UniProtKB - Q15287 (RNPS1_HUMAN)
RNA-binding protein with serine-rich domain 1
RNPS1
Functioni
GO - Molecular functioni
- mRNA 3'-UTR binding Source: UniProtKB
- RNA binding Source: UniProtKB
GO - Biological processi
- mRNA 3'-end processing Source: Reactome
- mRNA export from nucleus Source: Reactome
- mRNA splicing, via spliceosome Source: GO_Central
- negative regulation of mRNA splicing, via spliceosome Source: UniProtKB
- nuclear-transcribed mRNA catabolic process, nonsense-mediated decay Source: UniProtKB
- positive regulation of apoptotic process Source: UniProtKB
- regulation of alternative mRNA splicing, via spliceosome Source: UniProtKB
- RNA export from nucleus Source: Reactome
- RNA splicing Source: GO_Central
- transcription, DNA-templated Source: ProtInc
Keywordsi
Molecular function | RNA-binding |
Biological process | mRNA processing, mRNA splicing, Nonsense-mediated mRNA decay |
Enzyme and pathway databases
PathwayCommonsi | Q15287 |
Reactomei | R-HSA-159236, Transport of Mature mRNA derived from an Intron-Containing Transcript R-HSA-72163, mRNA Splicing - Major Pathway R-HSA-72187, mRNA 3'-end processing R-HSA-73856, RNA Polymerase II Transcription Termination R-HSA-9010553, Regulation of expression of SLITs and ROBOs R-HSA-975957, Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) |
Protein family/group databases
TCDBi | 3.A.18.1.1, the nuclear mrna exporter (mrna-e) family |
Names & Taxonomyi
Protein namesi | Recommended name: RNA-binding protein with serine-rich domain 1Alternative name(s): SR-related protein LDC2 |
Gene namesi | Name:RNPS1 Synonyms:LDC2 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
EuPathDBi | HostDB:ENSG00000205937.11 |
HGNCi | HGNC:10080, RNPS1 |
MIMi | 606447, gene |
neXtProti | NX_Q15287 |
Subcellular locationi
Nucleus
Other locations
Note: Nucleocytoplasmic shuttling protein. Colocalizes with the core EJC, ALYREF/THOC4, NXF1 and UAP56 in the nucleus and nuclear speckles.
Cytosol
- cytosol Source: Reactome
Nucleus
- nuclear speck Source: UniProtKB-SubCell
- nucleoplasm Source: HPA
- nucleus Source: UniProtKB
Other locations
- ASAP complex Source: UniProtKB
- cytoplasm Source: UniProtKB
Keywords - Cellular componenti
Cytoplasm, NucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 53 | S → A: Abolishes phosphorylation by CSNK2A1 and partially reduces splicing stimulation. Does not abolish interaction with CSNK2A1 and subcellular localization. 1 Publication | 1 | |
Mutagenesisi | 53 | S → E: Partially reduces splicing stimulation. Does not abolish interaction with CSNK2A1 and subcellular localization. 1 Publication | 1 | |
Mutagenesisi | 171 | N → R: Impairs interaction with SAP18. 1 Publication | 1 | |
Mutagenesisi | 205 | Y → A: Abolishes exon-skipping. 1 Publication | 1 | |
Mutagenesisi | 207 | Y → A: Abolishes exon-skipping. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 10921 |
OpenTargetsi | ENSG00000205937 |
PharmGKBi | PA34453 |
Miscellaneous databases
Pharosi | Q15287, Tbio |
Polymorphism and mutation databases
BioMutai | RNPS1 |
DMDMi | 74754492 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000081816 | 1 – 305 | RNA-binding protein with serine-rich domain 1Add BLAST | 305 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Cross-linki | 7 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources | ||
Cross-linki | 15 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources | ||
Modified residuei | 53 | Phosphoserine; by CK21 Publication | 1 | |
Modified residuei | 155 | PhosphoserineCombined sources | 1 | |
Modified residuei | 157 | PhosphoserineCombined sources | 1 | |
Modified residuei | 161 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 218 | N6-acetyllysineCombined sources | 1 |
Post-translational modificationi
Keywords - PTMi
Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
EPDi | Q15287 |
jPOSTi | Q15287 |
MassIVEi | Q15287 |
MaxQBi | Q15287 |
PaxDbi | Q15287 |
PeptideAtlasi | Q15287 |
PRIDEi | Q15287 |
ProteomicsDBi | 60514 [Q15287-1] 60515 [Q15287-2] 60516 [Q15287-3] |
PTM databases
iPTMneti | Q15287 |
PhosphoSitePlusi | Q15287 |
SwissPalmi | Q15287 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSG00000205937, Expressed in cortical plate and 215 other tissues |
ExpressionAtlasi | Q15287, baseline and differential |
Genevisiblei | Q15287, HS |
Organism-specific databases
HPAi | ENSG00000205937, Low tissue specificity |
Interactioni
Subunit structurei
Found in mRNA splicing-dependent exon junction complexes (EJC).
Found in a post-splicing complex with NXF1, RBM8A, UPF1, UPF2, UPF3A, UPF3B and RNPS1.
Component of the heterotrimeric ASAP (apoptosis- and splicing-associated protein) and PSAP complexes consisting of RNPS1, SAP18 and either ACIN1 or PNN, respectively; the ASAP and PSAP complexes probably are formed mutually exclusive.
Component of the active spliceosome. Associates with polysomes.
Interacts with the cleaved p110 isoform of CDC2L1, CSNK2A1, PNN, SART3, SRP54, SRRM1 and TRA2B/SFRS10.
14 PublicationsBinary interactionsi
Hide detailsQ15287
Isoform 1 [Q15287-1]
With | #Exp. | IntAct |
---|---|---|
ACIN1 - isoform 2 [Q9UKV3-2] | 4 | EBI-15972541,EBI-5279966 |
PNN [Q9H307] | 3 | EBI-15972541,EBI-681904 |
Acn [Q9VJ12] from Drosophila melanogaster. | 4 | EBI-15972541,EBI-3418150 |
Protein-protein interaction databases
BioGRIDi | 116125, 244 interactors |
CORUMi | Q15287 |
DIPi | DIP-32943N |
IntActi | Q15287, 132 interactors |
MINTi | Q15287 |
STRINGi | 9606.ENSP00000457723 |
Miscellaneous databases
RNActi | Q15287, protein |
Structurei
Secondary structure
3D structure databases
SMRi | Q15287 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 161 – 240 | RRMPROSITE-ProRule annotationAdd BLAST | 80 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 220 | Necessary for interaction with the cleaved p110 isoform of CDC2L1Add BLAST | 220 | |
Regioni | 1 – 161 | Necessary for interaction with SRP54, nuclear localization and exon-skipping1 PublicationAdd BLAST | 161 | |
Regioni | 69 – 121 | Necessary for interactions with UPF2 and UPF3B and UPF2-dependent NMDAdd BLAST | 53 | |
Regioni | 156 – 242 | Necessary for interaction with PNN and exon-skippingAdd BLAST | 87 | |
Regioni | 159 – 244 | Interaction with SAP18 and ACIN11 PublicationAdd BLAST | 86 | |
Regioni | 238 – 305 | Necessary for interaction with TRA2B, nuclear localization and exon-skipping1 PublicationAdd BLAST | 68 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 7 – 64 | Lys-richAdd BLAST | 58 | |
Compositional biasi | 67 – 141 | Ser-richAdd BLAST | 75 | |
Compositional biasi | 128 – 154 | Arg-richAdd BLAST | 27 | |
Compositional biasi | 241 – 298 | Arg/Pro-richAdd BLAST | 58 |
Domaini
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG4209, Eukaryota |
GeneTreei | ENSGT00730000111029 |
InParanoidi | Q15287 |
OMAi | PNFGRGV |
PhylomeDBi | Q15287 |
TreeFami | TF314165 |
Family and domain databases
CDDi | cd12365, RRM_RNPS1, 1 hit |
Gene3Di | 3.30.70.330, 1 hit |
InterProi | View protein in InterPro IPR012677, Nucleotide-bd_a/b_plait_sf IPR035979, RBD_domain_sf IPR034201, RNPS1_RRM IPR000504, RRM_dom |
Pfami | View protein in Pfam PF00076, RRM_1, 1 hit |
SMARTi | View protein in SMART SM00360, RRM, 1 hit |
SUPFAMi | SSF54928, SSF54928, 1 hit |
PROSITEi | View protein in PROSITE PS50102, RRM, 1 hit |
s (3+)i Sequence
Sequence statusi: Complete.
This entry describes 3 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 3 described isoforms and 14 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MDLSGVKKKS LLGVKENNKK SSTRAPSPTK RKDRSDEKSK DRSKDKGATK
60 70 80 90 100
ESSEKDRGRD KTRKRRSASS GSSSTRSRSS STSSSGSSTS TGSSSGSSSS
110 120 130 140 150
SASSRSGSSS TSRSSSSSSS SGSPSPSRRR HDNRRRSRSK SKPPKRDEKE
160 170 180 190 200
RKRRSPSPKP TKVHIGRLTR NVTKDHIMEI FSTYGKIKMI DMPVERMHPH
210 220 230 240 250
LSKGYAYVEF ENPDEAEKAL KHMDGGQIDG QEITATAVLA PWPRPPPRRF
260 270 280 290 300
SPPRRMLPPP PMWRRSPPRM RRRSRSPRRR SPVRRRSRSP GRRRHRSRSS
SNSSR
Computationally mapped potential isoform sequencesi
There are 14 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketH3BMM9 | H3BMM9_HUMAN | RNA-binding protein with serine-ric... | RNPS1 | 284 | Annotation score: | ||
H3BTC0 | H3BTC0_HUMAN | RNA-binding protein with serine-ric... | RNPS1 | 302 | Annotation score: | ||
H3BMS0 | H3BMS0_HUMAN | RNA-binding protein with serine-ric... | RNPS1 | 257 | Annotation score: | ||
H3BV80 | H3BV80_HUMAN | RNA-binding protein with serine-ric... | RNPS1 | 211 | Annotation score: | ||
H3BPG5 | H3BPG5_HUMAN | RNA-binding protein with serine-ric... | RNPS1 hCG_1989969 | 128 | Annotation score: | ||
H3BP82 | H3BP82_HUMAN | RNA-binding protein with serine-ric... | RNPS1 | 98 | Annotation score: | ||
H3BTR6 | H3BTR6_HUMAN | RNA-binding protein with serine-ric... | RNPS1 | 127 | Annotation score: | ||
H3BUL0 | H3BUL0_HUMAN | RNA-binding protein with serine-ric... | RNPS1 | 169 | Annotation score: | ||
H3BNI3 | H3BNI3_HUMAN | RNA-binding protein with serine-ric... | RNPS1 | 85 | Annotation score: | ||
H3BTY0 | H3BTY0_HUMAN | RNA-binding protein with serine-ric... | RNPS1 | 150 | Annotation score: | ||
There are more potential isoformsShow all |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 32 | K → E in BAG56859 (PubMed:14702039).Curated | 1 | |
Sequence conflicti | 66 | R → G in AAC39791 (PubMed:9580558).Curated | 1 | |
Sequence conflicti | 249 | R → G in BAG56859 (PubMed:14702039).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_016243 | 2 – 24 | Missing in isoform 2 and isoform 3. 2 PublicationsAdd BLAST | 23 | |
Alternative sequenceiVSP_037601 | 69 – 82 | Missing in isoform 3. 1 PublicationAdd BLAST | 14 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L37368 mRNA Translation: AAA92859.1 AF015608 mRNA Translation: AAC39791.1 AF274003 mRNA Translation: AAL56665.1 AK289955 mRNA Translation: BAF82644.1 AK293343 mRNA Translation: BAG56859.1 AK303100 mRNA Translation: BAG64209.1 AK316132 mRNA Translation: BAH14503.1 AC009065 Genomic DNA No translation available. CH471112 Genomic DNA Translation: EAW85516.1 BC001659 mRNA Translation: AAH01659.1 BC001838 mRNA Translation: AAH01838.1 BC108316 mRNA Translation: AAI08317.1 AF247662 mRNA Translation: AAF72519.1 |
CCDSi | CCDS10465.1 [Q15287-1] CCDS66907.1 [Q15287-2] |
PIRi | JC4525 |
RefSeqi | NP_001273554.1, NM_001286625.1 [Q15287-1] NP_001273555.1, NM_001286626.1 [Q15287-2] NP_001273556.1, NM_001286627.1 NP_006702.1, NM_006711.4 [Q15287-1] NP_542161.1, NM_080594.3 [Q15287-1] XP_005255105.1, XM_005255048.1 [Q15287-1] XP_005255106.1, XM_005255049.3 [Q15287-1] XP_016878363.1, XM_017022874.1 |
Genome annotation databases
Ensembli | ENST00000301730; ENSP00000301730; ENSG00000205937 [Q15287-1] ENST00000320225; ENSP00000315859; ENSG00000205937 [Q15287-1] ENST00000397086; ENSP00000380275; ENSG00000205937 [Q15287-1] ENST00000565678; ENSP00000457723; ENSG00000205937 [Q15287-1] ENST00000566458; ENSP00000456352; ENSG00000205937 [Q15287-2] ENST00000568631; ENSP00000457820; ENSG00000205937 [Q15287-1] |
GeneIDi | 10921 |
KEGGi | hsa:10921 |
UCSCi | uc002cpt.5, human [Q15287-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L37368 mRNA Translation: AAA92859.1 AF015608 mRNA Translation: AAC39791.1 AF274003 mRNA Translation: AAL56665.1 AK289955 mRNA Translation: BAF82644.1 AK293343 mRNA Translation: BAG56859.1 AK303100 mRNA Translation: BAG64209.1 AK316132 mRNA Translation: BAH14503.1 AC009065 Genomic DNA No translation available. CH471112 Genomic DNA Translation: EAW85516.1 BC001659 mRNA Translation: AAH01659.1 BC001838 mRNA Translation: AAH01838.1 BC108316 mRNA Translation: AAI08317.1 AF247662 mRNA Translation: AAF72519.1 |
CCDSi | CCDS10465.1 [Q15287-1] CCDS66907.1 [Q15287-2] |
PIRi | JC4525 |
RefSeqi | NP_001273554.1, NM_001286625.1 [Q15287-1] NP_001273555.1, NM_001286626.1 [Q15287-2] NP_001273556.1, NM_001286627.1 NP_006702.1, NM_006711.4 [Q15287-1] NP_542161.1, NM_080594.3 [Q15287-1] XP_005255105.1, XM_005255048.1 [Q15287-1] XP_005255106.1, XM_005255049.3 [Q15287-1] XP_016878363.1, XM_017022874.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
4A8X | X-ray | 1.90 | A | 159-244 | [»] | |
SMRi | Q15287 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 116125, 244 interactors |
CORUMi | Q15287 |
DIPi | DIP-32943N |
IntActi | Q15287, 132 interactors |
MINTi | Q15287 |
STRINGi | 9606.ENSP00000457723 |
Protein family/group databases
TCDBi | 3.A.18.1.1, the nuclear mrna exporter (mrna-e) family |
PTM databases
iPTMneti | Q15287 |
PhosphoSitePlusi | Q15287 |
SwissPalmi | Q15287 |
Polymorphism and mutation databases
BioMutai | RNPS1 |
DMDMi | 74754492 |
Proteomic databases
EPDi | Q15287 |
jPOSTi | Q15287 |
MassIVEi | Q15287 |
MaxQBi | Q15287 |
PaxDbi | Q15287 |
PeptideAtlasi | Q15287 |
PRIDEi | Q15287 |
ProteomicsDBi | 60514 [Q15287-1] 60515 [Q15287-2] 60516 [Q15287-3] |
Protocols and materials databases
Antibodypediai | 23681, 70 antibodies |
DNASUi | 10921 |
Genome annotation databases
Ensembli | ENST00000301730; ENSP00000301730; ENSG00000205937 [Q15287-1] ENST00000320225; ENSP00000315859; ENSG00000205937 [Q15287-1] ENST00000397086; ENSP00000380275; ENSG00000205937 [Q15287-1] ENST00000565678; ENSP00000457723; ENSG00000205937 [Q15287-1] ENST00000566458; ENSP00000456352; ENSG00000205937 [Q15287-2] ENST00000568631; ENSP00000457820; ENSG00000205937 [Q15287-1] |
GeneIDi | 10921 |
KEGGi | hsa:10921 |
UCSCi | uc002cpt.5, human [Q15287-1] |
Organism-specific databases
CTDi | 10921 |
DisGeNETi | 10921 |
EuPathDBi | HostDB:ENSG00000205937.11 |
GeneCardsi | RNPS1 |
HGNCi | HGNC:10080, RNPS1 |
HPAi | ENSG00000205937, Low tissue specificity |
MIMi | 606447, gene |
neXtProti | NX_Q15287 |
OpenTargetsi | ENSG00000205937 |
PharmGKBi | PA34453 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG4209, Eukaryota |
GeneTreei | ENSGT00730000111029 |
InParanoidi | Q15287 |
OMAi | PNFGRGV |
PhylomeDBi | Q15287 |
TreeFami | TF314165 |
Enzyme and pathway databases
PathwayCommonsi | Q15287 |
Reactomei | R-HSA-159236, Transport of Mature mRNA derived from an Intron-Containing Transcript R-HSA-72163, mRNA Splicing - Major Pathway R-HSA-72187, mRNA 3'-end processing R-HSA-73856, RNA Polymerase II Transcription Termination R-HSA-9010553, Regulation of expression of SLITs and ROBOs R-HSA-975957, Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) |
Miscellaneous databases
BioGRID-ORCSi | 10921, 691 hits in 854 CRISPR screens |
ChiTaRSi | RNPS1, human |
GeneWikii | RNPS1 |
GenomeRNAii | 10921 |
Pharosi | Q15287, Tbio |
PROi | PR:Q15287 |
RNActi | Q15287, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000205937, Expressed in cortical plate and 215 other tissues |
ExpressionAtlasi | Q15287, baseline and differential |
Genevisiblei | Q15287, HS |
Family and domain databases
CDDi | cd12365, RRM_RNPS1, 1 hit |
Gene3Di | 3.30.70.330, 1 hit |
InterProi | View protein in InterPro IPR012677, Nucleotide-bd_a/b_plait_sf IPR035979, RBD_domain_sf IPR034201, RNPS1_RRM IPR000504, RRM_dom |
Pfami | View protein in Pfam PF00076, RRM_1, 1 hit |
SMARTi | View protein in SMART SM00360, RRM, 1 hit |
SUPFAMi | SSF54928, SSF54928, 1 hit |
PROSITEi | View protein in PROSITE PS50102, RRM, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | RNPS1_HUMAN | |
Accessioni | Q15287Primary (citable) accession number: Q15287 Secondary accession number(s): A8K1P0 Q9NYG3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 22, 2005 |
Last sequence update: | November 1, 1996 | |
Last modified: | December 2, 2020 | |
This is version 201 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families - Human chromosome 16
Human chromosome 16: entries, gene names and cross-references to MIM