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Protein

Poly(rC)-binding protein 2

Gene

PCBP2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Single-stranded nucleic acid binding protein that binds preferentially to oligo dC. Major cellular poly(rC)-binding protein. Binds also poly(rU). Negatively regulates cellular antiviral responses mediated by MAVS signaling (PubMed:19881509). It acts as an adapter between MAVS and the E3 ubiquitin ligase ITCH, therefore triggering MAVS ubiquitination and degradation (PubMed:19881509).2 Publications
(Microbial infection) In case of infection by poliovirus, binds to the viral internal ribosome entry site (IRES) and stimulates the IRES-mediated translation (PubMed:12414943, PubMed:24371074). Also plays a role in initiation of viral RNA replication in concert with the viral protein 3CD (PubMed:12414943).2 Publications

GO - Molecular functioni

  • DNA binding Source: UniProtKB-KW
  • enzyme binding Source: UniProtKB
  • RNA binding Source: UniProtKB
  • ubiquitin protein ligase binding Source: UniProtKB

GO - Biological processi

  • defense response to virus Source: UniProtKB-KW
  • innate immune response Source: UniProtKB-KW
  • IRES-dependent viral translational initiation Source: UniProtKB
  • mRNA metabolic process Source: UniProtKB
  • mRNA splicing, via spliceosome Source: Reactome
  • negative regulation of defense response to virus Source: UniProtKB
  • negative regulation of type I interferon production Source: Reactome
  • proteasome-mediated ubiquitin-dependent protein catabolic process Source: UniProtKB
  • RNA metabolic process Source: Reactome
  • viral RNA genome replication Source: UniProtKB

Keywordsi

Molecular functionDNA-binding, Ribonucleoprotein, RNA-binding
Biological processAntiviral defense, Immunity, Innate immunity, Viral RNA replication

Enzyme and pathway databases

ReactomeiR-HSA-72163 mRNA Splicing - Major Pathway
R-HSA-72203 Processing of Capped Intron-Containing Pre-mRNA
R-HSA-936440 Negative regulators of DDX58/IFIH1 signaling

Names & Taxonomyi

Protein namesi
Recommended name:
Poly(rC)-binding protein 2
Alternative name(s):
Alpha-CP2
Heterogeneous nuclear ribonucleoprotein E2
Short name:
hnRNP E2
Gene namesi
Name:PCBP2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 12

Organism-specific databases

EuPathDBiHostDB:ENSG00000197111.15
HGNCiHGNC:8648 PCBP2
MIMi601210 gene
neXtProtiNX_Q15366

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi5094
OpenTargetsiENSG00000197111
PharmGKBiPA32987

Polymorphism and mutation databases

BioMutaiPCBP2
DMDMi6707736

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000500901 – 365Poly(rC)-binding protein 2Add BLAST365

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki115Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei173PhosphoserineCombined sources1
Cross-linki185Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei189PhosphoserineCombined sources1
Modified residuei272PhosphoserineCombined sources1
Cross-linki322Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei364PhosphoserineCombined sources1
Modified residuei365PhosphoserineCombined sources1

Post-translational modificationi

Phosphorylated. The non-phosphorylated form(s) exhibited the strongest poly(rC)-binding activity.
(Microbial infection) Proteolyticaly cleaved by picornavirus proteinase 3CD.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei253 – 254Cleavage, by viral proteinase 3CD1 Publication2

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ15366
MaxQBiQ15366
PaxDbiQ15366
PeptideAtlasiQ15366
PRIDEiQ15366
ProteomicsDBi60542
60543 [Q15366-2]
60544 [Q15366-3]
60545 [Q15366-4]
60546 [Q15366-5]

PTM databases

iPTMnetiQ15366
PhosphoSitePlusiQ15366
SwissPalmiQ15366

Expressioni

Tissue specificityi

Detected in all tissues examined.

Gene expression databases

BgeeiENSG00000197111
CleanExiHS_PCBP2
ExpressionAtlasiQ15366 baseline and differential
GenevisibleiQ15366 HS

Organism-specific databases

HPAiHPA038356

Interactioni

Subunit structurei

Identified in a mRNP complex, at least composed of DHX9, DDX3X, ELAVL1, HNRNPU, IGF2BP1, ILF3, PABPC1, PCBP2, PTBP2, STAU1, STAU2, SYNCRIP and YBX1. Interacts with IFIH1 and RNF135. Interacts with MAVS (via C-terminus) and ITCH (via WW domains).5 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

  • enzyme binding Source: UniProtKB
  • ubiquitin protein ligase binding Source: UniProtKB

Protein-protein interaction databases

BioGridi111127, 96 interactors
DIPiDIP-58934N
IntActiQ15366, 51 interactors
MINTiQ15366
STRINGi9606.ENSP00000352438

Structurei

Secondary structure

1365
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi14 – 21Combined sources8
Helixi22 – 29Combined sources8
Helixi31 – 33Combined sources3
Helixi34 – 43Combined sources10
Beta strandi46 – 49Combined sources4
Beta strandi55 – 63Combined sources9
Helixi65 – 80Combined sources16
Beta strandi89 – 91Combined sources3
Beta strandi97 – 105Combined sources9
Helixi106 – 113Combined sources8
Helixi115 – 117Combined sources3
Helixi118 – 127Combined sources10
Beta strandi128 – 131Combined sources4
Beta strandi143 – 150Combined sources8
Helixi152 – 168Combined sources17
Beta strandi288 – 295Combined sources8
Helixi296 – 303Combined sources8
Helixi305 – 307Combined sources3
Helixi308 – 317Combined sources10
Beta strandi320 – 323Combined sources4
Beta strandi331 – 339Combined sources9
Helixi341 – 355Combined sources15

3D structure databases

ProteinModelPortaliQ15366
SMRiQ15366
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ15366

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini13 – 75KH 1PROSITE-ProRule annotationAdd BLAST63
Domaini97 – 162KH 2PROSITE-ProRule annotationAdd BLAST66
Domaini287 – 351KH 3PROSITE-ProRule annotationAdd BLAST65

Domaini

The KH domains mediates poly(C) binding.

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG2190 Eukaryota
ENOG410XNN8 LUCA
GeneTreeiENSGT00760000119144
HOGENOMiHOG000182823
HOVERGENiHBG053520
InParanoidiQ15366
KOiK13162
OMAiMVASKDM
OrthoDBiEOG091G0DHI
PhylomeDBiQ15366
TreeFamiTF318292

Family and domain databases

Gene3Di3.30.1370.10, 3 hits
InterProiView protein in InterPro
IPR004087 KH_dom
IPR004088 KH_dom_type_1
IPR036612 KH_dom_type_1_sf
PfamiView protein in Pfam
PF00013 KH_1, 3 hits
SMARTiView protein in SMART
SM00322 KH, 3 hits
SUPFAMiSSF54791 SSF54791, 3 hits
PROSITEiView protein in PROSITE
PS50084 KH_TYPE_1, 3 hits

Sequences (8)i

Sequence statusi: Complete.

This entry describes 8 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q15366-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDTGVIEGGL NVTLTIRLLM HGKEVGSIIG KKGESVKKMR EESGARINIS
60 70 80 90 100
EGNCPERIIT LAGPTNAIFK AFAMIIDKLE EDISSSMTNS TAASRPPVTL
110 120 130 140 150
RLVVPASQCG SLIGKGGCKI KEIRESTGAQ VQVAGDMLPN STERAITIAG
160 170 180 190 200
IPQSIIECVK QICVVMLETL SQSPPKGVTI PYRPKPSSSP VIFAGGQDRY
210 220 230 240 250
STGSDSASFP HTTPSMCLNP DLEGPPLEAY TIQGQYAIPQ PDLTKLHQLA
260 270 280 290 300
MQQSHFPMTH GNTGFSGIES SSPEVKGYWG LDASAQTTSH ELTIPNDLIG
310 320 330 340 350
CIIGRQGAKI NEIRQMSGAQ IKIANPVEGS TDRQVTITGS AASISLAQYL
360
INVRLSSETG GMGSS
Length:365
Mass (Da):38,580
Last modified:November 1, 1996 - v1
Checksum:i43F035D76FDC2C63
GO
Isoform 2 (identifier: Q15366-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     279-279: W → WA

Note: No experimental confirmation available.
Show »
Length:366
Mass (Da):38,651
Checksum:i3464C973504D90DE
GO
Isoform 3 (identifier: Q15366-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     169-172: Missing.
     279-279: W → WA

Show »
Length:362
Mass (Da):38,222
Checksum:i70C8AF710E3BF3C0
GO
Isoform 4 (identifier: Q15366-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     169-172: Missing.
     198-228: Missing.
     279-279: W → WA

Note: No experimental confirmation available.
Show »
Length:331
Mass (Da):34,917
Checksum:iE831D4BF718C26E3
GO
Isoform 5 (identifier: Q15366-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     198-228: Missing.
     279-279: W → WA

Note: No experimental confirmation available.
Show »
Length:335
Mass (Da):35,347
Checksum:iBD1A8AA65EABF0BF
GO
Isoform 6 (identifier: Q15366-6) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     169-172: Missing.

Note: No experimental confirmation available.
Show »
Length:361
Mass (Da):38,151
Checksum:i4F96378919BF3215
GO
Isoform 7 (identifier: Q15366-7) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     169-172: Missing.
     198-228: Missing.
     267-279: GIESSSPEVKGYW → A

Note: No experimental confirmation available. Gene prediction based on EST data.
Show »
Length:318
Mass (Da):33,497
Checksum:iB5B41191CFD1B171
GO
Isoform 8 (identifier: Q15366-8) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     198-228: Missing.
     267-279: GIESSSPEVKGYW → A

Note: No experimental confirmation available.
Show »
Length:322
Mass (Da):33,926
Checksum:i7DDD51D4F8BEB977
GO

Sequence cautioni

The sequence BAD92062 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_043161169 – 172Missing in isoform 3, isoform 4, isoform 6 and isoform 7. 4 Publications4
Alternative sequenceiVSP_043362198 – 228Missing in isoform 4, isoform 5, isoform 7 and isoform 8. 3 PublicationsAdd BLAST31
Alternative sequenceiVSP_054045267 – 279GIESS…VKGYW → A in isoform 7 and isoform 8. 1 PublicationAdd BLAST13
Alternative sequenceiVSP_042833279W → WA in isoform 2, isoform 3, isoform 4 and isoform 5. 3 Publications1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X78136 mRNA Translation: CAA55015.1
AB188306 mRNA Translation: BAD36897.1
AK292141 mRNA Translation: BAF84830.1
AK302067 mRNA Translation: BAG63457.1
AB208825 mRNA Translation: BAD92062.1 Different initiation.
AC023509 Genomic DNA No translation available.
AC068889 Genomic DNA No translation available.
CH471054 Genomic DNA Translation: EAW96706.1
CH471054 Genomic DNA Translation: EAW96707.1
CH471054 Genomic DNA Translation: EAW96709.1
CH471054 Genomic DNA Translation: EAW96711.1
BC001155 mRNA Translation: AAH01155.1
BC071942 mRNA Translation: AAH71942.1
BC107688 mRNA Translation: AAI07689.1
CCDSiCCDS44900.1 [Q15366-2]
CCDS44901.1 [Q15366-1]
CCDS44902.1 [Q15366-5]
CCDS44903.1 [Q15366-4]
CCDS44904.1 [Q15366-7]
CCDS55830.1 [Q15366-6]
CCDS8859.1 [Q15366-3]
PIRiS65679 S42471
RefSeqiNP_001092090.1, NM_001098620.2 [Q15366-4]
NP_001122383.1, NM_001128911.1 [Q15366-1]
NP_001122384.1, NM_001128912.1 [Q15366-6]
NP_001122385.1, NM_001128913.1 [Q15366-5]
NP_001122386.1, NM_001128914.1 [Q15366-7]
NP_005007.2, NM_005016.5 [Q15366-2]
NP_114366.1, NM_031989.4 [Q15366-3]
UniGeneiHs.546271

Genome annotation databases

EnsembliENST00000359282; ENSP00000352228; ENSG00000197111 [Q15366-4]
ENST00000359462; ENSP00000352438; ENSG00000197111 [Q15366-2]
ENST00000437231; ENSP00000390304; ENSG00000197111 [Q15366-7]
ENST00000439930; ENSP00000408949; ENSG00000197111 [Q15366-1]
ENST00000447282; ENSP00000394116; ENSG00000197111 [Q15366-5]
ENST00000455667; ENSP00000388008; ENSG00000197111 [Q15366-7]
ENST00000546463; ENSP00000448762; ENSG00000197111 [Q15366-3]
ENST00000548933; ENSP00000449062; ENSG00000197111 [Q15366-5]
ENST00000552296; ENSP00000448927; ENSG00000197111 [Q15366-6]
ENST00000552819; ENSP00000449070; ENSG00000197111 [Q15366-8]
GeneIDi5094
KEGGihsa:5094
UCSCiuc001sdb.5 human [Q15366-1]
uc058oqk.1 human

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiPCBP2_HUMAN
AccessioniPrimary (citable) accession number: Q15366
Secondary accession number(s): A8K7X6
, B4DXP5, F8VYL7, G3V0E8, I6L8F9, Q32Q82, Q59HD4, Q68Y55, Q6IPF4, Q6PKG5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1996
Last modified: July 18, 2018
This is version 181 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 12
    Human chromosome 12: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

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