UniProtKB - Q15386 (UBE3C_HUMAN)
Protein
Ubiquitin-protein ligase E3C
Gene
UBE3C
Organism
Homo sapiens (Human)
Status
Functioni
E3 ubiquitin-protein ligase that accepts ubiquitin from the E2 ubiquitin-conjugating enzyme UBE2D1 in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Can assemble unanchored poly-ubiquitin chains in either 'Lys-29'- or 'Lys-48'-linked polyubiquitin chains. Has preference for 'Lys-48' linkages. It can target itself for ubiquitination in vitro and may promote its own degradation in vivo.4 Publications
Miscellaneous
A cysteine residue is required for ubiquitin-thioester formation.
Catalytic activityi
- S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine. EC:2.3.2.26
: protein ubiquitination Pathwayi
This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 1051 | Glycyl thioester intermediate | 1 |
GO - Molecular functioni
- ubiquitin protein ligase activity Source: UniProtKB
GO - Biological processi
- protein polyubiquitination Source: UniProtKB
- ubiquitin-dependent protein catabolic process Source: GO_Central
Keywordsi
Molecular function | Transferase |
Biological process | Ubl conjugation pathway |
Enzyme and pathway databases
PathwayCommonsi | Q15386 |
Reactomei | R-HSA-983168, Antigen processing: Ubiquitination & Proteasome degradation |
UniPathwayi | UPA00143 |
Names & Taxonomyi
Protein namesi | Recommended name: Ubiquitin-protein ligase E3C (EC:2.3.2.26)Alternative name(s): HECT-type ubiquitin transferase E3C HectH2 |
Gene namesi | Name:UBE3C Synonyms:KIAA0010, KIAA10 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:16803, UBE3C |
MIMi | 614454, gene |
neXtProti | NX_Q15386 |
VEuPathDBi | HostDB:ENSG00000009335.17 |
Subcellular locationi
Nucleus
- Nucleus Curated
Nucleus
- nucleus Source: UniProtKB-SubCell
Other locations
- proteasome complex Source: UniProtKB-KW
Keywords - Cellular componenti
Nucleus, ProteasomePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 1051 | C → A: No stimulation of in vitro CAND2 ubiquitination. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 9690 |
OpenTargetsi | ENSG00000009335 |
PharmGKBi | PA134905339 |
Miscellaneous databases
Pharosi | Q15386, Tbio |
Genetic variation databases
BioMutai | UBE3C |
DMDMi | 67462009 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000194982 | 1 – 1083 | Ubiquitin-protein ligase E3CAdd BLAST | 1083 |
Proteomic databases
EPDi | Q15386 |
jPOSTi | Q15386 |
MassIVEi | Q15386 |
MaxQBi | Q15386 |
PaxDbi | Q15386 |
PeptideAtlasi | Q15386 |
PRIDEi | Q15386 |
ProteomicsDBi | 60555 [Q15386-1] 60556 [Q15386-2] 60557 [Q15386-3] |
PTM databases
iPTMneti | Q15386 |
PhosphoSitePlusi | Q15386 |
Expressioni
Tissue specificityi
Highly expressed in skeletal muscle. Detected at much lower levels in kidney and pancreas.3 Publications
Gene expression databases
Bgeei | ENSG00000009335, Expressed in sural nerve and 249 other tissues |
ExpressionAtlasi | Q15386, baseline and differential |
Genevisiblei | Q15386, HS |
Organism-specific databases
HPAi | ENSG00000009335, Low tissue specificity |
Interactioni
Subunit structurei
Interacts with 26S proteasomes.
Interacts (via the N-terminal) with CAND2; the interaction stimulates ubiquitination of CAND2 in vitro. May interact (via the HECT domain) with UBE2D1 and, less efficiently, with UBE2L3.
3 PublicationsBinary interactionsi
Hide detailsQ15386
With | #Exp. | IntAct |
---|---|---|
GOLGA2 [Q08379] | 3 | EBI-1058871,EBI-618309 |
Isoform 3 [Q15386-3]
With | #Exp. | IntAct |
---|---|---|
BDNF - isoform 2 [P23560-2] | 3 | EBI-25833079,EBI-12275524 |
Protein-protein interaction databases
BioGRIDi | 115043, 110 interactors |
IntActi | Q15386, 24 interactors |
MINTi | Q15386 |
STRINGi | 9606.ENSP00000309198 |
Miscellaneous databases
RNActi | Q15386, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q15386 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 45 – 74 | IQPROSITE-ProRule annotationAdd BLAST | 30 | |
Domaini | 744 – 1083 | HECTPROSITE-ProRule annotationAdd BLAST | 340 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 60 | Cis-determinant of acceptor ubiquitin-bindingAdd BLAST | 60 |
Domaini
The C-terminal is necessary and sufficient for the poly-ubiquitin chain assembly.
Phylogenomic databases
eggNOGi | KOG0942, Eukaryota |
GeneTreei | ENSGT00940000156321 |
HOGENOMi | CLU_002173_2_1_1 |
InParanoidi | Q15386 |
OMAi | KCPPLDG |
OrthoDBi | 1163565at2759 |
PhylomeDBi | Q15386 |
TreeFami | TF106144 |
Family and domain databases
CDDi | cd00078, HECTc, 1 hit |
InterProi | View protein in InterPro IPR000569, HECT_dom IPR035983, Hect_E3_ubiquitin_ligase IPR000048, IQ_motif_EF-hand-BS |
Pfami | View protein in Pfam PF00632, HECT, 1 hit |
SMARTi | View protein in SMART SM00119, HECTc, 1 hit SM00015, IQ, 1 hit |
SUPFAMi | SSF56204, SSF56204, 1 hit |
PROSITEi | View protein in PROSITE PS50237, HECT, 1 hit PS50096, IQ, 1 hit |
s (3+)i Sequence
Sequence statusi: Complete.
This entry describes 3 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 3 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All
Isoform 1 (identifier: Q15386-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MFSFEGDFKT RPKVSLGGAS RKEEKASLLH RTQEERRKRE EERRRLKNAI
60 70 80 90 100
IIQSFIRGYR DRKQQYSIQR SAFDRCATLS QSGGAFPIAN GPNLTLLVRQ
110 120 130 140 150
LLFFYKQNED SKRLIWLYQN LIKHSSLFVK QLDGSERLTC LFQIKRLMSL
160 170 180 190 200
CCRLLQNCND DSLNVALPMR MLEVFSSENT YLPVLQDASY VVSVIEQILH
210 220 230 240 250
YMIHNGYYRS LYLLINSKLP SSIEYSDLSR VPIAKILLEN VLKPLHFTYN
260 270 280 290 300
SCPEGARQQV FTAFTEEFLA APFTDQIFHF IIPALADAQT VFPYEPFLNA
310 320 330 340 350
LLLIESRCSR KSGGAPWLFY FVLTVGENYL GALSEEGLLV YLRVLQTFLS
360 370 380 390 400
QLPVSPASAS CHDSASDSEE ESEEADKPSS PEDGRLSVSY ITEECLKKLD
410 420 430 440 450
TKQQTNTLLN LVWRDSASEE VFTTMASVCH TLMVQHRMMV PKVRLLYSLA
460 470 480 490 500
FNARFLRHLW FLISSMSTRM ITGSMVPLLQ VISRGSPMSF EDSSRIIPLF
510 520 530 540 550
YLFSSLFSHS LISIHDNEFF GDPIEVVGQR QSSMMPFTLE ELIMLSRCLR
560 570 580 590 600
DACLGIIKLA YPETKPEVRE EYITAFQSIG VTTSSEMQQC IQMEQKRWIQ
610 620 630 640 650
LFKVITNLVK MLKSRDTRRN FCPPNHWLSE QEDIKADKVT QLYVPASRHV
660 670 680 690 700
WRFRRMGRIG PLQSTLDVGL ESPPLSVSEE RQLAVLTELP FVVPFEERVK
710 720 730 740 750
IFQRLIYADK QEVQGDGPFL DGINVTIRRN YIYEDAYDKL SPENEPDLKK
760 770 780 790 800
RIRVHLLNAH GLDEAGIDGG GIFREFLNEL LKSGFNPNQG FFKTTNEGLL
810 820 830 840 850
YPNPAAQMLV GDSFARHYYF LGRMLGKALY ENMLVELPFA GFFLSKLLGT
860 870 880 890 900
SADVDIHHLA SLDPEVYKNL LFLKSYEDDV EELGLNFTVV NNDLGEAQVV
910 920 930 940 950
ELKFGGKDIP VTSANRIAYI HLVADYRLNR QIRQHCLAFR QGLANVVSLE
960 970 980 990 1000
WLRMFDQQEI QVLISGAQVP ISLEDLKSFT NYSGGYSADH PVIKVFWRVV
1010 1020 1030 1040 1050
EGFTDEEKRK LLKFVTSCSR PPLLGFKELY PAFCIHNGGS DLERLPTAST
1060 1070 1080
CMNLLKLPEF YDETLLRSKL LYAIECAAGF ELS
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketH7C0Y1 | H7C0Y1_HUMAN | Ubiquitin-protein ligase E3C | UBE3C | 56 | Annotation score: |
Sequence cautioni
The sequence BAA02799 differs from that shown. Reason: Erroneous initiation. Extended N-terminus.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 822 | G → A in BAA02799 (PubMed:7584026).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_013953 | 23 – 65 | Missing in isoform 3. 1 PublicationAdd BLAST | 43 | |
Alternative sequenceiVSP_013954 | 445 – 447 | LLY → VYK in isoform 3. 1 Publication | 3 | |
Alternative sequenceiVSP_013955 | 448 – 1083 | Missing in isoform 3. 1 PublicationAdd BLAST | 636 | |
Alternative sequenceiVSP_013956 | 640 – 649 | TQLYVPASRH → LLKDLFNIYH in isoform 2. 1 Publication | 10 | |
Alternative sequenceiVSP_013957 | 650 – 1083 | Missing in isoform 2. 1 PublicationAdd BLAST | 434 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D13635 mRNA Translation: BAA02799.2 Different initiation. AC004898 Genomic DNA No translation available. AC004975 Genomic DNA Translation: AAD51453.1 CH236954 Genomic DNA Translation: EAL23922.1 CH471149 Genomic DNA Translation: EAX04568.1 BC014029 mRNA Translation: AAH14029.1 BC026241 mRNA Translation: AAH26241.1 |
CCDSi | CCDS34789.1 [Q15386-1] |
PIRi | A38919 |
RefSeqi | NP_055486.2, NM_014671.2 [Q15386-1] |
Genome annotation databases
Ensembli | ENST00000348165; ENSP00000309198; ENSG00000009335 [Q15386-1] ENST00000389103; ENSP00000373755; ENSG00000009335 [Q15386-3] ENST00000611269; ENSP00000481584; ENSG00000009335 [Q15386-3] |
GeneIDi | 9690 |
KEGGi | hsa:9690 |
UCSCi | uc003wnf.3, human [Q15386-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D13635 mRNA Translation: BAA02799.2 Different initiation. AC004898 Genomic DNA No translation available. AC004975 Genomic DNA Translation: AAD51453.1 CH236954 Genomic DNA Translation: EAL23922.1 CH471149 Genomic DNA Translation: EAX04568.1 BC014029 mRNA Translation: AAH14029.1 BC026241 mRNA Translation: AAH26241.1 |
CCDSi | CCDS34789.1 [Q15386-1] |
PIRi | A38919 |
RefSeqi | NP_055486.2, NM_014671.2 [Q15386-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
6K2C | X-ray | 2.70 | A | 693-1083 | [»] | |
SMRi | Q15386 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 115043, 110 interactors |
IntActi | Q15386, 24 interactors |
MINTi | Q15386 |
STRINGi | 9606.ENSP00000309198 |
PTM databases
iPTMneti | Q15386 |
PhosphoSitePlusi | Q15386 |
Genetic variation databases
BioMutai | UBE3C |
DMDMi | 67462009 |
Proteomic databases
EPDi | Q15386 |
jPOSTi | Q15386 |
MassIVEi | Q15386 |
MaxQBi | Q15386 |
PaxDbi | Q15386 |
PeptideAtlasi | Q15386 |
PRIDEi | Q15386 |
ProteomicsDBi | 60555 [Q15386-1] 60556 [Q15386-2] 60557 [Q15386-3] |
Protocols and materials databases
Antibodypediai | 33178, 150 antibodies |
DNASUi | 9690 |
Genome annotation databases
Ensembli | ENST00000348165; ENSP00000309198; ENSG00000009335 [Q15386-1] ENST00000389103; ENSP00000373755; ENSG00000009335 [Q15386-3] ENST00000611269; ENSP00000481584; ENSG00000009335 [Q15386-3] |
GeneIDi | 9690 |
KEGGi | hsa:9690 |
UCSCi | uc003wnf.3, human [Q15386-1] |
Organism-specific databases
CTDi | 9690 |
DisGeNETi | 9690 |
GeneCardsi | UBE3C |
HGNCi | HGNC:16803, UBE3C |
HPAi | ENSG00000009335, Low tissue specificity |
MIMi | 614454, gene |
neXtProti | NX_Q15386 |
OpenTargetsi | ENSG00000009335 |
PharmGKBi | PA134905339 |
VEuPathDBi | HostDB:ENSG00000009335.17 |
HUGEi | Search... Search... |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG0942, Eukaryota |
GeneTreei | ENSGT00940000156321 |
HOGENOMi | CLU_002173_2_1_1 |
InParanoidi | Q15386 |
OMAi | KCPPLDG |
OrthoDBi | 1163565at2759 |
PhylomeDBi | Q15386 |
TreeFami | TF106144 |
Enzyme and pathway databases
UniPathwayi | UPA00143 |
PathwayCommonsi | Q15386 |
Reactomei | R-HSA-983168, Antigen processing: Ubiquitination & Proteasome degradation |
Miscellaneous databases
BioGRID-ORCSi | 9690, 18 hits in 1007 CRISPR screens |
ChiTaRSi | UBE3C, human |
GeneWikii | UBE3C |
GenomeRNAii | 9690 |
Pharosi | Q15386, Tbio |
PROi | PR:Q15386 |
RNActi | Q15386, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000009335, Expressed in sural nerve and 249 other tissues |
ExpressionAtlasi | Q15386, baseline and differential |
Genevisiblei | Q15386, HS |
Family and domain databases
CDDi | cd00078, HECTc, 1 hit |
InterProi | View protein in InterPro IPR000569, HECT_dom IPR035983, Hect_E3_ubiquitin_ligase IPR000048, IQ_motif_EF-hand-BS |
Pfami | View protein in Pfam PF00632, HECT, 1 hit |
SMARTi | View protein in SMART SM00119, HECTc, 1 hit SM00015, IQ, 1 hit |
SUPFAMi | SSF56204, SSF56204, 1 hit |
PROSITEi | View protein in PROSITE PS50237, HECT, 1 hit PS50096, IQ, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | UBE3C_HUMAN | |
Accessioni | Q15386Primary (citable) accession number: Q15386 Secondary accession number(s): A4D235 Q9UDU3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 7, 2005 |
Last sequence update: | June 7, 2005 | |
Last modified: | April 7, 2021 | |
This is version 176 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- Human chromosome 7
Human chromosome 7: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references