTP RSANAFLTPS YTWTPLKTEV
360 370 380 390 400
DESQATKEIL AQKCKTYSTK TIQQDSNKLP CPLGPLTVWH EEHVLNKNEA
410 420 430 440 450
TTKNLNGLPI KEVPSLERNE GRIAQPHHGV PYFRNILQSE TEKLTSHCFE
460 470 480 490 500
WDRKLELDIP DDAKDLIRTA VGQTRLLMKE RFKQFEGLVD DCEYKRGIKE
510 520 530 540 550
TTCTDLDGFW DMVSFQIEDV IHKFNNLIKL EESGWQVNNN MNHNMNKNVF
560 570 580 590 600
RKKVVSGIAS KPKQDDAGRI AARNRLAAIK NAMRERIRQE ECAETAVSVI
610 620 630 640 650
PKEVDKIVFD AGFFRVESPV KLFSGLSVSS EGPSQRLGTP KSVNKAVSQS
660 670 680 690 700
RNEMGIPQQT TSPENAGPQN TKSEHVKKTL FLSIPESRSS IEDAQCPGLP
710 720 730 740 750
DLIEENHVVN KTDLKVDCLS SERMSLPLLA GGVADDINTN KKEGISDVVE
760 770 780 790 800
GMELNSSITS QDVLMSSPEK NTASQNSILE EGETKISQSE LFDNKSLTTE
810 820 830 840
CHLLDSPGLN CSNPFTQLER RHQEHARHIS FGGNLITFSP LQPGEF
Length:846
Mass (Da):95,115
Last modified:September 13, 2005 - v2
Checksum:i588BAF238D6FFB72
GO
Isoform 2 (identifier: Q15398-1) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-81: Missing.

Note: No experimental confirmation available.
Show »
Length:765
Mass (Da):85,668
Checksum:i00AFF91A02387EA1
GO
Isoform 3 (identifier: Q15398-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     807-846: PGLNCSNPFT...TFSPLQPGEF → VGSCYVARAG...AGTTARSKLQ

Note: No experimental confirmation available.
Show »
Length:842
Mass (Da):94,178
Checksum:iFDEF1E4B900F758C
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
G3V4E5G3V4E5_HUMAN
Disks large-associated protein 5
DLGAP5
234Annotation score:
G3V543G3V543_HUMAN
Disks large-associated protein 5
DLGAP5
111Annotation score:

Sequence cautioni

The sequence BAA02797 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence CAD62583 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti253E → K in BAA02797 (PubMed:7584026).Curated1
Sequence conflicti328M → T in BAG61340 (PubMed:14702039).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_02377469G → E1 PublicationCorresponds to variant dbSNP:rs2274271Ensembl.1
Natural variantiVAR_057718324Q → H. Corresponds to variant dbSNP:rs8010791Ensembl.1
Natural variantiVAR_057719469T → I. Corresponds to variant dbSNP:rs17128275Ensembl.1
Natural variantiVAR_062147753E → Q. Corresponds to variant dbSNP:rs35954941Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0155501 – 81Missing in isoform 2. 1 PublicationAdd BLAST81
Alternative sequenceiVSP_045341807 – 846PGLNC…QPGEF → VGSCYVARAGLEVLGSSDPT TSASRVAGTTARSKLQ in isoform 3. 1 PublicationAdd BLAST40

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB076695 mRNA Translation: BAB97376.1
D13633 mRNA Translation: BAA02797.3 Different initiation.
BX248255 mRNA Translation: CAD62583.1 Different initiation.
BT007344 mRNA Translation: AAP36008.1
AK299338 mRNA Translation: BAG61340.1
AL139316 Genomic DNA No translation available.
CH471061 Genomic DNA Translation: EAW80664.1
BC010658 mRNA Translation: AAH10658.2
BC016276 mRNA Translation: AAH16276.2
CCDSiCCDS53897.1 [Q15398-3]
CCDS9723.1 [Q15398-2]
RefSeqiNP_001139487.1, NM_001146015.1 [Q15398-3]
NP_055565.3, NM_014750.4 [Q15398-2]
XP_016877329.1, XM_017021840.1 [Q15398-2]
UniGeneiHs.77695

Genome annotation databases

EnsembliENST00000247191; ENSP00000247191; ENSG00000126787 [Q15398-2]
ENST00000395425; ENSP00000378815; ENSG00000126787 [Q15398-3]
GeneIDi9787
KEGGihsa:9787
UCSCiuc001xbs.4 human [Q15398-2]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB076695 mRNA Translation: BAB97376.1
D13633 mRNA Translation: BAA02797.3 Different initiation.
BX248255 mRNA Translation: CAD62583.1 Different initiation.
BT007344 mRNA Translation: AAP36008.1
AK299338 mRNA Translation: BAG61340.1
AL139316 Genomic DNA No translation available.
CH471061 Genomic DNA Translation: EAW80664.1
BC010658 mRNA Translation: AAH10658.2
BC016276 mRNA Translation: AAH16276.2
CCDSiCCDS53897.1 [Q15398-3]
CCDS9723.1 [Q15398-2]
RefSeqiNP_001139487.1, NM_001146015.1 [Q15398-3]
NP_055565.3, NM_014750.4 [Q15398-2]
XP_016877329.1, XM_017021840.1 [Q15398-2]
UniGeneiHs.77695

3D structure databases

ProteinModelPortaliQ15398
SMRiQ15398
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi115131, 22 interactors
ELMiQ15398
IntActiQ15398, 9 interactors
MINTiQ15398
STRINGi9606.ENSP00000247191

PTM databases

iPTMnetiQ15398
PhosphoSitePlusiQ15398

Polymorphism and mutation databases

BioMutaiDLGAP5
DMDMi82592583

Proteomic databases

EPDiQ15398
PaxDbiQ15398
PeptideAtlasiQ15398
PRIDEiQ15398
ProteomicsDBi60567
60568 [Q15398-1]

Protocols and materials databases

DNASUi9787
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000247191; ENSP00000247191; ENSG00000126787 [Q15398-2]
ENST00000395425; ENSP00000378815; ENSG00000126787 [Q15398-3]
GeneIDi9787
KEGGihsa:9787
UCSCiuc001xbs.4 human [Q15398-2]

Organism-specific databases

CTDi9787
DisGeNETi9787
EuPathDBiHostDB:ENSG00000126787.12
GeneCardsiDLGAP5
H-InvDBiHIX0011684
HGNCiHGNC:16864 DLGAP5
HPAiHPA005546
MIMi617859 gene
neXtProtiNX_Q15398
OpenTargetsiENSG00000126787
PharmGKBiPA162383761
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3971 Eukaryota
ENOG4111JKV LUCA
GeneTreeiENSGT00550000074473
HOGENOMiHOG000054220
HOVERGENiHBG062172
InParanoidiQ15398
KOiK16804
OMAiKLQCPLG
OrthoDBiEOG091G0224
PhylomeDBiQ15398
TreeFamiTF321382

Enzyme and pathway databases

ReactomeiR-HSA-9013508 NOTCH3 Intracellular Domain Regulates Transcription

Miscellaneous databases

ChiTaRSiDLGAP5 human
GeneWikiiDLGAP5
GenomeRNAii9787
PROiPR:Q15398
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000126787 Expressed in 126 organ(s), highest expression level in secondary oocyte
CleanExiHS_DLGAP5
ExpressionAtlasiQ15398 baseline and differential
GenevisibleiQ15398 HS

Family and domain databases

InterProiView protein in InterPro
IPR005026 SAPAP
PANTHERiPTHR12353 PTHR12353, 1 hit
PfamiView protein in Pfam
PF03359 GKAP, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiDLGP5_HUMAN
AccessioniPrimary (citable) accession number: Q15398
Secondary accession number(s): A8MTM6
, B4DRM8, Q86T11, Q8NG58
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: September 13, 2005
Last modified: November 7, 2018
This is version 158 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. Human chromosome 14
    Human chromosome 14: entries, gene names and cross-references to MIM
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Main funding by: National Institutes of Health

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